LOCUS       pTBL2142_MTK3_ne        2376 bp    DNA        circular     09-AUG-2024
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    606..609
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1321..1323)
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    599..604
                     /locus_tag="BsaI(2)"
                     /label="BsaI(2)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1325..1330)
                     /locus_tag="BsaI(3)"
                     /label="BsaI(3)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(join(2207..2376,1..594))
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2102..2206)
                     /locus_tag="CamR Promoter"
                     /label="CamR Promoter"
                     /ApEinfo_label="CamR Promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1442..2101)
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_label="CamR"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1333..1441)
                     /locus_tag="CamR Terminator"
                     /label="CamR Terminator"
                     /ApEinfo_label="CamR Terminator"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             610..1320
                     /locus_tag="sfGFP"
                     /label="sfGFP"
                     /ApEinfo_label="sfGFP"
                     /ApEinfo_fwdcolor="#34FF03"
                     /ApEinfo_revcolor="#34FF03"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
ORIGIN
        1 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
       61 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      601 tctcatatgc gtaaaggcga agagctgttc actggtgtcg tccctattct ggtggaactg
      661 gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg tgacgcaact
      721 aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt tccttggccg
      781 actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc ggaccatatg
      841 aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga acgcacgatt
      901 tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga aggcgatacc
      961 ctggtaaacc gcattgagct gaaaggcatt gactttaaag aggacggcaa tatcctgggc
     1021 cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga taaacaaaaa
     1081 aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag cgtgcagctg
     1141 gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct gccagacaat
     1201 cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg cgatcatatg
     1261 gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga actgtacaaa
     1321 tcctgagacc agaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     1381 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgatatcaa
     1441 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga
     1501 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt
     1561 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg
     1621 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgaaacg aaaaacatat
     1681 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg
     1741 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg
     1801 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct
     1861 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt
     1921 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa
     1981 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat
     2041 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca
     2101 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc
     2161 ttatttcatt atggtgaaag ttggaacctc ttacgtgccc gatcaatcat gaccaaaatc
     2221 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2281 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2341 ccagcggtgg tttgtttgcc ggatcaagag ctacca  
//
