LOCUS       Linear_GFP              4444 bp    DNA        circular     26-AUG-2024
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    206..224
                     /note="locus_tag: T7 label: T7 ApEinfo_revcolor: green
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    225..272
                     /note="locus_tag: 5' UTR label: 5' UTR ApEinfo_revcolor:
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="5' UTR"
                     /label="5' UTR"
                     /ApEinfo_label="5' UTR"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    279..284
                     /note="locus_tag: Kozak label: Kozak ApEinfo_revcolor:
                     green ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_label="Kozak"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             285..1004
                     /note="product: the original enhanced GFP (Yang et al.,
                     1996) note: mammalian codon-optimized translation:
                     MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPW
                     PTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFE
                     GDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIED
                     GSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITL
                     GMDELYK locus_tag: EGFP label: EGFP ApEinfo_revcolor:
                     #e9d024 ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0
                     0 -1 0-0.5} {} 0} width 5 offset 0"
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             285..1004
                     /transl_table="11"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP(1)"
                     /label="EGFP(1)"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1011..1299
                     /note="locus_tag: AES-mtRNRI label: AES-mtRNRI
                     ApEinfo_revcolor: green ApEinfo_graphicformat: arrow_data
                     {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
                     /locus_tag="AES-mtRNRI"
                     /label="AES-mtRNRI"
                     /ApEinfo_label="AES-mtRNRI"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2397..3257
                     /note="locus_tag: Amp-R label: Amp-R ApEinfo_revcolor:
                     #b1ff67 ApEinfo_graphicformat: arrow_data {{0 0.5 0 1 2 0
                     0 -1 0-0.5} {} 0} width 5 offset 0"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagTAATA CGACTCACTA TAAGGAGAAT AAACTAGTAT
      241 TCTTCTGGTC CCCACAGACT CAGAGAGAAC CCACGCGTGC CACCatggtg agcaagggcg
      301 aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc
      361 acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga
      421 agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga
      481 cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca
      541 agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca
      601 actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac cgcatcgagc
      661 tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg gagtacaact
      721 acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc aaggtgaact
      781 tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac taccagcaga
      841 acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg agcacccagt
      901 ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg gagttcgtga
      961 ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaaGTCGAC CTGGTACTGC
     1021 ATGCACGCAA TGCTAGCTGC CCCTTTCCCG TCCTGGGTAC CCCGAGTCTC CCCCGACCTC
     1081 GGGTCCCAGG TATGCTCCCA CCTCCACCTG CCCCACTCAC CACCTCTGCT AGTTCCAGAC
     1141 ACCTCCCAAG CACGCAGCAA TGCAGCTCAA AACGCTTAGC CTAGCCACAC CCCCACGGGA
     1201 AACAGCAGTG ATTAACCTTT AGCAATAAAC GAAAGTTTAA CTAAGCTATA CTAACCCCAG
     1261 GGTTGGTCAA TTTCGTGCCA GCCACACCCT GGAGCTAGCA AAAAAAAAAA AAAAAAAAAA
     1321 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     1381 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAt ttttttctcg agttaagggc gaattcccga
     1441 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     1501 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     1561 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     1621 agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact
     1681 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     1741 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     1801 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2041 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2221 aaaaatttaa cgcgaatttt aacaaaatat taacgtttat aatttcaggt ggcatctttc
     2281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     2341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     2401 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     2461 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     2521 tgggttacat cgaactggat ctcaatagtg gtaagatcct tgagagtttt cgccccgaag
     2581 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     2641 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     2701 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     2761 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     2821 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     2881 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     2941 tagtaatggt aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3001 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     3061 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     3121 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     3181 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     3241 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     3301 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     3361 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     3421 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     3481 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     3541 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     3601 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     3661 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     3721 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     3781 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     3841 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     3901 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     3961 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4021 ccagcaacgc ggccttttta cggttcctgg ccttttgctg cggttttgct cacatgttct
     4081 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     4141 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     4201 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     4261 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     4321 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     4381 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc agatttaatt
     4441 aagg     
//
