LOCUS       Exported               14709 bp ds-DNA     circular SYN 22-JUL-2024
DEFINITION  mR0+R1+R3.
ACCESSION   mR0+R1+R3
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 16, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     source          1..14709
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             772..826
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    complement(828..962)
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     promoter        complement(968..1163)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      complement(982..1117)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        complement(1141..1163)
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     CDS             1170..1209
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    1222..1243
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1258..1288
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1292..1314
                     /label=lac operator (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=92.0"
                     /note="/other=protein_bind"
     protein_bind    1296..1312
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1320..1336
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    complement(1697..1752)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(1824..1895)
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     CDS             complement(2313..3813)
                     /label=SEAP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
     CDS             complement(2313..3812)
                     /codon_start=1
                     /product="secreted alkaline phosphatase from human 
                     placenta"
                     /label=SEAP
                     /note="The natural enzyme has a C-terminal transmembrane 
                     sequence that is replaced by a GPI anchor."
                     /translation="LLLLLLLGLRLQLSLGIIPVEEENPDFWNREAAEALGAAKKLQPA
                     QTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKH
                     VPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNRAKKAGKSVGVVT
                     TTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDIDVILGGGRKY
                     MFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHL
                     MGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRA
                     YRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKAR
                     DRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEETHAGEDVAVFAR
                     GPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD"
     misc_feature    3824..4376
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    3826..4401
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    complement(3826..4288)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     promoter        4398..4416
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.4"
                     /note="/other=promoter"
     CDS             complement(4428..5148)
                     /label=EGFP (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=99.9"
                     /note="/match_length=100.1"
                     /note="/other=CDS"
     regulatory      5145..5154
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    complement(5156..5182)
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=34.6"
                     /note="/other=misc_feature"
     promoter        complement(5189..5385)
                     /label=SV40 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.5"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     rep_origin      5203..5338
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     misc_feature    5402..9454
                     /label=mRegion3
     misc_feature    7027..7678
                     /label=RefinedRegion3_B
     promoter        complement(8139..8157)
                     /label=chicken beta-actin promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.4"
                     /note="/other=promoter"
     misc_feature    8159..8351
                     /label=RefinedRegion3_A
     misc_feature    9455..12575
                     /label=mRegion1
     misc_feature    10836..11117
                     /label=RefinedRegion1
     misc_feature    complement(12490..12507)
                     /label=HA-L (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.6"
                     /note="/other=misc_feature"
     misc_feature    12576..13540
                     /label=mRegion0
     misc_feature    12865..13365
                     /label=RefinedRegion0
     promoter        13574..13678
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             13679..14539
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga
      841 taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta
      901 tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag
      961 ttagcttttt gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     1021 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     1081 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta
     1141 tggttgctga ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc
     1201 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     1261 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     1321 aggaaacagc tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata
     1381 agagaagagg gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta
     1441 aaacatgtaa ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatataa
     1501 aaaaaatcta acctcaagtc aaggcttttc tatggaataa ggaatggaca gcagggggct
     1561 gtttcatata ctgatgacct ctttatagcc aacctttgtt catggcagcc agcatatggg
     1621 catatgttgc caaactctaa accaaatact cattctgatg ttttaaatga tttgccctcc
     1681 catatgtcct tccgagtgag agacacaaaa aattccaaca cactattgca atgaaaataa
     1741 atttccttta ttagccagaa gtcagatgct caaggggctt catgatgtcc ccataatttt
     1801 tggcagaggg aaaaagattc tcagtggtat ttgtgagcca gggcattggc cacaccagcc
     1861 accaccttct gataggcagc ctgcacctga ggagtgcggc cggccgcgat atcctcgacc
     1921 ccactgggct gtaggtggtg gctggatggc ctgagccccc tcctttcctg gggtgcaggc
     1981 agccacaggg cacaacctgc agtccctggg gttggggtag ggagtccgag agcagccaag
     2041 gggcaggttt ggtgtcatgg gctcagtccc cagagcgcac ggggagggga aggttccatg
     2101 gcaggacctc tgtataggga ctccggggat gacgactcgg gctggaggcc aggcaggacg
     2161 acaggaggtg gggagcagga gaactccggg atggggaagc aggagcccca gggacgggac
     2221 actcagggag cagtggccgt ctccagcagc agcagggtcc cggccagcag aggaagcaac
     2281 gcggggacca cggaccgccc cgggtgcgcg gcgtcggtgg tgccggcggg gggcgccagg
     2341 tcgcaggcgg tgtagggctc caggcaggcg gcgaaggcca tgacgtgcgc tatgaaggtc
     2401 tgctcctgca cgccgtgaac caggtgcgcc tgcgggccgc gcgcgaacac cgccacgtcc
     2461 tcgcctgcgt gggtctcttc gtccaggggc actgctgact gctgccgata ctcggggctc
     2521 ccgctctcgc tctcggtaac atccggccgg gcgccgtcct tgagcacata gcctggaccg
     2581 tttccgtata ggaggaccgt gtaggccttc ctgtcccggg ccttgccagg ggccagcccg
     2641 aagatggagc tccctcgcag ggggtagcct ccgaaggaga agacgtggga gtggtcggca
     2701 gtgacgaggc tcagcgtgtc ctcctcgctg gtgagctggc ccgccctctc aatggcgtcg
     2761 tcgaacatga tcgtctcagt cagtgcccgg taagccctgc tttcatgatg accatggtcg
     2821 atgcgaccac cctccacgaa gaggaagaag ccgcgggggt tcctgctcag caggcgcagg
     2881 gcagcctctg tcatctccat cagggagggg tccagtgtgg agtctcggtg gatctcgtat
     2941 ttcatgtctc caggctcaaa gagacccatg agatgggtca cagacgggtc cagggaagcc
     3001 tgcatgagct cagtgcggtt ccacacatac cgggcaccct ggcgcttcgc cagccattcc
     3061 tgcaccagat tcttcccgtc cagcctggtc ccaccttggc tgtagtcatc tgggtactca
     3121 gggtctgggg ttcccatgcg aaacatgtac tttcggcctc cacctaggat cacgtcaatg
     3181 tccatgttgg agatgagctg cgtagcgatg tcctggcacc cctcctggcg ggccgaggca
     3241 ggcacgtcgg cgtccgagta ccagttgcgg ttcaccgtgt gggcgtaggt gccggctggc
     3301 gaggcgtgct gcactcgtgt ggtggttacc actcccactg acttccctgc tttcttggcc
     3361 cgattcatca cggagatgac ctcgttgccg cgtgtcgtgt tgcactggtt aaagcgggcg
     3421 gctgcactca agccaatggt ctggaagttg cccttgaccc cgcacaggta ggccgtggct
     3481 gtggctccac tgtctggcac atgtttgtct acattgtatg tcttggacag agccacatat
     3541 gggaagcggt ccatggccag gggtatctca ggccccagtt tgtccttctt ctgccctttt
     3601 aggatcctgg cagctgtcac cgtagacacc cccatcccat cgcccaggaa gatgatgagg
     3661 ttcttggcgg ctgtctgtgc aggctgcagc ttcttggcgg cacccagggc ctcggctgcc
     3721 tcgcggttcc agaagtccgg gttctcctcc tcaactggga tgatgcccag ggagagctgt
     3781 agcctcaggc ccagcagcag cagcagcagc agcctcgacc ccatattatc atcgtgtttt
     3841 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta
     3901 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt
     3961 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac
     4021 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac
     4081 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag
     4141 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac
     4201 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa
     4261 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa
     4321 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg
     4381 gggggggggg agggagaggg gggggggggg gggcggaatt ggccgcttta cttgtacagc
     4441 tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg
     4501 cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc
     4561 agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg
     4621 ctgccgtcct cgatgttgtg gcggattctt gaagttcacc ttgatgccgt tcttctgctt
     4681 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
     4741 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
     4801 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
     4861 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
     4921 ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg
     4981 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
     5041 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
     5101 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg
     5161 tggatcccgg gcccgcggta ccaagctttt tgcaaaagcc taggcctcca aaaaagcctc
     5221 ctcactactt ctggaatagc tcagaggccg aggcggcctc ggcctctgca taaataaaaa
     5281 aaattagtca gcgatggggc ggagaatggg cggaactggg cggagttagg ggcgggatgg
     5341 gcggagttag gggcgggact atggttgctg actaattgag atgcagatcg cagatctcga
     5401 gagacatacg gggaagcctt cctgagtggg gtagtaacag gactggactc tgaaggactg
     5461 gcttacaggg acttcctcat atttgacctg aagcaatgac accttcaacc gctttaacct
     5521 ataaccacct cttctcagtg cttttcccct gaacaaaagg aaaaaaaaac atcttctact
     5581 cagcagacct cctcatggtt cccctcagcc atcctgaggt ggctgctagg ccctcaagag
     5641 ccgggtatct ccaccccatc tcaaaatttt ctcttggcct gaaatcaggg gccggagtaa
     5701 ctttcctgca atgctgaaga tgttgggcag agaggaaatt atgtccatgt ccctggggcc
     5761 ccaccccaca atgccccctg cagtgaggaa caaccatgag ggctgctcaa gggggaggtg
     5821 aatgaagaaa cacagctagg ggagggcttg gtctcacaat ccagttcccc ttaatatccc
     5881 ataataaggt gctgtcaaca tgagtttccc gttcaagtag atttgctaag aaggctgcgg
     5941 gaggtcagag gtgctttctg gccctcctgg tatggggcac aggggccctt cctcaccttt
     6001 tccccctctg cagtctaaat agactcattt gtcagcctgt gtgtacccgg gttccatgaa
     6061 tccgtgacgg ggtgttagag gcccttgagc cctctgtgtc tcctccgaga tacaaaaaca
     6121 ggattttgtt ttaatgtgag ggagctcaga gcttgaccag gaaggtaggc atggaggtcc
     6181 cagggagaca gggctccaca tgtagaggga tccagaatgc gttggccgac ctggtgttta
     6241 gattggctgc tggctactca ctctgctttc ccatggaatc cctgttaaac actccctctt
     6301 tccccctggg gtggggcaga gacctggctt ctcaggtgtc tctctgtaga atggtgtgag
     6361 ctcttgaaga gagtagtaaa ggagacatgg tcgccatgta ccaggccggc tctagagcgt
     6421 cccatatgga attgcttcct gcagttcggt ggagctggga caggcagaaa caccaagggg
     6481 tcaaggctgg ggatggaagc tgtgtaagaa caggctgtgg tacctatttc cccaagccag
     6541 caggggctgt tggaatttaa gaaagaagtc caggcatgat ggaacatgcc catacatccc
     6601 agcacttgcg gggctgaggc aggaggatca ccagtttgag gtcagtgtgg cctatacata
     6661 tatatcaaac aaaactagac cagtgtggcc tatacatata tatcaaacaa aactagacca
     6721 gtgtgtggaa cagaggagga caggaatgga gccccggtgg ggactgtggg gagaggcagg
     6781 aggagctggc tgtccggagg tcaaaggatc tagacatctg ggcctgggct cagatggggc
     6841 accttgtgca cctctgcctg gaagcatctg gagtggccga ggtgaacagg atgaagtcat
     6901 actgtgccct ccccagggga ccagtggtgg gtgccaatga gctatgtggt aagaccatgg
     6961 gggccatagg acaggaaggc aggttggggg ctgaggaggg cagagctggg tttctatccc
     7021 ttttgtgagc cgttttaatt cgtgtaaatg agggttttta atttgtcatt ctgggtttta
     7081 gttctattac cgtctcattt gcctctaaac cctatttcgc ctcagcctgg accgaattcc
     7141 atttgatgca ttagaactaa ttctcctcct aaggggttag acgttttctc cccacacaaa
     7201 agtcgcttat tttggataaa gagacgatta gatttatctt taatatttta atggcagctg
     7261 ggattagcgg gcaagcagcc ccagccagtg ctcttctccc cggattaggt tctgataaac
     7321 tctcagatgg ggagcccccc ccccccgtac tccactttct gttttctaca aagtgctttt
     7381 ttattatttt tctgtgggaa aaaaattccc ttgtcttttg gagcatcata aattccattt
     7441 gctgttttct attaaatcaa gttgattaaa ttcgttgtga ctctttgcac atagattctt
     7501 tttaatttta acgtctccag cataaaatct ttaatggtgc aatttaaaat tgctgcaata
     7561 tacagtgcag gccctgatca agcagatgga gatgaggttc tgccgggtgg gggggagtga
     7621 ggagaaggcc gggattgact cttccctgaa gaactgagtg tgaccctgag tacccacact
     7681 caagcgcctt ttcagccacg ctgtcgagct tcaggctttc cctagcaccc tggccagaac
     7741 ttacagacaa cttgcctgtg cttcttactg tgtgtgtcct gctaatactc cttataagaa
     7801 gggttggtgg ctgaaggtca cacagccatt gggtccaatt tgtgtggtct gcggccagcc
     7861 cccccacctc aactctcaga gcctctatct atttccttac cagtgaaatg gggtgattgg
     7921 ttctgcacgg actcccatgg cttcgataaa gacttgatac agactgcctt gtgaacagtg
     7981 cagggctggg ctttcctgga tatctggggg attcttccgg tcctatttgg gaccagacac
     8041 tctgtcatcc gtcccaggac cggtagagct gaattttgag gtcttgggaa ttttccctaa
     8101 ctaccaggtc ttggctgccc cctcccctta actcctagcc gccccccccc ccccccccgt
     8161 ttctttctct tgtccctcct tctgtaatct gacctcatca agccctggga aggctgatcc
     8221 accggagcaa atccttcatc cagagggcca aaatcagctt aatttgtagc aatttgaagc
     8281 aggtctttgc caggatttga atatcaaatt atcccatagc ctagacaggc ctgggagctt
     8341 cccctacccc catctcccca ctgcaatatg gatggatgtg ttctgagatc catgggggac
     8401 actgaagaga ccagactggg ccagaggatg ctgatggtat caggtgtgct gggggaaaat
     8461 ggcaccagtc agcccatccc aggcctgaga aggaggacag ggaggagaca ggaacttcct
     8521 gattgggaga ctgatcccct tgtcaaacct tcagggaaca tggacagatt tttcttgtca
     8581 tttaataata cagtataaag taatgataat ataagcatac agtttacacc atgctcacat
     8641 cgtcgtagtt atcactagag gagctgaagt ggcccgaggg acatacatga attacatgca
     8701 aatattctgc catctgtctg tttggttttt tttttttttc caagacaggg tttctctgtg
     8761 taaccctggt tatcctggac ctcactctgt agaccaggtt ggcctcagaa ctcacagaga
     8821 tcctcctgcc tctgcctcct gagtgttcag attaagttcc tgtgccacca ctgctcagct
     8881 caaaagacta ttttgatgac taggaaagtc tggaaaaatc tcagctggcc aatcaacaaa
     8941 caccttgccc acctctctcc agcccccggt gtctggggaa gatccgaccc tcagtgctac
     9001 aagccatttt ctttacagac tgatccatgc tggccctgct tgctcagact agccgctaca
     9061 cctctttggc ccttctggtt agtcctgttt cctctggcaa cctccctctt gattcctggt
     9121 gtgtcctggg cctggaagat gtccacaccc acccaccaca ggggatgcac tgcccagacc
     9181 tgagaaccgg agttgtacct gtctcatggg cctgtcatgc ctcgtgggat gtgaaagagt
     9241 cctttgtaag cctgtctgtc cacccaatag caagaggaga aaccatccag atgtggaggt
     9301 acataattta atcccagcgc ctgggaggca gagaagggag ggtttctgtg agttcaagga
     9361 cagcctggtc tacatattaa gctctaggct agccaggggt atacaatgag actttgtcaa
     9421 aaaaaaaaaa aaaaaaaaaa aaaaaaaaag ccggtggcat gcatgtatat gtgtactggc
     9481 atgcgtgtat atatgtactg gcatgtgtgt aaaccttgcg cttaggaggc tgaacaggag
     9541 gactgtgagt tcaaggacag cctagactat acactgagtc tctggctaaa caaatactgt
     9601 aaacacagca tctatcttga taaacaaccc attaaacata cctatggaca caaataatat
     9661 attcaccaat tttttttata tgctattcct gccagcacac agtaggcact tcctaagggc
     9721 ctactgtccc tttcatctcc agggcacaac attaacaatt ctgtttgccc agccacctcc
     9781 ctttccactg tgacccttga tcagcaagca caataaatgt ttgctaggtg actgatttat
     9841 accagggatg agggaacgtc tccaagcttc cacagaagtg aaagacaagt ggcctccatc
     9901 gccccacaag ggaagatgat gtcaggtgcc agggtggaga tggtgaacat actcggtgtg
     9961 ctggagtccc agcagccaag ctcaccctgt cccagggtca aaagtattca gctgccagga
    10021 ccatatcaga aatgtaatct cagtacccaa ggtctgtcct aaggcctggc tccttaggcc
    10081 ttgcacctcc cacctaagtt ggacagggcc ctttcttcca cttctttagt tggagatggg
    10141 tgtttaactc atatgatgag ccatgtattg tatcagggct ctttgtgagt ggagagggac
    10201 acctgagccc tgggtaccca aaggtgtaga gagatgggga cacagatgta gccagaatac
    10261 ctcagcctcc agcacctggc atgttgtaga gcaccaattc acagtcttca ggggtgtact
    10321 gaaagctggg gactgtcagg agcctagagg taccgactcc ataccctgag cagttcttag
    10381 gcgaagccgc tgagttgtgt gtggctcgcc tccactgtta caccactgaa cacactgact
    10441 agtgtggacc aggcacttcc caaggagagt ctcagaggtc tgggaaggcc ttgaaaaagt
    10501 cttctcaatt tttagaatgg aaaagcagcc caaagaagtc taagtcattt ccagtgccac
    10561 agagttcaca tccgctggga gcctcaactc ccggcctcct cttggctcca ctcctgccca
    10621 ggatctcagg ggtcaggggc tgctcttgag ggttcttcat ggctctccat tcctactgat
    10681 ccacttccct aaagtcccac cggactctga gctctacatc tccctgtcca ctcactcagt
    10741 tcctcaggag gtcctggaga ccccagtagt ggggagagag gtccttggtg cctcttagct
    10801 gtccgctagc tcagagctgt agggaggctg ttgcggtgca cagcactgat cctaaccaag
    10861 tttgccagcc cagcttttct catggctccc acacaatggt ctgctttgtg cccacctgcc
    10921 gataatgctt aattaggcga ggagttgatt aatttgaggt aattgacagc taattagaag
    10981 cctacaggct cattaacttg acatcttgtc tttgtgaatt ggctgggtac ggtcaggtga
    11041 tggtaaccgt tgctcccctc cccagctctt ccaaccccaa agtctgaggc atcacctagg
    11101 ggagggctgg gcagatccgg ggttcaggta gggcagtctc aagatcaaga gtagagggaa
    11161 ctggtactga agagtaagga gtcccgaatg gagacgtctg ctctggctct agggaggaga
    11221 gagaacaaga atgaagggca aatggacccg agagagaact ggaccctagc aggagaatgg
    11281 ggaagcccga agctcacata gtgagaaggg gctggggagc aggtgagcct gttggtacta
    11341 ggggtaggct ttgaagagag gccgacttgt ggttaaatcc cagctctgcc tagagctagg
    11401 agactccaac tgacctaaat gctctctaag cccggggctc gtttgtaaaa caggataaca
    11461 atgtctacct tacctcattt ggttactgtg aacagttcat gggacacaga ggcaaggcct
    11521 aggaagcacc cagttaaatg tcactgactg ggctggcagg atgggtcagt gggtgcaaac
    11581 ttgatgaccc aagcttgatc cctggaaccg gggtggaagg agagaactga gtctggaagg
    11641 tccctcccac ctgcacactt gcactgtggc aaacatcttc ctgtgctcac ttacacatca
    11701 cacacactca ctctctctca cacacacaca cacacaatga gtaaaaccta aagaatgttc
    11761 actatccttg gcagtgggaa gacgtgactg ggagatagtc taagagggca ggttgtcaga
    11821 ggcaatggga agcccctccc ttggagaact agtgtcactg tggacatttg cattcacagc
    11881 acaagagtgt ttgatggtgt ggggatcagg tcttcttgtc gcaacctctt tagggggcaa
    11941 tttagtgatc tatattacaa tgtatatgta gcttttgacc caagagttcc ctgctattaa
    12001 cgcactctga ggatttacat gtatgcgcaa ggatttcatt acagcgcagc ctgcagaacc
    12061 ttcctgcgta tcccagttgg aaagactgga aatcacccaa aagtccatca gcgactaagt
    12121 tatggtacct ccccgtagta cagtatatta ctattgaata tatatgagca ggatccaaaa
    12181 tgcattaaat agggggaaaa gccacgtata taacagtgta tagttcccac aagtagctca
    12241 gtggatggag gtacaggaaa tgggaggcgt atttttggct tgtacttatc accttttcaa
    12301 aaatcagttt acaattaacc cccccacccc ccataagccc taacaatact gctataatgg
    12361 cagagtagcg ccatgaacaa gggcgggaac acttggtttc ttcttctaca cagacaaaca
    12421 tggtggccat tggccacgtg tggccattga acccttgaaa tgtggtcttt tttttttttt
    12481 ttttttttta agaggatgga gaggtggttc agtggttaga agtacgtact gctcttgtga
    12541 atttggttct tttttttatt tatttattta tttatgtaaa ttctctttga catggtggta
    12601 agaacctcac tgcaggtctg ggtatggagg tctgggtgtc gtacctcagt caccgtggta
    12661 ccgcagtcac catgctgcag gccatgcaag gagtgagggc tggaggtaag gaaacagagc
    12721 gggcttccag gaaggtcaga gctccagtac ttcagactct ggctccaggg atggagccta
    12781 ggctgtgcca gtgcagttct gctctgctcc acagcctgtg ccttttgggc tttgggaaac
    12841 ctctcacgag ttgctttgtt tagagaccgt gttccccatc cctcctccct gcatctgtcc
    12901 cctggttaga gttgtatgaa gcggaagaca atctggggct tattagtgtc ccagggagac
    12961 atgtaactgt gctgctggga cccaaacagc aagagtctta gcagcaaact ccagccgaga
    13021 aaggactgag ggattgtctc acctaattac agggaatggc tctctttgta tttgtgattc
    13081 ggtacggttg tcagatcgct tttgatcgct tttctccatt tgctggctca ggccatgctg
    13141 gaattgtgct aattagagga gaaatggaca gagtgatcct gtcaaccctg gttactggtg
    13201 ggggacaaag cagaatatta ttctaaggca atgttttgta agagcccttc tcctgctctc
    13261 attaggagga agaggcccca ggaggggaag ttccgaatgc ttggcctggc taatggagca
    13321 ttggggtgtt cttgtgctaa ttaacaggct ctgccagcac tccagccaac caggggactc
    13381 gcagttgcac ttggtatctc ctgaagtctc caactgtccc tggtctaaaa caaaatctac
    13441 cagacaaaag gtgggaaggt caatggcctt ttcagacata ggggcacact tccaaagtgg
    13501 caaaggtcta aacagatatg aacagccagt tagacctagt gtcgacccag gtggcacttt
    13561 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    13621 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    13681 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    13741 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    13801 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    13861 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    13921 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    13981 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    14041 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    14101 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    14161 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    14221 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    14281 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    14341 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    14401 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    14461 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    14521 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    14581 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    14641 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    14701 aggatcttc
//
