LOCUS       Exported                7073 bp ds-DNA     circular SYN 22-JUL-2024
DEFINITION  mR0-37a.
ACCESSION   mR0-37a
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..7073
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..826
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    complement(828..962)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     rep_origin      complement(982..1117)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(1141..1163)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             1170..1209
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1258..1288
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1296..1312
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(1697..1752)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1824..1895)
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     CDS             complement(2313..3813)
                     /label=SEAP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
     misc_feature    complement(3826..4288)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4398..4418
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=95.2"
                     /note="/match_length=8.2"
                     /note="/other=promoter"
     CDS             complement(4431..5150)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     regulatory      5147..5156
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    complement(5158..5184)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=34.6"
                     /note="/other=misc_feature"
     promoter        complement(5191..5387)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      5205..5340
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5404..5904
                     /label=RefinedRegion0
     promoter        5938..6042
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             6043..6903
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagattc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     1861 accaccttct gataggcagc ctgcacctga ggagtgcggc cggccgcgat atcctcgacc
     1921 ccactgggct gtaggtggtg gctggatggc ctgagccccc tcctttcctg gggtgcaggc
     1981 agccacaggg cacaacctgc agtccctggg gttggggtag ggagtccgag agcagccaag
     2041 gggcaggttt ggtgtcatgg gctcagtccc cagagcgcac ggggagggga aggttccatg
     2101 gcaggacctc tgtataggga ctccggggat gacgactcgg gctggaggcc aggcaggacg
     2161 acaggaggtg gggagcagga gaactccggg atggggaagc aggagcccca gggacgggac
     2221 actcagggag cagtggccgt ctccagcagc agcagggtcc cggccagcag aggaagcaac
     2281 gcggggacca cggaccgccc cgggtgcgcg gcgtcggtgg tgccggcggg gggcgccagg
     2341 tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc tatgaaggtc
     2401 tgctcctgca cgccgtgaac caggtgcgcc tgcgggccgc gcgcgaacac cgccacgtcc
     2461 tcgcctgcgt gggtctcttc gtccaggggc actgctgact gctgccgata ctcggggctc
     2521 ccgctctcgc tctcggtaac atccggccgg gcgccgtcct tgagcacata gcctggaccg
     2581 tttccgtata ggaggaccgt gtaggccttc ctgtcccggg ccttgccagg ggccagcccg
     2641 aagatggagc tccctcgcag ggggtagcct ccgaaggaga agacgtggga gtggtcggca
     2701 gtgacgaggc tcagcgtgtc ctcctcgctg gtgagctggc ccgccctctc aatggcgtcg
     2761 tcgaacatga tcgtctcagt cagtgcccgg taagccctgc tttcatgatg accatggtcg
     2821 atgcgaccac cctccacgaa gaggaagaag ccgcgggggt tcctgctcag caggcgcagg
     2881 gcagcctctg tcatctccat cagggagggg tccagtgtgg agtctcggtg gatctcgtat
     2941 ttcatgtctc caggctcaaa gagacccatg agatgggtca cagacgggtc cagggaagcc
     3001 tgcatgagct cagtgcggtt ccacacatac cgggcaccct ggcgcttcgc cagccattcc
     3061 tgcaccagat tcttcccgtc cagcctggtc ccaccttggc tgtagtcatc tgggtactca
     3121 gggtctgggg ttcccatgcg aaacatgtac tttcggcctc cacctaggat cacgtcaatg
     3181 tccatgttgg agatgagctg cgtagcgatg tcctggcacc cctcctggcg ggccgaggca
     3241 ggcacgtcgg cgtccgagta ccagttgcgg ttcaccgtgt gggcgtaggt gccggctggc
     3301 gaggcgtgct gcactcgtgt ggtggttacc actcccactg acttccctgc tttcttggcc
     3361 cgattcatca cggagatgac ctcgttgccg cgtgtcgtgt tgcactggtt aaagcgggcg
     3421 gctgcactca agccaatggt ctggaagttg cccttgaccc cgcacaggta ggccgtggct
     3481 gtggctccac tgtctggcac atgtttgtct acattgtatg tcttggacag agccacatat
     3541 gggaagcggt ccatggccag gggtatctca ggccccagtt tgtccttctt ctgccctttt
     3601 aggatcctgg cagctgtcac cgtagacacc cccatcccat cgcccaggaa gatgatgagg
     3661 ttcttggcgg ctgtctgtgc aggctgcagc ttcttggcgg cacccagggc ctcggctgcc
     3721 tcgcggttcc agaagtccgg gttctcctcc tcaactggga tgatgcccag ggagagctgt
     3781 agcctcaggc ccagcagcag cagcagcagc agcctcgacc ccatattatc atcgtgtttt
     3841 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     3901 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     3961 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     4021 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     4081 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     4141 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     4201 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     4261 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     4321 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     4381 gggggggggg agggagaggg gggggggggg ggggggcgga attggccgct ttacttgtac
     4441 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     4501 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     4561 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     4621 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     4681 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     4741 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     4801 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     4861 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     4921 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     4981 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     5041 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     5101 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc
     5161 ggtggatccc gggcccgcgg taccaagctt tttgcaaaag cctaggcctc caaaaaagcc
     5221 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     5281 aaaaattagt cagcgatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     5341 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcagat cgcagatctc
     5401 gaggaccgtg ttccccatcc ctcctccctg catctgtccc ctggttagag ttgtatgaag
     5461 cggaagacaa tctggggctt attagtgtcc cagggagaca tgtaactgtg ctgctgggac
     5521 ccaaacagca agagtcttag cagcaaactc cagccgagaa aggactgagg gattgtctca
     5581 cctaattaca gggaatggct ctctttgtat ttgtgattcg gtacggttgt cagatcgctt
     5641 ttgatcgctt ttctccattt gctggctcag gccatgctgg aattgtgcta attagaggag
     5701 aaatggacag agtgatcctg tcaaccctgg ttactggtgg gggacaaagc agaatattat
     5761 tctaaggcaa tgttttgtaa gagcccttct cctgctctca ttaggaggaa gaggccccag
     5821 gaggggaagt tccgaatgct tggcctggct aatggagcat tggggtgttc ttgtgctaat
     5881 taacaggctc tgccagcact ccaggtcgac ccaggtggca cttttcgggg aaatgtgcgc
     5941 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     6001 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     6061 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     6121 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     6181 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     6241 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     6301 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     6361 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     6421 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     6481 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     6541 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     6601 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     6661 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     6721 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     6781 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     6841 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     6901 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     6961 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     7021 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
