LOCUS       pJB65_(VTGless_MR1-5_fo 5700 bp ds-DNA     circular     02-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    complement(404..629)
                     /label="ROP/ROM"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer_bind     2157..2176
                     /label="T7"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2236..2238
                     /label="CDS"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     CDS             2236..3327
                     /label="Translation 2236-3327"
                     /translation="MSHHHHHHAPKKVETGHTLSGGSAAATQVDRKSHAAANEPGTCRAAAATEYVGTEQFTSFCNTSPKPNATKVNVTTTARGRPAAATEVSRTEKAAANESGVCRNAAATEYMSNEAHFSLCGTAAKPSQADKVMFGATARTHQVAAASDEFRPSSAAANESGAKRTITGSQYADEGLARLTINGAPAKVARTHTFAGSDAAATEIGRSTRAAADESGSCRSISGTEYLSNEQFQSFCDTKPQRSPFKVGQDRTNKGQSAAANLVDRSELAAANEPGSCSRAAASQYGQSKICGGGVGKVRSMRTLRGTSVSGQQLDHAPKMSGDERGGCMPVTGNEYYGREHFEPFCTSTPEPEAQWSHPQFEK*"
     misc_feature    2238..2241
                     /label="Recombination Site A"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2242..2259
                     /label="His-tag"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(2247..2268)
                     /label="pBz84 (6xHis-MR1-6-Strep) REV"
                     /note="sequence: GCagatccgccggatagggtgtgaccggtttcaaccttttttggcgcATGATGATGATGA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            2260..2268
                     /label="Hneap_0920"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             2260..2268
                     /label="Hneap_0920 (CsoS2)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    2260..2268
                     /label="RADAR repeat 3"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2269..2445
                     /label="MR1_WT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2305..2313
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2338..2346
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2371..2379
                     /label="VSG->AAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2446..2625
                     /label="MR2_WT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2482..2490
                     /label="VSG->AAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2515..2523
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2548..2556
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2626..2793
                     /label="MR3_WT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2665..2673
                     /label="VSG->AAA"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2697..2705
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2794..2970
                     /label="MR4_WT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    2830..2838
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2863..2871
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2971..3123
                     /label="MR5_WT"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    3006..3014
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3040..3048
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3073..3081
                     /label="VTG -> AAA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          3124..3140
                     /label="pBz84 (6xHis-MR1-6-Strep) FWD"
                     /note="sequence: ctctcagtatggccaaagcaaaatctgcggtggtggcgtgggaaaagtgcgctcaatgcg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            3124..3185
                     /label="Hneap_0920"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             3124..3185
                     /label="Hneap_0920 (CsoS2)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     misc_feature    3124..3273
                     /label="Cheryl's M-repeat 6"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     CDS             3187..3300
                     /label="Hneap_0920 (CsoS2)"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
     gene            3187..3300
                     /label="Hneap_0920"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     gene            3187..3300
                     /label="carboxysome operon"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(3282..3300)
                     /label="JB169"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          3283..3300
                     /label="JB177"
                     /note="sequence: ggtcgtgaacatttcgaaccgttttgtacgagcaccccagagcccgaagctcaa"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3301..3324
                     /label="Strep-Tag II"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    3301..3327
                     /label="JB168"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    3328..3331
                     /label="Recombination Site B"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3407..3447
                     /label="T7 Terminator"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             4059..4916
                     /label="AmpR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     rep_origin      5014..5696
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#808080"
                     /ApEinfo_fwdcolor="#808080"
     misc_feature    complement(5126..5235)
                     /label="RNA1-for"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(5674..5683)
                     /label="ColE1 ORI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
ORIGIN
        1 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
       61 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
      121 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
      181 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
      241 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
      301 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
      361 tccgcttaca gacaagctgt gaccgcctcc gggagctgca tgtgtcagag gttttcaccg
      421 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
      481 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
      541 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
      601 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
      661 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
      721 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
      781 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
      841 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
      901 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
      961 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     1021 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
     1081 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
     1141 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
     1201 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
     1261 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
     1321 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga
     1381 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
     1441 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
     1501 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
     1561 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
     1621 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
     1681 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
     1741 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
     1801 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
     1861 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
     1921 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
     1981 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
     2041 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
     2101 tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat
     2161 acgactcact atagggacac cacaacggtt tccctctaga aataattttg tttaacttta
     2221 agaaggagat ataccatgtc cCACCATCAT CATCATCATg cgccaaaaaa ggttgaaacc
     2281 ggtcacaccc tatccggcgg atctGCAGCT GCAacgcaag tggatcgtaa atctcatGCT
     2341 GCAGCTaacg agccgggcac ttgccgagca GCAGCTGCTa ccgagtacgt aggtactgag
     2401 caattcacct ctttttgcaa taccagcccc aagccaaatg cgacgaaggt caatgtgacc
     2461 acaacggctc gtggtcgccc tGCTGCAGCT acggaagttt cacggaccga gaagGCAGCA
     2521 GCTaacgaat ccggtgtctg ccgtaacGCT GCTGCAaccg aatacatgag taatgaagct
     2581 cacttttctc tatgtggaac agccgcaaag ccttcacaag cggataaagt catgttcggc
     2641 gccacagcac gaacgcatca agtgGCAGCT GCTagtgatg aattcaggcc ctcttctGCT
     2701 GCAGCAaacg aatcgggtgc aaaacgcaca attaccggct cgcagtacgc agacgaaggt
     2761 cttgcgcgac tcacgatcaa cggagcacct gcaaaagtag ccagaaccca cacctttgcg
     2821 ggctctgacG CAGCTGCAac ggaaatcggt cgctctactc gcGCAGCAGC Tgatgaaagc
     2881 ggttcgtgtc gttcaatctc aggcaccgag tatctcagta acgagcaatt ccaatccttt
     2941 tgtgacacaa aacctcaacg cagcccgttc aaggttggcc aagatcgcac gaacaagggt
     3001 cagtctGCAG CTGCAaactt ggttgatcgt tccgaactgG CTGCAGCTaa cgaaccaggt
     3061 tcatgctctc ggGCTGCTGC Atctcagtat ggccaaagca aaatctgcgg tggtggcgtg
     3121 ggaaaagtgc gctcaatgcg cacccttcgc ggcacctcag tatctggcca acagctagat
     3181 catgcAccTa agatgtccgg tgacgagcgc ggcgggtgca tgcccgtcac cggtaatgag
     3241 tactacggtc gtgaacattt cgaaccgttt tgtacgagca ccccagagcc cgaagctcaa
     3301 TGGAGTCATC CACAGTTCGA AAAGtaaagc ggttaagatc cggctgctaa caaagcccga
     3361 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
     3421 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag
     3481 gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag
     3541 gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg
     3601 catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac
     3661 gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag
     3721 ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact
     3781 gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca
     3841 aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     3901 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     3961 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4021 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4081 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4141 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4201 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4261 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
     4321 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4381 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4441 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     4501 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     4561 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt
     4621 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     4681 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     4741 ttattgctga taaatctgga gccggtgagc gtggctctcg cggtatcatt gcagcactgg
     4801 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     4861 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     4921 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     4981 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5041 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5101 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5161 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5221 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5281 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5341 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5401 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     5461 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     5521 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     5581 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     5641 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
//