LOCUS       pU6-hL1-5’_3.3kb        6314 bp ds-DNA     circular     19-AUG-2024
DEFINITION  .
COMMENT     use XbaI and NotI, EZ cut  insertion:  cut 3.3kb 5' site of hLINE1
            from EF06R plsmid backbone: cut 3.0kb backbone from Addgene 64216
            pU6-sgRosa26-1_CBh-Cas9-T2A-BFP plasmid --> T4 ligation to do
            subclone Plasmid used for LINE1 DNA-FISH probe generation.
FEATURES             Location/Qualifiers
     Promoter        1253..1501
                     /label="hU6"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer          1441..1459
                     /label="Addgn6424-seq-F2"
                     /note="sequence: GCTTACCGTAACTTGAAAG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     Misc._feature   1508..1519
                     /label="sgRosa26-1"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1520..2799)
                     /label="hL1-ORF2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2863..3898)
                     /label="hL1-ORF1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(3899..4785)
                     /label="hL1-5UTR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(4836..4852)
                     /label="Addgn6424-seq-R1"
                     /note="sequence: CCAACTCCATCACTAGG"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             5886..432
                     /label="ampR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
ORIGIN
        1 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG
       61 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA
      121 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA
      181 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT
      241 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGAAG CCGCGGTATC
      301 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG
      361 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT
      421 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT
      481 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC
      541 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
      601 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
      661 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
      721 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
      781 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
      841 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
      901 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
      961 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     1021 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     1081 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     1141 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     1201 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTCCCCACGA
     1261 GGGCCTATTT CCCATGATTC CTTCATATTT GCATATACGA TACAAGGCTG TTAGAGAGAT
     1321 AATTGGAATT AATTTGACTG TAAACACAAA GATATTAGTA CAAAATACGT GACGTAGAAA
     1381 GTAATAATTT CTTGGGTAGT TTGCAGTTTT AAAATTATGT TTTAAAATGG ACTATCATAT
     1441 GCTTACCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
     1501 AAACACCGAC TCCAGTCTTt ctagattttc tagtttattt gcgtagaggt gtttgtagta
     1561 ttctctgatg gtagtttgta tttctgtggg atcggtggtg atatcccctt tatcattttt
     1621 tattgtgtct atttgattct tctctctttt tttctttatt agtcttgcta gcggtctatc
     1681 aattttgttg atcctttcaa aaaaccagct cctggattca ttgatttttt gaagggtttt
     1741 ttgtgtctct atttccttca gttctgctct gattttagtt atttcttgcc ttctgctagc
     1801 ttttgaatgt gtttgctctt gcttttctag ttcttttaat tgtgatgtta gggtgtcaat
     1861 tttggatctt tcctgctttc tcttgtaggc atttagtgct ataaatttcc ctctacatac
     1921 tgctttgaat gcgtcccaga gattctggta tgtggtgtct ttgttctcgt tggtttcaaa
     1981 gaacatcttt atttctgcct tcatttcgtt atgtacccag tagtcattca ggagcaggtt
     2041 gttcagtttc catgtagttg agcggctttg agtgagattc ttaatcctga gctctagttt
     2101 gattgcactg tggtctgaga gatagtttgt tataatttct gttcttttac atttgctgag
     2161 gagagctttg cttccaacta tgtggtcaat tttggaatag gtgtggtgtg gtgctgaaaa
     2221 aaacgtatat tctgttgatt tggggtggag agttctgtag atgtctatta ggtctgcttg
     2281 gtgcagagct gagttcaatt cctgggtatc cttgttgact ttctgtctcg ttgatctgtc
     2341 taatgttgac agtggggtgt taaagtctcc cattattaat gtgtgggagt ctaagtctct
     2401 ttgtaggtca ctgaggactt gctttatgaa tctgggtgct cctgtattgg gtgcataaat
     2461 atttaggata gttagctcct cttgttgaat tgatcccttt accattatgt aatggccttc
     2521 tttgtctctt ttgatctttg ttggtttaaa gtctgtttta tcagagacta ggattgcaac
     2581 ccctgccttt ttttgttttc cattggcttg gtagatcttc ctccatcctt ttattttgag
     2641 cctatgtgtg tctctgcacg tgagatgggt ttcctgaata cagcacactg atgggtcttg
     2701 actctttatc caacttgcca gtctgtgtct tttaattgca gaatttagtc catttatatt
     2761 taaagttaat attgttatgt gtgaagttga tcctgtcatt atgatgttag ctggtgattt
     2821 tgctcattag ttggtgcagt ttcttcctag tcttgatggt ctttacattt tggcatgatt
     2881 ttgcagcggc tggtaccggt tgttcctttc catgtttagc gcttccttca ggagctcttt
     2941 tagggcaggc ctggtggtga caaaatctct caacatttgc ttgtctataa agtattttat
     3001 ttctccttca cttatgaagc ttagtttggc tggatatgaa attctgggtt gaaaattctt
     3061 ttctttaaga atgttgaata ttggccccca ctctcttctg gcttgtaggg tttctgccga
     3121 gagatccgct gttagtctga tgggctttcc tttgagggta acccgacctt tctctctggc
     3181 tgcccttaac attttttcct tcatttcaac tttggtgaat ctgacaatta tgtgtcttgg
     3241 agttgctctt ctcgaggagt atctttgtgg cgttctctgt atttcctgaa tctgaacgtt
     3301 ggcctgcctt gctagattgg ggaagttctc ctggataata tcctgcagag tgttttccaa
     3361 cttggttcca ttctccacat cactttcagg tacaccaatc agacgtagat ttggtctttt
     3421 cacatagtcc catatttctt ggaggctttg ctcatttctt tttattcttt tttctctaaa
     3481 cttcccttct cgcttcattt cattcatttc atcttccatt gctgataccc tttcttccag
     3541 ttgatcgcat cggctcctga ggcttctgca ttcttcacgt agttctcgag ccttggtttt
     3601 cagctccatc agctccttta agcacttctc tgtattggtt attctagtta tacattcttc
     3661 taaatttttt tcaaagtttt caacttcttt gcctttggtt tgaatgtcct cccgtagctc
     3721 agagtaattt gatcgtctga agccttcttc tctcagctca tcaaaatcat tctccatcca
     3781 gctttgttct gttgctggtg aggaactgcg ttcctttgga ggaggagagg cgctctgcgt
     3841 tttagagttt ccagtttttc tgttctgttt tttccccatc tttgtggttt tatctacttt
     3901 tggtctttga tgatggtgat gtacagatgg gttttcggtg tagatgtcct ttctggttgt
     3961 tagttttcct tctaacagac aggaccctca gctgcaggtc tgttggaata ccctgccgtg
     4021 tgaggtgtca gtgtgcccct gctggggggt gcctcccagt taggctgctc gggggtcagg
     4081 ggtcagggac ccacttgagg aggcagtctg cccgttctca gatctccagc tgcgtgctgg
     4141 gagaaccact gctctcttca aagctgtcag acagggacac ttaagtctgc agaggttact
     4201 gctgtctttt tgtttgtctg tgccctgccc ccagaggtgg agcctacaga ggcaggcagg
     4261 cctccttgag ctgtggtggg ctccacccag ttcgagcttc cctgctgctt tgtttaccta
     4321 agcaagcctg ggcaatggcg ggcgcccctc ccccagcctc gttgccgcct tgcagtttga
     4381 tctcagactg ctgtgctagc aatcagcgag attccgtggg cgtaggaccc tccgagccag
     4441 gtgcgggata tagtctcgtg gtgcgccgtt tcttaagccg gtctgaaaag cgcaatattc
     4501 gggtgggagt gacccgattt tccaggtgcg tccgtcaccc ctttctttga ctcggaaagg
     4561 gaactccctg accccttgcg cttcccaggt gaggcaatgc ctcgccctgc ttcggctcgc
     4621 gcacggtgcg cacacacact ggcctgcgcc cactgtctgg cactccctag tgagatgaac
     4681 ccggtacctc agatggaaat gcagaaatca ccgtcttctg cgtcgctcac gctgggagct
     4741 gtagaccgga gctgttccta ttcggccatc ttggctcctc ccccccacct tacagtctta
     4801 atacacattc cagggctaga gcggccgcAG GAACCCCTAG TGATGGAGTT GGCCACTCCC
     4861 TCTCTGCGCG CTCGCTCGCT CACTGAGGCC GGGCGACCAA AGGTCGCCCG ACGCCCGGGC
     4921 TTTGCCCGGG CGGCCTCAGT GAGCGAGCGA GCGCGCAGCT GCCTGCAGGG GCGCCTGATG
     4981 CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATACGTCA AAGCAACCAT
     5041 AGTACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA
     5101 CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG
     5161 CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT
     5221 TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTT GGGTGATGGT TCACGTAGTG
     5281 GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA
     5341 GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGGCTAT TCTTTTGATT
     5401 TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT
     5461 TTAACGCGAA TTTTAACAAA ATATTAACGT TTACAATTTT ATGGTGCACT CTCAGTACAA
     5521 TCTGCTCTGA TGCCGCATAG TTAAGCCAGC CCCGACACCC GCCAACACCC GCTGACGCGC
     5581 CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA
     5641 GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGACGA AAGGGCCTCG
     5701 TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG
     5761 GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA
     5821 ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA
     5881 AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC
     5941 TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG
     6001 GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC
     6061 GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT
     6121 TATCCCGTAT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG
     6181 ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG
     6241 AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA
     6301 CGATCGGAGG ACCG
//