LOCUS       RNA-Protein_Land       10318 bp DNA     circular SYN 26-JUL-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Zack
  TITLE     Direct Submission
  JOURNAL   Exported Jul 26, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(404..1195,1196..1264))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1:  404 .. 1195 / #ccffcc
                        2: 1196 .. 1264 / #ccffcc / signal sequence
                     Cleavage site after base 1195"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(1265..1369)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     promoter        1632..1981
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    1640..1658
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1676..1694
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1712..1730
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1748..1766
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1784..1802
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1820..1838
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    1856..1874
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    1983..2030
                     /label=Bxb1 attP Integration Site
                     /note="color: #ff6600"
     misc_feature    1983..2005
                     /label=P
                     /note="color: #ff9900"
     misc_feature    2006..2007
                     /label=P & P'
                     /note="color: #ff9900"
     misc_feature    2008..2030
                     /label=P'
                     /note="color: #ff9900"
     misc_feature    2031..2057
                     /label=+27 part of promoter
                     /note="Encodes the first 27nt of U6 RNA which includes a 
                     hairpin right at the beginning. This directs methylation of
                     the 5′-gamma-phosphate and stabilizes the transcript. 
                     Reference: Effective expression of smallinterfering RNA in 
                     humancells, 2002, Paul et al. 
                     "
                     /note="color: #ffffff"
     misc_feature    2039..2057
                     /label=hairpin
                     /note="color: #ffffff"
     misc_feature    2057^2058
                     /label=transcription start site
                     /note="I'm like 90% sure..... this is 23nt after a 
                     ""tatata"" sequence (which is likely the TATA box?). A 
                     brief lit search says U6 transcription start site is +1 
                     position 23nt after the TATA box"
                     /note="color: #ff9900"
     promoter        complement(2066..2306)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
                     /note="color: #ffffff; direction: LEFT"
     regulatory      2331..2340
                     /label=Kozak
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             2337..3047
                     /codon_start=1
                     /product="enhanced monomeric blue fluorescent protein 
                     (Subach et al., 2011)"
                     /label=mTagBFP2
                     /note="mammalian codon-optimized"
                     /note="color: #3366ff"
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     misc_feature    3022..3038
                     /label=Feature 50
                     /note="color: #a6acb3"
     CDS             3057..3113
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 3110"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3123..4325
                     /codon_start=1
                     /label=iCasp9
                     /note="color: #99cc00"
                     /translation="LEGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKVDSSRDRN
                     KPFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVE
                     LLKLESGGGSGVDGFGDVGALESLRGNADLAYILSMEPCGHCLIINNVNFCRESGLRTR
                     TGSNIDCEKLRRRFSSLHFMVEVKGDLTAKKMVLALLELARQDHGALDCCVVVILSHGC
                     QASHLQFPGAVYGTDGCPVSVEKIVNIFNGTSCPSLGGKPKLFFIQACGGEQKDHGFEV
                     ASTSPEDESPGSNPEPDATPFQEGLRTFDQLDAISSLPTPSDIFVSYSTFPGFVSWRDP
                     KSGSWYVETLDDIFEQWAHSEDLQSLLLRVANAVSVKGIYKQMPGCFNFLRKKLFFKTS
                     VD"
     CDS             3129..3449
                     /product="F36V mutant of FK506-binding protein FKBP12"
                     /label=DmrB
                     /note="binds synthetic ligands that do not bind wild-type 
                     FKBP"
                     /note="color: #993366; direction: RIGHT"
     CDS             complement(3801..3812)
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /note="color: #cc99b2; direction: LEFT
                     Cleavage site after base 3800"
     CDS             4326..4352
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="color: #cc99b2"
                     /translation="YPYDVPDYA"
     misc_feature    4374..4900
                     /label=rb glob terminator
                     /note="color: #a6acb3"
     polyA_signal    4519..4574
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
                     /note="color: #a6acb3"
     enhancer        4908..5211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        5212..5415
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    5520..5544
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
                     /note="color: #31849b; direction: RIGHT"
     CDS             5547..6254
                     /codon_start=1
                     /product="reverse tetracycline transactivator 3"
                     /label=rtTA3
                     /note="activates expression from Tet-responsive promoters 
                     in the presence of doxycycline"
                     /note="color: #993366"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPG"
     polyA_signal    6281..6505
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     protein_bind    6789..6836
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
                     /note="color: #31849b; direction: RIGHT"
     CDS             6844..7866
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /note="color: #ccffcc"
                     /translation="KKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGY
                     VLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPE
                     TELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQT
                     VMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFG
                     DSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGN
                     FDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE
                     "
     misc_feature    join(7955..8529,8530..8532,8533..8541)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 7955 .. 8529 / #a6acb3
                        2: 8530 .. 8532 / #a6acb3 / ATG
                        3: 8533 .. 8541 / #a6acb3"
     CDS             join(8542..8544,8545..8547,8548..9258)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1: 8542 .. 8544 / #05fc14
                        2: 8545 .. 8547 / #05fc14 / 1a
                        3: 8548 .. 9258 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     polyA_signal    9391..9512
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(9561..9577)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     protein_bind    9585..9601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(9609..9615,9616..9633,9634..9639))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 9609 .. 9615 / #ffffff / -10
                        2: 9616 .. 9633 / #ffffff
                        3: 9634 .. 9639 / #ffffff / -35"
     protein_bind    9654..9675
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     rep_origin      complement(join(9963..10318,1..233))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
       61 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
      121 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
      181 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
      241 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
      301 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
      361 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
      421 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
      481 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
      541 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
      601 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
      661 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
      721 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
      781 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
      841 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
      901 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
      961 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     1021 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     1081 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     1141 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     1201 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     1261 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     1321 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     1381 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     1441 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     1501 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     1561 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg aaggcccttt
     1621 cgtcttcact cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct
     1681 atcagtgata gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg
     1741 agtttactcc ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga
     1801 gaacgtatct acagtttact ccctatcagt gatagagaac gtatatccag tttactccct
     1861 atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga
     1921 gctcgtttag tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact
     1981 tgggtttgtc tggtcaacca ccgcggtctc agtggtgtac ggtacaaacc tagtatatgt
     2041 gctgccgaag cgagcacggt gtttcgtcct ttccacaaga tatataaagc caagaaatcg
     2101 aaatactttc aagttacggt aagcatatga tagtccattt taaaacataa ttttaaaact
     2161 gcaaactacc caagaaatta ttactttcta cgtcacgtat tttgtactaa tatctttgtg
     2221 tttacagtca aattaattct aattatctct ctaacagcct tgtatcgtat atgcaaatat
     2281 gaaggaatca tgggaaatag gccctcttcc tgcccgacct tggatccggc gccaccatgg
     2341 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     2401 ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     2461 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     2521 tggctactag cttcctctac ggcagcaaga ccttcatcaa ccacacccag ggcatccccg
     2581 acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     2641 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     2701 acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag aagaaaacac
     2761 tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg gaaggcagaa
     2821 acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacgcc aagaccacat
     2881 atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat gtggactaca
     2941 gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac gaggtggcag
     3001 tggccagata ctgcgacctc cctagcaaac tggggcacaa gcttaatggg agcggagcta
     3061 ctaacttcag cctgctgaag caggctggag acgtggagga gaaccctgga ccttccgcca
     3121 ccctcgaggg agtgcaggtg gagactatct ccccaggaga cgggcgcacc ttccccaagc
     3181 gcggccagac ctgcgtggtg cactacaccg ggatgcttga agatggaaag aaagttgatt
     3241 cctcccggga cagaaacaag ccctttaagt ttatgctagg caagcaggag gtgatccgag
     3301 gctgggaaga aggggttgcc cagatgagtg tgggtcagag agccaaactg actatatctc
     3361 cagattatgc ctatggtgcc actgggcacc caggcatcat cccaccacat gccactctcg
     3421 tcttcgatgt ggagcttcta aaactggaat ctggcggtgg atccggagtc gacggatttg
     3481 gtgatgtcgg tgctcttgag agtttgaggg gaaatgcaga tttggcttac atcctgagca
     3541 tggagccctg tggccactgc ctcattatca acaatgtgaa cttctgccgt gagtccgggc
     3601 tccgcacccg cactggctcc aacatcgact gtgagaagtt gcggcgtcgc ttctcctcgc
     3661 tgcatttcat ggtggaggtg aagggcgacc tgactgccaa gaaaatggtg ctggctttgc
     3721 tggagctggc gcggcaggac cacggtgctc tggactgctg cgtggtggtc attctctctc
     3781 acggctgtca ggccagccac ctgcagttcc caggggctgt ctacggcaca gatggatgcc
     3841 ctgtgtcggt cgagaagatt gtgaacatct tcaatgggac cagctgcccc agcctgggag
     3901 ggaagcccaa gctctttttc atccaggcct gtggtgggga gcagaaagac catgggtttg
     3961 aggtggcctc cacttcccct gaagacgagt cccctggcag taaccccgag ccagatgcca
     4021 ccccgttcca ggaaggtttg aggaccttcg accagctgga cgccatatct agtttgccca
     4081 cacccagtga catctttgtg tcctactcta ctttcccagg ttttgtttcc tggagggacc
     4141 ccaagagtgg ctcctggtac gttgagaccc tggacgacat ctttgagcag tgggctcact
     4201 ctgaagacct gcagtccctc ctgcttaggg tcgctaatgc tgtttcggtg aaagggattt
     4261 ataaacagat gcctggttgc tttaatttcc tccggaaaaa acttttcttt aaaacatcag
     4321 tcgactatcc gtacgacgta ccagactacg cactcgacta gtaaacgcgt gaattcactc
     4381 ctcaggtgca ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca
     4441 aataccactg agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg
     4501 agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt
     4561 ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg
     4621 agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg aacaaaggtt
     4681 ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc ttattccata
     4741 gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta tttttttctt
     4801 taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac
     4861 tactcccagt catagctgtc cctcttctct tatggagatc taaaccgcgt tacataactt
     4921 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     4981 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     5041 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     5101 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     5161 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     5221 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     5281 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     5341 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     5401 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
     5461 tttgacctcc atagaagaca ccgactctag tccagtgtgg tggaattctg cagatatcaa
     5521 caagtttgta caaaaaagtt ggcaccatgt ctagactgga caagagcaaa gtcataaacg
     5581 gagctctgga attactcaat ggtgtcggta tcgaaggcct gacgacaagg aaactcgctc
     5641 aaaagctggg agttgagcag cctaccctgt actggcacgt gaagaacaag cgggccctgc
     5701 tcgatgccct gccaatcgag atgctggaca ggcatcatac ccacttctgc cccctggaag
     5761 gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc ataccgctgt gctctcctct
     5821 cacatcgcga cggggctaaa gtgcatctcg gcacccgccc aacagagaaa cagtacgaaa
     5881 ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt ctccctggag aacgcactgt
     5941 acgctctgtc cgccgtgggc cactttacac tgggctgcgt attggaggaa caggagcatc
     6001 aagtagcaaa agaggaaaga gagacaccta ccaccgattc tatgccccca cttctgagac
     6061 aagcaattga gctgttcgac cggcagggag ccgaacctgc cttccttttc ggcctggaac
     6121 taatcatatg tggcctggag aaacagctaa agtgcgaaag cggcgggccg accgacgccc
     6181 ttgacgattt tgacttagac atgctcccag ccgatgccct tgacgatttt gaccttgaca
     6241 tgctccccgg gtaacgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6301 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6361 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6421 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6481 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
     6541 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     6601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     6661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     6721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     6781 cgtacctaga agttcctatt ccgaagttcc tattctctag aaagtatagg aacttccttg
     6841 gccaaaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     6901 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     6961 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     7021 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     7081 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     7141 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     7201 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
     7261 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
     7321 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
     7381 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
     7441 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
     7501 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
     7561 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
     7621 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
     7681 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
     7741 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
     7801 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
     7861 gaatagatgc atcgcggccg ctcattgctc gaggaattcg ctagcgggat ccaccggatc
     7921 tagataactg atcactgtgg aatcagagag actgcccctc tccctctccc ccccctaacg
     7981 ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca
     8041 ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga
     8101 gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga
     8161 aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca
     8221 ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag
     8281 atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa
     8341 gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac
     8401 cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga
     8461 ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt tgaaaaacac
     8521 gatgataata tggccacaac catggtgagc aagggcgagg agctgttcac cggggtggtg
     8581 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     8641 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     8701 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     8761 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     8821 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     8881 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     8941 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     9001 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     9061 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     9121 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
     9181 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     9241 atggacgagc tgtacaagta gcacgtacta cgagatttcg attccaccgc cgccttctat
     9301 gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct ccagcgcggg
     9361 gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta taatggttac
     9421 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt
     9481 tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc gacctctagc
     9541 tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     9601 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     9661 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9721 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     9781 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     9841 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     9901 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     9961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
    10021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
    10081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
    10141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
    10201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
    10261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagcca
//
