LOCUS       pT2_hUbC-dCas9-KRAB-T2 10334 bp ds-DNA     circular     19-AUG-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    36..1247
                     /label="Ubc Promoter"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     1084..1103
                     /label="hUBCpro-F"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1119..1137
                     /label="CRISPRi-seq-F2"
                     /note="sequence: gcgagtgtgttttgtgaag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(1258..1292)
                     /label="UbcP-XbaI-R1"
                     /note="sequence: ttgtagtccatggtggctctagagtcgacctgcag"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     regulatory      1276..1285
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1285..1350
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             1357..1377
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             1387..5490
                     /label="dCas9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer          complement(1626..1646)
                     /label="CRISPRi-seq-R2"
                     /note="sequence: ctaaagatctcctgcaggtag"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          5397..5415
                     /label="CRISPRi-seq-F1"
                     /note="sequence: ctctacaaaggaggtcctg"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             5503..5523
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             5557..5751
                     /label="KRAB"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(5699..5718)
                     /label="CRISPRi-seq-R1"
                     /note="sequence: gaggatcacatctggcttag"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             5818..5838
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             5845..5898
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     complement(5899..5918)
                     /label="Puro-R"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             5899..6498
                     /label="PuroR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer_bind     6395..6415
                     /label="Puro-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer          6538..6561
                     /label="AAXBB-EZ-F1"
                     /note="sequence: ccggtGGCGCGCCCGGGTGATCAa"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          complement(6542..6565)
                     /label="AAXBB-EZ-R1"
                     /note="sequence: gatctTGATCACCCGGGCGCGCCa"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             6561..6578
                     /label="GFP CDS"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          complement(6654..6672)
                     /label="IR-DR(R)-seq-R1"
                     /note="sequence: gcagcttataatggttaca"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     polyA_signal    6673..6678
                     /label="polyA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     PCR_primer      complement(6709..6727)
                     /label="GCTCTAGAGC CCCCCTGAACCTGAAACAT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     transposon      complement(6871..7098)
                     /label="IR-DR(R)"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        complement(7158..7177)
                     /label="T3"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     rep_origin      7418..8298
                     /label="ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             complement(8362..9219)
                     /label="Amp"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     rep_origin      9350..9806
                     /label="F1 ori"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     promoter        9972..9992
                     /label="T7"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     transposon      10017..10243
                     /label="IR-DR(L)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          10224..10244
                     /label="IR-DR(L)-seqF"
                     /note="sequence: cagaagtttacatacactaag"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
ORIGIN
        1 ggccgcatta attaacccgt gtcggctcca gatctggcct ccgcgccggg ttttggcgcc
       61 tcccgcgggc gcccccctcc tcacggcgag cgctgccacg tcagacgaag ggcgcagcga
      121 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc
      181 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc
      241 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc
      301 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt
      361 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg
      421 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg
      481 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga
      541 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag
      601 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca
      661 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc
      721 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga
      781 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg cggtgccgtt gggcagtgca
      841 cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt
      901 ataatgcagg gtggggccac ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga
      961 cgcagggttc gggcctaggg taggctctcc tgaatcgaca ggcgccggac ctctggtgag
     1021 gggagggata agtgaggcgt cagtttcttt ggtcggtttt atgtacctat cttcttaagt
     1081 agctgaagct ccggttttga actatgcgct cggggttggc gagtgtgttt tgtgaagttt
     1141 tttaggcacc ttttgaaatg taatcatttg ggtcaatatg taattttcag tgttagacta
     1201 gtaaattgtc cgctaaattc tggccgtttt tggctttttt gttagacgaa gcttgggctg
     1261 caggtcgact ctagagccac catggactac aaagaccatg acggtgatta taaagatcat
     1321 gacatcgatt acaaggatga cgatgacaag atggccccca agaagaagag gaaggtgggc
     1381 cgcggaatgg acaagaagta ctccattggg ctcgccatcg gcacaaacag cgtcggctgg
     1441 gccgtcatta cggacgagta caaggtgccg agcaaaaaat tcaaagttct gggcaatacc
     1501 gatcgccaca gcataaagaa gaacctcatt ggcgccctcc tgttcgactc cggggaaacc
     1561 gccgaagcca cgcggctcaa aagaacagca cggcgcagat atacccgcag aaagaatcgg
     1621 atctgctacc tgcaggagat ctttagtaat gagatggcta aggtggatga ctctttcttc
     1681 cataggctgg aggagtcctt tttggtggag gaggataaaa agcacgagcg ccacccaatc
     1741 tttggcaata tcgtggacga ggtggcgtac catgaaaagt acccaaccat atatcatctg
     1801 aggaagaagc ttgtagacag tactgataag gctgacttgc ggttgatcta tctcgcgctg
     1861 gcgcatatga tcaaatttcg gggacacttc ctcatcgagg gggacctgaa cccagacaac
     1921 agcgatgtcg acaaactctt tatccaactg gttcagactt acaatcagct tttcgaagag
     1981 aacccgatca acgcatccgg agttgacgcc aaagcaatcc tgagcgctag gctgtccaaa
     2041 tcccggcggc tcgaaaacct catcgcacag ctccctgggg agaagaagaa cggcctgttt
     2101 ggtaatctta tcgccctgtc actcgggctg acccccaact ttaaatctaa cttcgacctg
     2161 gccgaagatg ccaagcttca actgagcaaa gacacctacg atgatgatct cgacaatctg
     2221 ctggcccaga tcggcgacca gtacgcagac ctttttttgg cggcaaagaa cctgtcagac
     2281 gccattctgc tgagtgatat tctgcgagtg aacacggaga tcaccaaagc tccgctgagc
     2341 gctagtatga tcaagcgcta tgatgagcac caccaagact tgactttgct gaaggccctt
     2401 gtcagacagc aactgcctga gaagtacaag gaaattttct tcgatcagtc taaaaatggc
     2461 tacgccggat acattgacgg cggagcaagc caggaggaat tttacaaatt tattaagccc
     2521 atcttggaaa aaatggacgg caccgaggag ctgctggtaa agcttaacag agaagatctg
     2581 ttgcgcaaac agcgcacttt cgacaatgga agcatccccc accagattca cctgggcgaa
     2641 ctgcacgcta tcctcaggcg gcaagaggat ttctacccct ttttgaaaga taacagggaa
     2701 aagattgaga aaatcctcac atttcggata ccctactatg taggccccct cgcccgggga
     2761 aattccagat tcgcgtggat gactcgcaaa tcagaagaga ccatcactcc ctggaacttc
     2821 gaggaagtcg tggataaggg ggcctctgcc cagtccttca tcgaaaggat gactaacttt
     2881 gataaaaatc tgcctaacga aaaggtgctt cctaaacact ctctgctgta cgagtacttc
     2941 acagtttata acgagctcac caaggtcaaa tacgtcacag aagggatgag aaagccagca
     3001 ttcctgtctg gagagcagaa gaaagctatc gtggacctcc tcttcaagac gaaccggaaa
     3061 gttaccgtga aacagctcaa agaagactat ttcaaaaaga ttgaatgttt cgactctgtt
     3121 gaaatcagcg gagtggagga tcgcttcaac gcatccctgg gaacgtatca cgatctcctg
     3181 aaaatcatta aagacaagga cttcctggac aatgaggaga acgaggacat tcttgaggac
     3241 attgtcctca cccttacgtt gtttgaagat agggagatga ttgaagaacg cttgaaaact
     3301 tacgctcatc tcttcgacga caaagtcatg aaacagctca agaggcgccg atatacagga
     3361 tgggggcggc tgtcaagaaa actgatcaat gggatccgag acaagcagag tggaaagaca
     3421 atcctggatt ttcttaagtc cgatggattt gccaaccgga acttcatgca gttgatccat
     3481 gatgactctc tcacctttaa ggaggacatc cagaaagcac aagtttctgg ccagggggac
     3541 agtcttcacg agcacatcgc taatcttgca ggtagcccag ctatcaaaaa gggaatactg
     3601 cagaccgtta aggtcgtgga tgaactcgtc aaagtaatgg gaaggcataa gcccgagaat
     3661 atcgttatcg agatggcccg agagaaccaa actacccaga agggacagaa gaacagtagg
     3721 gaaaggatga agaggattga agagggtata aaagaactgg ggtcccaaat ccttaaggaa
     3781 cacccagttg aaaacaccca gcttcagaat gagaagctct acctgtacta cctgcagaac
     3841 ggcagggaca tgtacgtgga tcaggaactg gacatcaatc ggctctccga ctacgacgtg
     3901 gatgccatcg tgccccagtc ttttctcaaa gatgattcta ttgataataa agtgttgaca
     3961 agatccgata aaaatagagg gaagagtgat aacgtcccct cagaagaagt tgtcaagaaa
     4021 atgaaaaatt attggcggca gctgctgaac gccaaactga tcacacaacg gaagttcgat
     4081 aatctgacta aggctgaacg aggtggcctg tctgagttgg ataaagccgg cttcatcaaa
     4141 aggcagcttg ttgagacacg ccagatcacc aagcacgtgg cccaaattct cgattcacgc
     4201 atgaacacca agtacgatga aaatgacaaa ctgattcgag aggtgaaagt tattactctg
     4261 aagtctaagc tggtctcaga tttcagaaag gactttcagt tttataaggt gagagagatc
     4321 aacaattacc accatgcgca tgatgcctac ctgaatgcag tggtaggcac tgcacttatc
     4381 aaaaaatatc ccaagcttga atctgaattt gtttacggag actataaagt gtacgatgtt
     4441 aggaaaatga tcgcaaagtc tgagcaggaa ataggcaagg ccaccgctaa gtacttcttt
     4501 tacagcaata ttatgaattt tttcaagacc gagattacac tggccaatgg agagattcgg
     4561 aagcgaccac ttatcgaaac aaacggagaa acaggagaaa tcgtgtggga caagggtagg
     4621 gatttcgcga cagtccggaa ggtcctgtcc atgccgcagg tgaacatcgt taaaaagacc
     4681 gaagtacaga ccggaggctt ctccaaggaa agtatcctcc cgaaaaggaa cagcgacaag
     4741 ctgatcgcac gcaaaaaaga ttgggacccc aagaaatacg gcggattcga ttctcctaca
     4801 gtcgcttaca gtgtactggt tgtggccaaa gtggagaaag ggaagtctaa aaaactcaaa
     4861 agcgtcaagg aactgctggg catcacaatc atggagcgat caagcttcga aaaaaacccc
     4921 atcgactttc tcgaggcgaa aggatataaa gaggtcaaaa aagacctcat cattaagctt
     4981 cccaagtact ctctctttga gcttgaaaac ggccggaaac gaatgctcgc tagtgcgggc
     5041 gagctgcaga aaggtaacga gctggcactg ccctctaaat acgttaattt cttgtatctg
     5101 gccagccact atgaaaagct caaagggtct cccgaagata atgagcagaa gcagctgttc
     5161 gtggaacaac acaaacacta ccttgatgag atcatcgagc aaataagcga attctccaaa
     5221 agagtgatcc tcgccgacgc taacctcgat aaggtgcttt ctgcttacaa taagcacagg
     5281 gataagccca tcagggagca ggcagaaaac attatccact tgtttactct gaccaacttg
     5341 ggcgcgcctg cagccttcaa gtacttcgac accaccatag acagaaagcg gtacacctct
     5401 acaaaggagg tcctggacgc cacactgatt catcagtcaa ttacggggct ctatgaaaca
     5461 agaatcgacc tctctcagct cggtggagac agcagggctg accccaagaa gaagaggaag
     5521 gtggctagcg atgctaagtc actgactgcc tggtcccgga cactggtgac cttcaaggat
     5581 gtgtttgtgg acttcaccag ggaggagtgg aagctgctgg acactgctca gcagatcctg
     5641 tacagaaatg tgatgctgga gaactataag aacctggttt ccttgggtta tcagcttact
     5701 aagccagatg tgatcctccg gttggagaag ggagaagagc cctggctggt ggagagagaa
     5761 attcaccaag agacccatcc tgattcagag actgcatttg aaatcaaatc atcagttccg
     5821 aaaaagaaac gcaaagttgc tagcgagggc agaggaagtc ttctaacatg cggtgacgtg
     5881 gaggagaatc ccggccctat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5941 gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     6001 cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     6061 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     6121 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     6181 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     6241 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtgtc gcccgaccac
     6301 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6361 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6421 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6481 cgcaagcccg gtgcctgacc agcacactgg cggccgttac tagcttctgc agcacgaccg
     6541 gtGGCGCGCC CGGGTGATCA agatctcgag ctcgatgagt ttggacaaac cacaactaga
     6601 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     6661 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     6721 cagggggagg tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtacttaa
     6781 gagggatcga taaggatcta gcttgtggaa ggctactcga aatgtttgac ccaagttaaa
     6841 caatttaaag gcaatgctac caaatactaa ttgagtgtat gtaaacttct gacccactgg
     6901 gaatgtgatg aaagaaataa aagctgaaat gaatcattct ctctactatt attctgatat
     6961 ttcacattct taaaataaag tggtgatcct aactgaccta agacagggaa tttttactag
     7021 gattaaatgt caggaattgt gaaaaagtga gtttaaatgt atttggctaa ggtgtatgta
     7081 aacttccgac ttcaactgta tagggatcct ctagctagag tcgacctcga gggggggccc
     7141 ggtacccagc ttttgttccc tttagtgagg gttaatttcg agcttggcgt aatcatggtc
     7201 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7261 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7321 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7381 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7441 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7501 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7561 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7621 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7681 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7741 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7801 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7861 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7921 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7981 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     8041 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8101 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8161 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8221 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8281 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8341 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8401 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8461 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8521 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8581 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8641 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8701 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8761 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8821 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8881 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8941 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     9001 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9061 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9121 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9181 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9241 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9301 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgcgccctg
     9361 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     9421 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     9481 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     9541 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     9601 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     9661 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     9721 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     9781 taacaaaata ttaacgctta caatttccat tcgccattca ggctgcgcaa ctgttgggaa
     9841 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
     9901 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
     9961 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg agctcggatc cctatacagt
    10021 tgaagtcgga agtttacata cacttaagtt ggagtcatta aaactcgttt ttcaactact
    10081 ccacaaattt cttgttaaca aacaatagtt ttggcaagtc agttaggaca tctactttgt
    10141 gcatgacaca agtcattttt ccaacaattg tttacagaca gattatttca cttataattc
    10201 actgtatcac aattccagtg ggtcagaagt ttacatacac taagttgact gtgcctttaa
    10261 acagcttgga aaattccaga aaatgatgtc atggctttag aagcttgata tccatggaat
    10321 tcactagtgc gcgc
//