LOCUS       Circular_ic_CVB3        5622 bp    DNA        circular     26-AUG-2024
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    215..233
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    234..289
                     /locus_tag="P3 twister ribozyme"
                     /label="P3 twister ribozyme"
                     /ApEinfo_label="P3 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    290..310
                     /locus_tag="Ligation stem"
                     /label="Ligation stem"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    342..1082
                     /note="locus_tag: CVB3 label: CVB3 ApEinfo_revcolor: green
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="CVB3"
                     /label="CVB3"
                     /ApEinfo_label="CVB3"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1083..1802
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1083..1802
                     /transl_table="11"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP(1)"
                     /label="EGFP(1)"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1809..2337
                     /locus_tag="Modified WPRE"
                     /label="Modified WPRE"
                     /ApEinfo_label="Modified WPRE"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2342..2506
                     /locus_tag="165 PolyA"
                     /label="165 PolyA"
                     /ApEinfo_label="165 PolyA"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2507..2532
                     /locus_tag="Ligation stem(1)"
                     /label="Ligation stem(1)"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2533..2587
                     /locus_tag="P1 twister ribozyme"
                     /label="P1 twister ribozyme"
                     /ApEinfo_label="P1 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3575..4435
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcgccc ttaagctagc atgcTAATAC GACTCACTAT AGGGCCATCA
      241 GTCGCCGGTC CCAAGCCCGG ATAAAATGGG AGGGGGCGGG AAACCGCCTA ACCATGCCGA
      301 CTGATGGCAG agcggccgcg tcgacgggcc cgcggaattc cTTAAAACAG CCTGTGGGTT
      361 GATCCCACCC ACAGGCCCAT TGGGCGCTAG CACTCTGGTA TCACGGTACC TTTGTGCGCC
      421 TGTTTTATAC CCCCTCCCCC AACTGTAACT TAGAAGTAAC ACACACCGAT CAACAGTCAG
      481 CGTGGCACAC CAGCCACGTT TTGATCAAGC ACTTCTGTTA CCCCGGACTG AGTATCAATA
      541 GACTGCTCAC GCGGTTGAAG GAGAAAGCGT TCGTTATCCG GCCAACTACT TCGAAAAACC
      601 TAGTAACACC GTGGAAGTTG CAGAGTGTTT CGCTCAGCAC TACCCCAGTG TAGATCAGGT
      661 CGATGAGTCA CCGCATTCCC CACGGGCGAC CGTGGCGGTG GCTGCGTTGG CGGCCTGCCC
      721 ATGGGGAAAC CCATGGGACG CTCTAATACA GACATGGTGC GAAGAGTCTA TTGAGCTAGT
      781 TGGTAGTCCT CCGGCCCCTG AATGCGGCTA ATCCTAACTG CGGAGCACAC ACCCTCAAGC
      841 CAGAGGGCAG TGTGTCGTAA CGGGCAACTC TGCAGCGGAA CCGACTACTT TGGGTGTCCG
      901 TGTTTCATTT TATTCCTATA CTGGCTGCTT ATGGTGACAA TTGAGAGATC GTTACCATAT
      961 AGCTATTGGA TTGGCCATCC GGTGACTAAT AGAGCTATTA TATATCCCTT TGTTGGGTTT
     1021 ATACCACTTA GCTTGAAAGA GGTTAAAACA TTACAATTCA TTGTTAAGTT GAATACAGCA
     1081 AAatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     1141 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     1201 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     1261 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     1321 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     1381 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     1441 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     1501 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     1561 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     1621 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     1681 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     1741 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt
     1801 aaggatccaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     1861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     1921 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     1981 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     2041 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     2101 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     2161 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     2221 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacaatccag
     2281 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgaagta
     2341 aAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     2401 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     2461 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAACTGC CATCAGTCGG
     2521 CGTGGACTGT AGAACACTGC CAATGCCGGT CCCAAGCCCG GATAAAAGTG GAGGGTACAG
     2581 TCCACGCttt ttttctcgag ttaagggcga attcccgata aggatcttcc tagagcatgg
     2641 ctacgtagat aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga
     2701 gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc
     2761 ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta
     2821 acctaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     2881 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     2941 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     3001 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     3061 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     3121 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     3181 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     3241 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     3301 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     3361 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     3421 caaaatatta acgtttataa tttcaggtgg catctttcgg ggaaatgtgc gcggaacccc
     3481 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     3541 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     3601 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     3661 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     3721 caatagtggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     3781 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     3841 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     3901 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     3961 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     4021 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     4081 agccatacca aacgacgagc gtgacaccac gatgcctgta gtaatggtaa caacgttgcg
     4141 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     4201 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     4261 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     4321 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     4381 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     4441 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     4501 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     4561 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     4621 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     4681 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     4741 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     4801 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     4861 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     4921 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     4981 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     5041 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     5101 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     5161 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     5221 gttcctggcc ttttgctgcg gttttgctca catgttcttt cctgcgttat cccctgattc
     5281 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     5341 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     5401 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     5461 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     5521 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     5581 aggaaacagc tatgaccatg attacgccag atttaattaa gg 
//
