LOCUS       pCKC017__HF_RE_c       10267 bp ds-DNA     linear       06-SEP-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pCKC017 (HF-RE
            cloning, C to T in pPGK BamHI, Cas9 targ after TdT WPRE, RSs for
            library screening)
            pLenti-Kan-target-barcode-pCMV-TdTbaseofloopNNK-sfGFP-T2A-puro.gbk
            ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    27..131
                     /label="ampR cassette"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="ampR cassette"
                     /label="ampR cassette"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    132..926
                     /label="neor/kanr"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="neor/kanr"
                     /label="neor/kanr"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    2381..2561
                     /label="truncated 5' LTR"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="truncated 5' LTR"
                     /label="truncated 5' LTR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4091..4777
                     /label="pCMV"
                     /ApEinfo_revcolor="#1400ff"
                     /ApEinfo_fwdcolor="#1400ff"
                     /locus_tag="pCMV"
                     /label="pCMV"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4114..4119
                     /label="PstI site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="PstI site"
                     /label="PstI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4120..4159
                     /label="1st round F"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /locus_tag="1st round F"
                     /label="1st round F"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4778..4783
                     /label="NheI site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /locus_tag="NheI site"
                     /label="NheI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4784..4799
                     /label="pPGK1?"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="pPGK1?"
                     /label="pPGK1?"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4800..6329
                     /label="TdT"
                     /ApEinfo_revcolor="#a126ff"
                     /ApEinfo_fwdcolor="#a126ff"
                     /locus_tag="TdT"
                     /label="TdT"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4802..4807
                     /label="BamHI site removed"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="BamHI site removed"
                     /label="BamHI site removed"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5951..5971
                     /label="library_1-NGS-F"
                     /ApEinfo_revcolor="#0432ff"
                     /ApEinfo_fwdcolor="#0432ff"
                     /locus_tag="library_1-NGS-F"
                     /label="library_1-NGS-F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    5952..5972
                     /label="library_2-NGS-F"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="library_2-NGS-F"
                     /label="library_2-NGS-F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    5967..5990
                     /label="1st round R"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /locus_tag="1st round R"
                     /label="1st round R"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5982..5984
                     /label="NNKs for library 2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="NNKs for library 2"
                     /label="NNKs for library 2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5988..5993
                     /label="NNKs for library 2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="NNKs for library 2(1)"
                     /label="NNKs for library 2(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    5991..5999
                     /label="NNKs for library 1"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="NNKs for library 1"
                     /label="NNKs for library 1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6039..6044
                     /label="SalI site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="SalI site"
                     /label="SalI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6073..6093
                     /label="library_3-NGS-F"
                     /ApEinfo_revcolor="#011892"
                     /ApEinfo_fwdcolor="#011892"
                     /locus_tag="library_3-NGS-F"
                     /label="library_3-NGS-F"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6150..6155
                     /label="BamHI site (for library screening)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="BamHI site (for library screening)"
                     /label="BamHI site (for library screening)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6156..6158
                     /label="NNK for library 3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="NNK for library 3(2)"
                     /label="NNK for library 3(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6165..6167
                     /label="NNK for library 3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="NNK for library 3(1)"
                     /label="NNK for library 3(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6177..6179
                     /label="NNK for library 3"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="NNK for library 3"
                     /label="NNK for library 3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6924..6929
                     /label="BamHI RS (for library screening)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="BamHI RS (for library screening)"
                     /label="BamHI RS (for library screening)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6930..6949
                     /label="misc_feature"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="misc_feature"
                     /label="misc_feature"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6950..6952
                     /label="misc_feature"
                     /ApEinfo_revcolor="#0080c0"
                     /ApEinfo_fwdcolor="#0080c0"
                     /locus_tag="misc_feature(1)"
                     /label="misc_feature(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6953..6966
                     /label="site3 PCR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="site3 PCR"
                     /label="site3 PCR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6953..6977
                     /label="library_2-NGS-R"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="library_2-NGS-R"
                     /label="library_2-NGS-R"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6953..6978
                     /label="library_1-NGS-R"
                     /ApEinfo_revcolor="#0432ff"
                     /ApEinfo_fwdcolor="#0432ff"
                     /locus_tag="library_1-NGS-R"
                     /label="library_1-NGS-R"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6967..6972
                     /label="BamHI site out (GGATCC -> GGATCt)"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="BamHI site out (GGATCC -> GGATCt)"
                     /label="BamHI site out (GGATCC -> GGATCt)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6967..7543
                     /label="pPGK1"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="pPGK1"
                     /label="pPGK1"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    7044..7065
                     /label="library_3-NGS-R"
                     /ApEinfo_revcolor="#011892"
                     /ApEinfo_fwdcolor="#011892"
                     /locus_tag="library_3-NGS-R"
                     /label="library_3-NGS-R"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    7522..7527
                     /label="mutated NheI site"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /locus_tag="mutated NheI site"
                     /label="mutated NheI site"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    7528..7543
                     /label="Kozak"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="Kozak"
                     /label="Kozak"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    7544..8257
                     /label="sfGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="sfGFP"
                     /label="sfGFP"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    8258..8317
                     /label="P2A"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
                     /locus_tag="P2A"
                     /label="P2A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    8318..8914
                     /label="puroR"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="puroR"
                     /label="puroR"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    8426..8431
                     /label="SalI cloned out"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="SalI cloned out"
                     /label="SalI cloned out"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    9004..9237
                     /label="3' LTR delta U3"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="3' LTR delta U3"
                     /label="3' LTR delta U3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
ORIGIN
        1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
       61 attcaaatAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA
      121 AAAGGAAGAG TATGATTGAA CAAGATGGAT TGCACGCTGG TTCTCCGGCC GCTTGGGTGG
      181 AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT GCCGCCGTGT
      241 TCCGTCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG TCCGGTGCCC
      301 TGAATGAACT GCAAGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG GGCGTTCCTT
      361 GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG
      421 TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA TCCATCATGG
      481 CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC TTGCCCATTC GACCACCAAG
      541 CGAAACATCg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
      601 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
      661 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
      721 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
      781 gctatcagga catagcgttg gctacccgtg atattgctga agAGCTTGGC GGCGAATGGG
      841 CTGACCGCTT CCTCGTGCTT TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT
      901 ATCGCCTTCT TGACGAGTTC TTCTGACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA
      961 TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC
     1021 TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA
     1081 AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA
     1141 AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC
     1201 CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTTCTTCTA GTGTAGCCGT
     1261 AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC
     1321 TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC
     1381 GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA
     1441 GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG
     1501 CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG
     1561 GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT
     1621 TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT
     1681 GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC
     1741 ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT
     1801 GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG
     1861 CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA
     1921 GCTGGCACGA CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA
     1981 GTTAGCTCAC TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT
     2041 GTGGAATTGT GAGCGGATAA CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGCCA
     2101 AGCGCGCAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA GCTGCAAGCT TAATGTAGTC
     2161 TTATGCAATA CTCTTGTAGT CTTGCAACAT GGTAACGATG AGTTAGCAAC ATGCCTTACA
     2221 AGGAGAGAAA AAGCACCGTG CATGCCGATT GGTGGAAGTA AGGTGGTACG ATCGTGCCTT
     2281 ATTAGGAAGG CAACAGACGG GTCTGACATG GATTGGACGA ACCACTGAAT TGCCGCATTG
     2341 CAGAGATATT GTATTTAAGT GCCTAGCTCG ATACATAAAC GGGTCTCTCT GGTTAGACCA
     2401 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG
     2461 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG
     2521 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC
     2581 TTGAAAGCGA AAGGGAAACC AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG
     2641 CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA
     2701 GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG
     2761 CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA
     2821 GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA
     2881 GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA
     2941 CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA
     3001 AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC
     3061 GCACAGCAAG CGGCCGCTGA TCTTCAGACC TGGagGAGGA GATATGAGGG ACAATTGGAG
     3121 AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA
     3181 GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT
     3241 TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA
     3301 GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA
     3361 GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT
     3421 CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG
     3481 AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA
     3541 ACAGATTTGG AATCACACGA CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG
     3601 CTTAATACAC TCCTTAATTG AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT
     3661 ATTGGAATTA GATAAATGGG CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG
     3721 GTATATAAAA TTATTCATAA TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC
     3781 TGTACTTTCT ATAGTGAATA GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA
     3841 CCTCCCAACC CCGAGGGGAC CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG
     3901 AGACAGAGAC AGATCCATTC GATTAGTGAA CGGATCTCGA CGGTATCGGT TAACTTTTAA
     3961 AAGAAAAGGG GGGATTGGGG GGTACAGTGC AGGGGAAAGA ATAGTAGACA TAATAGCAAC
     4021 AGACATACAA ACTAAAGAAT TACAAAAACA AATTACAAAA TTCAAAATTT TATCGATAAG
     4081 CTTGGGAGTT CCGCGTTACA TAACTTACGG TAActgcagC ATTGATTATT GACTAGTTAT
     4141 TAATAGTAAT CAATTACGGG GTCATTAGTT CATAGCCCAT ATATGGAGTT CCGCGTTACA
     4201 TAACTTACGG TAAATGGCCC GCCTGGCTGA CCGCCCAACG ACCCCCGCCC ATTGACGTCA
     4261 ATAATGACGT ATGTTCCCAT AGTAACGCCA ATAGGGACTT TCCATTGACG TCAATGGGTG
     4321 GAGTATTTAC GGTAAACTGC CCACTTGGCA GTACATCAAG TGTATCATAT GCCAAGTACG
     4381 CCCCCTATTG ACGTCAATGA CGGTAAATGG CCCGCCTGGC ATTATGCCCA GTACATGACC
     4441 TTATGGGACT TTCCTACTTG GCAGTACATC TACGTATTAG TCATCGCTAT TACCATGGTG
     4501 ATGCGGTTTT GGCAGTACAT CAATGGGCGT GGATAGCGGT TTGACTCACG GGGATTTCCA
     4561 AGTCTCCACC CCATTGACGT CAATGGGAGT TTGTTTTGGC ACCAAAATCA ACGGGACTTT
     4621 CCAAAATGTC GTAACAACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG
     4681 GAGGTCTATA TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC
     4741 GAAATTAATA CGACTCACTA TAGGGAGACC CAAGCTGGCT AGCGCTACCG GTCGCCACCA
     4801 TGGAcCCACC ACGAGCGTCC CACTTGAGCC CTCGGAAGAA GAGACCCCGG CAGACGGGTG
     4861 CCTTGATGGC CTCCTCTCCT CAAGACATCA AATTTCAAGA TTTGGTCGTC TTCATTTTGG
     4921 AGAAGAAAAT GGGAACCACC CGCAGAGCGT TCCTCATGGA GCTGGCCCGC AGGAAAGGGT
     4981 TCAGGGTTGA AAATGAGCTC AGTGATTCTG TCACCCACAT TGTAGCAGAG AACAACTCGG
     5041 GTTCGGATGT TCTGGAGTGG CTTCAAGCAC AGAAAGTACA AGTCAGCTCA CAACCAGAGC
     5101 TCCTCGATGT CTCCTGGCTG ATCGAATGCA TAGGAGCAGG GAAACCGGTG GAAATGACAG
     5161 GAAAACACCA GCTTGTTGTG AGAAGAGACT ATTCAGATAG CACCAACCCA GGCCCCCCGA
     5221 AGACTCCACC AATTGCTGTA CAAAAGATCT CCCAGTATGC GTGTCAGAGA AGAACCACTT
     5281 TAAACAACTG TAACCAGATA TTCACGGATG CCTTTGATAT ACTGGCTGAA AACTGTGAGT
     5341 TTAGAGAAAA TGAAGACTCC TGTGTGACAT TTATGAGAGC AGCTTCTGTA TTGAAATCTC
     5401 TGCCATTCAC AATCATCAGT ATGAAGGACA CAGAAGGAAT TCCCTGCCTG GGGTCCAAGG
     5461 TGAAGGGTAT CATAGAGGAG ATTATTGAAG ATGGAGAAAG TTCTGAAGTT AAAGCTGTGT
     5521 TAAATGATGA ACGATATCAA TCCTTCAAAC TCTTTACTTC TGTATTTGGA GTGGGGCTGA
     5581 AGACTTCTGA GAAGTGGTTC AGGATGGGTT TCAGAACTCT GAGTAAAGTA AGGTCGGACA
     5641 AAAGCCTGAA ATTTACACGA ATGCAGAAAG CAGGATTTCT GTATTATGAA GACCTTGTCA
     5701 GCTGTGTGAC CAGGGCAGAA GCAGAGGCCG TCAGTGTGCT GGTTAAAGAG GCTGTCTGGG
     5761 CATTTCTTCC GGATGCTTTC GTCACCATGA CAGGAGGGTT CCGGAGGGGT AAGAAGATGG
     5821 GGCATGATGT AGATTTTTTA ATTACCAGCC CAGGATCAAC AGAGGATGAA GAGCAACTTT
     5881 TACAGAAAGT GATGAACTTA TGGGAAAAGA AGGGATTACT TTTATATTAT GACCTTGTGG
     5941 AGTCAACATT TGAAAAGCTC AGGTTGCCTA GCAGGAAGGT TGATGCTTTG GATCATTTTC
     6001 AAAAGTGCTT TCTGATTTTC AAATTGCCTC GTCAAAGAGT cGACAGTGAC CAGTCCAGCT
     6061 GGCAGGAAGG AAAGACCTGG AAGGCCATCC GTGTGGATTT AGTTCTGTGC CCCTACGAGC
     6121 GTCGTGCCTT TGCCCTGTTG GGATGGACTG GaTCCCGGCA GTTTGAGAGA GACCTCCGGC
     6181 GCTATGCCAC ACATGAGCGG AAGATGATTC TGGATAACCA TGCTTTATAT GACAAGACCA
     6241 AGAGGATATT CCTCAAAGCA GAAAGTGAAG AAGAAATTTT TGCGCATCTG GGATTGGATT
     6301 ATATTGAACC GTGGGAAAGA AATGCCTAGC AATCAACCTC TGGATTACAA AATTTGTGAA
     6361 AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA CGCTGCTTTA
     6421 ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC CTTGTATAAA
     6481 TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG TGGCGTGGTG
     6541 TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC CTGTCAGCTC
     6601 CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT CGCCGCCTGC
     6661 CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT GGTGTTGTCG
     6721 GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT TCTGCGCGGG
     6781 ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC CCGCGGCCTG
     6841 CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC CTCAGACGAG TCGGATCTCC
     6901 CTTTGGGCCG CCTCCCCGCC TGGggatccG GCCCAGACTG AGCACGTGAG ggcagaggaa
     6961 aggaagGGAT CtATTAGGCG GCCGCGTGGA TAACCGTATT ACCGCCATGC ATCTACCGGG
     7021 TAGGGGAGGC GCTTTTCCCA AGGCAGTCTG GAGCATGCGC TTTAGCAGCC CCGCTGGGCA
     7081 CTTGGCGCTA CACAAGTGGC CTCTGGCCTC GCACACATTC CACATCCACC GGTAGGCGCC
     7141 AACCGGCTCC GTTCTTTGGT GGCCCCTTCG CGCCACCTTC TACTCCTCCC CTAGTCAGGA
     7201 AGTTCCCCCC CGCCCCGCAG CTCGCGTCGT GCAGGACGTG ACAAATGGAA GTAGCACGTA
     7261 TCACTAGTCT CGTGCAGATG GACAGCACCG CTGAGCAATG GAAGCGGGTA GGCCTTTGGG
     7321 GCAGCGGCCA ATAGCAGCTT TGCTCCTTCG CTTTCTGGGC TCAGAGGCTG GGAAGGGGTG
     7381 GGTCCGGGGG CGGGCTCAGG GGCGGGCTCA GGGGCGGGGC GGGCGCCCGA AGGTCCTCCG
     7441 GAGGCCCGGC ATTCTGCACG CTTCAAAAGC GCACGTCTGC CGCGCTGTTC TCCTCTTCCT
     7501 CATCTCCGGG CCTTTCGACC TGGTAGCgct accggtcgcc accatgcgta aagGCGAAGA
     7561 GCTGTTCACT GGTGTCGTCC CTATTCTGGT GGAACTGGAT GGTGATGTCA ACGGTCATAA
     7621 GTTTTCCGTG CGTGGCGAGG GTGAAGGTGA CGCAACTAAT GGTAAACTGA CGCTGAAGTT
     7681 CATCTGTACT ACTGGTAAAC TGCCGGTTCC TTGGCCGACT CTGGTAACGA CGCTGACTTA
     7741 TGGTGTTCAG TGCTTTGCTC GTTATCCGGA CCATATGAAG CAGCATGACT TCTTCAAGTC
     7801 CGCCATGCCG GAAGGCTATG TGCAGGAACG CACGATTTCC TTTAAGGATG ACGGCACGTA
     7861 CAAAACGCGT GCGGAAGTGA AATTTGAAGG CGATACCCTG GTAAACCGCA TTGAGCTGAA
     7921 AGGCATTGAC TTTAAAGAGG ACGGCAATAT CCTGGGCCAT AAGCTGGAAT ACAATTTTAA
     7981 CAGCCACAAT GTTTACATCA CCGCCGATAA ACAAAAAAAT GGCATTAAAG CGAATTTTAA
     8041 AATTCGCCAC AACGTGGAGG ATGGCAGCGT GCAGCTGGCT GATCACTACC AGCAAAACAC
     8101 TCCAATCGGT GATGGTCCTG TTCTGCTGCC AGACAATCAC TATCTGAGCA CGCAAAGCGT
     8161 TCTGTCTAAA GATCCGAACG AGAAACGCGA TCATATGGTT CTGCTGGAGT TCGTAACCGC
     8221 AGCGGGCATC ACGCATGGTA TGGATGAACT GTACAAAGGC GCCACCAACT TCTCCCTGCT
     8281 GAAGCAGGCC GGCGACGTGG AGGAGAACCC CGGCCCCATG ACCGAGTACA AGCCCACGGT
     8341 GCGCCTCGCC ACCCGCGACG ACGTCCCCCG GGCCGTACGC ACCCTCGCCG CCGCGTTCGC
     8401 CGACTACCCC GCCACGCGCC ACACCGTgGA CCCGGACCGC CACATCGAGC GGGTCACCGA
     8461 GCTGCAAGAA CTCTTCCTCA CGCGCGTCGG GCTCGACATC GGCAAGGTGT GGGTCGCGGA
     8521 CGACGGCGCC GCGGTGGCGG TCTGGACCAC GCCGGAGAGC GTCGAAGCGG GGGCGGTGTT
     8581 CGCCGAGATC GGCCCGCGCA TGGCCGAGTT GAGCGGTTCC CGGCTGGCCG CGCAGCAACA
     8641 GATGGAAGGC CTCCTGGCGC CGCACCGGCC CAAGGAGCCC GCGTGGTTCC TGGCCACCGT
     8701 CGGAGTCTCG CCCGACCACC AGGGCAAGGG TCTGGGCAGC GCCGTCGTGC TCCCCGGAGT
     8761 GGAGGCGGCC GAGCGCGCCG GGGTGCCCGC CTTCCTGGAG ACCTCCGCGC CCCGCAACCT
     8821 CCCCTTCTAC GAGCGGCTCG GCTTCACCGT CACCGCCGAC GTCGAGGTGC CCGAAGGACC
     8881 GCGCACCTGG TGCATGACCC GCAAGCCCGG TGCCTGAGCA CAATTCGAGC TCGGTACCTT
     8941 TAAGACCAAT GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG
     9001 GACTGGAAGG GCTAGCTCAC TCCCAACGAA GACAAGATCT GCTTTTTGCT TGTACTGGGT
     9061 CTCTCTGGTT AGACCAGATC TGAGCCTGGG AGCTCTCTGG CTAACTAGGG AACCCACTGC
     9121 TTAAGCCTCA ATAAAGCTTG CCTTGAGTGC TTCAAGTAGT GTGTGCCCGT CTGTTGTGTG
     9181 ACTCTGGTAA CTAGAGATCC CTCAGACCCT TTTAGTCAGT GTGGAAAATC TCTAGCAGTA
     9241 GTAGTTCATG TCATCTTATT ATTCAGTATT TATAACTTGC AAAGAAATGA ATATCAGAGA
     9301 GTGAGAGGAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA
     9361 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA
     9421 ATGTATCTTA TCATGTCTGG CTCTAGCTAT CCCGCCCCTA ACTCCGCCCA TCCCGCCCCT
     9481 AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT TTATTTATGC
     9541 AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG GCTTTTTTGG
     9601 AGGCCTAGGG ACGTACCCAA TTCGCCCTAT AGTGAGTCGT ATTACGCGCG CTCACTGGCC
     9661 GTCGTTTTAC AACGTCGTGA CTGGGAAAAC CCTGGCGTTA CCCAACTTAA TCGCCTTGCA
     9721 GCACATCCCC CTTTCGCCAG CTGGCGTAAT AGCGAAGAGG CCCGCACCGA TCGCCCTTCC
     9781 CAACAGTTGC GCAGCCTGAA TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG
     9841 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT
     9901 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA
     9961 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA
    10021 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT
    10081 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC
    10141 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG
    10201 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT
    10261 ACAATTT
//