LOCUS       pTBL3346_MTK_PB_        4982 bp    DNA        circular     08-SEP-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     pTBL3341 MTK_TU-cHS4-hU6-nicking guide from 169 (Esp3I) to 1299
            (Esp3I) 
COMMENT     pCKC517 MTK0-PB-pEF1ashort-blastR-noBsaI-noextraEsp3I from 1339
            (Esp3I) to 14 (Esp3I) 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    893..903
                     /locus_tag="modified stem loop"
                     /label="modified stem loop"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1157..1339
                     /locus_tag="Insulator A1"
                     /label="Insulator A1"
                     /ApEinfo_label="Insulator A1"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..25
                     /locus_tag="ConS scar"
                     /label="ConS scar"
                     /ApEinfo_label="ConS scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2679..2709
                     /locus_tag="31bp spacer"
                     /label="31bp spacer"
                     /ApEinfo_label="31bp spacer"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    323..602
                     /locus_tag="HS4"
                     /label="HS4"
                     /ApEinfo_label="HS4"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    622..871
                     /locus_tag="human U6 promoter"
                     /label="human U6 promoter"
                     /ApEinfo_label="human U6 promoter"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     protein_bind    618..621
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    905..916
                     /locus_tag="modified stem loop(1)"
                     /label="modified stem loop(1)"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    918..984
                     /locus_tag="modified stem loop(2)"
                     /label="modified stem loop(2)"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    985..988
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     Terminator      2235..2468
                     /locus_tag="BGH-PA"
                     /label="BGH-PA"
                     /ApEinfo_label="BGH-PA"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4635..4653
                     /locus_tag="19bp IR"
                     /label="19bp IR"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4803..4808
                     /locus_tag="CAAAT repeat"
                     /label="CAAAT repeat"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1..4
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    40..319
                     /locus_tag="HS4(1)"
                     /label="HS4(1)"
                     /ApEinfo_label="HS4"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    989..1131
                     /locus_tag="ConE'"
                     /label="ConE'"
                     /ApEinfo_label="ConE'"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2710..2722
                     /locus_tag="13bp TR"
                     /label="13bp TR"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4571..4590
                     /locus_tag="T7 Forward (Elim)"
                     /label="T7 Forward (Elim)"
                     /ApEinfo_label="T7 Forward (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1372..1829
                     /locus_tag="truncated pEF1a"
                     /label="truncated pEF1a"
                     /ApEinfo_label="truncated pEF1a"
                     /ApEinfo_fwdcolor="#00f900"
                     /ApEinfo_revcolor="#00f900"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    1132..1156
                     /locus_tag="ConE scar"
                     /label="ConE scar"
                     /ApEinfo_label="ConE scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2660..2678
                     /locus_tag="19bp IR(1)"
                     /label="19bp IR(1)"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2772..2791)
                     /locus_tag="T3 (Elim)"
                     /label="T3 (Elim)"
                     /ApEinfo_label="T3 (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        2485..2732
                     /locus_tag="3' PB repeat"
                     /label="3' PB repeat"
                     /ApEinfo_label="3' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2512..2517)
                     /locus_tag="CAAAAT repeat"
                     /label="CAAAAT repeat"
                     /ApEinfo_label="CAAAAT repeat"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      complement(2804..2830)
                     /locus_tag="KanR Terminator"
                     /label="KanR Terminator"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(2773..2791)
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(3795..4558)
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        4615..4936
                     /locus_tag="5' PB repeat"
                     /label="5' PB repeat"
                     /ApEinfo_label="5' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1132..1135
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4619..4631
                     /locus_tag="13bp TR(1)"
                     /label="13bp TR(1)"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(4776..4781)
                     /locus_tag="CAAAT repeat(1)"
                     /label="CAAAT repeat(1)"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(2831..3646)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    4893..4899
                     /locus_tag="ACTTATT repeat"
                     /label="ACTTATT repeat"
                     /ApEinfo_label="ACTTATT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(3647..3794)
                     /locus_tag="KanR Promoter"
                     /label="KanR Promoter"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1830..2228
                     /locus_tag="blast"
                     /label="blast"
                     /ApEinfo_label="blast"
                     /ApEinfo_fwdcolor="#fb0022"
                     /ApEinfo_revcolor="#fb0022"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    3173..3173
                     /locus_tag="SNP from cloning (doesn't affect kanR)"
                     /label="SNP from cloning (doesn't affect kanR)"
                     /ApEinfo_label="SNP from cloning (doesn't affect kanR)"
                     /ApEinfo_fwdcolor="#ff2600"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctgaactggc cgataattgc agacgATCGA ATTCGAGTTG GCGCGCCTGT CATTCTAAAT
       61 CTCTCTTTCA GCCTAAAGCT TTTTCCCCGT ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG
      121 CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT TCCCCGTGCC CGGGCTGTCC
      181 CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG
      241 GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA TCCCTGGGGG CTTTGGGAGG
      301 GGGCTGTCCC CGTGAGCTCC CAGGCGCGCC TGTCATTCTA AATCTCTCTT TCAGCCTAAA
      361 GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA GAGCAGCGAG AAGCGTTCAG
      421 AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG TCCCCGCACG CTGCCGGCTC
      481 GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG GCGGCTCGCT GCTGCCCCCT
      541 AGCGGGGGAG GGACGTAATT ACATCCCTGG GGGCTTTGGG GGGGGGCTGT CCCCGTGAGC
      601 TCAATAAAAG AGCCCACaac gGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA
      661 CGATACAAGG CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA
      721 GTACAAAATA CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA
      781 TGTTTTAAAA TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT
      841 TTATATATCT TGTGGAAAGG ACGAAACACC gactccagtc tttctagaag aGTTTAAGAG
      901 CTAAGCTGGA AACAGCATAG CAAGTTTAAA TAAGGCTAGT CCGTTATCAA CTTGAAAAAG
      961 TGGCACCGAG TCGGTGCTTT TTTTgctgga aatctgctcg tcagtggtgc tcacactgac
     1021 gaatcatgta cagatcatac cgatgactgc ctggcgactc acaactaagc aagacagccg
     1081 gaaccagcgc cggcgaacac cactgcatat atggcatatc acaacagtcc aagcagttac
     1141 agagatgtta cgaaccctga acaggttgac tattaattgt gtctgcttga tgtggacacc
     1201 aggtggcgct ggacatcaga tttggagagg cagttgtcta gggaaccggg ctctgtgcca
     1261 gcgcaggagg caggctggct ctcctattcc agggatgctc atccaggaag gaaaggttgc
     1321 atgctggaca cactaacctt acaGCGGTGG GCTCTATGGG GTACCTACGT ATAGATCTTG
     1381 AGACAAATGG CAGTATTCAT CCACAATTTT AAAAGAAAAG GGGGGATTGG GGGGTACAGT
     1441 GCAGGGGAAA GAATAGTAGA CATAATAGCA ACAGACATAC AAACTAAAGA ATTACAAAAA
     1501 CAAATTACAA AAATTCAAAA TTTTCGGGTT TATTACAGGG ACAGCAGAGA TCCACTTTGG
     1561 CGCCGGCTCG AGTGGCTCCG GTGCCCGTCA GTGGGCAGAG CGCACATCGC CCACAGTCCC
     1621 CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACCGGTGCC TAGAGAAGGT GGCGCGGGGT
     1681 AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT CCCGAGGGTG GGGGAGAACC
     1741 GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC AACGGGTTTG CCGCCAGAAC
     1801 ACAGGTGTCG TGACGCGGGA TCCGCCACCA TGGCCAAGCC TTTGTCTCAA GAAGAATCCA
     1861 CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA GACTACAGCG
     1921 TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT GTATATCATT
     1981 TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT GCGGCAGCTG
     2041 GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG AGCCCCTGCG
     2101 GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA GTGAAGGACA
     2161 GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT TATGTGTGGG
     2221 AGGGCTAACT CGAGCGACGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
     2281 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
     2341 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
     2401 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG
     2461 GGCTCTATGA GTatcgatac tagttaaaag ttttgttact ttatagaaga aattttgagt
     2521 ttttgttttt ttttaataaa taaataaaca taaataaatt gtttgttgaa tttattatta
     2581 gtatgtaagt gtaaatataa taaaacttaa tatctattca aattaataaa taaacctcga
     2641 tatacagacc gataaaacac atgcgtcaat tttacgcatg attatcttta acgtacgtca
     2701 caatatgatt atctttctag ggttaatcta gtatacgcgt atgcggccgc ttaattaatc
     2761 cagcttttgt tccctttagt gagggttaat tCCGAgcggc cgcgtgttac aaccaattaa
     2821 ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag
     2881 gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga
     2941 ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat
     3001 caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat
     3061 gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc cagacttgtt
     3121 caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccAttattca
     3181 ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga caattacaaa
     3241 caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg
     3301 aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta
     3361 accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg
     3421 tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat
     3481 gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg
     3541 attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat
     3601 ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat
     3661 tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta tcttgtgcaa
     3721 tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga acttttgctg
     3781 agttgaagga tcagtcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3841 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3901 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3961 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     4021 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     4081 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     4141 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     4201 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     4261 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4321 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4381 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4441 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4501 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtgc
     4561 ggccgccaat taatacgact cactataggg cgaattgggg cgcgccattc tagattaacc
     4621 ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc tctctctttc
     4681 taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct tggagctccc
     4741 gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac gaccgcgtga
     4801 gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca tacgataatt
     4861 atattgttat ttcatgttct acttacgtga taacttatta tatatatatt ttcttgttat
     4921 agatatcaac tagaatgcta gcatgggccc agatctccat gggCCCTgaa ttcgcatcta
     4981 ga     
//
