LOCUS       pR_Lux                  9598 bp    DNA     circular SYN 30-AUG-2024
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     Annotated with pLannotate v1.2.0.
FEATURES             Location/Qualifiers
     rep_origin      join(complement(1..310),complement(9363..9598))
     CDS             complement(465..1178)
                     /note="pLannotate"
                     /standard_name="λ repressor"
     CDS             1279..2721
                     /note="pLannotate"
                     /standard_name="LuxC"
     CDS             2734..3657
                     /note="pLannotate"
                     /standard_name="LuxD"
     CDS             3706..4788
                     /note="pLannotate"
                     /standard_name="LuxA"
     CDS             4803..5786
                     /note="pLannotate"
                     /standard_name="LuxB"
     CDS             5965..7077
                     /note="pLannotate"
                     /standard_name="LuxE"
     regulatory      7110..7196
                     /note="Geneious type: terminator"
                     /note="pLannotate"
                     /standard_name="rrnB T1 terminator"
     oriT            7757..7866
                     /note="pLannotate"
                     /standard_name="oriT"
     CDS             7899..7963
                     /note="pLannotate"
                     /standard_name="traJ (fragment)"
     CDS             complement(7968..8072)
                     /note="pLannotate"
                     /standard_name="TRA2_ECOLX (fragment)"
     CDS             complement(8210..9025)
                     /note="pLannotate"
                     /standard_name="KanR"
     rep_origin      complement(9220..9275)
ORIGIN      
        1 atgcacgaac cccccgttca gtccgaccgc tgcgccttat ccggtaacta tcgtcttgag
       61 tccaacccgg aaagacatgc aaaagcacca ctggcagcag ccactggtaa ttgatttaga
      121 ggagttagtc ttgaagtcat gcgccggtta aggctaaact gaaaggacaa gttttggtga
      181 ctgcgctcct ccaagccagt tacctcggtt caaagagttg gtagctcaga gaaccttcga
      241 aaaaccgccc tgcaaggcgg ttttttcgtt ttcagagcaa gagattacgc gcagaccaaa
      301 acgatctcaa gaagatcatc ttattaatca gataatatat ttttagcgga gtgactataa
      361 aaaggcgcac tgatcaatgc gcctttttta taagatcata aaagaccctt catttatgaa
      421 gggtcttttt tgcatgcggc gggtaccgct cgagttaatt aagatcagcc aaacgtctct
      481 tcaggccact gactagcgat aactttcccc acaacggaac aactctcatt gcatgggatc
      541 attgggtact gtgggtttag tggttgtaaa aacacctgac cgctatccct gatcagtttc
      601 ttgaaggtaa actcatcacc cccaagtctg gctatgcaga aatcacctgg ctcaacagcc
      661 tgctcagggt caacgagaat taacattccg tcaggaaagc ttggcttgga gcctgttggt
      721 gcggtcatgg aattaccttc aacctcaagc cagaatgcag aatcactggc ttttttggtt
      781 gtgcttaccc atctctccgc atcacctttg gtaaaggttc taagctcagg tgagaacatc
      841 cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga cggctgcata
      901 ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa ttcttcaacg
      961 ctaactttga gaattcttgc aagcaatgcg gcgttataag catttaatgc attgatgcca
     1021 ttaaataaag caccaacgcc tgactgcccc atccccatct tgtctgcgac agattcctgg
     1081 gataagccaa gttcattttt ctttttttca taaattgctt taaggcgacg tgcgtcctca
     1141 agctgctctt gtgttaatgg tttctttttt gtgctcatac gttaaatcta tcaccgcaag
     1201 ggataaatat ctaacaccgt gcgtgttgac tattttacct ctggcggtga taatggttgc
     1261 atgtactaag gaggttgtat gactaaaaaa atttcattca ttattaacgg ccaggttgaa
     1321 atctttcccg aaagtgatga tttagtgcaa tccattaatt ttggtgataa tagtgtttac
     1381 ctgccaatat tgaatgactc tcatgtaaaa aacattattg attgtaatgg aaataacgaa
     1441 ttacggttgc ataacattgt caattttctc tatacggtag ggcaaagatg gaaaaatgaa
     1501 gaatactcaa gacgcaggac atacattcgt gacttaaaaa aatatatggg atattcagaa
     1561 gaaatggcta agctagaggc caattggata tctatgattt tatgttctaa aggcggcctt
     1621 tatgatgttg tagaaaatga acttggttct cgccatatca tggatgaatg gctacctcag
     1681 gatgaaagtt atgttcgggc ttttccgaaa ggtaaatctg tacatctgtt ggcaggtaat
     1741 gttccattat ctgggatcat gtctatatta cgcgcaattt taactaagaa tcagtgtatt
     1801 ataaaaacat cgtcaaccga tccttttacc gctaatgcat tagcgttaag ttttattgat
     1861 gtagacccta atcatccgat aacgcgctct ttatctgtta tatattggcc ccaccaaggt
     1921 gatacatcac tcgcaaaaga aattatgcga catgcggatg ttattgtcgc ttggggaggg
     1981 ccagatgcga ttaattgggc ggtagagcat gcgccatctt atgctgatgt gattaaattt
     2041 ggttctaaaa agagtctttg cattatcgat aatcctgttg atttgacgtc cgcagcgaca
     2101 ggtgcggctc atgatgtttg tttttacgat cagcgagctt gtttttctgc ccaaaacata
     2161 tattacatgg gaaatcatta tgaggaattt aagttagcgt tgatagaaaa acttaatcta
     2221 tatgcgcata tattaccgaa tgccaaaaaa gattttgatg aaaaggcggc ctattcttta
     2281 gttcaaaaag aaagcttgtt tgctggatta aaagtagagg tggatattca tcaacgttgg
     2341 atgattattg agtcaaatgc aggtgtggaa tttaatcaac cacttggcag atgtgtgtac
     2401 cttcatcacg tcgataatat tgagcaaata ttgccttatg ttcaaaaaaa taagacgcaa
     2461 accatatcta tttttccttg ggagtcatca tttaaatatc gagatgcgtt agcattaaaa
     2521 ggtgcggaaa ggattgtaga agcaggaatg aataacatat ttcgagttgg tggatctcat
     2581 gacggaatga gaccgttgca acgattagtg acatatattt ctcatgaaag gccatctaac
     2641 tatacggcta aggatgttgc ggttgaaata gaacagactc gattcctgga agaagataag
     2701 ttccttgtat ttgtcccata ataggtaaaa agtatggaaa atgaatcaaa atataaaacc
     2761 atcgaccacg ttatttgtgt tgaaggaaat aaaaaaattc atgtttggga aacgctgcca
     2821 gaagaaaaca gcccaaagag aaagaatgcc attattattg cgtctggttt tgcccgcagg
     2881 atggatcatt ttgctggtct ggcggaatat ttatcgcgga atggatttca tgtgatccgc
     2941 tatgattcgc ttcaccacgt tggattgagt tcagggacaa ttgatgaatt tacaatgtct
     3001 ataggaaagc agagcttgtt agcagtggtt gattggttaa ctacacgaaa aataaataac
     3061 ttcggtatgt tggcttcaag cttatctgcg cggatagctt atgcaagcct atctgaaatc
     3121 aatgcttcgt ttttaatcac cgcagtcggt gttgttaact taagatattc tcttgaaaga
     3181 gctttagggt ttgattatct cagtctaccc attaatgaat tgccgaataa tctagatttt
     3241 gaaggccata aattgggtgc tgaagtcttt gcgagagatt gtcttgattt tggttgggaa
     3301 gatttagctt ctacaattaa taacatgatg tatcttgata taccgtttat tgcttttact
     3361 gcaaataacg ataattgggt caagcaagat gaagttatca cattgttatc aaatattcgt
     3421 agtaatcgat gcaagatata ttctttgtta ggaagttcgc atgacttgag tgaaaattta
     3481 gtggtcctgc gcaattttta tcaatcggtt acgaaagccg ctatcgcgat ggataatgat
     3541 catctggata ttgatgttga tattactgaa ccgtcatttg aacatttaac tattgcgaca
     3601 gtcaatgaac gccgaatgag aattgagatt gaaaatcaag caatttctct gtcttaaaat
     3661 ctattgagat attctatcac tcaaatagca atataaggac tctctatgaa atttggaaac
     3721 tttttgctta cataccaacc tccccaattt tctcaaacag aggtaatgaa acgtttggtt
     3781 aaattaggtc gcatctctga ggagtgtggt tttgataccg tatggttact ggagcatcat
     3841 ttcacggagt ttggtttgct tggtaaccct tatgtcgctg ctgcatattt acttggcgcg
     3901 actaaaaaat tgaatgtagg aactgccgct attgttcttc ccacagccca tccagtacgc
     3961 caacttgaag atgtgaattt attggatcaa atgtcaaaag gacgatttcg gtttggtatt
     4021 tgccgagggc tttacaacaa ggactttcgc gtattcggca cagatatgaa taacagtcgc
     4081 gccttagcgg aatgctggta cgggctgata aagaatggca tgacagaggg atatatggaa
     4141 gctgataatg aacatatcaa gttccataag gtaaaagtaa accccgcggc gtatagcaga
     4201 ggtggcgcac cggtttatgt ggtggctgaa tcagcttcga cgactgagtg ggctgctcaa
     4261 tttggcctac cgatgatatt aagttggatt ataaatacta acgaaaagaa agcacaactt
     4321 gagctttata atgaagtggc tcaagaatat gggcacgata ttcataatat cgaccattgc
     4381 ttatcatata taacatctgt agatcatgac tcaattaaag cgaaagagat ttgccggaaa
     4441 tttctggggc attggtatga ttcttatgtg aatgctacga ctatttttga tgattcagac
     4501 caaacaagag gttatgattt caataaaggg cagtggcgtg actttgtatt aaaaggacat
     4561 aaagatacta atcgccgtat tgattacagt tacgaaatca atcccgtggg aacgccgcag
     4621 gaatgtattg acataattca aaaagacatt gatgctacag gaatatcaaa tatttgttgt
     4681 ggatttgaag ctaatggaac agtagacgaa attattgctt ccatgaagct cttccagtct
     4741 gatgtcatgc catttcttaa agaaaaacaa cgttcgctat tatattagct aaggagaaag
     4801 aaatgaaatt tggattgttc ttccttaact tcatcaattc aacaactgtt caagaacaaa
     4861 gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt gaacagattt
     4921 tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg actgtttctg
     4981 gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac atcattacaa
     5041 ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta agtgaaggga
     5101 gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt tttaatcgcc
     5161 cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac gatgctttaa
     5221 caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata tctgtaaatc
     5281 cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt catcatattg
     5341 ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat tctaatgatg
     5401 ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac gttgacctat
     5461 cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt aataaagcta
     5521 aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct aatgaaaatt
     5581 tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat acggagtgta
     5641 taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg ctgtcctttg
     5701 aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa tattgttgat gataatatta
     5761 agaagtacca catggaatat acctaataga tttcgagttg cagcgaggcg gcaagtgaac
     5821 gaatccccag gagcatagat aactatgtga ctggggtgag tgaaagcagc caacaaagca
     5881 gcagcttgaa agatgaaggg tataaaagag tatgacagca gtgctgccat actttctaat
     5941 attatcttga ggagtaaaac aggtatgact tcatatgttg ataaacaaga aattacagca
     6001 agctcagaaa ttgatgattt gattttttcg agcgatccat tagtgtggtc ttacgacgag
     6061 caggaaaaaa tcagaaagaa acttgtgctt gatgcatttc gtaatcatta taaacattgt
     6121 cgagaatatc gtcactactg tcaggcacac aaagtagatg acaatattac ggaaattgat
     6181 gacatacctg tattcccaac atcggttttt aagtttactc gcttattaac ttctcaggaa
     6241 aacgagattg aaagttggtt taccagtagc ggcacgaatg gtttaaaaag tcaggtggcg
     6301 cgtgacagat taagtattga gagactctta ggctctgtga gttatggcat gaaatatgtt
     6361 ggtagttggt ttgatcatca aatagaatta gtcaatttgg gaccagatag atttaatgct
     6421 cataatattt ggtttaaata tgttatgagt ttggtggaat tgttatatcc tacgacattt
     6481 accgtaacag aagaacgaat agattttgtt aaaacattga atagtcttga acgaataaaa
     6541 aatcaaggga aagatctttg tcttattggt tcgccatact ttatttattt actctgccat
     6601 tatatgaaag ataaaaaaat ttcattttct ggagataaaa gcctttatat cataaccgga
     6661 ggcggctgga aaagttacga aaaagaatct ctgaaacgtg atgatttcaa tcatctttta
     6721 tttgatactt tcaatctcag tgatattagt cagatccgag atatatttaa tcaagttgaa
     6781 ctcaacactt gtttctttga ggatgaaatg cagcgtaaac atgttccgcc gtgggtatat
     6841 gcgcgagcgc ttgatcctga aacgttgaaa cctgtacctg atggaacgcc ggggttgatg
     6901 agttatatgg atgcgtcagc aaccagttat ccagcattta ttgttaccga tgatgtcggg
     6961 ataattagca gagaatatgg taagtatccc ggcgtgctcg ttgaaatttt acgtcgcgtc
     7021 aatacgagga cgcagaaagg gtgtgcttta agcttaaccg aagcgtttga tagttgatat
     7081 cctttgcctg cggccgcaac tagaggcatc aaataaaacg aaaggctcag tcgaaagact
     7141 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc
     7201 cgccctagac ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     7261 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     7321 tccagaattg ccatgatttt tggatccggt gattgattga gcaagcttta tgcttgtaaa
     7381 ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc caattactag
     7441 ccaattcgat tactggctta ctatgttggc actgatgagg gtgtcagtga agtgacctgc
     7501 aggggggggg gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggctt
     7561 aattcctgca tttgcctgtt tccagtcggt agatattcca caaaacagca gggaagcagc
     7621 gcttttccgc tgcataaccc tgcttcgggg tcattatagc gattttttcg gtatatccat
     7681 cctttttcgc acgatataca ggattttgcc aaagggttcg tgtagacttt ccttggtgta
     7741 tccaacggcg tcagccgggc aggataggtg aagtaggccc acccgcgagc gggtgttcct
     7801 tcttcactgt cccttattcg cacctggcgg tgctcaacgg gaatcctgct ctgcgaggct
     7861 ggccggctac cgccggcgta acagatgagg gcaagcggat ggctgatgaa accaagccaa
     7921 ccaggaaggg cagcccacct atcaaggtgt actgccttcc agaattaagc ctgaatcgcc
     7981 ccatcatcca gccagaaagt gagggagcca cggttgatga gagctttgtt gtaggtggac
     8041 cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc gggaagatgc
     8101 gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg ccgtcccgtc
     8161 aagtcagcgt aatgctctgc cagtgttaca accaattaac caattctgat tagaaaaact
     8221 catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt
     8281 gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa
     8341 gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc
     8401 cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg
     8461 agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag ccattacgct
     8521 cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga
     8581 gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc
     8641 gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata
     8701 cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac
     8761 ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca
     8821 tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg
     8881 catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag
     8941 cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc ctcgagcaag
     9001 acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca
     9061 gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag agattttgag
     9121 acacaacgtg gctttccccc ccccccctgc aggtccttca tgtggcagga gaaaaaaggc
     9181 tgcaccggtg cgtcagcaga atatgtgata caggatatat tccgcttcct cgctcactga
     9241 ctcgctacgc tcggtcgttc gactgcggcg agcggaaatg gcttacgaac ggggcggaga
     9301 tttcctggaa gatgccagga agatacttaa cagggaagtg agagggccgc ggcaaagccg
     9361 tttttccata ggctccgccc ccctgacaag catcacgaaa tctgacgctc aaatcagtgg
     9421 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggcgg ctccctcgtg
     9481 cgctctcctg ttcctgcctt tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg
     9541 tctcattcca cgcctgacac tcagttccgg gtaggcagtt cgctccaagc tggactgt
//
