LOCUS       pCKC776__confirm       10834 bp ds-DNA     circular     06-SEP-2024
DEFINITION  .
COMMENT     Imported using the Genbank importer. File name: pCKC776 (confirmed)
            MTK0-PB-4xHRE-YB_TATA-hCas9-ODD-T2A-TdT(G:C-DQFDRDLR +
            DAMD)-mCherry-noBsaI-noextraEsp3I copy.gb ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label="UNSa02"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /locus_tag="UNSa02"
                     /label="UNSa02"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_structure  53..107
                     /label="HRE v2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="HRE v2"
                     /locus_tag="HRE v2"
                     /label="HRE v2"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    72..102
                     /label="HREv2 consensus"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="HREv2 consensus"
                     /locus_tag="HREv2 consensus(1)"
                     /label="HREv2 consensus(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_structure  108..146
                     /label="HRE v2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="HRE v2"
                     /locus_tag="HRE v2(1)"
                     /label="HRE v2(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    111..141
                     /label="HREv2 consensus"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="HREv2 consensus"
                     /locus_tag="HREv2 consensus(2)"
                     /label="HREv2 consensus(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_structure  147..185
                     /label="HRE v2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="HRE v2"
                     /locus_tag="HRE v2(2)"
                     /label="HRE v2(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    150..180
                     /label="HREv2 consensus"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="HREv2 consensus"
                     /locus_tag="HREv2 consensus"
                     /label="HREv2 consensus"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_structure  186..224
                     /label="HRE v2"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="HRE v2"
                     /locus_tag="HRE v2(3)"
                     /label="HRE v2(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    189..219
                     /label="HREv2 consensus"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="HREv2 consensus"
                     /locus_tag="HREv2 consensus(3)"
                     /label="HREv2 consensus(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    237..261
                     /label="Minimal Promoter from Benenson Lab"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="Minimal Promoter from Benenson Lab"
                     /locus_tag="Minimal Promoter from Benenson Lab"
                     /label="Minimal Promoter from Benenson Lab"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    246..252
                     /label="TATA Box"
                     /ApEinfo_revcolor="#a700ff"
                     /ApEinfo_fwdcolor="#a700ff"
                     /note="TATA Box"
                     /locus_tag="TATA Box"
                     /label="TATA Box"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    309..326
                     /label="hCas9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="hCas9(1)"
                     /label="hCas9(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    330..4463
                     /label="hCas9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="hCas9"
                     /label="hCas9"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4443..4463
                     /label="SV40 NLS (keep with Cas9)"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="SV40 NLS (keep with Cas9)"
                     /label="SV40 NLS (keep with Cas9)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    4464..4478
                     /label="Linker"
                     /ApEinfo_revcolor="#d840ff"
                     /ApEinfo_fwdcolor="#d840ff"
                     /locus_tag="Linker"
                     /label="Linker"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4479..4847
                     /label="ODD"
                     /ApEinfo_revcolor="#4110ff"
                     /ApEinfo_fwdcolor="#4110ff"
                     /locus_tag="ODD"
                     /label="ODD"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4848..4859
                     /label="Linker"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="Linker(1)"
                     /label="Linker(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4848..4880
                     /label="BE3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="BE3"
                     /label="BE3"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4848..4880
                     /label="Putative linker?"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="Putative linker?"
                     /label="Putative linker?"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4860..4880
                     /label="Classical NLS"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /locus_tag="Classical NLS"
                     /label="Classical NLS"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    complement(4881..4943)
                     /label="T2A"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="T2A"
                     /label="T2A"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    4944..6125
                     /label="TdT"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
                     /locus_tag="TdT(1)"
                     /label="TdT(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6126..6137
                     /label="DAMD"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="DAMD"
                     /label="DAMD"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6126..6137
                     /label="TdT"
                     /ApEinfo_revcolor="#a126ff"
                     /ApEinfo_fwdcolor="#a126ff"
                     /locus_tag="TdT"
                     /label="TdT"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6138..6299
                     /label="TdT"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
                     /locus_tag="TdT(3)"
                     /label="TdT(3)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6296..6296
                     /label="SNP from cloning (synonymous)"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /locus_tag="SNP from cloning (synonymous)"
                     /label="SNP from cloning (synonymous)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6300..6323
                     /label="TdT"
                     /ApEinfo_revcolor="#a126ff"
                     /ApEinfo_fwdcolor="#a126ff"
                     /locus_tag="TdT(4)"
                     /label="TdT(4)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6300..6323
                     /label="DQFDRDLR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="DQFDRDLR"
                     /label="DQFDRDLR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6300..6323
                     /label="cloning - 2nd round R"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="cloning - 2nd round R"
                     /label="cloning - 2nd round R"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6324..6473
                     /label="TdT"
                     /ApEinfo_revcolor="#8000ff"
                     /ApEinfo_fwdcolor="#8000ff"
                     /locus_tag="TdT(2)"
                     /label="TdT(2)"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    6572..6619
                     /label="HSV TK pA"
                     /ApEinfo_revcolor="#ff40ff"
                     /ApEinfo_fwdcolor="#ff40ff"
                     /locus_tag="HSV TK pA"
                     /label="HSV TK pA"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     protein_bind    6650..6653
                     /label="BsmBI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6650..6674
                     /label="ConE scar"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /locus_tag="ConE scar"
                     /label="ConE scar"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6675..6857
                     /label="Insulator A1"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /locus_tag="Insulator A1"
                     /label="Insulator A1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    6890..7347
                     /label="truncated pEF1a"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="truncated pEF1a"
                     /label="truncated pEF1a"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}width 5 offset 0"
     misc_feature    7348..7350
                     /label="BsaI"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    7351..8069
                     /label="mCherry"
                     /ApEinfo_revcolor="#ff3129"
                     /ApEinfo_fwdcolor="#ff3129"
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     Terminator      8083..8316
                     /label="BGH-PA"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /locus_tag="BGH-PA"
                     /label="BGH-PA"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        8333..8580
                     /label="3' PB repeat"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="3' PB repeat"
                     /label="3' PB repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    complement(8360..8365)
                     /label="CAAAAT repeat"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /locus_tag="CAAAAT repeat"
                     /label="CAAAAT repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    8508..8526
                     /label="19bp IR"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /locus_tag="19bp IR(1)"
                     /label="19bp IR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    8527..8557
                     /label="31bp spacer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /locus_tag="31bp spacer"
                     /label="31bp spacer"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    8558..8570
                     /label="13bp TR"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
                     /locus_tag="13bp TR"
                     /label="13bp TR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer_bind     complement(8620..8639)
                     /label="T3 (Elim)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="T3 (Elim)"
                     /label="T3 (Elim)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        complement(8621..8639)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     terminator      complement(8652..8678)
                     /label="KanR Terminator"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="KanR Terminator"
                     /label="KanR Terminator"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     CDS             complement(8679..9494)
                     /label="KanR"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     misc_feature    9021..9021
                     /label="SNP from cloning (doesn't affect kanR)"
                     /ApEinfo_revcolor="#ff2600"
                     /ApEinfo_fwdcolor="#ff2600"
                     /locus_tag="SNP from cloning (doesn't affect kanR)"
                     /label="SNP from cloning (doesn't affect kanR)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        complement(9495..9642)
                     /label="KanR Promoter"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /locus_tag="KanR Promoter"
                     /label="KanR Promoter"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     rep_origin      complement(9643..10406)
                     /label="ColE1"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     primer_bind     10419..10438
                     /label="T7 Forward (Elim)"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
                     /locus_tag="T7 Forward (Elim)"
                     /label="T7 Forward (Elim)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     promoter        10463..10784
                     /label="5' PB repeat"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /locus_tag="5' PB repeat"
                     /label="5' PB repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    10467..10479
                     /label="13bp TR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /locus_tag="13bp TR(1)"
                     /label="13bp TR(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    10483..10501
                     /label="19bp IR"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /locus_tag="19bp IR"
                     /label="19bp IR"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    complement(10624..10629)
                     /label="CAAAT repeat"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /locus_tag="CAAAT repeat(1)"
                     /label="CAAAT repeat(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    10651..10656
                     /label="CAAAT repeat"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /locus_tag="CAAAT repeat"
                     /label="CAAAT repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     misc_feature    10741..10747
                     /label="ACTTATT repeat"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
                     /locus_tag="ACTTATT repeat"
                     /label="ACTTATT repeat"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
     protein_bind    complement(10831..10834)
                     /label="BsmBI"
                     /ApEinfo_revcolor="#ff2f92"
                     /ApEinfo_fwdcolor="#ff2f92"
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0-0.5} {} 0} width 5 offset 0"
ORIGIN
        1 AGCCCTCGTT CACAGAGAAA GTTCGGTTAC ACTGCGGATT gacggatcgg gagatctttg
       61 tatttaatta agaccttgag tacgtgcgtc tctgcacgta tggcgattaa gaccttgagt
      121 acgtgcgtct ctgcacgtat ggcgattaag accttgagta cgtgcgtctc tgcacgtatg
      181 gcgattaaga ccttgagtac gtgcgtctct gcacgtatgg cgattaatcc atatgctcta
      241 gagggtatat aatgggggcc actagtctac taccagaaag cttggtaccg agctcggatc
      301 cagccaccAT Gggccctagt ggcccaATGG ACAAGAAGTA CTCCATTGGG CTCGATATCG
      361 GCACAAACAG CGTCGGCTGG GCCGTCATTA CGGACGAGTA CAAGGTGCCG AGCAAAAAAT
      421 TCAAAGTTCT GGGCAATACC GATCGCCACA GCATAAAGAA GAACCTCATT GGCGCCCTCC
      481 TGTTCGACTC CGGGGAGACG GCCGAAGCCA CGCGGCTCAA AAGAACAGCA CGGCGCAGAT
      541 ATACCCGCAG AAAGAATCGG ATCTGCTACC TGCAGGAGAT CTTTAGTAAT GAGATGGCTA
      601 AGGTGGATGA CTCTTTCTTC CATAGGCTGG AGGAGTCCTT TTTGGTGGAG GAGGATAAAA
      661 AGCACGAGCG CCACCCAATC TTTGGCAATA TCGTGGACGA GGTGGCGTAC CATGAAAAGT
      721 ACCCAACCAT ATATCATCTG AGGAAGAAGC TTGTAGACAG TACTGATAAG GCTGACTTGC
      781 GGTTGATCTA TCTCGCGCTG GCGCATATGA TCAAATTTCG GGGACACTTC CTCATCGAGG
      841 GGGACCTGAA CCCAGACAAC AGCGATGTCG ACAAACTCTT TATCCAACTG GTTCAGACTT
      901 ACAATCAGCT TTTCGAAGAG AACCCGATCA ACGCATCCGG AGTTGACGCC AAAGCAATCC
      961 TGAGCGCTAG GCTGTCCAAA TCCCGGCGGC TCGAAAACCT CATCGCACAG CTCCCTGGGG
     1021 AGAAGAAGAA CGGCCTGTTT GGTAATCTTA TCGCCCTGTC ACTCGGGCTG ACCCCCAACT
     1081 TTAAATCTAA CTTCGACCTG GCCGAAGATG CCAAGCTTCA ACTGAGCAAA GACACCTACG
     1141 ATGATGATCT CGACAATCTG CTGGCCCAGA TCGGCGACCA GTACGCAGAC CTTTTTTTGG
     1201 CGGCAAAGAA CCTGTCAGAC GCCATTCTGC TGAGTGATAT TCTGCGAGTG AACACGGAGA
     1261 TCACCAAAGC TCCGCTGAGC GCTAGTATGA TCAAGCGCTA TGATGAGCAC CACCAAGACT
     1321 TGACTTTGCT GAAGGCCCTT GTCAGACAGC AACTGCCTGA GAAGTACAAG GAAATTTTCT
     1381 TCGATCAGTC TAAAAATGGC TACGCCGGAT ACATTGACGG CGGAGCAAGC CAGGAGGAAT
     1441 TTTACAAATT TATTAAGCCC ATCTTGGAAA AAATGGACGG CACCGAGGAG CTGCTGGTAA
     1501 AGCTTAACAG AGAAGATCTG TTGCGCAAAC AGCGCACTTT CGACAATGGA AGCATCCCCC
     1561 ACCAGATTCA CCTGGGCGAA CTGCACGCTA TCCTCAGGCG GCAAGAGGAT TTCTACCCCT
     1621 TTTTGAAAGA TAACAGGGAA AAGATTGAGA AAATCCTCAC ATTTCGGATA CCCTACTATG
     1681 TAGGCCCCCT CGCCCGGGGA AATTCCAGAT TCGCGTGGAT GACTCGCAAA TCAGAAGAGA
     1741 CCATCACTCC CTGGAACTTC GAGGAAGTCG TGGATAAGGG GGCCTCTGCC CAGTCCTTCA
     1801 TCGAAAGGAT GACTAACTTT GATAAAAATC TGCCTAACGA AAAGGTGCTT CCTAAACACT
     1861 CTCTGCTGTA CGAGTACTTC ACAGTTTATA ACGAGCTCAC CAAGGTCAAA TACGTCACAG
     1921 AAGGGATGAG AAAGCCAGCA TTCCTGTCTG GAGAGCAGAA GAAAGCTATC GTGGACCTCC
     1981 TCTTCAAGAC GAACCGGAAA GTTACCGTGA AACAGCTCAA AGAAGACTAT TTCAAAAAGA
     2041 TTGAATGTTT CGACTCTGTT GAAATCAGCG GAGTGGAGGA TCGCTTCAAC GCATCCCTGG
     2101 GAACGTATCA CGATCTCCTG AAAATCATTA AAGACAAGGA CTTCCTGGAC AATGAGGAGA
     2161 ACGAGGACAT TCTTGAGGAC ATTGTCCTCA CCCTTACGTT GTTTGAAGAT AGGGAGATGA
     2221 TTGAAGAACG CTTGAAAACT TACGCTCATC TCTTCGACGA CAAAGTCATG AAACAGCTCA
     2281 AGAGGCGCCG ATATACAGGA TGGGGGCGGC TGTCAAGAAA ACTGATCAAT GGGATCCGAG
     2341 ACAAGCAGAG TGGAAAGACA ATCCTGGATT TTCTTAAGTC CGATGGATTT GCCAACCGGA
     2401 ACTTCATGCA GTTGATCCAT GATGACTCTC TCACCTTTAA GGAGGACATC CAGAAAGCAC
     2461 AAGTTTCTGG CCAGGGGGAC AGTCTTCACG AGCACATCGC TAATCTTGCA GGTAGCCCAG
     2521 CTATCAAAAA GGGAATACTG CAGACCGTTA AGGTCGTGGA TGAACTCGTC AAAGTAATGG
     2581 GAAGGCATAA GCCCGAGAAT ATCGTTATCG AGATGGCCCG AGAGAACCAA ACTACCCAGA
     2641 AGGGACAGAA GAACAGTAGG GAAAGGATGA AGAGGATTGA AGAGGGTATA AAAGAACTGG
     2701 GGTCCCAAAT CCTTAAGGAA CACCCAGTTG AAAACACCCA GCTTCAGAAT GAGAAGCTCT
     2761 ACCTGTACTA CCTGCAGAAC GGCAGGGACA TGTACGTGGA TCAGGAACTG GACATCAATC
     2821 GGCTCTCCGA CTACGACGTG GATCATATCG TGCCCCAGTC TTTTCTCAAA GATGATTCTA
     2881 TTGATAATAA AGTGTTGACA AGATCCGATA AAAATAGAGG GAAGAGTGAT AACGTCCCCT
     2941 CAGAAGAAGT TGTCAAGAAA ATGAAAAATT ATTGGCGGCA GCTGCTGAAC GCCAAACTGA
     3001 TCACACAACG GAAGTTCGAT AATCTGACTA AGGCTGAACG AGGTGGCCTG TCTGAGTTGG
     3061 ATAAAGCCGG CTTCATCAAA AGGCAGCTTG TTGAGACACG CCAGATCACC AAGCACGTGG
     3121 CCCAAATTCT CGATTCACGC ATGAACACCA AGTACGATGA AAATGACAAA CTGATTCGAG
     3181 AGGTGAAAGT TATTACTCTG AAGTCTAAGC TGGTCTCAGA TTTCAGAAAG GACTTTCAGT
     3241 TTTATAAGGT GAGAGAGATC AACAATTACC ACCATGCGCA TGATGCCTAC CTGAATGCAG
     3301 TGGTAGGCAC TGCACTTATC AAAAAATATC CCAAGCTTGA ATCTGAATTT GTTTACGGAG
     3361 ACTATAAAGT GTACGATGTT AGGAAAATGA TCGCAAAGTC TGAGCAGGAA ATAGGCAAGG
     3421 CCACCGCTAA GTACTTCTTT TACAGCAATA TTATGAATTT TTTCAAGACC GAGATTACAC
     3481 TGGCCAATGG AGAGATTCGG AAGCGACCAC TTATCGAAAC AAACGGAGAA ACAGGAGAAA
     3541 TCGTGTGGGA CAAGGGTAGG GATTTCGCGA CAGTCCGGAA GGTCCTGTCC ATGCCGCAGG
     3601 TGAACATCGT TAAAAAGACC GAAGTACAGA CCGGAGGCTT CTCCAAGGAA AGTATCCTCC
     3661 CGAAAAGGAA CAGCGACAAG CTGATCGCAC GCAAAAAAGA TTGGGACCCC AAGAAATACG
     3721 GCGGATTCGA TTCTCCTACA GTCGCTTACA GTGTACTGGT TGTGGCCAAA GTGGAGAAAG
     3781 GGAAGTCTAA AAAACTCAAA AGCGTCAAGG AACTGCTGGG CATCACAATC ATGGAGCGAT
     3841 CAAGCTTCGA AAAAAACCCC ATCGACTTTC TCGAGGCGAA AGGATATAAA GAGGTCAAAA
     3901 AAGACCTCAT CATTAAGCTT CCCAAGTACT CTCTCTTTGA GCTTGAAAAC GGCCGGAAAC
     3961 GAATGCTCGC TAGTGCGGGC GAGCTGCAGA AAGGTAACGA GCTGGCACTG CCCTCTAAAT
     4021 ACGTTAATTT CTTGTATCTG GCCAGCCACT ATGAAAAGCT CAAAGGGTCT CCCGAAGATA
     4081 ATGAGCAGAA GCAGCTGTTC GTGGAACAAC ACAAACACTA CCTTGATGAG ATCATCGAGC
     4141 AAATAAGCGA ATTCTCCAAA AGAGTGATCC TCGCCGACGC TAACCTCGAT AAGGTGCTTT
     4201 CTGCTTACAA TAAGCACAGG GATAAGCCCA TCAGGGAGCA GGCAGAAAAC ATTATCCACT
     4261 TGTTTACTCT GACCAACTTG GGCGCGCCTG CAGCCTTCAA GTACTTCGAC ACCACCATAG
     4321 ACAGAAAGCG GTACACCTCT ACAAAGGAGG TCCTGGACGC CACACTGATT CATCAGTCAA
     4381 TTACGGGGCT CTATGAAACA AGAATCGACC TCTCTCAGCT CGGTGGAGAC AGCAGGGCTG
     4441 ACCCCAAGAA GAAGAGGAAG GTGGGCGGGG GAGGATCCGA ATTCAAGTTG GAATTGGTAG
     4501 AAAAACTTTT TGCTGAAGAC ACAGAAGCAA AGAACCCATT TTCTACTCAG GACACAGATT
     4561 TAGACTTGGA GATGTTAGCT CCCTATATCC CAATGGATGA TGACTTCCAG TTACGTTCCT
     4621 TCGATCAGTT GTCACCATTA GAAAGCAGTT CCGCAAGCCC TGAAAGCGCA AGTCCTCAAA
     4681 GCACAGTTAC AGTATTCCAG CAGACTCAAA TACAAGAACC TACTGCTAAT GCCACCACTA
     4741 CCACTGCCAC CACTGATGAA TTAAAAACAG TGACAAAAGA CCGTATGGAA GACATTAAAA
     4801 TATTGATTGC ATCTCCATCT CCTACCCACA TACATAAAGA AACTACTTCT GGTGGTTCTC
     4861 CCAAGAAGAA GAGGAAAGTC ggcagcggcg agggcagagg cagcctgctg acctgcggcg
     4921 acgtggagga gaaccccggc cccatggatc caccacgagc gtcccacttg agccctcgga
     4981 agaagagacc ccggcagacg ggtgccttga tggcctcctc tcctcaagac atcaaatttc
     5041 aagatttggt cgtcttcatt ttggagaaga aaatgggaac cacccgcaga gcgttcctca
     5101 tggagctggc ccgcaggaaa gggttcaggg ttgaaaatga gctcagtgat tctgtcaccc
     5161 acattgtagc agagaacaac tcgggttcgg atgttctgga gtggcttcaa gcacagaaag
     5221 tacaagtcag ctcacaacca gagctcctcg atgtctcctg gctgatcgaa tgcataggag
     5281 cagggaaacc ggtggaaatg acaggaaaac accagcttgt tgtgagaaga gactattcag
     5341 atagcaccaa cccaggcccc ccgaagactc caccaattgc tgtacaaaag atctcccagt
     5401 atgcgtgtca gagaagaacc actttaaaca actgtaacca gatattcacg gatgcctttg
     5461 atatactggc tgaaaactgt gagtttagag aaaatgaaga ctcctgtgtg acatttatga
     5521 gagcagcttc tgtattgaaa tctctgccat tcacaatcat cagtatgaag gacacagaag
     5581 gaattccctg cctggggtcc aaggtgaagg gtatcataga ggagattatt gaagatggag
     5641 aaagttctga agttaaagct gtgttaaatg atgaacgata tcaatccttc aaactcttta
     5701 cttctgtatt tggagtgggg ctgaagactt ctgagaagtg gttcaggatg ggtttcagaa
     5761 ctctgagtaa agtaaggtcg gacaaaagcc tgaaatttac acgaatgcag aaagcaggat
     5821 ttctgtatta tgaagacctt gtcagctgtg tgaccagggc agaagcagag gccgtcagtg
     5881 tgctggttaa agaggctgtc tgggcatttc ttccggatgc tttcgtcacc atgacaggag
     5941 ggttccggag gggtaagaag atggggcatg atgtagattt tttaattacc agcccaggat
     6001 caacagagga tgaagagcaa cttttacaga aagtgatgaa cttatgggaa aagaagggat
     6061 tacttttata ttatgacctt gtggagtcaa catttgaaaa gctcaggttg cctagcagga
     6121 aggttgatGC Tatggatcat tttcaaaagt gctttctgat tttcaaattg cctcgtcaaa
     6181 gagtggacag tgaccagtcc agctggcagg aaggaaagac ctggaaggcc atccgtgtgg
     6241 atttagttct gtgcccctac gagcgtcgtg cctttgccct gttgggatgg actggAtccg
     6301 atCAGTTTga tAGAGACCTC cggcgctatg ccacacatga gcggaagatg attctggata
     6361 accatgcttt atatgacaag accaagagga tattcctcaa agcagaaagt gaagaagaaa
     6421 tttttgcgca tctgggattg gattatattg aaccgtggga aagaaatgcc tagTGAAAGG
     6481 GTTCGATCCC TACCGGTTAG TAATGAGTTT AAACGGGGGA GGCTAACTGA AACACGGAAG
     6541 GAGACAATAC CGGAAGGAAC CCGCGCTATG ACGGCAATAA AAAGACAGAA TAAAACGCAC
     6601 GGGTGTTGGG TCGTTTGTTC ATAAACGCGG GGTTCGGTCC CAGGGCTGGa gcagttacag
     6661 agatgttacg aaccctgaac aggttgacta ttaattgtgt ctgcttgatg tggacaccag
     6721 gtggcgctgg acatcagatt tggagaggca gttgtctagg gaaccgggct ctgtgccagc
     6781 gcaggaggca ggctggctct cctattccag ggatgctcat ccaggaagga aaggttgcat
     6841 gctggacaca ctaaccttac aGCGGTGGGC TCTATGGGGT ACCTACGTAT AGATCTTGAG
     6901 ACAAATGGCA GTATTCATCC ACAATTTTAA AAGAAAAGGG GGGATTGGGG GGTACAGTGC
     6961 AGGGGAAAGA ATAGTAGACA TAATAGCAAC AGACATACAA ACTAAAGAAT TACAAAAACA
     7021 AATTACAAAA ATTCAAAATT TTCGGGTTTA TTACAGGGAC AGCAGAGATC CACTTTGGCG
     7081 CCGGCTCGAG TGGCTCCGGT GCCCGTCAGT GGGCAGAGCG CACATCGCCC ACAGTCCCCG
     7141 AGAAGTTGGG GGGAGGGGTC GGCAATTGAA CCGGTGCCTA GAGAAGGTGG CGCGGGGTAA
     7201 ACTGGGAAAG TGATGTCGTG TACTGGCTCC GCCTTTTTCC CGAGGGTGGG GGAGAACCGT
     7261 ATATAAGTGC AGTAGTCGCC GTGAACGTTC TTTTTCGCAA CGGGTTTGCC GCCAGAACAC
     7321 AGGTGTCGTG ACGCGGGATC CGCCACCatg gtgagcaagg gcgaggagga taacatggcc
     7381 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     7441 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     7501 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     7561 tacggctcca aggcctacgt gaagcacccc gccgacattc ccgactactt gaagctgtcc
     7621 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     7681 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     7741 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     7801 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     7861 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     7921 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     7981 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     8041 gacgagctgt acaagtctac agatgtcgaA TCCTAACTCG AGCGACGCCT CGACTGTGCC
     8101 TTCTAGTTGC CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCTTCCTTGA CCCTGGAAGG
     8161 TGCCACTCCC ACTGTCCTTT CCTAATAAAA TGAGGAAATT GCATCGCATT GTCTGAGTAG
     8221 GTGTCATTCT ATTCTGGGGG GTGGGGTGGG GCAGGACAGC AAGGGGGAGG ATTGGGAAGA
     8281 CAATAGCAGG CATGCTGGGG ATGCGGTGGG CTCTATGAGT atcgatacta gttaaaagtt
     8341 ttgttacttt atagaagaaa ttttgagttt ttgttttttt ttaataaata aataaacata
     8401 aataaattgt ttgttgaatt tattattagt atgtaagtgt aaatataata aaacttaata
     8461 tctattcaaa ttaataaata aacctcgata tacagaccga taaaacacat gcgtcaattt
     8521 tacgcatgat tatctttaac gtacgtcaca atatgattat ctttctaggg ttaatctagt
     8581 atacgcgtat gcggccgctt aattaatcca gcttttgttc cctttagtga gggttaattC
     8641 CGAgcggccg cgtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     8701 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
     8761 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     8821 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     8881 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     8941 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     9001 tcactcgcat caaccaaacc Attattcatt cgtgattgcg cctgagcgag gcgaaatacg
     9061 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     9121 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     9181 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     9241 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     9301 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     9361 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     9421 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     9481 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     9541 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     9601 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agtcatgacc aaaatccctt
     9661 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9721 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9781 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9841 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9901 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9961 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    10021 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    10081 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    10141 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    10201 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10261 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10321 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10381 tatcccctga ttctgtggat aaccgtgcgg ccgccaatta atacgactca ctatagggcg
    10441 aattggggcg cgccattcta gattaaccct agaaagatag tctgcgtaaa attgacgcat
    10501 gcattcttga aatattgctc tctctttcta aatagcgcga atccgtcgct gtgcatttag
    10561 gacatctcag tcgccgcttg gagctcccgt gaggcgtgct tgtcaatgcg gtaagtgtca
    10621 ctgattttga actataacga ccgcgtgagt caaaatgacg catgattatc ttttacgtga
    10681 cttttaagat ttaactcata cgataattat attgttattt catgttctac ttacgtgata
    10741 acttattata tatatatttt cttgttatag atatcaacta gaatgctagc atgggcccag
    10801 atctccatgg gCCCTgaatt cgcatctaga ctga
//