LOCUS       Covid19_S_protein_Ap2u2 9002 bp ds-DNA     circular     06-MAY-2020
DEFINITION  .
COMMENT     GenBank number MN908947.3 Codon optimized for human furin site
            (RRAR) changed to A at postion 682 (2043-2046bp) K986P V987P ref1
            Immunogenicity and Protection Efficacy of Monomeric and Trimeric
            Recombinant SARS Coronavirus Spike Protein Subunit Vaccine
            Candidates
FEATURES             Location/Qualifiers
     enhancer        17..396
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        397..600
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     551..571
                     /label="CMV-F"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    644..2593
                     /label="Gene Fragment-1 S "
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        645..663
                     /label="T7 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     primer_bind     645..664
                     /label="T7"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    700..708
                     /label="Kozak"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             700..708
                     /label="Translation 700-708"
                     /translation="AAT"
     misc_feature    709..2751
                     /label="s1 domain"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    709..4338
                     /label="COVID19 S protein soluble fraction"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             709..4338
                     /label="Translation 709-4338"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPASVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWP"
     CDS             709..4479
                     /label="Translation 709-4479"
                     /translation="MFVFLVLLPLVSSQCVNLTTRTQLPPAYTNSFTRGVYYPDKVFRSSVLHSTQDLFLPFFSNVTWFHAIHVSGTNGTKRFDNPVLPFNDGVYFASTEKSNIIRGWIFGTTLDSKTQSLLIVNNATNVVIKVCEFQFCNDPFLGVYYHKNNKSWMESEFRVYSSANNCTFEYVSQPFLMDLEGKQGNFKNLREFVFKNIDGYFKIYSKHTPINLVRDLPQGFSALEPLVDLPIGINITRFQTLLALHRSYLTPGDSSSGWTAGAAAYYVGYLQPRTFLLKYNENGTITDAVDCALDPLSETKCTLKSFTVEKGIYQTSNFRVQPTESIVRFPNITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVCGPKKSTNLVKNKCVNFNFNGLTGTGVLTESNKKFLPFQQFGRDIADTTDAVRDPQTLEILDITPCSFGGVSVITPGTNTSNQVAVLYQDVNCTEVPVAIHADQLTPTWRVYSTGSNVFQTRAGCLIGAEHVNNSYECDIPIGAGICASYQTQTNSPASVASQSIIAYTMSLGAENSVAYSNNSIAIPTNFTISVTTEILPVSMTKTSVDCTMYICGDSTECSNLLLQYGSFCTQLNRALTGIAVEQDKNTQEVFAQVKQIYKTPPIKDFGGFNFSQILPDPSKPSKRSFIEDLLFNKVTLADAGFIKQYGDCLGDIAARDLICAQKFNGLTVLPPLLTDEMIAQYTSALLAGTITSGWTFGAGAALQIPFAMQMAYRFNGIGVTQNVLYENQKLIANQFNSAIGKIQDSLSSTASALGKLQDVVNQNAQALNTLVKQLSSNFGAISSVLNDILSRLDPPEAEVQIDRLITGRLQSLQTYVTQQLIRAAEIRASANLAATKMSECVLGQSKRVDFCGKGYHLMSFPQSAPHGVVFLHVTYVPAQEKNFTTAPAICHDGKAHFPREGVFVSNGTHWFVTQRNFYEPQIITTDNTFVSGNCDVVIGIVNNTVYDPLQPELDSFKEELDKYFKNHTSPDVDLGDISGINASVVNIQKEIDRLNEVAKNLNESLIDLQELGKYEQYIKWPGGSGLVPRGSPGSGYIPEAPRDGQAYVRKDGEWVLLSTFLGHHHHHH"
     misc_feature    1663..2331
                     /label="RBD"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    2564..4544
                     /label="Gene Fragment-2 S"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2752..2754
                     /label="Furin Site to A"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2755..4338
                     /label="S2 subunit extracellular"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    3655..3657
                     /label="K-to-P"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3658..3660
                     /label="V-to-P"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4339..4350
                     /label="Short linker"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     CDS             4339..4350
                     /label="Translation 4339-4350"
                     /translation="GGSG"
     misc_feature    4351..4368
                     /label="thrombin Clevage Site"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             4351..4368
                     /label="Translation 4351-4368"
                     /translation="LVPRGS"
     misc_feature    4351..4479
                     /label="sequence from ref1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    4369..4377
                     /label="linker"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     CDS             4369..4377
                     /label="Translation 4369-4377"
                     /translation="PGS"
     misc_feature    4378..4458
                     /label="Foldon Domain"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     CDS             4378..4458
                     /label="Translation 4378-4458"
                     /translation="GYIPEAPRDGQAYVRKDGEWVLLSTFL"
     CDS             4459..4461
                     /label="Translation 4459-4461"
                     /translation="G"
     misc_feature    4462..4479
                     /label="6X his Tag"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             4462..4479
                     /label="Translation 4462-4479"
                     /translation="HHHHHH"
     CDS             4480..4482
                     /label="Translation 4480-4482"
                     /translation="*"
     CDS             4483..4485
                     /label="Translation 4483-4485"
                     /translation="*"
     misc_feature    4486..4491
                     /label="Cetuximab(gibson)"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     intron          4560..4789
                     /label="chimeric intron"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     4772..4791
                     /label="pMT2-F"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    4862..5435
                     /label="IRES"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     primer_bind     complement(5029..5046)
                     /label="IRES reverse"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     5256..5275
                     /label="IRES-F"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     CDS             5471..5473
                     /label="Translation 5471-5473"
                     /translation="M"
     CDS             5471..6274
                     /label="NeoR/KanR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(5534..5553)
                     /label="Neo-R"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     primer_bind     6144..6163
                     /label="Neo-F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             6272..6274
                     /label="Translation 6272-6274"
                     /translation="*"
     polyA_signal    6404..6525
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(6441..6460)
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     primer_bind     complement(6627..6643)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(6627..6643)
                     /label="M13 Reverse"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer_bind     complement(6640..6662)
                     /label="M13/pUC Reverse"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     protein_bind    6651..6667
                     /label="lac operator"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        complement(6675..6705)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     protein_bind    6720..6741
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     complement(6858..6875)
                     /label="L4440"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(7029..7617)
                     /label="ori"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer_bind     complement(7109..7128)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(7788..8648)
                     /label="AmpR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     8411..8430
                     /label="Amp-R"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     promoter        complement(8649..8753)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     primer_bind     complement(8828..8847)
                     /label="pRS-marker"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
ORIGIN
        1 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
       61 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
      121 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
      181 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
      241 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
      301 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
      361 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
      421 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
      481 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
      541 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
      601 ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat
      661 agggagaccc aagcttggta ccgagctcgg atcgatATCG CCGCCACCAT GTTCGTGTTC
      721 CTGGTGCTGC TGCCCCTGGT CTCCAGCCAG TGTGTGAACC TGACAACCCG GACACAGCTG
      781 CCTCCTGCCT ACACAAACAG CTTCACCCGT GGCGTCTACT ACCCCGACAA GGTCTTCCGG
      841 AGTAGCGTGC TCCACAGCAC ACAGGATCTG TTCCTGCCCT TCTTCAGCAA TGTCACCTGG
      901 TTCCACGCCA TCCACGTGAG CGGCACCAAC GGCACAAAGA GATTCGACAA CCCCGTGCTG
      961 CCTTTCAACG ACGGCGTGTA CTTCGCCAGT ACTGAGAAGA GCAACATCAT CAGAGGATGG
     1021 ATCTTCGGTA CCACCCTGGA CTCCAAAACC CAGAGCCTGC TGATCGTGAA TAATGCCACA
     1081 AACGTGGTGA TCAAAGTGTG TGAATTCCAG TTTTGTAATG ATCCATTTCT CGGCGTATAC
     1141 TACCACAAGA ACAACAAAAG CTGGATGGAA TCCGAGTTCA GAGTGTATAG CTCTGCTAAC
     1201 AACTGCACCT TCGAGTACGT GTCCCAGCCT TTCCTGATGG ACCTGGAAGG CAAGCAGGGC
     1261 AACTTCAAGA ACCTGAGAGA ATTTGTGTTC AAAAACATCG ATGGCTATTT CAAGATCTAC
     1321 AGCAAGCACA CCCCTATCAA CCTGGTGCGG GATCTTCCCC AGGGCTTCAG CGCCCTGGAG
     1381 CCTCTGGTGG ACCTGCCGAT CGGAATCAAC ATCACCAGAT TCCAGACCCT GCTGGCCCTG
     1441 CACAGATCTT ACCTGACCCC TGGCGACAGC AGCAGTGGCT GGACAGCTGG AGCCGCCGCT
     1501 TATTACGTGG GCTACCTGCA GCCTAGAACC TTCCTGCTGA AATACAACGA GAACGGAACC
     1561 ATCACCGACG CTGTTGATTG CGCTCTTGAT CCCCTGTCTG AAACCAAATG CACCCTGAAG
     1621 AGCTTTACCG TGGAAAAGGG CATATATCAG ACATCCAACT TCAGAGTGCA GCCTACAGAG
     1681 AGCATCGTGC GGTTCCCTAA TATCACGAAC CTGTGTCCTT TCGGCGAGGT GTTCAACGCC
     1741 ACCCGGTTCG CCAGCGTGTA CGCCTGGAAC CGGAAGAGGA TCAGCAATTG CGTGGCCGAT
     1801 TACAGCGTGC TGTACAACAG CGCCAGCTTC AGCACTTTCA AGTGCTACGG CGTGTCTCCT
     1861 ACCAAGCTGA ACGACCTGTG CTTCACCAAC GTGTACGCCG ACTCTTTTGT GATCAGAGGC
     1921 GACGAGGTGC GGCAGATTGC CCCTGGCCAG ACAGGAAAGA TCGCGGATTA CAACTACAAG
     1981 CTGCCTGATG ACTTCACCGG CTGCGTGATC GCCTGGAATA GCAACAACCT GGACAGCAAG
     2041 GTGGGAGGAA ACTACAACTA CCTGTACCGG CTGTTTAGAA AGTCTAACCT GAAGCCTTTC
     2101 GAGCGGGACA TCAGCACCGA GATATACCAG GCCGGCTCAA CACCTTGCAA CGGCGTGGAA
     2161 GGATTCAATT GTTACTTTCC CCTCCAATCT TACGGCTTCC AGCCAACAAA TGGCGTCGGC
     2221 TACCAGCCGT ACAGAGTCGT TGTGCTCTCT TTCGAGCTGC TGCATGCCCC TGCCACCGTG
     2281 TGCGGCCCCA AGAAATCCAC CAATCTGGTG AAGAACAAGT GCGTGAACTT TAACTTTAAC
     2341 GGACTTACCG GCACAGGCGT TCTGACGGAA AGCAACAAGA AGTTTCTGCC ATTCCAACAG
     2401 TTCGGCAGAG ACATCGCCGA CACCACAGAT GCCGTGAGGG ACCCTCAAAC CCTGGAAATA
     2461 CTCGACATCA CACCCTGTAG CTTCGGCGGA GTGAGCGTGA TCACTCCCGG CACCAACACC
     2521 TCCAACCAAG TGGCAGTGCT GTACCAGGAC GTGAACTGCA CCGAGGTCCC CGTGGCCATC
     2581 CACGCCGACC AGCTGACCCC TACCTGGAGA GTTTACTCCA CAGGGAGCAA CGTTTTCCAG
     2641 ACCCGGGCCG GCTGTCTGAT CGGCGCCGAG CACGTGAACA ATTCCTACGA GTGTGACATC
     2701 CCCATCGGCG CCGGCATTTG CGCAAGCTAC CAGACGCAGA CAAACAGCCC TGCTTCTGTG
     2761 GCTAGCCAAT CTATCATCGC CTACACCATG TCTCTCGGCG CCGAGAACAG CGTGGCTTAT
     2821 AGCAACAACA GCATCGCCAT CCCAACCAAC TTCACAATCA GCGTGACAAC CGAAATCCTG
     2881 CCCGTGAGCA TGACCAAGAC CTCCGTGGAT TGCACAATGT ACATCTGCGG CGACAGCACA
     2941 GAATGCTCTA ACCTGCTGCT GCAGTACGGC AGCTTTTGCA CCCAACTGAA CAGAGCCCTG
     3001 ACCGGCATCG CCGTGGAACA GGATAAGAAC ACACAGGAGG TGTTCGCCCA GGTGAAACAG
     3061 ATCTACAAGA CACCTCCCAT CAAAGACTTC GGCGGATTTA ACTTCTCTCA GATCCTGCCA
     3121 GACCCCAGCA AACCTAGCAA GCGGAGCTTC ATCGAGGACC TGCTGTTCAA CAAGGTGACC
     3181 CTGGCCGACG CTGGATTTAT CAAGCAGTAC GGCGATTGCC TGGGCGACAT CGCTGCCAGA
     3241 GATCTGATCT GCGCCCAGAA GTTCAATGGC CTGACAGTGC TTCCGCCTCT GCTGACAGAC
     3301 GAGATGATCG CCCAGTACAC CAGCGCTCTG CTTGCCGGCA CCATCACCAG CGGATGGACC
     3361 TTTGGAGCCG GCGCCGCCCT GCAAATCCCC TTCGCCATGC AGATGGCCTA CAGATTCAAC
     3421 GGAATCGGCG TGACCCAGAA CGTGCTGTAC GAAAACCAGA AGCTGATTGC CAATCAGTTC
     3481 AACTCCGCCA TCGGAAAAAT CCAAGACTCT CTGAGCTCCA CCGCCAGCGC CCTGGGAAAG
     3541 CTGCAAGACG TGGTCAACCA GAACGCCCAG GCCCTGAACA CCCTGGTTAA GCAGCTGAGC
     3601 TCTAACTTCG GCGCCATCAG CTCAGTGCTG AATGACATCC TCAGCAGACT GGACCCTCCC
     3661 GAGGCCGAGG TCCAGATCGA TAGACTCATC ACAGGTAGAC TGCAGAGCCT GCAAACCTAC
     3721 GTCACACAGC AGCTGATCAG AGCCGCCGAG ATCAGAGCCA GCGCCAACCT GGCTGCCACA
     3781 AAAATGAGCG AATGCGTGCT GGGCCAGAGC AAGCGCGTGG ATTTCTGCGG CAAGGGCTAC
     3841 CACCTGATGT CTTTTCCACA GAGCGCTCCC CACGGCGTGG TGTTCCTGCA CGTGACCTAC
     3901 GTGCCTGCTC AAGAGAAGAA CTTTACCACC GCCCCTGCGA TCTGTCACGA CGGCAAGGCC
     3961 CATTTCCCTC GGGAAGGCGT GTTCGTGAGC AATGGTACAC ACTGGTTCGT GACCCAAAGA
     4021 AACTTCTACG AGCCTCAGAT CATCACCACC GACAACACTT TCGTCTCCGG GAACTGCGAC
     4081 GTGGTGATCG GCATCGTGAA CAACACCGTG TACGACCCTC TGCAGCCAGA GCTGGACAGC
     4141 TTTAAGGAGG AACTGGACAA GTACTTCAAG AACCACACCA GTCCTGATGT GGACCTGGGT
     4201 GATATTAGCG GCATCAACGC CAGCGTGGTG AATATCCAGA AGGAAATCGA CAGACTGAAC
     4261 GAGGTGGCCA AGAACCTGAA CGAATCCCTG ATCGACCTGC AGGAGCTGGG TAAATACGAG
     4321 CAGTACATAA AGTGGCCTGG AGGCAGCGGT CTTGTCCCTC GGGGATCACC TGGGTCTGGC
     4381 TACATACCTG AAGCCCCACG CGATGGCCAA GCCTACGTAA GGAAAGATGG TGAATGGGTG
     4441 TTGCTTTCAA CCTTTCTCGG CCACCACCAC CATCATCATT AATAAGGATC CGCggccgca
     4501 tagataactg atccagtgtg ctggaattaa ttcgctgtct gcgagggcca gctgttgggg
     4561 tgagtactcc ctctcaaaag cgggcatgac ttctgcgcta agattgtcag tttccaaaaa
     4621 cgaggaggat ttgatattca cctggcccgc ggtgatgcct ttgagggtgg ccgcgtccat
     4681 ctggtcagaa aagacaatct ttttgttgtc aagcttgagg tgtggcaggc ttgagatctg
     4741 gccatacact tgagtgacaa tgacatccac tttgcctttc tctccacagg tgtccactcc
     4801 caggtccaac tgcaggtcga gcatgcatct agggcggcca attccgcccc tctccccccc
     4861 ccccctctcc ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
     4921 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
     4981 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
     5041 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
     5101 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
     5161 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
     5221 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
     5281 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
     5341 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
     5401 gggacgtggt tttcctttga aaaacacgat gataagcttg ccacaacccg ggataattcc
     5461 tgcagccaat atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc
     5521 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     5581 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     5641 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     5701 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     5761 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     5821 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     5881 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     5941 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     6001 caaggcgcgc atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc
     6061 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     6121 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     6181 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     6241 cgccttctat cgccttcttg acgagttctt ctgaggggat caattctcta gataactgat
     6301 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     6361 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     6421 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6481 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaacgcg tcgagtgcat
     6541 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6601 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6661 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6721 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6781 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     6841 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6901 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6961 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7021 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7081 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7141 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7201 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7261 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7321 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7381 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7441 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7501 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7561 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7621 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7681 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7741 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7801 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7861 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7921 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7981 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8041 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8101 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8161 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8221 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8281 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     8341 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     8401 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     8461 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     8521 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8581 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8641 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8701 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8761 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     8821 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     8881 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     8941 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     9001 gg
//