LOCUS       pCSII-EKAR4602NL       12385 bp DNA     circular SYN 17-JUL-2024
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Wattlab
  TITLE     Direct Submission
  JOURNAL   Exported Aug 20, 2024 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12385
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        616..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     726..745
                     /label=CMVF_pCDNA3(A2)
                     /note="100"
     LTR             832..1012
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1056..1181
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1678..1911
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    2414..2531
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2587..3749
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     intron          2818..3740
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3662..3682
                     /label=pEF1a_fwd(A12)
                     /note="100"
     primer_bind     3681..3704
                     /label=pCSII-seq-Fw #6535/9313(A35)
                     /note="50	   c          A"
     primer_bind     3691..3714
                     /label=CSII-seq-F #5770/5868(A34)
                     /note="50	   c          A"
     primer_bind     3703..3724
                     /label=seq_PCSIIvector_f #8806(A33)
                     /note="50	   c          A"
     primer_bind     3721..3741
                     /label=EF-BOS-F #10232(A36)
                     /note="50	   c          A"
     CDS             3778..4488
                     /label=YPet_W
     misc_feature    4492..4656
                     /label=WW domain
                     /label=nonstandard type: WWdomain
     misc_feature    4658..5015
                     /label=EV linker
     CDS             5017..5070
                     /label=ERK substrate
     misc_feature    5032..5034
                     /label=p-site
     misc_feature    5080..5796
                     /label=mTurquioise_GL
     primer_bind     complement(5125..5146)
                     /label=EGFP_Nrev(A5)
                     /note="100"
     misc_feature    5803..5823
                     /direction=RIGHT
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /label=nonstandard type: NLS
                     /translation="PKKKRKV"
     misc_feature    6050..6636
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6145..6164)
                     /label=IRES-R(A53)
                     /note="100"
     primer_bind     6444..6463
                     /label=IRES-F(A55)
                     /note="100"
     CDS             6637..7035
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    7079..7667
                     /label=WPRE
                     /label=nonstandard type: WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     primer_bind     complement(7867..7886)
                     /label=3LTR-Rev(A20)
                     /note="100"
     primer_bind     complement(7867..7886)
                     /label=3LTR-Rev(A51)
                     /note="100"
     LTR             8186..8366
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8392..8409)
                     /label=BGH_Reverse(A1)
                     /note="100"
     polyA_signal    8398..8622
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8668..9096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        9110..9440
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9291..9426
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9330..9349
                     /label=SV40Fwd(A19)
                     /note="100"
     primer_bind     9330..9349
                     /label=SV40Fwd(A48)
                     /note="100"
     promoter        9488..9535
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9554..9928
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10058..10179
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     10095..10114
                     /label=SV40pA-Fwd(A73)
                     /note="100	( V [ N G   X p)"
     primer_bind     complement(10095..10114)
                     /label=SV40pA-R(A25)
                     /note="100"
     primer_bind     complement(10095..10114)
                     /label=SV40pA-R(A54)
                     /note="100"
     primer_bind     complement(10227..10244)
                     /label=M13R(A7)
                     /note="100"
     primer_bind     complement(10227..10244)
                     /label=M13R(A14)
                     /note="100"
     primer_bind     complement(10228..10244)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    10252..10268
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(10276..10306)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10321..10342
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(10630..11218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(11389..12249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin,
                     and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11439..11458)
                     /label=Amp-F(A23)
                     /note="100"
     primer_bind     12012..12031
                     /label=Amp-R(A24)
                     /note="100"
     promoter        complement(12250..12354)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc
      841 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      901 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      961 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1021 cgaacaggga cctgaaagcg aaagggaaac cagagctctc tcgacgcagg actcggcttg
     1081 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     1141 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     1201 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     1261 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     1321 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     1381 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     1441 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     1501 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     1561 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1621 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1681 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cctcaatgac
     1741 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1801 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1861 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1921 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1981 taaatctctg gaacagatct ggaatcacac gacctggatg gagtgggaca gagaaattaa
     2041 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     2101 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     2161 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     2221 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     2281 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     2341 aggtggagag agagacagag acagatccat tcgattagtg aacggatcta caaatggcag
     2401 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     2461 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     2521 ttcaaaattt tcgggtttat tacagggaca gcagaaattc actttgaatt aattcaagct
     2581 tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     2641 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     2701 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     2761 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     2821 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     2881 tgaattactt ccacctggct ccagtacgtg attcttgatc ccgagctgga gccaggggcg
     2941 ggccttgcgc tttaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
     3001 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
     3061 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
     3121 ttgtaaatgc gggccaggat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
     3181 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
     3241 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
     3301 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
     3361 cggaaagatg gccgcttccc ggccctgctc cagggggctc aaaatggagg acgcggcgct
     3421 cgggagagcg ggcgggtgag tcacccacac aaaggaaagg ggcctttccg tcctcagccg
     3481 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctgga
     3541 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     3601 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg
     3661 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa
     3721 gtttttttct tccatttcag gtgtcgtgaa cacgctaccg gtctcgacga attcggcatg
     3781 tctaaaggtg aagaattatt cactggtgtt gtcccaattt tggttgaatt agatggtgat
     3841 gttaatggtc acaaattttc tgtctccggt gaaggtgaag gtgatgctac gtacggtaaa
     3901 ttgaccttaa aattactctg tactactggt aaattgccag ttccatggcc aaccttagtc
     3961 actactttag gttatggtgt tcaatgtttt gctagatacc cagatcatat gaaacaacat
     4021 gactttttca agtctgccat gccagaaggt tatgttcaag aaagaactat ttttttcaaa
     4081 gatgacggta actacaagac cagagctgaa gtcaagtttg aaggtgatac cttagttaat
     4141 agaatcgaat taaaaggtat tgattttaaa gaagatggta acattttagg tcacaaattg
     4201 gaatacaact ataactctca caatgtttac atcactgctg acaaacaaaa gaatggtatc
     4261 aaagctaact tcaaaattag acacaacatt gaagatggtg gtgttcaatt agctgaccat
     4321 tatcaacaaa atactccaat tggtgatggt ccagtcttgt taccagacaa ccattactta
     4381 tcctatcaat ctgccttatt caaagatcca aacgaaaaga gagaccacat ggtcttgtta
     4441 gaatttttga ctgctgctgg tattaccgag ggtatgaatg aattgtacct cgagatggcg
     4501 gacgaggaga agctgccgcc cggctgggag aagcgcatga gccgcagctc aggccgagtg
     4561 tactacttca accacatcac taacgccagc cagtgggagc ggcccagcgg caacagcagc
     4621 agtggtggca aaaacgggca gggggagcct gccaggggta ccagtgctgg tggtagtgct
     4681 ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gttccggcag tgctggtggt
     4741 agtgctggtg gtagtaccag tgctggtggt agtgctggtg gtagtgctgg tggtagtgct
     4801 ggtggtagtg ctggtggttc cggcagtgct ggtggtagtg ctggtggtag taccagtgct
     4861 ggtggtagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggttccggc
     4921 agtgctggtg gtagtgctgg tggtagtacc agtgctggtg gtagtgctgg tggtagtgct
     4981 ggtggtagtg ctggtggtag tgctggtggt tccggaccag atgtccctag aactccagtg
     5041 gatccagcat ggctgtcatt ccaatttccg ggcggccgca tggtgagcaa gggcgaggag
     5101 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     5161 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     5221 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgtcctgg
     5281 ggcgtgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     5341 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     5401 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     5461 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc
     5521 agcgggaacg tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag
     5581 atccgccaca acatcgagga cggcggcgtg cagctcgccg accactacca gcagaacacc
     5641 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     5701 ttaagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cttgaccgcc
     5761 gccgggatca ctctcggcat ggacgagctg tctagatcta gacctaagaa gaagcgtaag
     5821 gtgtaagtcg acggtcgagt cgagaagcta gcctagtgcc atttgttcag tggttcgtag
     5881 ggctttcccc cactgtttgg ctttcagtta tatggatgat gtggtattgg gggccaagtc
     5941 tgtacagcat cttgagtccc tttttaccgc tgttaccaat tttcttttgt ctttgggtaa
     6001 acccgggaat tcggcctcga gggcggcggc cgcggctcta gaggatccgc ccctctccct
     6061 cccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt gcgtttgtct
     6121 atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc
     6181 ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga atgcaaggtc
     6241 tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa acaacgtctg
     6301 tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc tgcggccaaa
     6361 agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt
     6421 ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag gggctgaagg
     6481 atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtgc acatgcttta
     6541 catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg gacgtggttt
     6601 tcctttgaaa aacacgatga taatatggcc acaaccatgg ccaagccttt gtctcaagaa
     6661 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
     6721 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
     6781 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
     6841 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
     6901 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
     6961 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
     7021 gtgtgggagg gctaagtcga gcctctagag ttaacatcga gggatcaagc ttatcgataa
     7081 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     7141 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     7201 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     7261 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     7321 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     7381 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     7441 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     7501 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     7561 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     7621 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     7681 agacctggaa aaacatggag caatcacaag tagcaacaca gcagctacca atgctgcttg
     7741 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
     7801 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     7861 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     7921 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     7981 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     8041 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     8101 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     8161 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     8221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     8281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     8341 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
     8401 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     8461 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     8521 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     8581 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     8641 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     8701 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8761 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8821 ggggcatccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8881 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8941 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     9001 ctatctcggt ctattctttt gatttataag ggattttggg gatttcggcc tattggttaa
     9061 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     9121 agggtgtgga aagtccccag gctccccagg caggcagaag tatgcaaagc atgcatctca
     9181 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     9241 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     9301 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     9361 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     9421 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     9481 agcacgtgtt gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt
     9541 gaggaactaa accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt
     9601 cgccggagcg gtcgagttct ggaccgaccg gctcgggttc tcccgggact tcgtggagga
     9661 cgacttcgcc ggtgtggtcc gggacgacgt gaccctgttc atcagcgcgg tccaggacca
     9721 ggtggtgccg gacaacaccc tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc
     9781 cgagtggtcg gaggtcgtgt ccacgaactt ccgggacgcc tccgggccgg ccatgaccga
     9841 gatcggcgag cagccgtggg ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt
     9901 gcacttcgtg gccgaggagc aggactgaca cgtgctacga gatttcgatt ccaccgccgc
     9961 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
    10021 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
    10081 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
    10141 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
    10201 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    10261 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
    10321 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    10381 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    10441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    10501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    10561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    10621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    10681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    10741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    10801 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
    10861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    10921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    10981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    11041 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    11101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    11161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    11221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    11281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    11341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    11401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    11461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    11521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    11581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    11641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    11701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    11761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    11821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    11881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    11941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    12001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    12061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    12121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    12181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    12241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    12301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    12361 tccccgaaaa gtgccacctg acgtc
//
