LOCUS       226172-attachmen        9973 bp DNA     circular SYN 04-NOV-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 4, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      928..1516
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             1699..1791
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.6"
                     /note="/other=CDS"
                     /translation="APNTQTASPRALADSLMQLARQVSRLESGQ"
     protein_bind    1804..1825
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1840..1870
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    1878..1894
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        1939..1957
                     /label=T3 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    2214..2247
                     /label=loxP
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             2268..2307
                     /codon_start=1
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
                     /translation="AGTTGFPTGKRAV"
     protein_bind    2320..2341
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2356..2386
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    2394..2410
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     polyA_signal    complement(2795..2850)
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     CDS             complement(3012..3401)
                     /codon_start=1
                     /label=bsd
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGVN
                     VYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIKA
                     IVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    complement(3415..3877)
                     /label=IRES
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=misc_feature"
     misc_feature    3993..4025
                     /label=MCS (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=30.6"
                     /note="/other=misc_feature"
     CDS             complement(4042..4104)
                     /codon_start=1
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.2"
                     /note="/match_length=58.3"
                     /note="/other=CDS"
                     /translation="EFTPQVQAAYQKVVAGVANWL"
     protein_bind    4136..4160
                     /label=attB2
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(4180..6915)
                     /codon_start=1
                     /label=Proser1
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=99.9"
                     /note="/other=CDS"
                     /translation="DKKSFETVLDEIRKAVLTEYKLKAIEYVHGYFSSEQVVDLLRYFS
                     WAEPQLKAMKALQHKMVAVHPAEVVSILSCFTFSKDKLAALELLASNIVDAQNSRPIED
                     LFRINMSEKKRCKRVLEQASKAGCKAPHAMISSCGTFPGNPYPKGKPSRINGIFPGTPL
                     KKDGEEITNEGKGIAARILGPSKPPPSTYNPHKPVPYPIPPCRPHATIAPSAYNNAGLV
                     PLANVIAPGVPPPPPYTPNPAGTDNEDLSSQSKPTQSQTFSTPASQLFSPHGSSNPSTP
                     AATPVPAVSPAKAVNHPSVSAAATGAGMSATNTALAVFPTPQPNTPNPTVIRTPSVPAT
                     PVTSTHSTTPTPVPSVFSGLVPLPGLSATPTLPTQASSISRVTLASSETFASTCAPFSG
                     HSSASSTAGSASNPITAPLSSVFAGLPLPFPPASHSIATPTPSVIASAAGPHGVNSPLL
                     SALKGFLTSNDTHLINSSALPSAVTSGLASLSSLPNRNSDSPASATNKCYPPAAVPAAQ
                     RSSTPGLAMFPGLQSPVASATSVAPTLPAQSPLATPSTAVPVSCGSSGSLLHGPHAGGI
                     SSAPAAATMPVMIKSEPTSPPPSAFKGPAHPGTPVRGTLGLSGALGRGYPTTSVPISVS
                     TCLNPALSGLSSLSTPLAGSMSLPPHASSTPIAPVFTALPPFTSLTNSFPLPGSPSLNP
                     AVSLAGSSTTTTSAAVHPSSATVRSVLPTSNASPAAFPLNLSTAVPSLFAVTQGPLPTS
                     NPSYPGFPVSSAPSGAPTLPSFPGLQAPSTVAVTPLPVAASAPSPAPVLPGFASAFSSN
                     FNSALVAQAGLSSGLQAAGSSVFPGLLSLPGIPGFSQAPPQSSLQELQHSAAAQSALLQ
                     QVHSASALESYPAQADGFPSYPSTPGTPFSLQTGLSQSGWQ"
     misc_feature    complement(4702..4722)
                     /label=Proser1 C-term 4 Fw
                     /label=nonstandard type: Site
                     /note="/site_type=modification"
     misc_feature    complement(5341..5361)
                     /label=Proser1 C-term 3 Fw
                     /label=nonstandard type: Site
                     /note="/site_type=modification"
     misc_feature    complement(5929..5949)
                     /label=Proser1 C-term 2 Fw
                     /label=nonstandard type: Site
                     /note="/site_type=modification"
     misc_feature    complement(6517..6537)
                     /label=Proser1 C-term 1 Fw
                     /label=nonstandard type: Site
                     /note="/site_type=modification"
     misc_feature    6916..6936
                     /label=Proser1 C-term Rv
                     /label=nonstandard type: Site
                     /note="/site_type=modification"
     CDS             complement(6931..6951)
                     /codon_start=1
                     /label=FLAG (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=87.5"
                     /note="/other=CDS"
                     /translation="YKDDDDK"
     CDS             complement(6955..6975)
                     /codon_start=1
                     /label=FLAG (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=87.5"
                     /note="/other=CDS"
                     /translation="YKDDDDK"
     protein_bind    complement(7002..7026)
                     /label=attB1
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     intron          complement(7090..8106)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     promoter        complement(8107..8383)
                     /label=chicken beta-actin promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.6"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        complement(8385..8764)
                     /label=CMV enhancer
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=enhancer"
     promoter        complement(9101..9119)
                     /label=T7 promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             9122..9279
                     /codon_start=1
                     /label=lacZ-alpha (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=90.8"
                     /note="/other=CDS"
                     /translation="IHWPSFYNVVTGKTLALPNLIALQHIPLSPAGVIAKRPAPIALPN
                     SCAA*MA"
     rep_origin      9284..9712
                     /label=f1 ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     promoter        9765..9869
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     CDS             join(9870..9973,1..757)
                     /codon_start=1
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
       61 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
      121 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
      181 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
      241 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
      301 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
      361 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
      421 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
      481 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
      541 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
      601 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
      661 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
      721 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
      781 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      841 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      901 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      961 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     1021 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     1081 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     1141 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     1201 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     1261 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1321 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1381 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1441 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1501 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1561 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1621 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1681 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     1741 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     1801 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     1861 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     1921 tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa caaaagctgg agctctttaa
     1981 ccctagaaag ataatcatat tgtgacgtac gttaaagata atcatgcgta aaattgacgc
     2041 atgtgtttta tcggtctgta tatcgaggtt tatttattaa tttgaataga tattaagttt
     2101 tattatattt acacttacat actaataata aattcaacaa acaatttatt tatgtttatt
     2161 tatttattaa aaaaaaacaa aaactcaaaa tttcttctat aaagtaacaa aacataactt
     2221 cgtatagcat acattatacg aagttatctc gacgcggccg cggatccgct ggcacgacag
     2281 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
     2341 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
     2401 cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca
     2461 ggtcgaggga tctccataag agaagaggga cagctatgac tgggagtagt caggagagga
     2521 ggaaaaatct ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata
     2581 acacaaaaca aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg
     2641 aatggacagc agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca
     2701 tggcagccag catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt
     2761 ttaaatgatt tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca
     2821 ctattgcaat gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca
     2881 tgatgtcccc ataatttttg gcagagggaa aaagatcgac ccccctcgga cccgtcgggc
     2941 cgcgtcggac aaagcaagta aaacctctac agatgtgata tggctgatta tgatcattac
     3001 ttatctagtt aaccctccca cacataacca gagggcagca attcacgaat cccaactgcc
     3061 gtcggctgtc catcactgtc cttcactatg gctttgatcc caggatgcag atcgagaagc
     3121 acctgtcggc accgtccgca ggggctcaag atgcccctgt tctcatttcc gatcgcgacg
     3181 atacaagtca ggttgccagc tgccgcagca gcagcagtgc ccagcaccac gagttctgca
     3241 caaggtcccc cagtaaaatg atatacattg acaccagtga agatgcggcc gtcgctagag
     3301 agagctgcgc tggcgacgct gtagtcttca gagatgggga tgctgttgat tgtagccgtt
     3361 gctctttcaa tgagggtgga ttcttcttga gacaaaggca tggttgtggc catattatca
     3421 tcgtgttttt caaaggaaaa ccacgtcccc gtggttcggg gggcctagac gtttttttaa
     3481 cctcgactaa acacatgtaa agcatgtgca ccgaggcccc agatcagatc ccatacaatg
     3541 gggtaccttc tgggcatcct tcagcccctt gttgaatacg cttgaggaga gccatttgac
     3601 tctttccaca actatccaac tcacaacgtg gcactggggt tgtgccgcct ttgcaggtgt
     3661 atcttataca cgtggctttt ggccgcagag gcacctgtcg ccaggtgggg ggttccgctg
     3721 cctgcaaagg gtcgctacag acgttgtttg tcttcaagaa gcttccagag gaactgcttc
     3781 cttcacgaca ttcaacagac cttgcattcc tttggcgaga ggggaaagac ccctaggaat
     3841 gctcgtcaag aagacagggc caggtttccg ggccctcaca ttgccaaaag acggcaatat
     3901 ggtggaaaat aacatataga caaacgcaca ccggccttat tccaagcggc ttcggccagt
     3961 aacgttaggg ggggagggag aggggggggg gcggaattcg atatcaagct tatcgatacc
     4021 gtcgatctca gtggtatttg tgagccagtt ggccacacca gccaccacct tctgataggc
     4081 agcctgcacc tgaggagtga attcgatatc aagcttatcg agcggcctcg aatcaaccac
     4141 tttgtacaag aaagctgggt cggcgcgccc accctttcat tgccacccgc tttgggatag
     4201 gcctgtttgc agagaaaatg gtgttcctgg cgtggaggga tagctaggaa agccgtctgc
     4261 ctgggccggg tagctctcca gtgccgatgc tgagtggacc tgctgtaaca aagccgactg
     4321 tgcagctgca ctgtgctgta actcttgcag ggacgactgt ggaggagcct gggaaaaccc
     4381 agggatacct gggagggaca gaaggcctgg aaaaacagag ctacccgcag cttggagtcc
     4441 tgatgacaaa ccggcttgtg ccaccagggc ggagttgaag ttggaactga aggctgaggc
     4501 aaatcccggg aggacaggtg caggcgatgg agccgaggca gccactggca acggggtaac
     4561 tgccacggta gagggcgcct gcagccctgg gaacgagggc agcgtggggg caccgcttgg
     4621 ggcactggag acagggaagc cggggtagga cgggttagag gtgggtagcg ggccctgggt
     4681 gacagcaaag agcgagggta cagcggtgga caggttgagt gggaaggccg caggcgaggc
     4741 attggaggtg ggtagcactg agcgcactgt tgcagagcta gggtgtacag ctgcagatgt
     4801 ggtggtggtc gacgaccccg cgagggatac agcaggattg agggagggac tgcccggtag
     4861 agggaaacta ttagtcaatg aagtgaaagg gggaagggca gtgaagacag gcgcgatggg
     4921 cgtggaggat gcgtgcggcg gaagggacat agaaccagct aacggggtgc tcaggctgga
     4981 gagcccagac agtgcagggt taaggcaagt agacacactg atgggcactg aggtcgtggg
     5041 gtacccacgg cccagggcac ccgacaggcc caaggtacca cgaacaggag tcccggggtg
     5101 agctgggcct ttgaaggccg agggtggggg acttgtgggc tcacttttta tcataacggg
     5161 catagttgct gcagcagggg cagatgagat gccacctgca tggggaccgt gcaaaagtga
     5221 gcctgaggag ccacagctga ctggcactgc cgtggagggg gtagctaacg gagactgtgc
     5281 aggcagcgtt ggggctacag acgtggcact agccacagga gactgcaggc ccggaaacat
     5341 ggccagccct ggggtggaag acctctgtgc agcaggaaca gccgcagggg gatagcactt
     5401 gttagtggcc gaggcaggag aatctgagtt tcgattggga agagaggata gtgaagccag
     5461 tccacttgtg actgcagatg gcaaggcgga ggaattgatt aagtgtgtgt catttgatgt
     5521 aagaaagcct tttaaggcag acagaagggg gctatttaca ccatgtggac ccgcagcact
     5581 ggcaattaca gaaggagtcg gggtggctat gctgtgggat gctggtggga agggcaaagg
     5641 cagacctgcg aagaccgacg agaggggagc tgtgattggg ttactggctg acccagcagt
     5701 agagctggct gaggagtggc cggagaatgg ggcacacgtg gaagcaaatg tctcactgga
     5761 agccagagtg acccttgata tcgagctggc ctgagtgggt agggtggggg tggcagatag
     5821 accgggcagt ggcactaggc cagaaaaaac ggaagggaca ggagtgggtg tggtgctgtg
     5881 ggtggaagta acaggcgttg cgggcacgga gggagtccgg atcacagtcg ggtttggcgt
     5941 gtttggctga ggcgttggga acaccgccag ggcagtgttg gtcgcactca ttccagcccc
     6001 ggtggcagct gctgacacgg atggatggtt tactgccttg gctggagaca cagcaggtac
     6061 tggagtcgca gcaggtgtcg aaggattaga agaaccatga ggagagaaga gctgactcgc
     6121 aggggtggaa aatgtttgac tctgtgtagg ttttgactga ctggaaaggt cttcattgtc
     6181 tgttcctgct ggatttggag tgtacggagg tggaggaggg acgcctggag cgatcacatt
     6241 ggctaatggt accagacccg cattgttgta agcacttggt gcgatagttg catgcggccg
     6301 gcatggagga attgggtagg ggacaggttt gtgtggattg tatgttgaag gaggtggttt
     6361 ggatggcccg agaatccgag ctgctattcc tttgccttca ttggtaattt cttcaccatc
     6421 cttcttcaga ggagttccag ggaaaattcc attgatgcga ctgggtttgc ctttgggata
     6481 ggggtttcct gggaaggttc cacacgaaga aatcatggca tgaggggctt tgcagcccgc
     6541 cttggaagcc tgctccagga ctctcttgca ccgtttcttc tcagacatgt tgattctgaa
     6601 taaatcttca atgggacgag agttctgtgc gtccacaatg tttgaggcca agagctccag
     6661 agcggccagc ttgtccttac taaaggtgaa acagctcaag atgctcacca cttctgctgg
     6721 gtggacggcc accattttat gctggagtgc tttcatggcc ttcagctgtg gctcggccca
     6781 ggagaagtac cttagcagat caaccacctg ttcgctggag aagtacccat gaacatactc
     6841 aattgctttt aatttatatt ccgttaaaac agcctttcga atttcatcca gcaccgtctc
     6901 aaaggacttt ttgtcagatc cgagtccgga cttgtcatcg tcgtccttgt agtccttgtc
     6961 atcgtcgtcc ttgtagtcca tggtgaaggg ggcggccgcg gagcctgctt ttttgtacaa
     7021 acttgttgat tcgaggaatt ctttgccaaa atgatgagac agcacaataa ccagcacgtt
     7081 gcccaggagc tgtaggaaaa agaagaaggc atgaacatgg ttagcagagg ctctagagcc
     7141 gccggtcaca cgccagaagc cgaaccccgc cctgccccgt cccccccgaa ggcagccgtc
     7201 cccccgcgga cagccccgag gctggagagg gagaagggga cggcggcgcg gcgacgcacg
     7261 aaggccctcc ccgcccattt ccttcctgcc ggcgccgcac cgcttcgccc cgcgcccgct
     7321 agagggggtg cggcggcgcc tcccagattt cggctccgca cagatttggg acaaagaaag
     7381 tccctgcgcc ctctcgcacg attaccataa aaggcaatgg ctgcggctcg ccgcgcctcg
     7441 acagccgccg gcgctccggg ggccgccgcg cccctccccc gagccctccc cggcccgagg
     7501 cggccccgcc ccgcccggca cccccacctg ccgccacccc ccgcccggca cggcgagccc
     7561 cgcgccacgc cccgtacgga gccccgcacc cgaagccggg ccgtgctcag caactcgggg
     7621 aggggggtgc agggggggtt gcagcccgac cgacgcgccc acaccccctg ctcacccccc
     7681 cacgcacaca ccccgcacgc agcctttgtt cccctcgcag ccccccccgc accgcggggc
     7741 accgcccccg gccgcgctcc cctcgcgcac actgcggagc gcacaaagcc ccgcgccgcg
     7801 cccgcagcgc tcacagccgc cgggcagcgc ggagccgcac gcggcgctcc ccacgcacac
     7861 acacacgcac gcaccccccg agccgctccc cccgcacaaa gggccctccc ggagcccctc
     7921 aaggctttca cgcagccaca gaaaagaaac aagccgtcat taaaccaagc gctaattaca
     7981 gcccggagga gaagggccgt cccgcccgct cacctgtggg agtaacgcgg tcagtcagag
     8041 ccggggcggg cggcgcgagg cggcggcgga gcggggcacg gggcgaaggc agcgcgcagc
     8101 gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat
     8161 aaaaggaaac tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc
     8221 cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc cccccccgcc
     8281 cccatcgctg cacaaaataa ttaaaaaata aataaataca aaattggggg tggggagggg
     8341 ggggagatgg ggagagtgaa gcagaacgtg gggctcacct cgaccatggt aatagcgatg
     8401 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     8461 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     8521 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     8581 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     8641 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     8701 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     8761 tgtcgagggc cgcgatatct ataacaagaa aatatatata taataagtta tcacgtaagt
     8821 agaacatgaa ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga
     8881 taatcatgcg tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg
     8941 cattgacaag cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag
     9001 cgacggattc gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt
     9061 tacgcagact atctttctag ggttaaggta cccaattcgc cctatagtga gtcgtattac
     9121 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     9181 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     9241 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     9301 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     9361 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     9421 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     9481 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     9541 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     9601 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     9661 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     9721 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     9781 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     9841 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     9901 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     9961 taaaagatgc tga
//
