LOCUS       pDIV643-Addgene      6477 bp    DNA     circular UNA 23-AUG-2024
DEFINITION  .
ACCESSION   BGHA P1
SOURCE      Saccharomyces cerevisiae Saccharomyces cerevisiae Eukaryota; Fungi; (unknown)
  ORGANISM  Saccharomyces cerevisiae Saccharomyces cerevisiae Eukaryota; Fungi;
            Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales;
            Saccharomycetaceae; Saccharomyces
COMMENT     Imported using the Genbank importer. File name:
            pDIV271_gRNA1_GUS_Kp_GAPp_hsCas9_NatOPT.gbk
FEATURES             Location/Qualifiers
     misc_feature    2..536
                     /label="KU70 Upstream "
     promoter        548..967
                     /label="Sc.TEF1p"
     CDS             977..2788
                     /label="uidA_OPT"
     terminator      2803..2988
                     /label="Sc.CYC1t"
     misc_feature    2996..3618
                     /label="KU70 Downstream"
     rep_origin      4395..5060
                     /label="ori"
     gene            complement(5160..6020)
                     /label="bla"
                     /label="AmpR"
ORIGIN
        1 TTTCACCTAC GGGTCTGACT ACCTGGGCCC ATATTGGGAT CCTGGCCGTA CACATTTCAG
       61 AAATCATTTA AAGGAGTTGA ATCACATCTT ACTGGATAGC GAGCTTTTTG ACGAAGTGAA
      121 AATTTCTAAT TTTAAACAAG AGGAAGGGGT CAAAAACGGA GATATCTTAT ACTTGGAAAA
      181 AGAGATGACA ATCAGTGATT TCATCAATTT TGTATCTAGT TGGCCTTCTG TGTTTTCGTG
      241 GAAGCAGCAA CGAGGAAAGG AGGGTATCCT AGATGATTTT TACAACGAAC TGAACGACTG
      301 CTTTGAGGGG GGTAACATGA AAGTAATATG GAACTCCGTC CTAGTATTTG CCAGGAGGAA
      361 GCAAAGGGTT GTATAGGCTT TAGTACTTAT AGAGGAAACG GGGTTACGTG CAAGCGCGCA
      421 TGCCTGAGCT TTGAGGGGGG GGACTTTCAC ATCTCTTCTT CTCACACTTA GCCCTAACAC
      481 AGAGAATAAT AAAAAGCATT GCAAGATGAG TGTTGTCAGC AAGCAATACG ACATCCAAAG
      541 CATTGCGCAC ACACCATAGC TTCAAAATGT TTCTACTCCT TTTTTACTCT TCCAGATTTT
      601 CTCGGACTCC GCGCATCGCC GTACCACTTC AAAACACCCA AGCACAGCAT ACTAAATTTC
      661 CCCTCTTTCT TCCTCTAGGG TGTCGTTAAT TACCCGTACT AAAGGTTTGG AAAAGAAAAA
      721 AGAGACCGCC TCGTTTCTTT TTCTTCGTCG AAAAAGGCAA TAAAAATTTT TATCACGTTT
      781 CTTTTTCTTG AAAATTTTTT TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT
      841 TTAAGTTAAT AAACGGTCTT CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA
      901 CAACTTTTTT TACTTCTTGC TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTAA
      961 TTACAAAATC TGTCATATGT TGCGACCAGT AGAGACCCCT ACAAGAGAGA TCAAAAAACT
     1021 TGACGGTCTT TGGGCCTTTA GTCTTGACAG AGAGAATTGC GGTATTGACC AGCGATGGTG
     1081 GGAGTCCGCT TTGCAGGAGT CTAGAGCCAT TGCCGTCCCT GGTTCATTCA ATGATCAGTT
     1141 CGCCGATGCT GATATTCGTA ACTACGCAGG TAATGTTTGG TACCAGCGAG AAGTTTTTAT
     1201 CCCTAAAGGT TGGGCAGGTC AACGAATCGT TCTTCGTTTC GATGCAGTGA CCCACTATGG
     1261 AAAGGTCTGG GTGAACAACC AGGAAGTTAT GGAGCACCAA GGTGGGTATA CTCCATTCGA
     1321 AGCTGATGTA ACACCATACG TTATCGCTGG GAAATCAGTA CGAATCACAG TCTGTGTAAA
     1381 CAACGAACTT AACTGGCAGA CTATCCCACC AGGTATGGTG ATAACTGACG AGAATGGTAA
     1441 AAAAAAGCAG TCTTATTTCC ATGACTTTTT CAACTACGCA GGGATACATC GTTCAGTAAT
     1501 GTTGTATACA ACTCCTAACA CCTGGGTGGA CGACATAACC GTCGTCACCC ATGTGGCACA
     1561 GGACTGCAAT CACGCTTCTG TTGATTGGCA AGTGGTCGCA AACGGAGACG TGAGTGTCGA
     1621 GTTGCGTGAT GCTGACCAAC AGGTAGTGGC AACCGGTCAG GGTACCTCCG GGACACTTCA
     1681 AGTCGTCAAC CCACACTTAT GGCAACCAGG GGAGGGATAC CTTTATGAAT TGTGTGTAAC
     1741 CGCAAAATCA CAAACAGAAT GTGATATCTA CCCTCTTCGA GTCGGTATCA GATCCGTAGC
     1801 CGTGAAGGGG GAGCAGTTTT TGATAAACCA CAAACCTTTC TACTTCACAG GATTTGGGAG
     1861 ACATGAGGAT GCTGACCTTC GAGGTAAGGG ATTTGACAAT GTTCTTATGG TGCACGATCA
     1921 TGCTTTGATG GATTGGATTG GTGCCAACAG TTACAGAACT AGTCACTACC CATATGCCGA
     1981 AGAGATGCTT GATTGGGCAG ATGAGCATGG GATCGTAGTC ATAGACGAGA CCGCCGCCGT
     2041 AGGATTCAAT TTATCCTTAG GAATCGGTTT TGAAGCTGGG AATAAGCCTA AGGAACTTTA
     2101 CTCCGAGGAG GCTGTAAACG GAGAGACCCA ACAGGCACAC CTTCAGGCCA TTAAGGAGTT
     2161 GATCGCAAGA GATAAAAACC ACCCATCCGT TGTTATGTGG TCCATTGCCA ATGAGCCAGA
     2221 CACACGACCT CAAGGAGCCC GTGAATACTT CGCCCCACTT GCAGAAGCTA CACGTAAATT
     2281 AGATCCAACC CGACCAATTA CATGCGTAAA CGTCATGTTT TGCGACGCCC ACACTGACAC
     2341 AATTTCCGAC CTTTTTGACG TGTTGTGCCT TAACCGTTAC TATGGGTGGT ATGTGCAATC
     2401 AGGAGACTTA GAGACAGCAG AAAAAGTCTT AGAGAAAGAG CTTCTTGCTT GGCAGGAAAA
     2461 ACTTCACCAA CCAATTATTA TTACCGAGTA TGGGGTGGAC ACCCTTGCAG GATTGCATAG
     2521 TATGTATACT GACATGTGGA GTGAAGAGTA CCAGTGTGCC TGGTTAGACA TGTATCACCG
     2581 TGTGTTTGAC AGAGTTTCTG CAGTTGTAGG TGAGCAGGTG TGGAATTTCG CCGATTTCGC
     2641 CACATCACAA GGTATATTGC GTGTAGGAGG TAACAAAAAG GGTATATTCA CAAGAGATCG
     2701 AAAGCCTAAA TCTGCCGCTT TCTTACTTCA GAAGCGTTGG ACCGGGATGA ATTTTGGAGA
     2761 GAAGCCTCAG CAGGGAGGTA AACAATGAAT CGCGTGCATT CATCCGCTCT AACCGAAAAG
     2821 GAAGGAGTTA GACAACCTGA AGTCTAGGTC CCTATTTATT TTTTTATAGT TATGTTAGTA
     2881 TTAAGAACGT TATTTATATT TCAAATTTTT CTTTTTTTTC TGTACAGACG CGTGTACGCA
     2941 TGTAACATTA TACTGAAAAC CTTGCTTGAG AAGGTTTTGG GACGCTCGAA GGTGTCGGCT
     3001 TCAGAAGGGA AATCTCAGCT CCAGATCATC TTAGAGAATG TCAGTGAGGT TATTTCTGAG
     3061 CTAATCATTA CCTTGCCCGG TACAGGAATA GGGTGTTACC TTATTAATTA CGACGGTGGT
     3121 CAAAACGACG AAATTTACCC CATTTTTGAG TTACAAGACC TGAATTTGGA AATGATGAAA
     3181 CAATTGTACC AAGTCTTGGA GGACCATGTA AGTGGGCTTA ATCCTCTCGA GAAGCAATTC
     3241 CCAATTGAAC ACAGTAAACC GTTATCAGCC ACTCTGTTCT TTCACTTAAG GTCTCTTTTT
     3301 TACATGGCGA AGACTCATAA GCGTACTGGA AGACATTACA ACTTGAAAAA GATTTTCTTG
     3361 TTCACTAATA ACGATAAACC TTACAATGGA AACTCTCAGC TGAGAGTTCC CTTGAAGAAA
     3421 ACCCTGGCTG ATTACAATGA CGTAGACATT ACTTTGATTC CGTTTCTTCT GAACAAGCCT
     3481 TCAGGTGTCA AGTTTGACAA GACGGAATAC TCAGAAATTT TGTTCTATGA TAAAGATGCT
     3541 TGTTCGATGT CAATTGAGGA GATCCGCCAA CGAATTTCTA GACATAAGGA GATCAAGCGG
     3601 GTTTACTTCA CCTGTCCTAT TAAGACCTCA GCGCGGCCGC AAATTTAAAT AAAATGAAGT
     3661 GAAGTTCCTA TACTTTCTAG AGAATAGGAA CTTCTATAGT GAGTCGAATA AGGGCGACAC
     3721 AAAATTTATT CTAAATGCAT AATAAATACT GATAACATCT TATAGTTTGT ATTATATTTT
     3781 GTATTATCGT TGACATGTAT AATTTTGATA TCAAAAACTG ATTTTCCCTT TATTATTTTC
     3841 GAGATTTATT TTCTTAATTC TCTTTAACAA ACTAGAAATA TTGTATATAC AAAAAATCAT
     3901 AAATAATAGA TGAATAGTTT AATTATAGGT GTTCATCAAT CGAAAAAGCA ACGTATCTTA
     3961 TTTAAAGTGC GTTGCTTTTT TCTCATTTAT AAGGTTAAAT AATTCTCATA TATCAAGCAA
     4021 AGTGACAGGC GCCCTTAAAT ATTCTGACAA ATGCTCTTTC CCTAAACTCC CCCCATAAAA
     4081 AAACCCGCCG AAGCGGGTTT TTACGTTATT TGCGGATTAA CGATTACTCG TTATCAGAAC
     4141 CGCCCAGGGG GCCCGAGCTT AAGACTGGCC GTCGTTTTAC AACACAGAAA GAGTTTGTAG
     4201 AAACGCAAAA AGGCCATCCG TCAGGGGCCT TCTGCTTAGT TTGATGCCTG GCAGTTCCCT
     4261 ACTCTCGCCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG
     4321 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC
     4381 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT
     4441 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG
     4501 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC
     4561 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC
     4621 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT
     4681 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT
     4741 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC
     4801 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA
     4861 GTGGTGGGCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA
     4921 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG
     4981 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA
     5041 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGACGCGC GCGTAACTCA
     5101 CGTTAAGGGA TTTTGGTCAT GAGCTTGCGC CGTCCCGTCA AGTCAGCGTA ATGCTCTGCT
     5161 TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG TTCATCCATA
     5221 GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC ATCTGGCCCC
     5281 AGCGCTGCGA TGATACCGCG AGAACCACGC TCACCGGCTC CGGATTTATC AGCAATAAAC
     5341 CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC CTCCATCCAG
     5401 TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG TTTGCGCAAC
     5461 GTTGTTGCCA TCGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT GGCTTCATTC
     5521 AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG CAAAAAAGCG
     5581 GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT GTTATCACTC
     5641 ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG ATGCTTTTCT
     5701 GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG ACCGAGTTGC
     5761 TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT AAAAGTGCTC
     5821 ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT GTTGAGATCC
     5881 AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC TTTCACCAGC
     5941 GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT AAGGGCGACA
     6001 CGGAAATGTT GAATACTCAT ATTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT
     6061 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTC
     6121 AGTGTTACAA CCAATTAACC AATTCTGAAC ATTATCGCGA GCCCATTTAT ACCTGAATAT
     6181 GGCTCATAAC ACCCCTTGTT TGCCTGGCGG CAGTAGCGCG GTGGTCCCAC CTGACCCCAT
     6241 GCCGAACTCA GAAGTGAAAC GCCGTAGCGC CGATGGTAGT GTGGGGACTC CCCATGCGAG
     6301 AGTAGGGAAC TGCCAGGCAT CAAATAAAAC GAAAGGCTCA GTCGAAAGAC TGGGCCTTTC
     6361 GCCCGGGCTA ATTATGGGGT GTCGCCCTTA TTCGACTCTA TAGTGAAGTT CCTATTCTCT
     6421 AGAAAGTATA GGAACTTCTG AAGTGGGGAT TTAAATGCGG CCGCGCTGAG GGTTTAA
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