LOCUS       U6876GA280-2        1683 bp    DNA     circular     10-Sep-2024
DEFINITION  1_FUS_1-284_OPT_original seq_RP1B
SOURCE      
  ORGANISM  
COMMENT     http://www.genscript.com/
COMMENT     This file is created by GenScript
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|U6876GA280-2,1_FUS_1-284_OPT_original seq_RP1B|
COMMENT     VNTAUTHORNAME|GenScript|
FEATURES             Location/Qualifiers
     misc_feature    43..962
                     /note="Insert Sequence"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /label="6xHis"
                     /codon_start=1
                     /product="6xHis affinity tag"
     CDS             963..980
                     /label="6xHis"
                     /codon_start=1
                     /product="6xHis affinity tag"
     terminator      1047..1094
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     rep_origin      1131..1586
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
BASE COUNT     406 a      439 c      482 g      356 t 
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcgagc aacgactaca
      121 cccagcaagc gacccagagc tatggcgcgt acccgaccca gccgggccaa ggttacagcc
      181 agcaaagcag ccagccgtac ggtcaacaga gctacagcgg ttacagccaa agcaccgata
      241 ccagcggtta cggccaaagc agctatagca gctatggtca gagccaaaac accggttatg
      301 gtacccagag caccccgcag ggctatggta gcaccggcgg ttacggtagc agccagagca
      361 gccagagcag ctacggtcag caaagcagct acccgggcta cggtcaacag ccggcgccga
      421 gcagcaccag cggtagctat ggtagcagca gccaaagcag cagctatggc cagccgcaaa
      481 gcggtagcta cagccaacag ccgagctacg gtggccaaca gcaaagctac ggtcaacagc
      541 agagctataa cccgccgcag ggttacggtc agcaaaacca gtacaacagc agcagcggcg
      601 gcggtggtgg tggcggtggc ggcggtaact atggccagga tcaaagcagc atgagcagcg
      661 gtggtggtag cggtggcggc tacggtaacc aggaccaaag cggcggtggt ggcagcggcg
      721 gttatggtca gcaagatcgt ggtggccgtg gccgtggtgg cagcggtggt ggcggcggcg
      781 gtggcggtgg tggctacaac cgtagcagcg gcggttacga gccgcgtggt cgtggtggtg
      841 gccgtggtgg tcgtggcggt atgggcggta gcgatcgtgg cggtttcaac aagtttggtg
      901 gcccgcgtga tcagggtagc cgtcacgata gcgaacagga caacagcgat aactgactcg
      961 agcaccacca ccaccaccac tgagatccgg ctgctaacaa agcccgaaag gaagctgagt
     1021 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     1081 tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg acgcgccctg
     1141 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     1201 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     1261 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     1321 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     1381 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     1441 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     1501 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     1561 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     1621 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa ttaattctta
     1681 gaa
//


