LOCUS       plasmid                 7192 bp    DNA     circular SYN 29-AUG-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             624..1484
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             6028..6747
                     /note="pLannotate"
                     /label="EGFP"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             2997..4379
                     /note="pLannotate"
                     /label="F9"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     repeat_region   6759..6899
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="repeat_region"
     polyA_signal    complement(5772..5906)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        519..623
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_RNA        2733..2808
                     /note="pLannotate"
                     /label="gRNA scaffold"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_RNA"
     rep_origin      1655..2244
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     rep_origin      join(6974..7192,1..237)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        2463..2703
                     /note="pLannotate"
                     /label="U6 promoter"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     misc_feature    complement(6904..6955)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="36.9"
                     /fragment="True"
                     /other="misc_feature"
     misc_feature    complement(238..287)
                     /note="pLannotate"
                     /label="bom (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="35.5"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(389..424)
                     /note="pLannotate"
                     /label="rop (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="18.8"
                     /fragment="True"
                     /other="CDS"
     repeat_region   2317..2344
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
     repeat_region   2373..2400
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="19.9"
                     /fragment="True"
                     /other="repeat_region"
ORIGIN
        1 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
       61 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aactctatct
      121 cgggctattc ttttgattta taagggattt tgccgatttc ggtctattgg ttaaaaaatg
      181 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat
      241 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
      301 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
      361 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
      421 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      481 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      601 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      661 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      721 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      781 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
      841 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
      901 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
      961 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     1021 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     1081 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     1141 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     1201 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     1261 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     1321 ctggagccgg tgagcgtgga agccgcggta tcattgcagc actggggcca gatggtaagc
     1381 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     1441 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     1501 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     1561 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     1621 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt tctgcgcgta
     1681 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     1741 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     1801 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     1861 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     1921 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     1981 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     2041 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     2101 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     2161 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     2221 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     2281 ttttgctggc cttttgctca catgtcctgc aggcagctgc gcgctcgctc gctcactgag
     2341 gccgcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc gagcgcgcag
     2401 agagggagtg gccaactcca tcactagggg ttcctgcggc ctctagactc gaattgggta
     2461 ccgagggcct atttcccatg attccttcat atttgcatat acgatacaag gctgttagag
     2521 agataattgg aattaattcg actgtaaaca caaagatatt agtacaaaat acgtgacgta
     2581 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
     2641 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2701 gacgaaacac cgagtcccca gcaggatcac atgttttaga gctagaaata gcaagttaaa
     2761 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttccgcg
     2821 gtggagagct ggagctcccc tatgtgatcc tgctggggac tatgtcccct aggttcctgg
     2881 ggagggagga cgtcggggcc agccaggctt accccccctg ccgctgagac ttctgcgctg
     2941 gtgcaccgcg cctcttcgcg gtctccctgt ccttgcagag aaccggtcag gccaccatgc
     3001 agcgcgtgaa catgatcatg gcagaatcac caggcctcat caccatctgc cttttaggat
     3061 atctactcag tgctgaatgt acagtttttc ttgatcatga aaacgccaac aaaattctga
     3121 atcggccaaa gaggtataat tcaggtaaat tggaagagtt tgttcaaggg aaccttgaga
     3181 gagaatgtat ggaagaaaag tgtagttttg aagaagcacg agaagttttt gaaaacactg
     3241 aaagaacaac tgaattttgg aagcagtatg ttgatggaga tcagtgtgag tccaatccat
     3301 gtttaaatgg cggcagttgc aaggatgaca ttaattccta tgaatgttgg tgtccctttg
     3361 gatttgaagg aaagaactgt gaattagatg taacatgtaa cattaagaat ggcagatgcg
     3421 agcagttttg taaaaatagt gctgataaca aggtggtttg ctcctgtact gagggatatc
     3481 gacttgcaga aaaccagaag tcctgtgaac cagcagtgcc atttccatgt ggaagagttt
     3541 ctgtttcaca aacttctaag ctcacccgtg ctgagactgt ttttcctgat gtggactatg
     3601 taaattctac tgaagctgaa accattttgg ataacatcac tcaaagcacc cagtcattta
     3661 atgacttcac tcgggttgtt ggtggagaag atgccaaacc aggtcaattc ccttggcagg
     3721 ttgttttgaa tggtaaagtt gatgcattct gtggaggctc tatcgttaat gaaaaatgga
     3781 ttgtaactgc tgcccactgt gttgaaactg gtgttaaaat tacagttgtc gcaggtgaac
     3841 ataatattga ggagacagaa catacagagc aaaagcgaaa tgtgattcga attattcctc
     3901 accacaacta caatgcagct attaataagt acaaccatga cattgccctt ctggaactgg
     3961 acgaaccctt agtgctaaac agctacgtta cacctatttg cattgctgac aaggaataca
     4021 cgaacatctt cctcaaattt ggatctggct atgtaagtgg ctggggaaga gtcttccaca
     4081 aagggagatc agctttagtt cttcagtacc ttagagttcc acttgttgac cgagccacat
     4141 gtcttcgatc tacaaagttc accatctata acaacatgtt ctgtgctggc ttccatgaag
     4201 gaggtagaga ttcatgtcaa ggagatagtg ggggacccca tgttactgaa gtggaaggga
     4261 ccagtttctt aactggaatt attagctggg gtgaagagtg tgcaatgaaa ggcaaatatg
     4321 gaatatatac caaggtatcc cggtatgtca actggattaa ggaaaaaaca aagctcactt
     4381 aatgaaagat ggatttccaa ggttaattca ttggaattga aaattaacag ggcctctcac
     4441 taactaatca ctttcccatc ttttgttaga tttgaatata tacattctat gatcattgct
     4501 ttttctcttt acaggggaga atttcatatt ttacctgagc aaattgatta gaaaatggaa
     4561 ccactagagg aatataatgt gttaggaaat tacagtcatt tctaagggcc cagcccttga
     4621 caaaattgtg aagttaaatt ctccactctg tccatcagat actatggttc tccactatgg
     4681 caactaactc actcaatttt ccctccttag cagcattcca tcttcccaat cttctttgct
     4741 tctccaacca aaacatcaat gtttattagt tctgtataca gtacaggatt ctttggtcta
     4801 ctctatcaca aggccagtat cacactcatg aagaaagaac acaggagtag ctgagaggct
     4861 aaaactcatc aaaaacacta ctccttttcc tctaccctat tcctcaatct tttacctttt
     4921 ccaaatccca atccccaaat cagtttttct ctttcttact ccctctctcc cttttaccct
     4981 ccatggtcgt taaaggagag atggggagca tcattctgtt atacttctgt acacagttat
     5041 acatgtctat caaacccaga cttgcttccg tagtggagac ttgcttttca gaacataggg
     5101 atgaagtaag gtgcctgaaa agtttggggg aaaagtttct ttcagagagt taagttattt
     5161 tatatatata atatatatat aaaatatata atatacaata taaatatata gtgtgtgtgt
     5221 atgcgtgtgt gtagacacac acgcatacac acatataatg gaagcaataa gccattctaa
     5281 gagcttgtat ggttatggag gtctgactag gcatgatttc acgaaggcaa gattggcata
     5341 tcattgtaac taaaaaagct gacattgacc cagacatatt gtactctttc taaaaataat
     5401 aataataatg ctaacagaaa gaagagaacc gttcgtttgc aatctacagc tagtagagac
     5461 tttgaggaag aattcaacag tgtgtcttca gcagtgttca gagccaagca agaagttgaa
     5521 gttgcctaga ccagaggaca taagtatcat gtctccttta actagcatac cccgaagtgg
     5581 agaagggtgc agcaggctca aaggcataag tcattccaat cagccaacta agttgtcctt
     5641 ttctggtttc gtgttcacca tggaacattt tgattatagt taatccttct atcttgaatc
     5701 ttctagagag ttgctgacca actgacgtat gtttcccttt gtgaattaat aaactggtgt
     5761 tctggttcat ggatccagac atgataagat acattgatga gtttggacaa accacaacta
     5821 gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa
     5881 ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg
     5941 ttcaggggga ggtgtgggag gttttttagt cgacctatgt gatcctgctg gggactggta
     6001 cccaattcgc gcagaccggt cgccaccatg gtgagcaagg gcgaggagct gttcaccggg
     6061 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc
     6121 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc
     6181 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc
     6241 ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa
     6301 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc
     6361 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc
     6421 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc
     6481 tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac
     6541 atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac
     6601 ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct gagcaaagac
     6661 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
     6721 ctcggcatgg acgagctgta caagtaaagc ggccgcgcag gaacccctag tgatggagtt
     6781 ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg
     6841 acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     6901 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     6961 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     7021 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     7081 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     7141 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gg
//
