LOCUS       Exported                8273 bp ds-DNA     circular SYN 29-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8273)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 29, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     file made on 11-22-2019.  
            2376-78, ACA-GAC
            plamisd is confirmed by complete plasmid sequencing.  (2/12/2020)
            maxi is confirmed by Rapid Plasmid sequencing#244689N except
            deletion 3781-85 ( region between WPRE
FEATURES             Location/Qualifiers
     source          1..8273
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    232..819
                     /label=CMV
     CDS             987..3161
                     /codon_start=1
                     /label=ORF
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYKGTGEGRGSLLTCGDVEENPGPTGKGKPIPNPLLGLDS
                     TTSAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSE
                     EPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLED
                     EAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKT
                     PPAPKDPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPK
                     SPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGS
                     KDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQS
                     KIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNV
                     SSTGSIDMVDSPQLATLADEVSASLAKQGL"
     misc_feature    987..1718
                     /label=TagRFP.T
     misc_feature    1728..1781
                     /label=2A
     misc_feature    1791..1832
                     /label=V5
     misc_feature    1839..3161
                     /label=T181DTau
     misc_feature    3178..3766
                     /label=WPRE
     polyA_signal    3870..4094
                     /label=bGH
                     /note="bGH poly(A) signalbovine growth hormone 
                     polyadenylation signal"
     misc_feature    3870..4094
                     /label=bGHpA
     misc_feature    4978..5772
                     /label=Neo
     polyA_signal    5946..6067
                     /label=SV40
                     /note="SV40 poly(A) signalSV40 polyadenylation signal"
     misc_feature    complement(7278..8273)
                     /label=AMPr
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
      961 gggcccggga tccaccggtc gccaccatgg tgtctaaggg cgaagagctg attaaggaga
     1021 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     1081 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     1141 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     1201 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     1261 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     1321 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     1381 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc gagatgctgt
     1441 accccgctga cggcggcctg gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg
     1501 gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca
     1561 agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag
     1621 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     1681 tggggcacaa acttaatggc atggacgagc tgtacaaggg taccggcgag ggcagaggaa
     1741 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aaccggtaaa ggtaagccta
     1801 tccctaaccc tctcctcggt ctcgattcta cgactagtgc tgagccccgc caggagttcg
     1861 aagtgatgga agatcacgct gggacgtacg ggttggggga caggaaagat caggggggct
     1921 acaccatgca ccaagaccaa gagggtgaca cggacgctgg cctgaaagaa tctcccctgc
     1981 agacccccac tgaggacgga tctgaggaac cgggctctga aacctctgat gctaagagca
     2041 ctccaacagc ggaagatgtg acagcaccct tagtggatga gggagctccc ggcaagcagg
     2101 ctgccgcgca gccccacacg gagatcccag aaggaaccac agctgaagaa gcaggcattg
     2161 gagacacccc cagcctggaa gacgaagctg ctggtcacgt gacccaagct cgcatggtca
     2221 gtaaaagcaa agacgggact ggaagcgatg acaaaaaagc caagggggct gatggtaaaa
     2281 cgaagatcgc cacaccgcgg ggagcagccc ctccaggcca gaagggccag gccaacgcca
     2341 ccaggattcc agcaaaaacc ccgcccgctc caaaggaccc acccagctct ggtgaacctc
     2401 caaaatcagg ggatcgcagc ggctacagca gccccggctc cccaggcact cccggcagcc
     2461 gctcccgcac cccgtccctt ccaaccccac ccacccggga gcccaagaag gtggcagtgg
     2521 tccgtactcc acccaagtcg ccgtcttccg ccaagagccg cctgcagaca gcccccgtgc
     2581 ccatgccaga cctgaagaat gtcaagtcca agatcggctc cactgagaac ctgaagcacc
     2641 agccgggagg cgggaaggtg cagataatta ataagaagct ggatcttagc aacgtccagt
     2701 ccaagtgtgg ctcaaaggat aatatcaaac acgtcccggg aggcggcagt gtgcaaatag
     2761 tctacaaacc agttgacctg agcaaggtga cctccaagtg tggctcatta ggcaacatcc
     2821 atcataaacc aggaggtggc caggtggaag taaaatctga gaagcttgac ttcaaggaca
     2881 gagtccagtc gaagattggg tccctggaca atatcaccca cgtccctggc ggaggaaata
     2941 aaaagattga aacccacaag ctgaccttcc gcgagaacgc caaagccaag acagaccacg
     3001 gggcggagat cgtgtacaag tcgccagtgg tgtctgggga cacgtctcca cggcatctca
     3061 gcaatgtctc ctccaccggc agcatcgaca tggtagactc gccccagctc gccacgctag
     3121 ctgacgaggt gtctgcctcc ctggccaagc agggtttgta gggatccgat tatcgataat
     3181 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3241 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3301 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3361 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3421 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3481 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3541 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3601 tgtgttgcca cctggattct gcgcgggacg cccttctgct acgtcccttc ggccctcaat
     3661 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3721 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3781 cctagcgata tctgcagaat tccaccacac tggactagtg gatccgagct cggtaccaag
     3841 cttaagttta aaccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt
     3901 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
     3961 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
     4021 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
     4081 ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca
     4141 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     4201 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     4261 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     4321 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     4381 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     4441 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     4501 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     4561 cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     4621 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     4681 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     4741 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     4801 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag
     4861 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcccg
     4921 ggagcttgta tatccatttt cggatctgat caagagacag gatgaggatc gtttcgcatg
     4981 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     5041 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     5101 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
     5161 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
     5221 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
     5281 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
     5341 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
     5401 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
     5461 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
     5521 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
     5581 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
     5641 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
     5701 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
     5761 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
     5821 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     5881 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     5941 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     6001 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     6061 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     6121 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     6181 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     6241 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     6301 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6361 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6421 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6481 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6541 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6601 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6661 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6721 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6781 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6841 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     6901 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     6961 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7021 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     7081 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     7141 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     7201 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     7261 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     7321 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     7381 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     7441 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     7501 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     7561 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     7621 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     7681 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     7741 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     7801 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     7861 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     7921 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     7981 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     8041 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     8101 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     8161 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     8221 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
