LOCUS       Exported                8274 bp ds-DNA     circular SYN 29-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Aug 29, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     file made on 11-22-2019.  no plasmid
            2526-27:  AC-GA
            maxi is confirmed by Rapid Plasmid sequencing#244689N except
            deletion 3782-86 ( region between WPRE
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    232..819
                     /label=CMV
     CDS             987..3161
                     /codon_start=1
                     /label=ORF
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYKGTGEGRGSLLTCGDVEENPGPTGKGKPIPNPLLGLDS
                     TTSAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQTPTEDGSE
                     EPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTTAEEAGIGDTPSLED
                     EAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKT
                     PPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRDPPK
                     SPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIINKKLDLSNVQSKCGS
                     KDNIKHVPGGGSVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQS
                     KIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNV
                     SSTGSIDMVDSPQLATLADEVSASLAKQGL"
     misc_feature    987..1718
                     /label=TagRFP.T
     misc_feature    1728..1781
                     /label=2A
     misc_feature    1791..1832
                     /label=V5
     misc_feature    1839..3161
                     /label=T231DTau
     misc_feature    3179..3767
                     /label=WPRE
     polyA_signal    3871..4095
                     /label=bGH
                     /note="bGH poly(A) signalbovine growth hormone 
                     polyadenylation signal"
     misc_feature    3871..4095
                     /label=bGHpA
     misc_feature    4979..5773
                     /label=Neo
     polyA_signal    5947..6068
                     /label=SV40
                     /note="SV40 poly(A) signalSV40 polyadenylation signal"
     misc_feature    complement(7279..8274)
                     /label=AMPr
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gtttaaacgg gccctctaga ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
      961 gggcccggga tccaccggtc gccaccatgg tgtctaaggg cgaagagctg attaaggaga
     1021 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     1081 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gtggtcgagg
     1141 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcagaa
     1201 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cctgagggct
     1261 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     1321 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     1381 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggccaacacc gagatgctgt
     1441 accccgctga cggcggcctg gaaggcagaa ccgacatggc cctgaagctc gtgggcgggg
     1501 gccacctgat ctgcaacttc aagaccacat acagatccaa gaaacccgct aagaacctca
     1561 agatgcccgg cgtctactat gtggaccaca gactggaaag aatcaaggag gccgacaaag
     1621 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     1681 tggggcacaa acttaatggc atggacgagc tgtacaaggg taccggcgag ggcagaggaa
     1741 gtctgctaac atgcggtgac gtcgaggaga atcctggccc aaccggtaaa ggtaagccta
     1801 tccctaaccc tctcctcggt ctcgattcta cgactagtgc tgagccccgc caggagttcg
     1861 aagtgatgga agatcacgct gggacgtacg ggttggggga caggaaagat caggggggct
     1921 acaccatgca ccaagaccaa gagggtgaca cggacgctgg cctgaaagaa tctcccctgc
     1981 agacccccac tgaggacgga tctgaggaac cgggctctga aacctctgat gctaagagca
     2041 ctccaacagc ggaagatgtg acagcaccct tagtggatga gggagctccc ggcaagcagg
     2101 ctgccgcgca gccccacacg gagatcccag aaggaaccac agctgaagaa gcaggcattg
     2161 gagacacccc cagcctggaa gacgaagctg ctggtcacgt gacccaagct cgcatggtca
     2221 gtaaaagcaa agacgggact ggaagcgatg acaaaaaagc caagggggct gatggtaaaa
     2281 cgaagatcgc cacaccgcgg ggagcagccc ctccaggcca gaagggccag gccaacgcca
     2341 ccaggattcc agcaaaaacc ccgcccgctc caaagacacc acccagctct ggtgaacctc
     2401 caaaatcagg ggatcgcagc ggctacagca gccccggctc cccaggcact cccggcagcc
     2461 gctcccgcac cccgtccctt ccaaccccac ccacccggga gcccaagaag gtggcagtgg
     2521 tccgtgatcc acccaagtcg ccgtcttccg ccaagagccg cctgcagaca gcccccgtgc
     2581 ccatgccaga cctgaagaat gtcaagtcca agatcggctc cactgagaac ctgaagcacc
     2641 agccgggagg cgggaaggtg cagataatta ataagaagct ggatcttagc aacgtccagt
     2701 ccaagtgtgg ctcaaaggat aatatcaaac acgtcccggg aggcggcagt gtgcaaatag
     2761 tctacaaacc agttgacctg agcaaggtga cctccaagtg tggctcatta ggcaacatcc
     2821 atcataaacc aggaggtggc caggtggaag taaaatctga gaagcttgac ttcaaggaca
     2881 gagtccagtc gaagattggg tccctggaca atatcaccca cgtccctggc ggaggaaata
     2941 aaaagattga aacccacaag ctgaccttcc gcgagaacgc caaagccaag acagaccacg
     3001 gggcggagat cgtgtacaag tcgccagtgg tgtctgggga cacgtctcca cggcatctca
     3061 gcaatgtctc ctccaccggc agcatcgaca tggtagactc gccccagctc gccacgctag
     3121 ctgacgaggt gtctgcctcc ctggccaagc agggtttgta gaggatccga ttatcgataa
     3181 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     3241 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     3301 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     3361 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     3421 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     3481 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     3541 gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc
     3601 ctgtgttgcc acctggattc tgcgcgggac gcccttctgc tacgtccctt cggccctcaa
     3661 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     3721 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc gataccgtcg
     3781 acctagcgat atctgcagaa ttccaccaca ctggactagt ggatccgagc tcggtaccaa
     3841 gcttaagttt aaaccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     3901 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     3961 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     4021 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     4081 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     4141 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     4201 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     4261 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     4321 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     4381 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     4441 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     4501 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     4561 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4621 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4681 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4741 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4801 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     4861 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     4921 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat
     4981 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg
     5041 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc
     5101 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca
     5161 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct
     5221 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga
     5281 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg
     5341 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat
     5401 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga
     5461 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg
     5521 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg
     5581 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat
     5641 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct
     5701 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga
     5761 cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg
     5821 ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
     5881 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
     5941 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
     6001 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
     6061 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
     6121 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
     6181 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
     6241 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     6301 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     6361 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     6421 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     6481 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     6541 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     6601 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     6661 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     6721 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6781 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6841 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6901 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6961 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     7021 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     7081 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     7141 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     7201 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     7261 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     7321 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     7381 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     7441 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     7501 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     7561 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     7621 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     7681 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     7741 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7801 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7861 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7921 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7981 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     8041 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     8101 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     8161 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     8221 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
