LOCUS       pAAV_mIba1(1700)        7651 bp DNA     circular SYN 02-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7651)
  AUTHORS   MolecularRoom
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7651
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     misc_feature    50..235
                     /label=ITR
                     /note="color: #cc0000; direction: RIGHT"
     promoter        242..1919
                     /gene="mIba1 promoter"
                     /label=mIba1 promoter
                     /note="color: #ff00ff; direction: RIGHT"
     CDS             1933..2652
                     /codon_start=1
                     /gene="EGFP"
                     /label=EGFP
                     /note="color: #00ff00"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2063..2083)
                     /label=#165(Matsu) 3-EGFP
                     /note="color: 黒; sequence: TGGTGCAGATGAACTTCAGGG; added: 
                     2023-03-06"
     primer_bind     2645..2695
                     /label=#1257 EGFP_miR-9/129T_notⅠ_InF-F
                     /note="color: 黒; sequence: 
                     acaagtaaagcggccgctcatacagctagataaccaaagcgattcatacag; added:
                     2023-02-24"
     CDS             2662..2763
                     /gene="miR-9-Tx4"
                     /label=miR-9-Tx4
                     /note="color: #ffff00; direction: RIGHT"
     CDS             2662..2683
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             2688..2709
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             2716..2737
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             2742..2763
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             2770..2871
                     /gene="miR129-2-3p.Tx4"
                     /label=miR129-2-3p.Tx4
                     /note="color: #00ffff; direction: RIGHT"
     CDS             2770..2791
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     CDS             2796..2817
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     CDS             2824..2845
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     primer_bind     complement(2848..2886)
                     /label=#1258 EGFP_miR-9/129T_notⅠ_InF-R
                     /note="color: 黒; sequence: 
                     attatcgatgcggccgcaagcccttaccccaaaaagcatgt; added: 
                     2023-02-24"
     primer_bind     complement(2848..2886)
                     /label=#1330 EGFP_miR-9/129T_InF-R
                     /note="color: 黒; sequence: 
                     attatcgatgcggccaagcccttaccccaaaaagcatgt; added: 2023-06-02"
     CDS             2850..2871
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     misc_feature    2880..3474
                     /gene="WPRE"
                     /label=WPRE
                     /note="color: #800080"
     primer_bind     complement(2976..3001)
                     /label=#195(Matsu) seq-WPRE(R)New
                     /note="color: 黒; sequence: GGGAAGCAATAGCATGATACAAAGGC; 
                     added: 2023-03-06"
     primer_bind     3493..3527
                     /label=#1257 EGFP_miR-9/129T_notⅠ_InF-F
                     /note="color: 黒; sequence: 
                     acaagtaaagcggccgctcatacagctagataaccaaagcgattcatacag; added:
                     2023-02-24"
     CDS             3494..3595
                     /gene="miR-9-Tx4"
                     /label=miR-9-Tx4
                     /note="color: #ffff00; direction: RIGHT"
     CDS             3494..3515
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             3520..3541
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             3548..3569
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             3574..3595
                     /gene="miR-9-T"
                     /label=miR-9-T
                     /note="color: #ffff99; direction: RIGHT"
     CDS             3602..3703
                     /gene="miR129-2-3p.Tx4"
                     /label=miR129-2-3p.Tx4
                     /note="color: #00ffff; direction: RIGHT"
     CDS             3602..3623
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     CDS             3628..3649
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     CDS             3656..3677
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     primer_bind     complement(3680..3705)
                     /label=#1330 EGFP_miR-9/129T_InF-R
                     /note="color: 黒; sequence: 
                     attatcgatgcggccaagcccttaccccaaaaagcatgt; added: 2023-06-02"
     primer_bind     complement(3680..3704)
                     /label=#1258 EGFP_miR-9/129T_notⅠ_InF-R
                     /note="color: 黒; sequence: 
                     attatcgatgcggccgcaagcccttaccccaaaaagcatgt; added: 
                     2023-02-24"
     CDS             3682..3703
                     /codon_start=1
                     /gene="miR-129-2-3p.T"
                     /label=miR-129-2-3p.T
                     /note="color: #ccffff"
                     /translation="MLFGVRA"
     polyA_signal    3731..3859
                     /gene="SV40pA"
                     /label=SV40pA
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    3883..4068
                     /label=ITR
                     /note="color: #cc0000; direction: RIGHT"
     rep_origin      complement(4551..5064)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     promoter        5743..5847
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     CDS             join(5848..5916,5917..6708)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 5848 .. 5916 / #ccffcc / signal sequence
                        2: 5917 .. 6708 / #ccffcc
                     Cleavage site after base 5916"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6879..7467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
ORIGIN
        1 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctgcgcgc
       61 tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct ttggtcgccc
      121 ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca ctaggggttc
      181 cttgtagtta atgattaacc cgccatgcta cttatctacg tagccatgct ctagactcga
      241 gtactatagg atgcatcgtg aaaacctcgt ttccaccagg aactgaggtt gctgctggag
      301 gaaatctccc atgaagagct gttaactggg gaactggttg gatccagctc ctacaagctc
      361 tcctttaatt tccatacaca tgccaagctg tgaacacctg cacacacagt acaaataaaa
      421 cagtagaaat gaaatgaaaa ttaaaacaaa aacagcatac tttctccttt ctacccacat
      481 atctcctgca tccctgagag caatttcttg gccatcttcc ataatcatgc tattcaaacc
      541 tgccctctaa gtacagagct ctgaatggag accacgggag cagacttttc atctgttggc
      601 tccctgggcc tagcagtgta ccctgcaaac aggaaacctt cgctcatcat ttatcacatg
      661 aatgtggcta gagaactgtc cccaccagac actgagagcc tctcacaggg ccagagggtg
      721 gctgcctcaa aggcaggcga gaggctgcag tcttctgcag agtggacaga tactgcctgc
      781 catacaaggg gacatagcct ggggcggggg gcacaaggga aggggcctga gtgcattgga
      841 gtcacagccc tccctcccct ggtgacccag tgactgaggg gaccaaggct atccctggta
      901 tgaggggagg gcatttgcat gagttagacc cctgacagcc catggtgggg acaggaagta
      961 gctctgtcta ctgtctcttt cagtctcact ttctgttcct ccaaagtgtc tcttcaaact
     1021 ctcaaatgac tttgtagatt cagcctaaga tctcaaagca ggtgaggcag gaggctggca
     1081 ctcaagagcc tgacgaacac aaacccaagt ccttctggga catgtctcca tgtgtcccca
     1141 gtgctcctct gtagcccctg agagaggttt ctttcctttc cagggcgcta ggctcagctc
     1201 accccattcc tggagcagcc tgcagacttc atcctctctc ttccatcccg gggaaagtca
     1261 gccagtcctc ctcagctgcc tgtcttaacc tgcatcatga agcctgagga gatttcaagt
     1321 aaaccctccc aagccccacc tcaggatctg gggaaagcca ctgtctaccg catccttggt
     1381 ttgagacagg ttcttactgt gttggcctgg ctgggttcca actcccagga tcctctagcc
     1441 tcagcctctc ttaggtgcta gattgcaggc ctagggtatc acgctcggtc cctcagatcc
     1501 tggtgccttt ccacacctcc gggagctgat ctaagtcttt ctcccacgta cagtctgctt
     1561 ctttggagga gctgtgtgga gctaggtgtg ttacagggct gtagctccag ccatctggga
     1621 ggctgaggca ggatggctac ttcaattctc aagagcctgg ataggagacc ctgcccccac
     1681 cccaccccca aagtaatttt tcagcacatt acttcttcat ctcctctctc aacccgttct
     1741 gccttctcct ggggtgctgg tgtcagcaga agctgatgtg gaagtgatgc ctgggagtta
     1801 gcaagggaat gagtggaaag gggaagtgtg agaacggtcc cagaagagac tggggagctg
     1861 gtggagagag gacccagcgg acagactgcc agcctaagac aaccagcgtc tgaggagcca
     1921 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     1981 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     2041 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     2101 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc
     2161 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     2221 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     2281 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     2341 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac
     2401 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc
     2461 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     2521 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     2581 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     2641 ctgtacaagt aaagcggccg ctcatacagc tagataacca aagcgattca tacagctaga
     2701 taaccaaagc aattgtcata cagctagata accaaagtca ctcatacagc tagataacca
     2761 aaggctagca tgctttttgg ggtaagggct tcgatatgct ttttggggta agggcttctt
     2821 aagatgcttt ttggggtaag ggctttcaca tgctttttgg ggtaagggct tggccgcatc
     2881 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2941 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3001 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3061 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3121 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3181 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3241 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     3301 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3361 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3421 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatgc
     3481 cgtatacggt acctcataca gctagataac caaagcgatt catacagcta gataaccaaa
     3541 gcaattgtca tacagctaga taaccaaagt cactcataca gctagataac caaaggctag
     3601 catgcttttt ggggtaaggg cttcgatatg ctttttgggg taagggcttc ttaagatgct
     3661 ttttggggta agggctttca catgcttttt ggggtaaggg cttggatcct ctagtagagt
     3721 cgaggaattc acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3781 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3841 aatgtatctt atcatgtctg tcgaccccca acgcgttgga gctctagagc atggctacgt
     3901 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     3961 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     4021 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagctggc gtaatagcga
     4081 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggaantt
     4141 ccaacatcca ataaatcata caggcaaggc aaagaattag caaaattaag caataaagcc
     4201 tcagagcata aagctaaatc ggttgtacca aaaacattat gaccctgtaa tacttttgcg
     4261 ggagaagcct ttatttcaac gcaaggataa aaatttttag aaccctcata tattttaaat
     4321 gcaatgcctg agtaatgtgt aggtaaagat tcaaacgggt gagaaaggcc ggagacagtc
     4381 aaatcaccat caatatgata ttcaaccgtt ctagctgata aattcatgcc ggagagggta
     4441 gctatttttg agaggtctct acaaaggcta tcaggtcatt gcctgagagt ctggagcaaa
     4501 caagagaatc gatgaacggt aatcgtaaaa ctagcatgtc aatcatatgt accccggttg
     4561 ataatcagaa aagccccaaa aacaggaaga ttgtataagc aaatatttaa attgtaaacg
     4621 ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4681 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4741 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4801 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4861 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4921 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4981 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     5041 ttaatgcgcc gctacagggc gcgtactatg gttgctttga cgagcacgta taacgtgctt
     5101 tcctcgttag aatcagagcg ggagctaaac aggaggccga ttaaagggat tttagacagg
     5161 aacggtacgc cagaatcctg agaagtgttt ttataatcag tgaggccacc gagtaaaaga
     5221 gtctgtccat cacgcaaatt aaccgttgtc gcaatacttc tttgattagt aataacatca
     5281 cttgcctgag tagaagaact caaactatcg gccttgctgg taatatccag aacaatatta
     5341 ccgccagcca ttgcaacagg aaaaacgctc atggaaatac ctacattttg acgctcaatc
     5401 gtctggaant tcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     5461 atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac
     5521 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5581 caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     5641 cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata
     5701 atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     5761 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     5821 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     5881 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     5941 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     6001 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     6061 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     6121 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     6181 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     6241 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     6301 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     6361 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     6421 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     6481 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     6541 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     6601 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     6661 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     6721 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     6781 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7021 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7621 agcgagtcag tgagcgagga agcggaagag c
//
