LOCUS       Circular_oc_CVB3        6340 bp    DNA        circular     26-AUG-2024
DEFINITION  .
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    2186..2204
                     /locus_tag="T7"
                     /label="T7"
                     /ApEinfo_label="T7"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2205..2260
                     /locus_tag="P3 twister ribozyme"
                     /label="P3 twister ribozyme"
                     /ApEinfo_label="P3 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2261..2281
                     /locus_tag="Ligation stem"
                     /label="Ligation stem"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2282..2381
                     /locus_tag="Exon-Intron 1"
                     /label="Exon-Intron 1"
                     /ApEinfo_label="Exon-Intron 1"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2404..3144
                     /note="locus_tag: CVB3 label: CVB3 ApEinfo_revcolor: green
                     ApEinfo_graphicformat: arrow_data {{0 1 2 0 0 -1} {}
                     0}width 5 offset 0"
                     /locus_tag="CVB3"
                     /label="CVB3"
                     /ApEinfo_label="CVB3"
                     /ApEinfo_fwdcolor="#008080"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3145..3864
                     /locus_tag="EGFP"
                     /label="EGFP"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3145..3864
                     /transl_table="11"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
                     LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
                     DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK*"
                     /locus_tag="EGFP(1)"
                     /label="EGFP(1)"
                     /ApEinfo_label="EGFP"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3871..4399
                     /locus_tag="Modified WPRE"
                     /label="Modified WPRE"
                     /ApEinfo_label="Modified WPRE"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4400..4564
                     /locus_tag="165 PolyA"
                     /label="165 PolyA"
                     /ApEinfo_label="165 PolyA"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4565..5195
                     /locus_tag="Exon-Intron 2"
                     /label="Exon-Intron 2"
                     /ApEinfo_label="Exon-Intron 2"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5196..5221
                     /locus_tag="Ligation stem(1)"
                     /label="Ligation stem(1)"
                     /ApEinfo_label="Ligation stem"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5222..5276
                     /locus_tag="P1 twister ribozyme"
                     /label="P1 twister ribozyme"
                     /ApEinfo_label="P1 twister ribozyme"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             join(6264..6340,1..784)
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVVMVTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
                     /locus_tag="Amp-R"
                     /label="Amp-R"
                     /ApEinfo_label="Amp-R"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
       61 actggatctc aatagtggta agatccttga gagttttcgc cccgaagaac gttttccaat
      121 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
      181 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
      241 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
      301 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
      361 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
      421 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag taatggtaac
      481 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
      541 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
      601 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
      661 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
      721 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
      781 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
      841 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
      901 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
      961 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     1021 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     1081 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     1141 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     1201 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     1261 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     1321 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     1381 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     1441 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     1501 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     1561 ctttttacgg ttcctggcct tttgctgcgg ttttgctcac atgttctttc ctgcgttatc
     1621 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     1681 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     1741 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga
     1801 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     1861 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     1921 atttcacaca ggaaacagct atgaccatga ttacgccaga tttaattaag gctgcgcgct
     1981 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
     2041 gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac taggggttcc
     2101 ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc taggaagatc
     2161 ggaattcgcc cttaagctag catgcTAATA CGACTCACTA TAGGGCCATC AGTCGCCGGT
     2221 CCCAAGCCCG GATAAAATGG GAGGGGGCGG GAAACCGCCT AACCATGCCG ACTGATGGCA
     2281 Gctattatcg agcgaacgcc ttatgcgatg aaagtcgcac gtagggtgta gaccaagcga
     2341 aatcctatgc atttaggata gtgaggtata gcaaaggaga agtcgacggg cccgcggaat
     2401 tccTTAAAAC AGCCTGTGGG TTGATCCCAC CCACAGGCCC ATTGGGCGCT AGCACTCTGG
     2461 TATCACGGTA CCTTTGTGCG CCTGTTTTAT ACCCCCTCCC CCAACTGTAA CTTAGAAGTA
     2521 ACACACACCG ATCAACAGTC AGCGTGGCAC ACCAGCCACG TTTTGATCAA GCACTTCTGT
     2581 TACCCCGGAC TGAGTATCAA TAGACTGCTC ACGCGGTTGA AGGAGAAAGC GTTCGTTATC
     2641 CGGCCAACTA CTTCGAAAAA CCTAGTAACA CCGTGGAAGT TGCAGAGTGT TTCGCTCAGC
     2701 ACTACCCCAG TGTAGATCAG GTCGATGAGT CACCGCATTC CCCACGGGCG ACCGTGGCGG
     2761 TGGCTGCGTT GGCGGCCTGC CCATGGGGAA ACCCATGGGA CGCTCTAATA CAGACATGGT
     2821 GCGAAGAGTC TATTGAGCTA GTTGGTAGTC CTCCGGCCCC TGAATGCGGC TAATCCTAAC
     2881 TGCGGAGCAC ACACCCTCAA GCCAGAGGGC AGTGTGTCGT AACGGGCAAC TCTGCAGCGG
     2941 AACCGACTAC TTTGGGTGTC CGTGTTTCAT TTTATTCCTA TACTGGCTGC TTATGGTGAC
     3001 AATTGAGAGA TCGTTACCAT ATAGCTATTG GATTGGCCAT CCGGTGACTA ATAGAGCTAT
     3061 TATATATCCC TTTGTTGGGT TTATACCACT TAGCTTGAAA GAGGTTAAAA CATTACAATT
     3121 CATTGTTAAG TTGAATACAG CAAAatggtg agcaagggcg aggagctgtt caccggggtg
     3181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
     3241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     3301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     3361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     3421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     3481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     3541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     3601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     3661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     3721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     3781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     3841 ggcatggacg agctgtacaa gtaaggatcc aatcaacctc tggattacaa aatttgtgaa
     3901 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3961 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4021 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4081 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4141 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4201 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4261 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4321 acgtccttct gctacaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4381 cctcttccgc gtcttcgaaA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     4441 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     4501 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
     4561 AAAAactata gccatacaat aaaagtgcga aacgttatcc tataagtaag aaagttttaa
     4621 aattttctta cgaaaaggat agaacttaaa agttctaact gttctactaa agtaataagt
     4681 gaaaatctta tttaaagcaa acaaccaagt agctttaagt ctaagtcccc tacacaagtt
     4741 ttatactact atgcaaaact tgtgaagcta ggtaaggtcg taatccgtga aagtcggatg
     4801 cggggctcct taaaagatta ctatggtaaa cataagctaa tccattaaga tgcgatttat
     4861 atgtatttta tactgttaaa tatttttgtg cttgtggctt ggtataaaac agttaagatg
     4921 aagtacttaa ctggttttgg aataattggt tgttaaacta aaacattata aatcgttagt
     4981 ggatacctaa ggtaatcaaa aatagggata ggtagaatgg aacgtttgat gctgtatatg
     5041 aagaggttta gtagaaccta ggacacatat acgggctcag caggttcata gtagctatga
     5101 tactcagccg gaagtcaaat taattttgaa atacttctat ggtaacatag gagaaggata
     5161 aaactgagtg agccaaggaa cctagtcggt aatagCTGCC ATCAGTCGGC GTGGACTGTA
     5221 GAACACTGCC AATGCCGGTC CCAAGCCCGG ATAAAAGTGG AGGGTACAGT CCACGCtttt
     5281 tttctcgagt taagggcgaa ttcccgataa ggatcttcct agagcatggc tacgtagata
     5341 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     5401 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     5461 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac
     5521 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     5581 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     5641 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     5701 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     5761 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     5821 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     5881 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     5941 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6001 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6061 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6121 cgtttataat ttcaggtggc atctttcggg gaaatgtgcg cggaacccct atttgtttat
     6181 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     6241 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     6301 tttttgcggc attttgcctt cctgtttttg ctcacccaga  
//
