LOCUS       Myc-TurboID-CRAF       11411 bp DNA     circular SYN 27-AUG-2024
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11411)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 11411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 27, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLJM1-EGFP
FEATURES             Location/Qualifiers
     source          1..11411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        290..493
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     497..548
                     /label=pCDNA5 --> pLJM1_fwd (NheI overlap)
                     /note="color: black; sequence: 
                     ttagtgaaccgtcagatccgctagcgctaccggtAAGCTTgccgccaccatg; 
                     added: 2023-03-21"
     misc_feature    537..549
                     /label=kozak
                     /note="color: #a6acb3"
     CDS             549..578
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /note="color: #cc99b2"
                     /translation="EQKLISEEDL"
     CDS             585..1541
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /note="color: #993366"
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             1548..1577
                     /codon_start=1
                     /product="Generic linker"
                     /label=linker
                     /note="color: #ffcc00"
                     /translation="GGSGSGSGSG"
     CDS             1578..3521
                     /codon_start=1
                     /label=CRaf
                     /note="color: #ff9900"
                     /translation="MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDD
                     GKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHE
                     HKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGF
                     RCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVS
                     RMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAI
                     RSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSY
                     YWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRK
                     TRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDY
                     LHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVI
                     RMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKN
                     CPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDIN
                     ACTLTTSPRLPVF"
     primer_bind     complement(3525..3567)
                     /label=pCDNA5 --> pLJM1_rev (EcoRI overlap)
                     /note="color: black; sequence: 
                     ccatttgtctcgaggtcgagaattcGGGCCCTCTAGACTCGAG; added: 
                     2023-03-21"
     primer_bind     3548..3567
                     /label=cPPT_fwd
                     /note="color: black; sequence: ctcgacctcgagacaaatgg; added:
                     2023-03-21"
     misc_feature    3584..3701
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        3750..4260
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     4254..4292
                     /label=EGFP_fwd (hPGK overlap)
                     /note="color: black; sequence: 
                     tccccagggggatccaccggagccaccatggtgagcaag; added: 2023-03-21"
     primer_bind     complement(4254..4274)
                     /label=hPGK_rev
                     /note="color: black; sequence: tccggtggatccccctgggga; 
                     added: 2023-03-21"
     regulatory      4275..4284
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(4281..4283,4284..4286,4287..4997)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="This feature has 3 segments:
                        1: 4281 .. 4283 / #05fc14
                        2: 4284 .. 4286 / #05fc14 / 1a
                        3: 4287 .. 4997 / #05fc14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4979..5021)
                     /label=EGFP_rev (IRES overlap)
                     /note="color: black; sequence: 
                     GCAGTCTCTCTGATTCCACAGtcacttgtacagctcgtccatg; added: 
                     2023-03-22"
     primer_bind     5001..5021
                     /label=IRES_fwd
                     /note="color: black; sequence: CTGTGGAATCAGAGAGACTGC; 
                     added: 2023-03-22"
     misc_feature    join(5021..5595,5596..5598,5599..5607)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
                     /note="This feature has 3 segments:
                        1: 5021 .. 5595 / #a6acb3
                        2: 5596 .. 5598 / #a6acb3 / ATG
                        3: 5599 .. 5607 / #a6acb3"
     primer_bind     complement(5586..5629)
                     /label=IRES_rev (blast overlap)
                     /note="color: black; sequence: 
                     cttgagacaaaggcttggcCATGGTTGTGGCCATATTATCATCG; added: 
                     2023-03-22"
     CDS             5611..6006
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /note="color: #ccffcc"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     primer_bind     5611..5629
                     /label=Blast_fwd
                     /note="color: black; sequence: gccaagcctttgtctcaag; added: 
                     2023-03-22"
     primer_bind     complement(5988..6036)
                     /label=Blast_rev (KpnI overlap)
                     /note="color: black; sequence: 
                     gccttgtaagtcattggtcttaaaggtaccctagccctcccacacataa; added: 
                     2023-03-22"
     LTR             6078..6311
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     polyA_signal    6383..6504
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6544..6679
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     promoter        complement(6700..6718)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(6728..6744)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     rep_origin      6886..7341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        7367..7471
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(7472..7540,7541..8332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7472 .. 7540 / #ccffcc / signal sequence
                        2: 7541 .. 8332 / #ccffcc
                     Cleavage site after base 7540"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8503..9091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    9379..9400
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(9415..9420,9421..9438,9439..9445)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 9415 .. 9420 / #ffffff / -35
                        2: 9421 .. 9438 / #ffffff
                        3: 9439 .. 9445 / #ffffff / -10"
     protein_bind    9453..9469
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     9477..9493
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        9514..9532
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     promoter        9560..9786
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             9787..9967
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10014..10139
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10632..10865
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     CDS             11050..11094
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
     primer_bind     join(11392..11411,1)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
                     /note="color: black; sequence: GAGGGCCTATTTCCCATGATT"
ORIGIN
        1 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattggaa ttaatttgac
       61 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
      121 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgccaagta cgccccctat
      181 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      241 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      301 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      361 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      421 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      481 tataagcaga gctggtttag tgaaccgtca gatccgctag cgctaccggt aagcttgccg
      541 ccaccatgga acaaaaactc atctcagaag aggatctcgg tggaaaagac aatactgtgc
      601 ctctgaagct gatcgctctc ctggctaatg gcgagttcca tagtggcgaa cagctgggag
      661 aaaccctggg catgtccagg gccgctatca acaagcacat tcagactctg cgcgactggg
      721 gcgtggacgt gttcaccgtg cccggaaagg gctactctct gcccgagcct atcccgctgc
      781 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
      841 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
      901 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
      961 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     1021 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     1081 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     1141 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     1201 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     1261 aagcagggat taacctggac aggaatactc tggccgctac gctgatccga gagctgcggg
     1321 cagccctgga actgttcgag caggaaggcc tggctccata tctgccacgg tgggagaagc
     1381 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     1441 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     1501 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa gctgcagggt ggcagcggta
     1561 gtggatctgg ctcaggaatg gagcacatac agggagcttg gaagacgatc agcaatggtt
     1621 ttggattcaa agatgccgtg tttgatggct ccagctgcat ctctcctaca atagttcagc
     1681 agtttggcta tcagcgccgg gcatcagatg atggcaaact cacagatcct tctaagacaa
     1741 gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat gtgcgaaatg
     1801 gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg caaccagagt
     1861 gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc ttagattgga
     1921 atactgatgc tgcgtctttg attggagaag aacttcaagt agatttcctg gatcatgttc
     1981 ccctcacaac acacaacttt gctcggaaga cgttcctgaa gcttgccttc tgtgacatct
     2041 gtcagaaatt cctgctcaat ggatttcgat gtcagacttg tggctacaaa tttcatgagc
     2101 actgtagcac caaagtacct actatgtgtg tggactggag taacatcaga caactcttat
     2161 tgtttccaaa ttccactatt ggtgatagtg gagtcccagc actaccttct ttgactatgc
     2221 gtcgtatgcg agagtctgtt tccaggatgc ctgttagttc tcagcacaga tattctacac
     2281 ctcacgcctt cacctttaac acctccagtc cctcatctga aggttccctc tcccagaggc
     2341 agaggtcgac atccacacct aatgtccaca tggtcagcac caccctgcct gtggacagca
     2401 ggatgattga ggatgcaatt cgaagtcaca gcgaatcagc ctcaccttca gccctgtcca
     2461 gtagccccaa caatctgagc ccaacaggct ggtcacagcc gaaaaccccc gtgccagcac
     2521 aaagagagcg ggcaccagta tctgggaccc aggagaaaaa caaaattagg cctcgtggac
     2581 agagagattc aagctattat tgggaaatag aagccagtga agtgatgctg tccactcgga
     2641 ttgggtcagg ctcttttgga actgtttata agggtaaatg gcacggagat gttgcagtaa
     2701 agatcctaaa ggttgtcgac ccaaccccag agcaattcca ggccttcagg aatgaggtgg
     2761 ctgttctgcg caaaacacgg catgtgaaca ttctgctttt catggggtac atgacaaagg
     2821 acaacctggc aattgtgacc cagtggtgcg agggcagcag cctctacaaa cacctgcatg
     2881 tccaggagac caagtttcag atgttccagc taattgacat tgcccggcag acggctcagg
     2941 gaatggacta tttgcatgca aagaacatca tccatagaga catgaaatcc aacaatatat
     3001 ttctccatga aggcttaaca gtgaaaattg gagattttgg tttggcaaca gtaaagtcac
     3061 gctggagtgg ttctcagcag gttgaacaac ctactggctc tgtcctctgg atggccccag
     3121 aggtgatccg aatgcaggat aacaacccat tcagtttcca gtcggatgtc tactcctatg
     3181 gcatcgtatt gtatgaactg atgacggggg agcttcctta ttctcacatc aacaaccgag
     3241 atcagatcat cttcatggtg ggccgaggat atgcctcccc agatcttagt aagctatata
     3301 agaactgccc caaagcaatg aagaggctgg tagctgactg tgtgaagaaa gtaaaggaag
     3361 agaggcctct ttttccccag atcctgtctt ccattgagct gctccaacac tctctaccga
     3421 agatcaaccg gagcgcttcc gagccatcct tgcatcgggc agcccacact gaggatatca
     3481 atgcttgcac gctgaccacg tccccgaggc tgcctgtctt ctgactcgag tctagagggc
     3541 ccgaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     3601 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3661 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     3721 gcagagatcc actttggccg cggctcgagg gggttggggt tgcgcctttt ccaaggcagc
     3781 cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc
     3841 gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct
     3901 acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg
     3961 cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca
     4021 gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc
     4081 ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg
     4141 tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct
     4201 ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag
     4261 ggggatccac cggagccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
     4321 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     4381 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
     4441 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     4501 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     4561 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     4621 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     4681 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     4741 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     4801 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     4861 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     4921 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     4981 tggacgagct gtacaagtga ctgtggaatc agagagactg cccctctccc tctccccccc
     5041 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     5101 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     5161 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     5221 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     5281 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     5341 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     5401 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     5461 aggtacccca ttgtatggga tctgatctgg ggcctcggtg cacatgcttt acatgtgttt
     5521 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     5581 aaacacgatg ataatatggc cacaaccatg gccaagcctt tgtctcaaga agaatccacc
     5641 ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc
     5701 gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt
     5761 actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc
     5821 aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga
     5881 cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt
     5941 gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag
     6001 ggctagggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     6061 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     6121 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     6181 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     6241 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     6301 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     6361 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     6421 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     6481 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     6541 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     6601 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     6661 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     6721 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     6781 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     6841 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     6901 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6961 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     7021 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     7081 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     7141 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     7201 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     7261 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     7321 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     7381 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     7441 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     7501 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     7561 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     7621 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     7681 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     7741 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     7801 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     7861 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     7921 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     7981 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     8041 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     8101 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     8161 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     8221 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     8281 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     8341 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     8401 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     8461 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     8521 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     8581 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     8641 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     8701 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     8761 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     8821 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     8881 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     8941 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     9001 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     9061 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     9121 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     9181 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     9241 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     9301 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     9361 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     9421 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     9481 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     9541 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     9601 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     9661 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     9721 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     9781 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     9841 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     9901 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     9961 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10021 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10081 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10141 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10201 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10261 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10321 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10381 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10441 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
    10501 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
    10561 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
    10621 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
    10681 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
    10741 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
    10801 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
    10861 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
    10921 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10981 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    11041 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    11101 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    11161 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    11221 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    11281 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    11341 tctcgacggt atcgatcacg agactagcct cgagcggccg cccccttcac cgagggccta
    11401 tttcccatga t
//
