LOCUS       pKN393                  7408 bp ds-DNA     circular     10-APR-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     primer          336..360
                     /label="KN_124"
                     /note="sequence: CGTTACATAACTTACGGTAAATGGC"
     misc_feature    336..639
                     /label="CMV Enhancer"
     misc_feature    640..843
                     /label="CMV Promoter"
     misc_feature    888..907
                     /label="T7"
     primer          888..911
                     /label="KN_228"
                     /note="sequence: TAATACGACTCACTATAGGGAGAC"
     primer          888..946
                     /label="KN_653"
                     /note="sequence: TAATACGACTCACTATAGGGAGACCCAAGCTTatgggcccaaaaaagaaaagaaaagtt"
     primer          908..949
                     /label="LH106"
                     /note="sequence: AGACCCAAGCTTatgggcccaaaaaagaaaagaaaagttggc"
     CDS             920..1420
                     /label="Translation 920-1420"
                     /translation="MGPKKKRKVGYPYDVPDYAIEGRHMLAVKMASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKGAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIYADSRRDPATVTGYRLFEEIL*"
     CDS             926..946
                     /label="SV40 NLS"
     CDS             950..976
                     /label="HA"
     CDS             977..988
                     /label="Factor Xa site"
     misc_feature    989..1009
                     /label="Linker"
     misc_feature    1010..1357
                     /label="MS2"
     misc_feature    1358..1384
                     /label="Linker"
     primer          complement(1370..1417)
                     /label="KN_646"
                     /note="sequence: CAGAATCTCCTCAAAcagGCGGTAGCCGGTCACGGTGGCGGGATCCCG"
     primer          complement(1370..1417)
                     /label="KN_646"
                     /note="sequence: CAGAATCTCCTCAAAcagGCGGTAGCCGGTCACGGTGGCGGGATCCCG"
     misc_feature    1385..1417
                     /label="SmBit 114"
     primer          1385..1438
                     /label="KN_647"
                     /note="sequence: GTGACCGGCTACCGCctgTTTGAGGAGATTCTGTAACGTACGACGCGTGGATCC"
     misc_feature    1436..2003
                     /label="IRES"
     primer          1453..1470
                     /label="KN_025"
                     /note="sequence: ACGTTACTGGCCGAAGCC"
     primer          complement(1453..1470)
                     /label="KN_108"
                     /note="sequence: ggcttcggccagtaacgt"
     primer          complement(1968..1992)
                     /label="KN_034"
                     /note="sequence: TTTTTCAAAGGAAAACCACGTCCCC"
     primer          1983..2042
                     /label="KN_656"
                     /note="sequence: CTTTGAAAAACACGATGATAATATGGCCACAGCCTGCAGGatgggctccaaaaccatcgt"
     primer          complement(2004..2063)
                     /label="KN_655"
                     /note="sequence: gtagcctcgccgaccgaaagaacgatggttttggagcccatCCTGCAGGCTGTGGCCATA"
     CDS             2023..2391
                     /label="Translation 2023-2391"
                     /translation="MGSKTIVLSVGEATRTLTEIQSTADRQIFEEKVGPLVGRLRLTASLRQNGAKTAYRVNLKLDQADVVDSGLPKVRYTQVWSHDVTIVANSTEASRKSLYDLTKSLVATSQVEDLVVNLVPLGR"
     CDS             2029..2391
                     /label="PP7"
     CDS             2389..2421
                     /label="Translation 2389-2421"
                     /translation="RADPPVCIATA"
     CDS             2392..2421
                     /label="Linker"
     primer          complement(2392..2421)
                     /label="KN_681"
                     /note="sequence: GGCGGTGGCGATGCATaccggtggatccgc"
     primer          complement(2407..2451)
                     /label="KN_638"
                     /note="sequence: CCAGTCCCCAACGAAATCTTCGAGTGTGAAGGCGGTGGCGATGCA"
     primer          2422..2441
                     /label="KN_573"
                     /note="sequence: TTCACACTCGAAGATTTCGT"
     primer          complement(2422..2451)
                     /label="KN_603"
                     /note="sequence: CCAGTCCCCAACGAAATCTTCGAGTGTGAA"
     misc_feature    2422..2892
                     /label="LgBit NLuc"
     CDS             2422..2892
                     /label="Translation 2422-2892"
                     /translation="FTLEDFVGDWEQTAAYNLDQVLEQGGVSSLLQNLAVSVTPIQRIVRSGENALKIDIHVIIPYEGLSADQMAQIEEVFKVVYPVDDHHFKVILPYGTLVIDGVTPNMLNYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLITPDGSMLFRVTINS"
     primer          complement(2873..2922)
                     /label="KN_639"
                     /note="sequence: CTTGTCATCGTCGTCCTTGTAGTCCTCGAGGCTGTTGATGGTTACTCGGA"
     CDS             2899..2922
                     /label="FLAG"
     CDS             2899..2922
                     /label="Translation 2899-2922"
                     /translation="DYKDDDDK"
     primer          complement(2899..2958)
                     /label="KN_654"
                     /note="sequence: CGGCCGCCAGTGTGATGGATATCTGCAGAATTCTTACTTGTCATCGTCGTCCTTGTAGTC"
     CDS             2923..2925
                     /label="Translation 2923-2925"
                     /translation="*"
     primer          2926..2952
                     /label="AL017_pcDNA_NTR_fwd"
                     /note="sequence: GAATTCTGCAGATATCCATCACACTGG"
     primer          2926..2956
                     /label="KN_126"
                     /note="sequence: GAATTCTGCAGATATCCATCACACTGGCGGC"
     CDS             2926..3009
                     /label="Translation 2926-3009"
                     /translation="EFCRYPSHWRPLEHASRGPYSIVSPKC*"
     primer          complement(3280..3299)
                     /label="KN_103"
                     /note="sequence: GGGGATACCCCCTAGAGCCC"
     misc_feature    3317..3623
                     /label="F1 Ori"
     misc_feature    4141..4932
                     /label="KanR"
     primer          4953..4969
                     /label="KN_509"
                     /note="sequence: ATGACCGACCAAGCGAC"
     primer          5673..5693
                     /label="KN_102"
                     /note="sequence: GCGTTTTTCCATAGGCTCCGC"
     primer          6267..6291
                     /label="KN_315"
                     /note="sequence: GAAGATCCTTTGATCTTTTCTACGG"
     primer          6440..6459
                     /label="KN_302"
                     /note="sequence: CCAATGCTTAATCAGTGAGG"
     misc_feature    complement(6440..7099)
                     /label="AmpR"
     primer          7100..7119
                     /label="KN_240"
                     /note="sequence: CATTGGAAAACGTTCTTCGG"
ORIGIN
        1 TCCCCGAAAA GTGCCACCTG ACGTCGACGG ATCGGGAGAT CTCCCGATCC CCTATGGTGC
       61 ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATCTGCT CCCTGCTTGT
      121 GTGTTGGAGG TCGCTGAGTA GTGCGCGAGC AAAATTTAAG CTACAACAAG GCAAGGCTTG
      181 ACCGACAATT GCATGAAGAA TCTGCTTAGG GTTAGGCGTT TTGCGCTGCT TCGCGATGTA
      241 CGGGCCAGAT ATACGCGTTG ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG
      301 GGGTCATTAG TTCATAGCCC ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC
      361 CCGCCTGGCT GACCGCCCAA CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC
      421 ATAGTAACGC CAATAGGGAC TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT
      481 GCCCACTTGG CAGTACATCA AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT
      541 GACGGTAAAT GGCCCGCCTG GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT
      601 TGGCAGTACA TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC
      661 ATCAATGGGC GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC
      721 GTCAATGGGA GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC
      781 TCCGCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA
      841 GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA TACGACTCAC
      901 TATAGGGAGA CCCAAGCTTa tgggcccaaa aaagaaaaga aaagttggct acccctacga
      961 cgtgcccgac tacgccatcg aaggccgcca tatgctagcc gttaaaatgg cttctaactt
     1021 tactcagttc gttctcgtcg acaatggcgg aactggcgac gtgactgtcg ccccaagcaa
     1081 cttcgctaac gggatcgctg aatggatcag ctctaactcg cgttcacagg cttacaaagt
     1141 aacctgtagc gttcgtcaga gctctgcgca gaatcgcaaa tacaccatca aagtcgaggt
     1201 gcctaaaggc gcctggcgtt cgtacttaaa tatggaacta accattccaa ttttcgccac
     1261 gaattccgac tgcgagctta ttgttaaggc aatgcaaggt ctcctaaaag atggaaaccc
     1321 gattccctca gcaatcgcag caaactccgg catctacgcg gattctagaC GGGATCCCGC
     1381 CACCGTGACC GGCTACCGCc tgTTTGAGGA GATTCTGTAA CGTACGACGC GTGGATCCCT
     1441 CCCCCCCCCC TAACGTTACT GGCCGAAGCC GCTTGGAATA AGGCCGGTGT GCGTTTGTCT
     1501 ATATGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC
     1561 CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC
     1621 TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TTGAAGACAA ACAACGTCTG
     1681 TAGCGACCCT TTGCAGGCAG CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGCCAAA
     1741 AGCCACGTGT ATAAGATACA CCTGCAAAGG CGGCACAACC CCAGTGCCAC GTTGTGAGTT
     1801 GGATAGTTGT GGAAAGAGTC AAATGGCTCT CCTCAAGCGT ATTCAACAAG GGGCTGAAGG
     1861 ATGCCCAGAA GGTACCCCAT TGTATGGGAT CTGATCTGGG GCCTCGGTGC ACATGCTTTA
     1921 CATGTGTTTA GTCGAGGTTA AAAAAACGTC TAGGCCCCCC GAACCACGGG GACGTGGTTT
     1981 TCCTTTGAAA AACACGATGA TAATATGGCC ACAGCCTGCA GGatgggctc caaaaccatc
     2041 gttctttcgg tcggcgaggc tactcgcact ctgactgaga tccagtccac cgcagaccgt
     2101 cagatcttcg aagagaaggt cgggcctctg gtgggtcggc tgcgcctcac ggcttcgctc
     2161 cgtcaaaacg gagccaagac cgcgtatcgc gtcaacctaa aactggatca ggcggacgtc
     2221 gttgattccg gacttccgaa agtgcgctac actcaggtat ggtcgcacga cgtgacaatc
     2281 gttgcgaata gcaccgaggc ctcgcgcaaa tcgttgtacg atttgaccaa gtccctcgtc
     2341 gcgacctcgc aggtcgaaga tcttgtcgtc aaccttgtgc cgctgggccg tgcggatcca
     2401 ccggtATGCA TCGCCACCGC CTTCACACTC GAAGATTTCG TTGGGGACTG GGAACAGACA
     2461 GCCGCCTACA ACCTGGACCA AGTCCTTGAA CAGGGAGGTG TGTCCAGTTT GCTGCAGAAT
     2521 CTCGCCGTGT CCGTAACTCC GATCCAAAGG ATTGTCCGGA GCGGTGAAAA TGCCCTGAAG
     2581 ATCGACATCC ATGTCATCAT CCCGTATGAA GGTCTGAGCG CCGACCAAAT GGCCCAGATC
     2641 GAAGAGGTGT TTAAGGTGGT GTACCCTGTG GATGATCATC ACTTTAAGGT GATCCTGCCC
     2701 TATGGCACAC TGGTAATCGA CGGGGTTACG CCGAACATGC TGAACTATTT CGGACGGCCG
     2761 TATGAAGGCA TCGCCGTGTT CGACGGCAAA AAGATCACTG TAACAGGGAC CCTGTGGAAC
     2821 GGCAACAAAA TTATCGACGA GCGCCTGATC ACCCCCGACG GCTCCATGCT GTTCCGAGTA
     2881 ACCATCAACA GCCTCGAGGA CTACAAGGAC GACGATGACA AGtaaGAATT CTGCAGATAT
     2941 CCATCACACT GGCGGCCGCT CGAGCATGCA TCTAGAGGGC CCTATTCTAT AGTGTCACCT
     3001 AAATGCTAGA GCTCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT
     3061 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA
     3121 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG
     3181 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC
     3241 GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG GGTATCCCCA
     3301 CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC
     3361 TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC
     3421 GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG
     3481 TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC
     3541 ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG
     3601 ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA
     3661 AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA
     3721 CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA
     3781 GCAGGCAGAA GTATGCAAAG CATGCATCTC AATTAGTCAG CAACCAGGTG TGGAAAGTCC
     3841 CCAGGCTCCC CAGCAGGCAG AAGTATGCAA AGCATGCATC TCAATTAGTC AGCAACCATA
     3901 GTCCCGCCCC TAACTCCGCC CATCCCGCCC CTAACTCCGC CCAGTTCCGC CCATTCTCCG
     3961 CCCCATGGCT GACTAATTTT TTTTATTTAT GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG
     4021 CTATTCCAGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG
     4081 GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG
     4141 ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC
     4201 TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG
     4261 CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG
     4321 GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC
     4381 GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT
     4441 CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG
     4501 CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC
     4561 GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG
     4621 CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC
     4681 GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC
     4741 CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA
     4801 GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC
     4861 GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC
     4921 GAGTTCTTCT GAGCGGGACT CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC
     4981 CATCACGAGA TTTCGATTCC ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT
     5041 TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC
     5101 ACCCCAACTT GTTTATTGCA GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT
     5161 TCACAAATAA AGCATTTTTT TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG
     5221 TATCTTATCA TGTCTGTATA CCGTCGACCT CTAGCTAGAG CTTGGCGTAA TCATGGTCAT
     5281 AGCTGTTTCC TGTGTGAAAT TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA
     5341 GCATAAAGTG TAAAGCCTGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC
     5401 GCTCACTGCC CGCTTTCCAG TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC
     5461 AACGCGCGGG GAGAGGCGGT TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT
     5521 CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC
     5581 GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA
     5641 AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG
     5701 ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA
     5761 GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC
     5821 TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC
     5881 GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC
     5941 CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG
     6001 TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT
     6061 ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA
     6121 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT
     6181 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA
     6241 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG
     6301 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT
     6361 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT
     6421 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC
     6481 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG
     6541 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG
     6601 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT
     6661 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG
     6721 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT
     6781 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA
     6841 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG
     6901 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT
     6961 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA
     7021 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA
     7081 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT
     7141 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT
     7201 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA
     7261 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT
     7321 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA
     7381 ATAAACAAAT AGGGGTTCCG CGCACATT
//