LOCUS       U0943HI160-1         920 bp DNA     linear   SYN 09-SEP-2024
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             21..38
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHH"
     CDS             39..59
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /note="color: #cc99b2
                     Cleavage site after base 56"
                     /translation="ENLYFQG"
     CDS             complement(727..750)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /note="color: #cc99b2"
                     /translation="HHHHHHHH"
ORIGIN
        1 ccatgggctc tgataaaatt catcaccatc accatcacga aaatctttat tttcaaggta
       61 tggctagtaa tgattatact tcatctgcaa caagctccta tggcgcgtat ccgaccagcc
      121 cgggtagcgg ttatagcagt tcgagctcct ctccttatgg ttcttcgagc tacagcggct
      181 acagctccag cacggatacc agcggttacg gctcctcgtc ttacagctcc tatggcagct
      241 cctctaacac cggttacggc accagctcta ctccgagcgg ttatggttct acgggtggct
      301 acggcagttc ctcttcttct tccagcagct acggctcctc atcgtcttac ccgggttacg
      361 gttccagccc ggctccgagc tcaaccagcg gcagctacgg tagctctagc agcagctcgt
      421 cgtacggctc cccgagctcg ggcagctata gcagttcccc gtcatacggc ggcagcagca
      481 gctcgtatgg cagcagctct tcgtacaacc cgccaagcgg ttatggctca tccaatagct
      541 ataacagctc ctccgggggt ggcggtggtg gcggtggcgg cgggaactat ggccaagacc
      601 agagcagcat gagcagcggc ggtggtagcg gtgggggcta cggcaatcag gaccaaagcg
      661 gtggcggtgg cagcggtggt tacggccagc aggaccgcgg tggccgtggt cgtggcggat
      721 ccggcggtgg tggtggtggt ggtggtggtg gctataatcg tagctccggt ggctacgagc
      781 cgcgtggtcg cggtggtggc agaggtggac gcggtggcat gggtggctcg gatcgtggtg
      841 gttttaacaa gttcggtggc ccacgtgatc aaggttctcg tcacgatagc gaacaggaca
      901 acagcgacaa ttgactcgag
//
