LOCUS       Exported                6764 bp ds-DNA     circular SYN 22-JUL-2024
DEFINITION  mR3-17c.
ACCESSION   mR3-17c
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..6764
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..826
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    complement(828..962)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        complement(968..1163)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      complement(982..1117)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(1141..1163)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             1170..1209
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1258..1288
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1296..1312
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     1320..1336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(1697..1752)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1824..1895)
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     CDS             complement(2313..3813)
                     /label=SEAP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
     CDS             complement(2313..3812)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    3824..4376
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3826..4401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    complement(3826..4288)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4400..4418
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.4"
                     /note="/other=promoter"
     CDS             complement(4430..5149)
                     /label=EGFP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
     regulatory      5146..5155
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    complement(5157..5183)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=34.6"
                     /note="/other=misc_feature"
     promoter        complement(5190..5386)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      5204..5339
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5403..5595
                     /label=RefinedRegion3_A
     promoter        5629..5733
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             5734..6594
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagattc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     1861 accaccttct gataggcagc ctgcacctga ggagtgcggc cggccgcgat atcctcgacc
     1921 ccactgggct gtaggtggtg gctggatggc ctgagccccc tcctttcctg gggtgcaggc
     1981 agccacaggg cacaacctgc agtccctggg gttggggtag ggagtccgag agcagccaag
     2041 gggcaggttt ggtgtcatgg gctcagtccc cagagcgcac ggggagggga aggttccatg
     2101 gcaggacctc tgtataggga ctccggggat gacgactcgg gctggaggcc aggcaggacg
     2161 acaggaggtg gggagcagga gaactccggg atggggaagc aggagcccca gggacgggac
     2221 actcagggag cagtggccgt ctccagcagc agcagggtcc cggccagcag aggaagcaac
     2281 gcggggacca cggaccgccc cgggtgcgcg gcgtcggtgg tgccggcggg gggcgccagg
     2341 tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc tatgaaggtc
     2401 tgctcctgca cgccgtgaac caggtgcgcc tgcgggccgc gcgcgaacac cgccacgtcc
     2461 tcgcctgcgt gggtctcttc gtccaggggc actgctgact gctgccgata ctcggggctc
     2521 ccgctctcgc tctcggtaac atccggccgg gcgccgtcct tgagcacata gcctggaccg
     2581 tttccgtata ggaggaccgt gtaggccttc ctgtcccggg ccttgccagg ggccagcccg
     2641 aagatggagc tccctcgcag ggggtagcct ccgaaggaga agacgtggga gtggtcggca
     2701 gtgacgaggc tcagcgtgtc ctcctcgctg gtgagctggc ccgccctctc aatggcgtcg
     2761 tcgaacatga tcgtctcagt cagtgcccgg taagccctgc tttcatgatg accatggtcg
     2821 atgcgaccac cctccacgaa gaggaagaag ccgcgggggt tcctgctcag caggcgcagg
     2881 gcagcctctg tcatctccat cagggagggg tccagtgtgg agtctcggtg gatctcgtat
     2941 ttcatgtctc caggctcaaa gagacccatg agatgggtca cagacgggtc cagggaagcc
     3001 tgcatgagct cagtgcggtt ccacacatac cgggcaccct ggcgcttcgc cagccattcc
     3061 tgcaccagat tcttcccgtc cagcctggtc ccaccttggc tgtagtcatc tgggtactca
     3121 gggtctgggg ttcccatgcg aaacatgtac tttcggcctc cacctaggat cacgtcaatg
     3181 tccatgttgg agatgagctg cgtagcgatg tcctggcacc cctcctggcg ggccgaggca
     3241 ggcacgtcgg cgtccgagta ccagttgcgg ttcaccgtgt gggcgtaggt gccggctggc
     3301 gaggcgtgct gcactcgtgt ggtggttacc actcccactg acttccctgc tttcttggcc
     3361 cgattcatca cggagatgac ctcgttgccg cgtgtcgtgt tgcactggtt aaagcgggcg
     3421 gctgcactca agccaatggt ctggaagttg cccttgaccc cgcacaggta ggccgtggct
     3481 gtggctccac tgtctggcac atgtttgtct acattgtatg tcttggacag agccacatat
     3541 gggaagcggt ccatggccag gggtatctca ggccccagtt tgtccttctt ctgccctttt
     3601 aggatcctgg cagctgtcac cgtagacacc cccatcccat cgcccaggaa gatgatgagg
     3661 ttcttggcgg ctgtctgtgc aggctgcagc ttcttggcgg cacccagggc ctcggctgcc
     3721 tcgcggttcc agaagtccgg gttctcctcc tcaactggga tgatgcccag ggagagctgt
     3781 agcctcaggc ccagcagcag cagcagcagc agcctcgacc ccatattatc atcgtgtttt
     3841 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     3901 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     3961 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     4021 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     4081 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     4141 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     4201 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     4261 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     4321 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     4381 gggggggggg agggagaggg gggggggggg gggggcggaa ttggccgctt tacttgtaca
     4441 gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg accatgtgat
     4501 cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag tggttgtcgg
     4561 gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg gcgagctgca
     4621 cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg ttcttctgct
     4681 tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg tgccccagga
     4741 tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc agggtgtcgc
     4801 cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag aagatggtgc
     4861 gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc ttcatgtggt
     4921 cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg gtgggccagg
     4981 gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg taggtggcat
     5041 cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg tccagctcga
     5101 ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg gtggcgaccg
     5161 gtggatcccg ggcccgcggt accaagcttt ttgcaaaagc ctaggcctcc aaaaaagcct
     5221 cctcactact tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa
     5281 aaaattagtc agcgatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg
     5341 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcagatc gcagatctcg
     5401 aggtttcttt ctcttgtccc tccttctgta atctgacctc atcaagccct gggaaggctg
     5461 atccaccgga gcaaatcctt catccagagg gccaaaatca gcttaatttg tagcaatttg
     5521 aagcaggtct ttgccaggat ttgaatatca aattatccca tagcctagac aggcctggga
     5581 gcttccccta cccccgtcga cccaggtggc acttttcggg gaaatgtgcg cggaacccct
     5641 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     5701 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5761 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5821 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5881 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5941 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     6001 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     6061 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     6121 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     6181 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     6241 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     6301 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     6361 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     6421 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     6481 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     6541 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     6601 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     6661 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6721 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
