LOCUS       Exported                9684 bp ds-DNA     circular SYN 22-JUL-2024
DEFINITION  mR1-28.
ACCESSION   mR1-28
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..9684
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..826
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    complement(828..962)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(982..1117)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(1141..1163)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             1170..1209
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1258..1288
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1296..1312
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(1697..1752)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1823..1894)
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     CDS             complement(2312..3812)
                     /label=SEAP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
     misc_feature    complement(3825..4287)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4396..4414
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.4"
                     /note="/other=promoter"
     CDS             complement(4426..5145)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     misc_feature    complement(5153..5179)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=34.6"
                     /note="/other=misc_feature"
     promoter        complement(5186..5382)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      5200..5335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5400..8515
                     /label=mRegion1
     misc_feature    6781..7062
                     /label=RefinedRegion1
     misc_feature    complement(8435..8452)
                     /label=HA-L (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=misc_feature"
     promoter        8549..8653
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             8654..9514
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagatct cagtggtatt tgtgagccag ggcattggcc acaccagcca
     1861 ccaccttctg ataggcagcc tgcacctgag gagtgcggcc ggccgcgata tcctcgaccc
     1921 cactgggctg taggtggtgg ctggatggcc tgagccccct cctttcctgg ggtgcaggca
     1981 gccacagggc acaacctgca gtccctgggg ttggggtagg gagtccgaga gcagccaagg
     2041 ggcaggtttg gtgtcatggg ctcagtcccc agagcgcacg gggaggggaa ggttccatgg
     2101 caggacctct gtatagggac tccggggatg acgactcggg ctggaggcca ggcaggacga
     2161 caggaggtgg ggagcaggag aactccggga tggggaagca ggagccccag ggacgggaca
     2221 ctcagggagc agtggccgtc tccagcagca gcagggtccc ggccagcaga ggaagcaacg
     2281 cggggaccac ggaccgcccc gggtgcgcgg cgtcggtggt gccggcgggg ggcgccaggt
     2341 cgcaggcggt gtagggctcc aggcaggcgg cgaaggccat gacgtgcgct atgaaggtct
     2401 gctcctgcac gccgtgaacc aggtgcgcct gcgggccgcg cgcgaacacc gccacgtcct
     2461 cgcctgcgtg ggtctcttcg tccaggggca ctgctgactg ctgccgatac tcggggctcc
     2521 cgctctcgct ctcggtaaca tccggccggg cgccgtcctt gagcacatag cctggaccgt
     2581 ttccgtatag gaggaccgtg taggccttcc tgtcccgggc cttgccaggg gccagcccga
     2641 agatggagct ccctcgcagg gggtagcctc cgaaggagaa gacgtgggag tggtcggcag
     2701 tgacgaggct cagcgtgtcc tcctcgctgg tgagctggcc cgccctctca atggcgtcgt
     2761 cgaacatgat cgtctcagtc agtgcccggt aagccctgct ttcatgatga ccatggtcga
     2821 tgcgaccacc ctccacgaag aggaagaagc cgcgggggtt cctgctcagc aggcgcaggg
     2881 cagcctctgt catctccatc agggaggggt ccagtgtgga gtctcggtgg atctcgtatt
     2941 tcatgtctcc aggctcaaag agacccatga gatgggtcac agacgggtcc agggaagcct
     3001 gcatgagctc agtgcggttc cacacatacc gggcaccctg gcgcttcgcc agccattcct
     3061 gcaccagatt cttcccgtcc agcctggtcc caccttggct gtagtcatct gggtactcag
     3121 ggtctggggt tcccatgcga aacatgtact ttcggcctcc acctaggatc acgtcaatgt
     3181 ccatgttgga gatgagctgc gtagcgatgt cctggcaccc ctcctggcgg gccgaggcag
     3241 gcacgtcggc gtccgagtac cagttgcggt tcaccgtgtg ggcgtaggtg ccggctggcg
     3301 aggcgtgctg cactcgtgtg gtggttacca ctcccactga cttccctgct ttcttggccc
     3361 gattcatcac ggagatgacc tcgttgccgc gtgtcgtgtt gcactggtta aagcgggcgg
     3421 ctgcactcaa gccaatggtc tggaagttgc ccttgacccc gcacaggtag gccgtggctg
     3481 tggctccact gtctggcaca tgtttgtcta cattgtatgt cttggacaga gccacatatg
     3541 ggaagcggtc catggccagg ggtatctcag gccccagttt gtccttcttc tgccctttta
     3601 ggatcctggc agctgtcacc gtagacaccc ccatcccatc gcccaggaag atgatgaggt
     3661 tcttggcggc tgtctgtgca ggctgcagct tcttggcggc acccagggcc tcggctgcct
     3721 cgcggttcca gaagtccggg ttctcctcct caactgggat gatgcccagg gagagctgta
     3781 gcctcaggcc cagcagcagc agcagcagca gcctcgaccc catattatca tcgtgttttt
     3841 caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa cctcgactaa
     3901 acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg gggtaccttc
     3961 tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac tctttccaca
     4021 actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt atcttataca
     4081 cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg cctgcaaagg
     4141 gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc cttcacgaca
     4201 ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat gctcgtcaag
     4261 aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat ggtggaaaat
     4321 aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt aacgttaggg
     4381 gggggggagg gagagggggg gggggggggg gcggaattgg ccgctttact tgtacagctc
     4441 gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg
     4501 cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag
     4561 cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct
     4621 gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc
     4681 ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt
     4741 gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc
     4801 gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc
     4861 ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg
     4921 gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac
     4981 gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc
     5041 ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag
     5101 gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg cgaccggtgg
     5161 atcccgggcc cgcggtacca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     5221 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     5281 ttagtcagcg atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     5341 gagttagggg cgggactatg gttgctgact aattgagatg cagatcgcag atctcgagct
     5401 ggcatgcatg tatatgtgta ctggcatgcg tgtatatatg tactggcatg tgtgtaaacc
     5461 ttgcgcttag gaggctgaac aggaggactg tgagttcaag gacagcctag actatacact
     5521 gagtctctgg ctaaacaaat actgtaaaca cagcatctat cttgataaac aacccattaa
     5581 acatacctat ggacacaaat aatatattca ccaatttttt ttatatgcta ttcctgccag
     5641 cacacagtag gcacttccta agggcctact gtccctttca tctccagggc acaacattaa
     5701 caattctgtt tgcccagcca cctccctttc cactgtgacc cttgatcagc aagcacaata
     5761 aatgtttgct aggtgactga tttataccag ggatgaggga acgtctccaa gcttccacag
     5821 aagtgaaaga caagtggcct ccatcgcccc acaagggaag atgatgtcag gtgccagggt
     5881 ggagatggtg aacatactcg gtgtgctgga gtcccagcag ccaagctcac cctgtcccag
     5941 ggtcaaaagt attcagctgc caggaccata tcagaaatgt aatctcagta cccaaggtct
     6001 gtcctaaggc ctggctcctt aggccttgca cctcccacct aagttggaca gggccctttc
     6061 ttccacttct ttagttggag atgggtgttt aactcatatg atgagccatg tattgtatca
     6121 gggctctttg tgagtggaga gggacacctg agccctgggt acccaaaggt gtagagagat
     6181 ggggacacag atgtagccag aatacctcag cctccagcac ctggcatgtt gtagagcacc
     6241 aattcacagt cttcaggggt gtactgaaag ctggggactg tcaggagcct agaggtaccg
     6301 actccatacc ctgagcagtt cttaggcgaa gccgctgagt tgtgtgtggc tcgcctccac
     6361 tgttacacca ctgaacacac tgactagtgt ggaccaggca cttcccaagg agagtctcag
     6421 aggtctggga aggccttgaa aaagtcttct caatttttag aatggaaaag cagcccaaag
     6481 aagtctaagt catttccagt gccacagagt tcacatccgc tgggagcctc aactcccggc
     6541 ctcctcttgg ctccactcct gcccaggatc tcaggggtca ggggctgctc ttgagggttc
     6601 ttcatggctc tccattccta ctgatccact tccctaaagt cccaccggac tctgagctct
     6661 acatctccct gtccactcac tcagttcctc aggaggtcct ggagacccca gtagtgggga
     6721 gagaggtcct tggtgcctct tagctgtccg ctagctcaga gctgtaggga ggctgttgcg
     6781 gtgcacagca ctgatcctaa ccaagtttgc cagcccagct tttctcatgg ctcccacaca
     6841 atggtctgct ttgtgcccac ctgccgataa tgcttaatta ggcgaggagt tgattaattt
     6901 gaggtaattg acagctaatt agaagcctac aggctcatta acttgacatc ttgtctttgt
     6961 gaattggctg ggtacggtca ggtgatggta accgttgctc ccctccccag ctcttccaac
     7021 cccaaagtct gaggcatcac ctaggggagg gctgggcaga tccggggttc aggtagggca
     7081 gtctcaagat caagagtaga gggaactggt actgaagagt aaggagtccc gaatggagac
     7141 gtctgctctg gctctaggga ggagagagaa caagaatgaa gggcaaatgg acccgagaga
     7201 gaactggacc ctagcaggag aatggggaag cccgaagctc acatagtgag aaggggctgg
     7261 ggagcaggtg agcctgttgg tactaggggt aggctttgaa gagaggccga cttgtggtta
     7321 aatcccagct ctgcctagag ctaggagact ccaactgacc taaatgctct ctaagcccgg
     7381 ggctcgtttg taaaacagga taacaatgtc taccttacct catttggtta ctgtgaacag
     7441 ttcatgggac acagaggcaa ggcctaggaa gcacccagtt aaatgtcact gactgggctg
     7501 gcaggatggg tcagtgggtg caaacttgat gacccaagct tgatccctgg aaccggggtg
     7561 gaaggagaga actgagtctg gaaggtccct cccacctgca cacttgcact gtggcaaaca
     7621 tcttcctgtg ctcacttaca catcacacac actcactctc tctcacacac acacacacac
     7681 aatgagtaaa acctaaagaa tgttcactat ccttggcagt gggaagacgt gactgggaga
     7741 tagtctaaga gggcaggttg tcagaggcaa tgggaagccc ctcccttgga gaactagtgt
     7801 cactgtggac atttgcattc acagcacaag agtgtttgat ggtgtgggga tcaggtcttc
     7861 ttgtcgcaac ctctttaggg ggcaatttag tgatctatat tacaatgtat atgtagcttt
     7921 tgacccaaga gttccctgct attaacgcac tctgaggatt tacatgtatg cgcaaggatt
     7981 tcattacagc gcagcctgca gaaccttcct gcgtatccca gttggaaaga ctggaaatca
     8041 cccaaaagtc catcagcgac taagttatgg tacctccccg tagtacagta tattactatt
     8101 gaatatatat gagcaggatc caaaatgcat taaatagggg gaaaagccac gtatataaca
     8161 gtgtatagtt cccacaagta gctcagtgga tggaggtaca ggaaatggga ggcgtatttt
     8221 tggcttgtac ttatcacctt ttcaaaaatc agtttacaat taaccccccc accccccata
     8281 agccctaaca atactgctat aatggcagag tagcgccatg aacaagggcg ggaacacttg
     8341 gtttcttctt ctacacagac aaacatggtg gccattggcc acgtgtggcc attgaaccct
     8401 tgaaatgtgg tctttttttt tttttttttt ttttaagagg atggagaggt ggttcagtgg
     8461 ttagaagtac gtactgctct tgtgaatttg gttctttttt ttatttattt atttagtcga
     8521 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8641 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8701 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8761 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8821 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8881 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8941 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9001 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9061 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9121 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9181 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9241 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9301 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9361 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9421 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9481 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9541 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9601 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9661 cgtagaaaag atcaaaggat cttc
//
