LOCUS       pGENT_R2_GW_R1_R       14976 bp DNA     circular SYN 14-SEP-2020
FEATURES             Location/Qualifiers
     source          1..14976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    115..139
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      226..451
                     /label=T35S
     misc_recomb     461..585
                     /label=attR2
     CDS             complement(626..931)
                     /codon_start=1
                     /label=ccdB
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             complement(1273..1932)
                     /codon_start=1
                     /label=CmR(Chloramphenical)
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     misc_recomb     2041..2165
                     /label=attR1
     misc_feature    2173..2177
                     /label=Toreplace
     CDS             2178..6128
                     /codon_start=1
                     /label=RUBY_Casette
                     /translation="MDHATLAMILAIWFISFHFIKLLFSQQTTKLLPPGPKPLPIIGNI
                     LEVGKKPHRSFANLAKIHGPLISLRLGSVTTIVVSSADVAKEMFLKKDHPLSNRTIPNS
                     VTAGDHHKLTMSWLPVSPKWRNFRKITAVHLLSPQRLDACQTFRHAKVQQLYEYVQECA
                     QKGQAVDIGKAAFTTSLNLLSKLFFSVELAHHKSHTSQEFKELIWNIMEDIGKPNYADY
                     FPILGCVDPSGIRRRLACSFDKLIAVFQGIICERLAPDSSTTTTTTTDDVLDVLLQLFK
                     QNELTMGEINHLLVDIFDAGTDTTSSTFEWVMTELIRNPEMMEKAQEEIKQVLGKDKQI
                     QESDIINLPYLQAIIKETLRLHPPTVFLLPRKADTDVELYGYIVPKDAQILVNLWAIGR
                     DPNAWQNADIFSPERFIGCEIDVKGRDFGLLPFGAGRRICPGMNLAIRMLTLMLATLLQ
                     FFNWKLEGDISPKDLDMDEKFGIALQKTKPLKLIPIPRYGSGATNFSLLKQAGDVEENP
                     GPMKMMNGEDANDQMIKESFFITHGNPILTVEDTHPLRPFFETWREKIFSKKPKAILII
                     SGHWETVKPTVNAVHINDTIHDFDDYPAAMYQFKYPAPGEPELARKVEEILKKSGFETA
                     ETDQKRGLDHGAWVPLMLMYPEADIPVCQLSVQPHLDGTYHYNLGRALAPLKNDGVLII
                     GSGSATHPLDETPHYFDGVAPWAAAFDSWLRKALINGRFEEVNIYESKAPNWKLAHPFP
                     EHFYPLHVVLGAAGEKWKAELIHSSWDHGTLCHGSYKFTSAGSGATNFSLLKQAGDVEE
                     NPGPMTAIKMNTNGEGETQHILMIPFMAQGHLRPFLELAMFLYKRSHVIITLLTTPLNA
                     GFLRHLLHHHSYSSSGIRIVELPFNSTNHGLPPGIENTDKLTLPLVVSLFHSTISLDPH
                     LRDYISRHFSPARPPLCVIHDVFLGWVDQVAKDVGSTGVVFTTGGAYGTSAYVSIWNDL
                     PHQNYSDDQEFPLPGFPENHKFRRSQLHRFLRYADGSDDWSKYFQPQLRQSMKSFGWLC
                     NSVEEIETLGFSILRNYTKLPIWGIGPLIASPVQHSSSDNNSTGAEFVQWLSLKEPDSV
                     LYISFGSQNTISPTQMMELAAGLESSEKPFLWVIRAPFGFDINEEMRPEWLPEGFEERM
                     KVKKQGKLVYKLGPQLEILNHESIGGFLTHCGWNSILESLREGVPMLGWPLAAEQAYNL
                     KYLEDEMGVAVELARGLEGEISKEKVKRIVEMILERNEGSKGWEMKNRAVEMGKKLKDA
                     VNEEKELKGSSVKAIDDFLDAVMQAKLEPSLQ"
     CDS             3678..3734
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4569..4625
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6717..6741
                     /label=PZP222_homology
     primer_bind     complement(6725..6741)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        6970..7648
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     promoter        7594..7640
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     CDS             7712..8245
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MVRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     polyA_signal    8302..8476
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     polyA_signal    8302..8369
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     polyA_signal    8370..8471
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    8554..8578
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             9106..9897
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      10141..10729
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    10915..11055
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      11399..11593
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11659..12732)
                     /codon_start=1
                     /product="replication protein from Pseudomonas plasmid 
                     pVS1"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(13161..13790)
                     /codon_start=1
                     /product="stability protein from Pseudomonas plasmid pVS1"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgattatt ctaataaacg ctcttttctc ttaggtttac
      121 ccgccaatat atcctgtcaa acactgatag tttaaactga aggcgggaaa cgacaatctg
      181 atccaagctc aagctgctct agagggcccg acgtcgcatg cctgcaggtc actggatttt
      241 ggttttagga attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa
      301 gggtttctta tatgctcaac acatgagcga aaccctataa gaaccctaat tcccttatct
      361 gggaactact cacacattat tctggagaaa aatagagaga gatagatttg tagagagaga
      421 ctggtgattt ttgcggactc tagcatggcc gcgggatatc accactttgt acaagaaagc
      481 tgaacgagaa acgtaaaatg atataaatat caatatatta aattagattt tgcataaaaa
      541 acagactaca taatactgta aaacacaaca tatccagtca ctatggtcga cctgcagact
      601 ggctgtgtat aagggagcct gacatttata ttccccagaa catcaggtta atggcgtttt
      661 tgatgtcatt ttcgcggtgg ctgagatcag ccacttcttc cccgataacg gagaccggca
      721 cactggccat atcggtggtc atcatgcgcc agctttcatc cccgatatgc accaccgggt
      781 aaagttcacg ggagacttta tctgacagca gacgtgcact ggccaggggg atcaccatcc
      841 gtcgcccggg cgtgtcaata atatcactct gtacatccac aaacagacga taacggctct
      901 ctcttttata ggtgtaaacc ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa
      961 gagccgttca tttcaataaa ccgggcgacc tcagccatcc cttcctgatt ttccgctttc
     1021 cagcgttcgg cacgcagacg acgggcttca ttctgcatgg ttgtgcttac cagaccggag
     1081 atattgacat catatatgcc ttgagcaact gatagctgtc gctgtcaact gtcactgtaa
     1141 tacgctgctt catagcatac ctctttttga catacttcgg gtatacatat cagtatatat
     1201 tcttataccg caaaaatcag cgcgcaaata cgcatactgt tatctggctt ttagtaagcc
     1261 ggatccacgc gtttacgccc cgccctgcca ctcatcgcag tactgttgta attcattaag
     1321 cattctgccg acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat
     1381 cagcaccttg tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt
     1441 gtccatattg gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgagac
     1501 gaaaaacata ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc
     1561 cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag
     1621 cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca
     1681 tatcaccagc tcaccgtctt tcattgccat acggaattcc ggatgagcat tcatcaggcg
     1741 ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa
     1801 aaaggccgta atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa
     1861 tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat
     1921 ttttttctcc attttagctt ccttagctcc tgaaaatctc gacggatcct aactcaaaat
     1981 ccacacatta tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgcggccgc
     2041 catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
     2101 taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt tttgtacaaa
     2161 cttgtgatat cactagtatg gatcatgcga ccctcgccat gatcctcgcg atctggttca
     2221 tcagcttcca cttcatcaag ctgctgttct cccagcagac caccaagctg cttccgccag
     2281 gaccaaagcc gcttccgatc atcggcaaca tccttgaggt gggcaagaag ccgcatcggt
     2341 ccttcgccaa cctcgccaag attcacggcc cactcatttc cctcagactc ggctctgtga
     2401 ccaccatcgt tgtgtcctct gccgacgtgg ccaaagagat gttcctcaag aaggatcacc
     2461 cgctctccaa ccgcacgatc ccgaatagtg ttacagccgg cgaccaccac aagctcacca
     2521 tgtcttggct cccggtgtct ccgaagtggc gcaacttccg caagattacc gccgtgcatc
     2581 tgctctcccc acagagactc gatgcctgcc agacattcag gcacgccaag gtgcagcagc
     2641 tctacgagta cgttcaagag tgcgcccaga aaggccaggc cgtggatatt ggcaaggccg
     2701 cctttacgac cagcctcaac ctcctcagca agctgttctt cagcgtcgag ctggcgcacc
     2761 acaagtccca taccagccaa gagttcaaag agctgatctg gaacatcatg gaagatatag
     2821 gcaagccgaa ctacgccgac tacttcccga ttctcggctg cgttgaccca tctggcatta
     2881 gaagaaggct cgcctgctcc ttcgacaagc tgatcgccgt gttccagggc atcatctgcg
     2941 agagactcgc cccagattcc tccaccacaa ctaccaccac caccgacgac gtgctcgatg
     3001 tgctcctcca gctgttcaag cagaacgagc tgacgatggg cgagatcaac cacctcctcg
     3061 tggacatctt cgacgccggc accgatacca catcctccac attcgagtgg gtgatgaccg
     3121 agctgatccg caatccagag atgatggaaa aggcccaaga ggaaatcaag caggtcctcg
     3181 gcaaggacaa gcagatccaa gagtccgaca tcatcaacct gccgtacctc caggcgatca
     3241 tcaaagagac actccgcctc catccgccga ccgtgttctt gctcccaaga aaggccgaca
     3301 ccgatgtcga gctgtacggc tacatcgtgc cgaaggatgc ccagatcctc gtgaacctct
     3361 gggccattgg cagggaccca aacgcctggc agaacgccga tattttcagc ccagagcgct
     3421 tcatcggctg cgagatcgat gttaagggcc gcgatttcgg cctccttcca tttggcgctg
     3481 gccgcagaat ttgcccaggc atgaatctcg ccatcaggat gctcaccctc atgctcgcca
     3541 cactcctcca gttcttcaac tggaagctcg aaggcgacat ctccccgaag gacctcgaca
     3601 tggacgagaa gttcggcatt gcgctccaaa agaccaagcc gctcaagctc atcccgattc
     3661 cgcgctacgg tagcggagct accaatttta gcctccttaa gcaggcaggt gatgtagaag
     3721 agaaccccgg gcctatgaag atgatgaacg gcgaggacgc caacgaccag atgatcaaag
     3781 agtccttctt catcacccac ggcaacccga tcctcaccgt cgaggataca catccgctca
     3841 ggccgttctt cgagacatgg cgcgagaaga ttttctccaa gaagccgaag gccatcctca
     3901 tcatctccgg ccactgggag acagtgaagc caaccgtgaa cgccgtgcac atcaacgaca
     3961 ccatccacga cttcgacgac tacccagccg ccatgtacca gttcaagtac ccagctccag
     4021 gcgagccaga gcttgcgaga aaggtggaag agatcctcaa gaagtccggg ttcgagacag
     4081 ccgagacaga ccaaaagagg ggccttgatc acggcgcctg ggttccactc atgctcatgt
     4141 atccagaggc ggacatcccg gtgtgccagc tctcagttca gccacatctc gacggcacct
     4201 accactacaa tctcggcaga gccctcgcgc cgctcaagaa tgatggcgtg ctcattattg
     4261 gctccggcag cgccacacat ccactcgatg agacaccgca ctacttcgat ggtgttgccc
     4321 cttgggccgc tgccttcgat tcttggctta ggaaggccct catcaacggc cgcttcgagg
     4381 aagtgaacat ctacgagagc aaggccccga actggaagct cgcccatcca tttccagagc
     4441 acttctaccc gctccacgtt gtgctcggcg ctgctggtga aaagtggaag gccgagctga
     4501 tccactcctc ctgggatcat ggcacacttt gccacggctc ctacaagttc acctccgccg
     4561 gatccggagc aaccaacttt agcctgctca agcaagcagg agatgttgag gaaaatcctg
     4621 gccccatgac cgccatcaag atgaacacca acggcgaggg cgagacacag cacatcctca
     4681 tgatcccgtt catggcgcag ggccacctca ggccatttct cgaactcgcc atgttcctct
     4741 acaagcgctc ccacgtgatc atcaccctgc tcacaactcc gctcaatgcc ggcttcctca
     4801 ggcacctcct tcaccaccat tcctactcct ccagcggcat caggatcgtc gagctgccat
     4861 tcaactccac caaccacgga ctcccaccgg gcatcgagaa caccgataag ctcacactcc
     4921 cgctcgtggt gtccctcttc cattccacca tcagcctcga tccgcacctc cgcgattaca
     4981 tctccaggca tttcagccca gccaggccac cactctgcgt gatccatgat gtgttcctcg
     5041 gctgggttga ccaggtggcc aaggatgtgg gctctacagg cgtggtgttc acaacaggcg
     5101 gcgcttatgg cacatccgcc tacgtgtcca tctggaacga tctcccgcac cagaactact
     5161 ccgacgacca agagttcccg ctgccaggct tcccagagaa ccataagttc cgcaggtccc
     5221 agctccatcg gttcctcaga tatgccgacg gctccgacga ttggtccaag tatttccagc
     5281 cgcagctccg ccagtccatg aagtcttttg gctggctctg caactccgtg gaagagatcg
     5341 agacactcgg cttctccatc ctccgcaact acaccaagct gccgatctgg ggcatcggcc
     5401 cacttattgc ttccccagtg cagcactcct cctccgacaa caattcaaca ggcgccgagt
     5461 tcgtgcagtg gctcagcctc aaagagccgg actccgtcct ctacatctcc ttcggctccc
     5521 agaacacgat cagcccgacg cagatgatgg aactcgctgc tggccttgag tcctccgaga
     5581 agccattcct ctgggtgatc agagccccgt tcggcttcga catcaacgaa gagatgcgcc
     5641 cagagtggct gccagagggc tttgaggaac gcatgaaggt gaagaaacag ggcaagctcg
     5701 tgtacaagct cggcccgcag cttgagatcc tcaaccatga atccatcggc ggctttctca
     5761 cccactgcgg atggaacagc atccttgagt ctcttcgcga gggcgttccg atgcttggat
     5821 ggccacttgc tgccgagcag gcctacaacc tcaagtacct cgaagatgag atgggcgtcg
     5881 cggttgagct tgctagaggc ctcgaaggcg agatctccaa agagaaggtc aagcgcatcg
     5941 tcgagatgat ccttgagcgc aacgagggct ccaaaggctg ggagatgaag aatcgcgccg
     6001 tggaaatggg caaaaagctc aaggacgccg tgaacgagga aaaagagctg aagggctcct
     6061 ccgtgaaggc gatcgacgat ttcctcgacg ccgtcatgca ggccaaactt gagccaagcc
     6121 tccagtgata gtgaatatga agatgaagat gaaatatttg gtgtgtcaaa taaaaaggtt
     6181 gtgtgcttaa gtttgtgttt ttttcttggc ttgttgtgtt atgaatttgt ggctttttct
     6241 aatattaaat gaatgtaaca tctcattata atgaataaac aaatgtttct ataatccatt
     6301 gtgaatgttt tgttggatct cttctccagc atataactac tgtatgtgct atggtatgga
     6361 ctatggaata tgattaaaga taagacctaa ctgagtaagc tagcttgttc gagtattatg
     6421 gcattgggaa aactgttttt cttgtaccat ttgttgtgct tgtaatttac tgtgtttttt
     6481 attcggtttt cgctatcgaa ctgtgaaatg gaaatggatg gagaagagtt aatgaatgat
     6541 atggtccttt tgttcattct caaattaata ttatttgttt tttctcttat ttgttgtgtg
     6601 ttgaatttga aattataaga gatatgcaaa cattttgttt tgagtaaaaa tgtgtcaaat
     6661 cgtggcctct aatgaccgaa gttaatatga ggagtaaaac actagatccc caaacaagct
     6721 tggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     6781 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     6841 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ctagagcagc ttgccaacat
     6901 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
     6961 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
     7021 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
     7081 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
     7141 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
     7201 aaagcaagtg gattgatgtg ataacatggt ggagcacgac actctcgtct actccaagaa
     7261 tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac aaagggtaat
     7321 atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca aaaggacagt
     7381 agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg ctatcgttca
     7441 agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga
     7501 aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata tctccactga
     7561 cgtaagggat gacgcacaat cccactatcc ttcgcaagac ccttcctcta tataaggaag
     7621 ttcatttcat ttggagagga cacgctgaaa tcaccagtct ctctctacaa atctatctct
     7681 ctcgagcttt tcgcagatct gtcgatcgac catggtacgc agcagcaacg atgttacgca
     7741 gcagggcagt cgccctaaaa caaagttagg tggctcaagt atgggcatca ttcgcacatg
     7801 taggctcggc cctgaccaag tcaaatccat gcgggctgct cttgatcttt tcggtcgtga
     7861 gttcggagac gtagccacct actcccaaca tcagccggac tccgattacc tcgggaactt
     7921 gctccgtagt aagacattca tcgcgcttgc tgccttcgac caagaagcgg ttgttggcgc
     7981 tctcgcggct tacgttctgc ccaagtttga gcagccgcgt agtgagatct atatctatga
     8041 tctcgcagtc tccggcgagc accggaggca gggcattgcc accgcgctca tcaatctcct
     8101 caagcatgag gccaacgcgc ttggtgctta tgtgatctac gtgcaagcag attacggtga
     8161 cgatcccgca gtggctctct atacaaagtt gggcatacgg gaagaagtga tgcactttga
     8221 tatcgaccca agtaccgcca cctaacaatt cgttcaagcc gagatcggct tcccggccta
     8281 gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc cagataaggg
     8341 aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc cttagtatgt
     8401 atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac caaaatccag
     8461 tactaaaatc cagatccccc gaattaattc ggcgttaatt cagtacatta aaaacgtccg
     8521 caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc
     8581 agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag
     8641 cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat
     8701 gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc
     8761 tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc
     8821 gggcacgaac ccagtggaca taagcctcgt tcggttcgta agctgtaatg caagtagcgt
     8881 aactgccgtc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag
     8941 tggcggtttt catggcttct tgttatgaca tgtttttttg gggtacagtc tatgcctcgg
     9001 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac
     9061 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg
     9121 atcgccgaag tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa
     9181 ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac
     9241 agtgatattg atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct
     9301 ttgatcaacg accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct
     9361 gtagaagtca ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc
     9421 gaactgcaat ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc
     9481 acgatcgaca ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg
     9541 gtaggtccag cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg
     9601 ctaaatgaaa ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat
     9661 gtagtgctta cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag
     9721 gatgtcgctg ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt
     9781 gaagctagac aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag
     9841 ttggaagaat ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt
     9901 ctagctagaa attcgttcaa gccgacgccg cttcgccggc gttaactcaa gcgattagat
     9961 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt
    10021 taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc
    10081 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    10141 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    10201 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    10261 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
    10321 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    10381 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
    10441 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
    10501 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
    10561 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
    10621 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
    10681 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
    10741 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
    10801 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
    10861 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
    10921 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag
    10981 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac
    11041 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
    11101 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
    11161 aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg
    11221 gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc
    11281 catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg
    11341 cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta
    11401 tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa
    11461 atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg
    11521 ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta
    11581 cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc
    11641 tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta
    11701 gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag
    11761 gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct
    11821 gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg
    11881 gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg
    11941 aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag
    12001 ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat
    12061 caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg
    12121 catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt
    12181 tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg
    12241 gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac
    12301 cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac
    12361 ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt
    12421 cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata
    12481 gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg
    12541 cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca
    12601 cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt
    12661 caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt
    12721 gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag
    12781 acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg
    12841 gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat
    12901 ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag
    12961 ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc
    13021 cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc
    13081 agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat
    13141 tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg
    13201 acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca
    13261 gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg
    13321 gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca
    13381 aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg
    13441 tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct
    13501 gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag
    13561 tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg
    13621 gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa
    13681 tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg
    13741 gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct
    13801 tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt
    13861 tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc
    13921 tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca
    13981 cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga
    14041 tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc
    14101 ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg
    14161 gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg
    14221 gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc
    14281 ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc
    14341 aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag
    14401 ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt
    14461 ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg
    14521 ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt
    14581 cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct
    14641 ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg
    14701 ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc
    14761 ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg
    14821 actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac
    14881 ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac
    14941 tgcactatag cagcggaggg gttggatcaa agtact
//
