LOCUS       Circular_oc_EMCV        6193 bp    DNA        circular     26-AUG-2024
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2186..2204
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2205..2260
                     /locus_tag="P3 twister ribozyme"
                     /label="P3 twister ribozyme"
                     /ApEinfo_label="P3 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2261..2281
                     /locus_tag="Ligation stem"
                     /label="Ligation stem"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2282..2381
                     /locus_tag="Exon-Intron 1"
                     /label="Exon-Intron 1"
                     /ApEinfo_label="Exon-Intron 1"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2412..2997
                     /note="internal ribosome entry site (IRES) of
                     theencephalomyocarditis virus (EMCV)"
                     /locus_tag="EMCV 6A"
                     /label="EMCV 6A"
                     /ApEinfo_label="EMCV 6A"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2998..3717
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2998..3717
                     /transl_table="11"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP(1)"
                     /label="EGFP(1)"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3724..4252
                     /locus_tag="Modified WPRE"
                     /label="Modified WPRE"
                     /ApEinfo_label="Modified WPRE"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4253..4417
                     /locus_tag="165 PolyA"
                     /label="165 PolyA"
                     /ApEinfo_label="165 PolyA"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4418..5048
                     /locus_tag="Exon-Intron 2"
                     /label="Exon-Intron 2"
                     /ApEinfo_label="Exon-Intron 2"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5049..5074
                     /locus_tag="Ligation stem(1)"
                     /label="Ligation stem(1)"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5075..5129
                     /locus_tag="P1 twister ribozyme"
                     /label="P1 twister ribozyme"
                     /ApEinfo_label="P1 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(6117..6193,1..784)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
       61 actggatctc aatagtggta agatccttga gagttttcgc cccgaagaac gttttccaat
      121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag taatggtaac
      481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1561 ctttttacgg ttcctggcct tttgctgcgg ttttgctcac atgttctttc ctgcgttatc
     1621 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1741 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1921 atttcacaca ggaaacagct atgaccatga ttacgccaga tttaattaag gctgcgcgct
     1981 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
     2041 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     2101 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
     2161 ggaattcgcc cttaagctag catgcTAATA CGACTCACTA TAGGGCCATC AGTCGCCGGT
     2221 CCCAAGCCCG GATAAAATGG GAGGGGGCGG GAAACCGCCT AACCATGCCG ACTGATGGCA
     2281 Gctattatcg agcgaacgcc ttatgcgatg aaagtcgcac gtagggtgta gaccaagcga
     2341 aatcctatgc atttaggata gtgaggtata gcaaaggaga agtcgacggg cccgcggaat
     2401 tccgcccccc ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa
     2461 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
     2521 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
     2581 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
     2641 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
     2701 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
     2761 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
     2821 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
     2881 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaacgt ctaggccccc
     2941 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     3001 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     3061 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     3121 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     3181 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     3241 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     3301 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     3361 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     3421 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     3481 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     3541 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     3601 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     3661 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagga
     3721 tccaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3781 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3841 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3901 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3961 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4021 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4081 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     4141 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacaa tccagcggac
     4201 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg aaAAAAAAAA
     4261 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     4321 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     4381 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAact atagccatac aataaaagtg
     4441 cgaaacgtta tcctataagt aagaaagttt taaaattttc ttacgaaaag gatagaactt
     4501 aaaagttcta actgttctac taaagtaata agtgaaaatc ttatttaaag caaacaacca
     4561 agtagcttta agtctaagtc ccctacacaa gttttatact actatgcaaa acttgtgaag
     4621 ctaggtaagg tcgtaatccg tgaaagtcgg atgcggggct ccttaaaaga ttactatggt
     4681 aaacataagc taatccatta agatgcgatt tatatgtatt ttatactgtt aaatattttt
     4741 gtgcttgtgg cttggtataa aacagttaag atgaagtact taactggttt tggaataatt
     4801 ggttgttaaa ctaaaacatt ataaatcgtt agtggatacc taaggtaatc aaaaataggg
     4861 ataggtagaa tggaacgttt gatgctgtat atgaagaggt ttagtagaac ctaggacaca
     4921 tatacgggct cagcaggttc atagtagcta tgatactcag ccggaagtca aattaatttt
     4981 gaaatacttc tatggtaaca taggagaagg ataaaactga gtgagccaag gaacctagtc
     5041 ggtaatagCT GCCATCAGTC GGCGTGGACT GTAGAACACT GCCAATGCCG GTCCCAAGCC
     5101 CGGATAAAAG TGGAGGGTAC AGTCCACGCt ttttttctcg agttaagggc gaattcccga
     5161 taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact
     5221 acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg
     5281 aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg
     5341 agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact
     5401 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     5461 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     5521 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     5581 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     5641 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     5701 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     5761 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     5821 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     5881 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     5941 aaaaatttaa cgcgaatttt aacaaaatat taacgtttat aatttcaggt ggcatctttc
     6001 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     6061 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     6121 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     6181 ttgctcaccc aga    
//
