LOCUS       pcmk74-wvo1-kan        2731 bp DNA     circular SYN 07-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 7, 2024 from SnapGene Viewer 6.2.0
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      64..150
                     /label=rrnB T1 terminator
     misc_feature    151..1693
                     /label=wvo1 ori
     misc_feature    complement(171..869)
                     /label=repA
     CDS             complement(171..869)
                     /codon_start=1
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     misc_feature    complement(936..1097)
                     /label=orf c
     misc_feature    complement(1138..1347)
                     /label=orfB
     misc_feature    1300..1348
                     /label=inverted repeat 3
     misc_feature    1432..1454
                     /label=inverted repeat 2
     primer_bind     1454..1473
                     /label=ck273 pbav1k seq F
     misc_feature    1501..1632
                     /label=inverted repeat 1
     terminator      1694..1788
                     /label=lambda t0 terminator
     misc_feature    1792..1799
                     /label=hip1
     CDS             complement(1806..2600)
                     /label=APH (KanR)
     CDS             complement(1806..2600)
                     /codon_start=1
                     /translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
                     EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    complement(2601..2724)
                     /label=promoter
     misc_feature    complement(2653..2661)
                     /label=-10
     misc_feature    complement(2674..2679)
                     /label=-35
     misc_feature    complement(2688..2695)
                     /label=rpod16
ORIGIN
        1 ttttcaaagt tggcgtataa catagtatcg acggagccga ttttgaaacc acaccagagg
       61 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
      121 tcggtgaacg ctctcctgag taggacaaat ccgccgccct agacctagtg tcattttatt
      181 tcccccgttt cagcatcaag aacctttgca taacttgctc tatatccaca ctgataattg
      241 ccctcaaacc ataatctaaa ggcgctagag tttgttgaaa caatatcttt tacatcattc
      301 gtatttaaaa ttccaaactc cgctccccta aggcgaataa aagccattaa atcttttgta
      361 tttaccaaat tatagtcatc cactatatct aagagtaaat tcttcaattc tcttttttgg
      421 ctttcatcaa gtgttatata gcggtcaata tcaaaatcat taatgttcaa aatatctttt
      481 ttgtcgtata tatgtttatt cttagcaata gcgtcctttg attcatgagt caaatattca
      541 tatgaacctt tgatataatc aagtatctca acatgagcaa ctgaactatt ccccaatttt
      601 cgcttaatct tgttcctaac gctttctatt gttacaggat ttcgtgcaat atatataacg
      661 tgatagtgtg gttttttata gtgctttcca tttcgtataa catcactact attccatgta
      721 tctttatctt ttttttcgtc catatcgtgt aaaggactga cagccataga tacgcccaaa
      781 ctctctaatt tttccttcca atcattagga attgagtcag gatataataa aaatccaaaa
      841 tttctagctt tagtattttt aatagccatg atataattac cttatcaaaa acaagtagcg
      901 aaaactcgta tccttctaaa aacgcgagct ttcgcttatt ttttttgttc tgattccttt
      961 cttgcatatt cttctatagc taacgccgca accgcagatt ttgaaaaacc tttttgtttc
     1021 gccatatctg ttaatttttt atcttgctct tttgtcagag aaatcataac tctttttttc
     1081 gattctgaaa tcaccattta aaaaactcca atcaaataat tttataaagt tagtgtatca
     1141 ctttgtaatc ataaaaacaa caataaagct acttaaatat agatttataa aaaacgttgg
     1201 cgaaaacgtt ggcgattcgt tggcgattga aaaacccctt aaacccttga gccagttggg
     1261 atagagcgtt tttggcacaa aaattggcac tcggcactta atggggggtc gtagtacgga
     1321 agcaaaattc gcttcctttc cccccatttt tttccaaatt ccagattttt ttcaaaaatt
     1381 ttccagcgct accgctcggc aaaattgcaa gcaattttta aaatcaaacc catgagggaa
     1441 tttcattccc tcatactccc ttgagcctcc tccaaccgaa atagaagggc gctgcgctta
     1501 ttatttcatt cagtcatcgg ctttcataat ctaacagaca acatcttcgc tgcaaagcca
     1561 cgctacgctc aagggctttt acgctacgat aacgcctgtt ttaacgatta tgccgataac
     1621 taaacgaaat aaacgctaaa acgtctcaga aacgattttg agacgtttta ataaaaaatc
     1681 gcctagtgct tggattctca ccaataaaaa acgcccggcg gcaaccgagc gttctgaaca
     1741 aatccagatg gagttctgag gtcattactg gatctatcaa cgggagtcca agcgatcgct
     1801 cggtactaaa acaattcatc cagtaaaata taatatttta ttttctccca atcaggcttg
     1861 atccccagta agtcaaaaaa tagctcgaca tactgttctt ccccgatatc ctccctgatc
     1921 gaccggacgc agaaggcaat gtcataccac ttgtccgccc tgccgcttct cccaagatca
     1981 ataaagccac ttactttgcc atctttcaca aagatgttgc tgtctcccag gtcgccgtgg
     2041 gaaaagacaa gttcctcttc gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga
     2101 tctttaaatg gagtgtcttc ttcccagttt tcgcaatcca catcggccag atcgttattc
     2161 agtaagtaat ccaattcggc taagcggccg tctaagctat tcgtataggg acaatccgat
     2221 atgtcgatgg agtgaaagag cctgatgcac tccgcataca gctcgatagt cttttcaggg
     2281 ctttgttcat cttcataccc ttccgagcaa aggacgccat cggcctcact catgagcaga
     2341 ttgctccagc catcatgccg ttcaaagtgc aggacctttg gaacaggcag ctttccttcc
     2401 agccatagca tcatgtcctt ttcccgttcc acatcatagg tggtcccttt ataccggctg
     2461 tccgtcattt ttaaatatag gatttcattt tctcccacca gcttatatac cttagcagga
     2521 gacattcctt ccgtatcttt tacgcagcgg tattcttcga tcagtttttt caattccggt
     2581 gatattctca ttttagccat ttattatttc cttcctcttt tctacagtat ttaaagatac
     2641 cccaagaagc taattataac aagacgaact ccaattcact gttccttgca ttctaaaacc
     2701 ttaaatacag aaaacagcct tttcaaagtt g
//
