LOCUS       U1977HH090-8        1320 bp    DNA     circular     10-Sep-2024
DEFINITION  FUS1_163_QQ4xGG_RP1B
SOURCE      
  ORGANISM  
COMMENT     http://www.genscript.com/
COMMENT     This file is created by GenScript
COMMENT     ORIGDB|GenBank
COMMENT     VNTNAME|U1977HH090-8,FUS1_163_QQ4xGG_RP1B|
COMMENT     VNTAUTHORNAME|GenScript|
FEATURES             Location/Qualifiers
     misc_feature    43..599
                     /note="Insert Sequence"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             63..80
                     /label="6xHis"
                     /codon_start=1
                     /product="6xHis affinity tag"
     CDS             600..617
                     /label="6xHis"
                     /codon_start=1
                     /product="6xHis affinity tag"
     terminator      684..731
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA polymerase"
     rep_origin      768..1223
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
BASE COUNT     319 a      357 c      313 g      331 t 
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata taccatgggc tctgataaaa
       61 ttcatcacca tcaccatcac gaaaatcttt attttcaagg tatggcttca aatgattata
      121 cacagcaagc aactcaatct tacggcgcgt atccgaccca gccgggtcag ggttacagcg
      181 gtggtagctc gcaaccgtat ggtcaacagt cctacagcgg ttattctcaa tctacggaca
      241 ccagcggcta tggtcaaagc agctatagct cgtacggcca gtcgcaaaac accggttatg
      301 gcacccagtc tactccgcag ggttatggca gcaccggtgg ctacggcagc tcccagtcct
      361 cccaatcctc ctacggcggt ggctcctctt acccgggtta cggccagcag ccagctccga
      421 gttcaacgag cggtagctac ggcagctcct cgcaaagcag cagctatggc cagccgcagt
      481 ctggctcgta cagcggtggt ccgagctacg gtggtcagca acaaagctac ggccaacaac
      541 agagctataa tccgcctcag ggttacggcg gtgggaacca gtataacagc tgactcgagc
      601 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      661 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      721 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      781 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      841 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      901 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      961 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1021 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1081 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1141 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1201 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     1261 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgaatta attcttagaa
//


