LOCUS       pCyCBE_(final)         14456 bp ds-DNA     circular     16-AUG-2024
DEFINITION  Bacterial genome editing plasmid with spectinomycin resistance
            marker and using sacB as a counter-selection marker.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
COMMENT     Imported using the Genbank importer. File name: pCpf1b-sp
            addgene122188.gbk
FEATURES             Location/Qualifiers
     misc_feature    61..929
                     /label="Km扩增片段"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             106..921
                     /label="KmR"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             complement(1147..2568)
                     /label="SacB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             complement(1147..2568)
                     /label="Translation 2568-1147"
                     /translation="MNIKKFAKQATVLTFTTALLAGGATQAFAKETNQKPYKETYGISHITRHDMLQIPEQQKNEKYQVPEFDSSTIKNISSAKGLDVWDSWPLQNADGTVANYHGYHIVFALAGDPKNADDTSIYMFYQKVGETSIDSWKNAGRVFKDSDKFDANDSILKDQTQEWSGSATFTSDGKIRLFYTDFSGKHYGKQTLTTAQVNVSASDSSLNINGVEDYKSIFDGDGKTYQNVQQFIDEGNYSSGDNHTLRDPHYVEDKGHKYLVFEANTGTEDGYQGEESLFNKAYYGKSTSFFRQESQKLLQSDKKRTAELANGALGMIELNDDYTLKKVMKPLIASNTVTDEIERANVFKMNGKWYLFTDSRGSKMTIDGITSNDIYMLGYVSNSLTGPYKPLNKTGLVLKMDLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFYADKQSTFAPSFLLNIKGKKTSVVKDSILEQGQLTVNK*"
     misc_feature    2948..3112
                     /label="rpsLSA"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    3113..3799
                     /label="APOBEC"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     CDS             3113..3799
                     /label="Translation 3113-3799"
                     /translation="MSSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWGGRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEFLSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSPSNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLPPHILWATGLK"
     misc_feature    3800..3847
                     /label="XTEN"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     CDS             3800..3847
                     /label="Translation 3800-3847"
                     /translation="SGSETPGTSESATPES"
     misc_feature    3848..7951
                     /label="cas9D10A"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             3848..7951
                     /label="Translation 3848-7951"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD*"
     snRNA           7996..8130
                     /label="sgRNA"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    8031..8047
                     /label="spacer insert site"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     source          8227..14456
                     /label="source"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /note="organism: synthetic DNA construct mol_type: other DNA"
     rep_origin      9005..9399
                     /label="RSF1010 oriV"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /note="note: replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)"
     oriT            9741..9828
                     /label="RSF1010 oriT"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)"
     CDS             11067..12038
                     /label="RSF1010 RepB"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="gene: repB product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) translation: MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             11067..12038
                     /label="RSF1010 RepB"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="gene: repB product: replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) translation: MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM*"
     CDS             12549..13388
                     /label="RSF1010 RepA"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: repA product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) translation: MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             12549..13388
                     /label="RSF1010 RepA"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="gene: repA product: replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989) translation: MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVADGLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRMEAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEEWGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA*"
ORIGIN
        1 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
       61 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa
      121 cgggaaacgt cttgctctag gccgcgatta aattccaaca tggatgctga tttatatggg
      181 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg
      241 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt
      301 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag
      361 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca
      421 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca
      481 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc
      541 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat
      601 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt
      661 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt
      721 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga
      781 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa
      841 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg
      901 atgctcgatg agtttttcta agaattaatt catgagcgga tacatatttg aatgtattta
      961 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgtgtaaaa
     1021 cgacggccag tgaattgacg cgtattggga tggtaccggg ccccccctcg aggtcgacgg
     1081 tatcgatacg gcatcagagc agattgtact gagagtgcac cataatcggc attttctttt
     1141 gcgtttttat ttgttaactg ttaattgtcc ttgttcaagg atgctgtctt tgacaacaga
     1201 tgttttcttg cctttgatgt tcagcaggaa gctaggcgca aacgttgatt gtttgtctgc
     1261 gtagaatcct ctgtttgtca tatagcttgt aatcacgaca ttgtttcctt tcgcttgagg
     1321 tacagcgaag tgtgagtaag taaaggttac atcgttagga tcaagatcca tttttaacac
     1381 aaggccagtt ttgttcagcg gcttgtatgg gccagttaaa gaattagaaa cataaccaag
     1441 catgtaaata tcgttagacg taatgccgtc aatcgtcatt tttgatccgc gggagtcagt
     1501 gaacagatac catttgccgt tcattttaaa gacgttcgcg cgttcaattt catctgttac
     1561 tgtgttagat gcaatcagcg gtttcatcac ttttttcagt gtgtaatcat cgtttagctc
     1621 aatcataccg agagcgccgt ttgctaactc agccgtgcgt tttttatcgc tttgcagaag
     1681 tttttgactt tcttgacgga agaatgatgt gcttttgcca tagtatgctt tgttaaataa
     1741 agattcttcg ccttggtagc catcttcagt tccagtgttt gcttcaaata ctaagtattt
     1801 gtggccttta tcttctacgt agtgaggatc tctcagcgta tggttgtcgc ctgagctgta
     1861 gttgccttca tcgatgaact gctgtacatt ttgatacgtt tttccgtcac cgtcaaagat
     1921 tgatttataa tcctctacac cgttgatgtt caaagagctg tctgatgctg atacgttaac
     1981 ttgtgcagtt gtcagtgttt gtttgccgta atgtttaccg gagaaatcag tgtagaataa
     2041 acggattttt ccgtcagatg taaatgtggc tgaacctgac cattcttgtg tttggtcttt
     2101 taggatagaa tcatttgcat cgaatttgtc gctgtcttta aagacgcggc cagcgttttt
     2161 ccagctgtca atagaagttt cgccgacttt ttgatagaac atgtaaatcg atgtgtcatc
     2221 cgcattttta ggatctccgg ctaatgcaaa gacgatgtgg tagccgtgat agtttgcgac
     2281 agtgccgtca gcgttttgta atggccagct gtcccaaacg tccaggcctt ttgcagaaga
     2341 gatattttta attgtggacg aatcgaactc aggaacttga tatttttcat ttttttgctg
     2401 ttcagggatt tgcagcatat catggcgtgt aatatgggaa atgccgtatg tttccttata
     2461 tggcttttgg ttcgtttctt tcgcaaacgc ttgagttgcg cctcctgcca gcagtgcggt
     2521 agtaaaggtt aatactgttg cttgttttgc aaactttttg atgttcatcg ttcatgtctc
     2581 cttttttatg tactgtgtta gcggtctgct tcttccagcc ctcctgtttg aagatggcaa
     2641 gttagttacg cacaataaaa aaagacctaa aatatgtaag gggtgacgcc aaagtataca
     2701 ctttgccctt tacacatttt aggtcttgcc tgctttatca gtaacaaacc cgcgcgattt
     2761 acttttcgac ctcattctat tagactctcg tttggattgc aactggtcta ttttcctctt
     2821 ttgtttgata gaaaatcata aaaggatttg cagactacgg gcctaaagaa ctaaaaaatc
     2881 tatctgtttc ttttcattct ctgtattttt tatagtttct gttgcatggg cataaagttg
     2941 caagcttgca aacatgcttt gggtaaacat gtaggtatta acgtcaatgc gacaatagta
     3001 gcattgatta aatgagaatt agtaagtgtt ttacttacta aattttattt aacctaaaaa
     3061 tgaaccacct ggatgtgtgg gattaaaaag tgaagagagg aggacatatc acatgagtag
     3121 cgaaaccggt ccggttgcag tggatccgac cctgcgccgt cgcattgaac cgcacgagtt
     3181 tgaagtgttc tttgatccgc gcgagctgcg caaagaaact tgcctgctgt acgagattaa
     3241 ctggggtggc cgccatagca tctggcgcca taccagccag aacaccaata agcacgtgga
     3301 agtgaatttt attgaaaaat ttaccaccga gcgctacttc tgccctaata cccgctgcag
     3361 catcacctgg tttctgagct ggagcccgtg cggcgaatgt agtcgcgcca ttaccgagtt
     3421 cctgagccgc tatccgcatg tgaccctgtt catctacatc gcccgtctgt accatcacgc
     3481 cgatccgcgc aatcgccaag gtctgcgtga tctgattagc agcggtgtga ccatccagat
     3541 catgaccgaa caagagagcg gctattgctg gcgcaacttc gtgaactatt ctccgagcaa
     3601 cgaagcccac tggccgcgtt atccgcatct gtgggtgcgc ctgtatgtgc tggagctgta
     3661 ctgcatcatc ctgggcctgc cgccttgcct gaatattctg cgccgtaaac agccgcaact
     3721 gacattcttc accatcgcac tgcagagctg ccattatcag cgcctgccgc cgcacatttt
     3781 atgggccacc ggtctgaaaa gcggtagtga aactccgggc acaagcgaaa gcgcaacccc
     3841 ggaaagtgat aagaaatact caataggctt agctatcggc acaaatagcg tcggatgggc
     3901 ggtgatcact gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga
     3961 ccgccacagt atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc
     4021 ggaagcgact cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat
     4081 ttgttatcta caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca
     4141 tcgacttgaa gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt
     4201 tggaaatata gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg
     4261 aaaaaaattg gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc
     4321 gcatatgatt aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag
     4381 tgatgtggac aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa
     4441 ccctattaac gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc
     4501 aagacgatta gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg
     4561 gaatctcatt gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc
     4621 agaagatgct aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt
     4681 ggcgcaaatt ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc
     4741 tattttactt tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc
     4801 ttcaatgatt aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt
     4861 tcgacaacaa cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata
     4921 tgcaggttat attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat
     4981 tttagaaaaa atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct
     5041 gcgcaagcaa cggacctttg acaacggctc tattccccat caaattcact tgggtgagct
     5101 gcatgctatt ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa
     5161 gattgaaaaa atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa
     5221 tagtcgtttt gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga
     5281 agaagttgtc gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga
     5341 taaaaatctt ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac
     5401 ggtttataac gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt
     5461 tctttcaggt gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt
     5521 aaccgttaag caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga
     5581 aatttcagga gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa
     5641 aattattaaa gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat
     5701 tgttttaaca ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata
     5761 tgctcacctc tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg
     5821 gggacgtttg tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat
     5881 attagatttt ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga
     5941 tgatagtttg acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag
     6001 tttacatgaa catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca
     6061 gactgtaaaa gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat
     6121 cgttattgaa atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga
     6181 gcgtatgaaa cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca
     6241 tcctgttgaa aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg
     6301 aagagacatg tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga
     6361 tcacattgtt ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg
     6421 ttctgataaa aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat
     6481 gaaaaactat tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa
     6541 tttaacgaaa gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg
     6601 ccaattggtt gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat
     6661 gaatactaaa tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa
     6721 atctaaatta gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa
     6781 caattaccat catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa
     6841 gaaatatcca aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg
     6901 taaaatgatt gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta
     6961 ctctaatatc atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa
     7021 acgccctcta atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga
     7081 ttttgccaca gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga
     7141 agtacagaca ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct
     7201 tattgctcgt aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt
     7261 agcttattca gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc
     7321 cgttaaagag ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat
     7381 tgacttttta gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc
     7441 taaatatagt ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga
     7501 attacaaaaa ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc
     7561 tagtcattat gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt
     7621 ggagcagcat aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg
     7681 tgttatttta gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga
     7741 caaaccaata cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg
     7801 agctcccgct gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac
     7861 aaaagaagtt ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg
     7921 cattgatttg agtcagctag gaggtgactg atggctggtt ggcgtactgt tgtggtaaat
     7981 accaagcttg atatcttgac agctagctca gtcctaggta taatactagt cgagaccatt
     8041 ggtctcagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
     8101 aaaaagtggc accgagtcgg tgcttttttt gatatcgaat tcctgcagcc cgggggatcc
     8161 actagttcta gagcggccgc caccgcggtg gagctcatcc caatggcgcg ccgagcttgg
     8221 ctcgagtgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct taactcaagc
     8281 gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag tctgctttta
     8341 ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca tgaaaggctg
     8401 gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat taaaatctag
     8461 cgagggcttt actaagctga tccggtggat gaccttttga atgaccttta atagattata
     8521 ttactaatta attggggacc ctagaggtcc ccttttttat tttaaaaatt ttttcacaaa
     8581 acggtttaca agcataaagc ttgctcaatc tgaaagcgac caggtgctcg gcgtggcaag
     8641 actcgcagcg aacccgtaga aagccatgct ccagccgccc gcattggaga aattcttcaa
     8701 attcccgttg cacatagccc ggcaattcct ttccctgctc tgccataagc gcagcgaatg
     8761 ccgggtaata ctcgtcaacg atctgataga gaagggtttg ctcgggtcgg tggctctggt
     8821 aacgaccagt atcccgatcc cggctggccg tcctggccgc cacatgaggc atgttccgcg
     8881 tccttgcaat actgtgttta catacagtct atcgcttagc ggaaagttct tttaccctca
     8941 gccgaaatgc ctgccgttgc tagacattgc cagccagtgc ccgtcactcc cgtactaact
     9001 gtcacgaacc cctgcaataa ctgtcacgcc cccctgcaat aactgtcacg aacccctgca
     9061 ataactgtca cgcccccaaa cctgcaaacc cagcaggggc gggggctggc ggggtgttgg
     9121 aaaaatccat ccatgattat ctaagaataa tccactaggc gcggttatca gcgcccttgt
     9181 ggggcgctgc tgcccttgcc caatatgccc ggccagaggc cggatagctg gtctattcgc
     9241 tgcgctaggc tacacaccgc cccaccgctg cgcggcaggg ggaaaggcgg gcaaagcccg
     9301 ctaaacccca caccaaaccc cgcagaaata cgctggagcg cttttagccg ctttagcggc
     9361 ctttccccct acccgaaggg tgggggcgcg tgtgcagccc cgcagggcct gtctcggtcg
     9421 atcattcagc ccggctcatc cttctggcgt ggcggcagac cgaacaaggc gcggtcgtgg
     9481 tcgcgttcaa ggtacgcatc cattgccgcc atgagccgat cctccggcca ctcgctgctg
     9541 ttcaccttgg ccaaaatcat ggcccccacc agcaccttgc gccttgtttc gttcttgcgc
     9601 tcttgctgct gttcccttgc ccgcacccgc tgaatttcgg cattgattcg cgctcgttgt
     9661 tcttcgagct tggccagccg atccgccgcc ttgttgctcc ccttaaccat cttgacaccc
     9721 cattgttaat gtgctgtctc gtaggctatc atggaggcac agcggcggca atcccgaccc
     9781 tactttgtag gggagggcgc acttaccggt ttctcttcga gaaactggcc taacggccac
     9841 ccttcgggcg gtgcgctctc cgagggccat tgcatggagc cgaaaagcaa aagcaacagc
     9901 gaggcagcat ggcgatttat caccttacgg cgaaaaccgg cagcaggtcg ggcggccaat
     9961 cggccagggc caaggccgac tacatccagc gcgaaggcaa gtatgcccgc gacatggatg
    10021 aagtcttgca cgccgaatcc gggcacatgc cggagttcgt cgagcggccc gccgactact
    10081 gggatgctgc cgacctgtat gaacgcgcca atgggcggct gttcaaggag gtcgaatttg
    10141 ccctgccggt cgagctgacc ctcgaccagc agaaggcgct ggcgtccgag ttcgcccagc
    10201 acctgaccgg tgccgagcgc ctgccgtata cgctggccat ccatgccggt ggcggcgaga
    10261 acccgcactg ccacctgatg atctccgagc ggatcaatga cggcatcgag cggcccgccg
    10321 ctcagtggtt caagcggtac aacggcaaga ccccggagaa gggcggggca cagaagaccg
    10381 aagcgctcaa gcccaaggca tggcttgagc agacccgcga ggcatgggcc gaccatgcca
    10441 accgggcatt agagcgggct ggccacgacg cccgcattga ccacagaaca cttgaggcgc
    10501 agggcatcga gcgcctgccc ggtgttcacc tggggccgaa cgtggtggag atggaaggcc
    10561 ggggcatccg caccgaccgg gcagacgtgg ccctgaacat cgacaccgcc aacgcccaga
    10621 tcatcgactt acaggaatac cgggaggcaa tagaccatga acgcaatcga cagagtgaag
    10681 aaatccagag gcatcaacga gttagcggag cagatcgaac cgctggccca gagcatggcg
    10741 acactggccg acgaagcccg gcaggtcatg agccagaccc agcaggccag cgaggcgcag
    10801 gcggcggagt ggctgaaagc ccagcgccag acaggggcgg catgggtgga gctggccaaa
    10861 gagttgcggg aggtagccgc cgaggtgagc agcgccgcgc agagcgcccg gagcgcgtcg
    10921 cgggggtggc actggaagct atggctaacc gtgatgctgg cttccatgat gcctacggtg
    10981 gtgctgctga tcgcatcgtt gctcttgctc gacctgacgc cactgacaac cgaggacggc
    11041 tcgatctggc tgcgcttggt ggcccgatga agaacgacag gactttgcag gccataggcc
    11101 gacagctcaa ggccatgggc tgtgagcgct tcgatatcgg cgtcagggac gccaccaccg
    11161 gccagatgat gaaccgggaa tggtcagccg ccgaagtgct ccagaacacg ccatggctca
    11221 agcggatgaa tgcccagggc aatgacgtgt atatcaggcc cgccgagcag gagcggcatg
    11281 gtctggtgct ggtggacgac ctcagcgagt ttgacttgga tgacatgaaa gccgagggcc
    11341 gggagcctgc cctggtagtg gaaaccagcc cgaagaacta tcaggcatgg gtcaaggtgg
    11401 ccgacgccgc aggcggtgaa cttcgggggc agattgcccg gacgctggcc agcgagtacg
    11461 acgccgaccc ggccagcgcc gacagccgcc actatggccg cttggcgggc ttcaccaacc
    11521 gcaaggacaa gcacaccacc cgcgccggtt atcagccgtg ggtgctgctg cgtgaatcca
    11581 agggcaagac cgccaccgct ggcccggcgc tggtgcagca ggctggccag cagatcgagc
    11641 aggcccagcg gcagcaggag aaggcccgca ggctggccag cctcgaactg cccgagcggc
    11701 agcttagccg ccaccggcgc acggcgctgg acgagtaccg cagcgagatg gccgggctgg
    11761 tcaagcgctt cggtgatgac ctcagcaagt gcgactttat cgccgcgcag aagctggcca
    11821 gccggggccg cagtgccgag gaaatcggca aggccatggc cgaggccagc ccagcgctgg
    11881 cagagcgcaa gcccggccac gaagcggatt acatcgagcg caccgtcagc aaggtcatgg
    11941 gtctgcccag cgtccagctt gcgcgggccg agctggcacg ggcaccggca ccccgccagc
    12001 gaggcatgga caggggcggg ccagatttca gcatgtagtg cttgcgttgg tactcacgcc
    12061 tgttatacta tgagtactca cgcacagaag ggggttttat ggaatacgaa aaaagcgctt
    12121 cagggtcggt ctacctgatc aaaagtgaca agggctattg gttgcccggt ggctttggtt
    12181 atacgtcaaa caaggccgag gctggccgct tttcagtcgc tgatatggcc agccttaacc
    12241 ttgacggctg caccttgtcc ttgttccgcg aagacaagcc tttcggcccc ggcaagtttc
    12301 tcggtgactg atatgaaaga ccaaaaggac aagcagaccg gcgacctgct ggccagccct
    12361 gacgctgtac gccaagcgcg atatgccgag cgcatgaagg ccaaagggat gcgtcagcgc
    12421 aagttctggc tgaccgacga cgaatacgag gcgctgcgcg agtgcctgga agaactcaga
    12481 gcggcgcagg gcgggggtag tgaccccgcc agcgcctaac caccaactgc ctgcaaagga
    12541 ggcaatcaat ggctacccat aagcctatca atattctgga ggcgttcgca gcagcgccgc
    12601 caccgctgga ctacgttttg cccaacatgg tggccggtac ggtcggggcg ctggtgtcgc
    12661 ccggtggtgc cggtaaatcc atgctggccc tgcaactggc cgcacagatt gcaggcgggc
    12721 cggatctgct ggaggtgggc gaactgccca ccggcccggt gatctacctg cccgccgaag
    12781 acccgcccac cgccattcat caccgcctgc acgcccttgg ggcgcacctc agcgccgagg
    12841 aacggcaagc cgtggctgac ggcctgctga tccagccgct gatcggcagc ctgcccaaca
    12901 tcatggcccc ggagtggttc gacggcctca agcgcgccgc cgagggccgc cgcctgatgg
    12961 tgctggacac gctgcgccgg ttccacatcg aggaagaaaa cgccagcggc cccatggccc
    13021 aggtcatcgg tcgcatggag gccatcgccg ccgataccgg gtgctctatc gtgttcctgc
    13081 accatgccag caagggcgcg gccatgatgg gcgcaggcga ccagcagcag gccagccggg
    13141 gcagctcggt actggtcgat aacatccgct ggcagtccta cctgtcgagc atgaccagcg
    13201 ccgaggccga ggaatggggt gtggacgacg accagcgccg gttcttcgtc cgcttcggtg
    13261 tgagcaaggc caactatggc gcaccgttcg ctgatcggtg gttcaggcgg catgacggcg
    13321 gggtgctcaa gcccgccgtg ctggagaggc agcgcaagag caagggggtg ccccgtggtg
    13381 aagcctaaga acaagcacag cctcagccac gtccggcacg acccggcgca ctgtctggcc
    13441 cccggcctgt tccgtgccct caagcggggc gagcgcaagc gcagcaagct ggacgtgacg
    13501 tatgactacg gcgacggcaa gcggatcgag ttcagcggcc cggagccgct gggcgctgat
    13561 gatctgcgca tcctgcaagg gctggtggcc atggctgggc ctaatggcct agtgcttggc
    13621 ccggaaccca agaccgaagg cggacggcag ctccggctgt tcctggaacc caagtgggag
    13681 gccgtcaccg ctgatgccat ggtggtcaaa ggtagctatc gggcgctggc aaaggaaatc
    13741 ggggcagagg tcgatagtgg tggggcgctc aagcacatac aggactgcat cgagcgcctt
    13801 tggaaggtat ccatcatcgc ccagaatggc cgcaagcggc aggggtttcg gctgctgtcg
    13861 gagtacgcca gcgacgaggc ggacgggcgc ctgtacgtgg ccctgaaccc cttgatcgcg
    13921 caggccgtca tgggtggcgg ccagcatgtg cgcatcagca tggacgaggt gcgggcgctg
    13981 gacagcgaaa ccgcccgcct gctgcaccag cggctgtgtg gctggatcga ccccggcaaa
    14041 accggcaagg cttccataga taccttgtgc ggctatgtct ggccgtcaga ggccagtggt
    14101 tcgaccatgc gcaagcgccg ccagcgggtg cgcgaggcgt tgccggagct ggtcgcgctg
    14161 ggctggacgg taaccgagtt cgcggcgggc aagtacgaca tcacccggcc caaggcggca
    14221 ggctgacccc ccccactcta ttgtaaacaa gacattttta tcttttatat tcaatggctt
    14281 attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg cttaacaata
    14341 ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt tcctggcttt
    14401 gcttccagat gtatgctctt ctgctctagc tagaggatcg atccttttta acccat
//