LOCUS       pTBL3241_MTK_PB_        6000 bp    DNA        circular     08-SEP-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     pTBL3063 MTK-TU hU6-AtoB-ACC-in-gen_Cas9_nick from 169 (Esp3I) to
            885 (Esp3I) 
COMMENT     pTBL3240 Con5toCon7 from 169 (Esp3I) to 220 (Esp3I) 
COMMENT     pTBL2537 MTK-pEF1a-mCherry-BGHpA from 169 (Esp3I) to 2651 (Esp3I) 
COMMENT     pTBL2367 MTK0-PB-nohyg from 1339 (Esp3I) to 14 (Esp3I) 
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    537..547
                     /locus_tag="modified stem loop"
                     /label="modified stem loop"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    718..721
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    263..266
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    622..655
                     /locus_tag="34ntextension"
                     /label="34ntextension"
                     /ApEinfo_label="34ntextension"
                     /ApEinfo_fwdcolor="#fc66fe"
                     /ApEinfo_revcolor="#fc66fe"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    26..262
                     /locus_tag="E4 Insulator"
                     /label="E4 Insulator"
                     /ApEinfo_label="E4 Insulator"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..25
                     /locus_tag="ConS scar"
                     /label="ConS scar"
                     /ApEinfo_label="ConS scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    267..515
                     /locus_tag="human U6 promoter"
                     /label="human U6 promoter"
                     /ApEinfo_label="human U6 promoter"
                     /ApEinfo_fwdcolor="#00ff00"
                     /ApEinfo_revcolor="#00ff00"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    533..535
                     /locus_tag="site3sgRNA"
                     /label="site3sgRNA"
                     /ApEinfo_label="site3sgRNA"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    747..765
                     /locus_tag="bu6 Promoter"
                     /label="bu6 Promoter"
                     /ApEinfo_label="bu6 Promoter"
                     /ApEinfo_fwdcolor="#faac61"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    718..742
                     /locus_tag="Con5 scar"
                     /label="Con5 scar"
                     /ApEinfo_label="Con5 scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1..4
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    549..560
                     /locus_tag="modified stem loop(1)"
                     /label="modified stem loop(1)"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    562..621
                     /locus_tag="modified stem loop(2)"
                     /label="modified stem loop(2)"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    743..746
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    676..686
                     /locus_tag="longpeg"
                     /label="longpeg"
                     /ApEinfo_label="longpeg"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    676..686
                     /locus_tag="HIS6-insertion"
                     /label="HIS6-insertion"
                     /ApEinfo_label="HIS6-insertion"
                     /ApEinfo_fwdcolor="#72fcd5"
                     /ApEinfo_revcolor="#72fcd5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(516..532)
                     /locus_tag="HIS6-insertion(1)"
                     /label="HIS6-insertion(1)"
                     /ApEinfo_label="HIS6-insertion"
                     /ApEinfo_fwdcolor="#72fcd5"
                     /ApEinfo_revcolor="#72fcd5"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    complement(516..532)
                     /locus_tag="longpeg(1)"
                     /label="longpeg(1)"
                     /ApEinfo_label="longpeg"
                     /ApEinfo_fwdcolor="#ff84ff"
                     /ApEinfo_revcolor="#ff84ff"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    687..690
                     /locus_tag="modified stem loop(3)"
                     /label="modified stem loop(3)"
                     /ApEinfo_label="modified stem loop"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    714..717
                     /locus_tag="BsaI(2)"
                     /label="BsaI(2)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    766..769
                     /locus_tag="BsaI(3)"
                     /label="BsaI(3)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1906..2060
                     /locus_tag="EF1Alpha"
                     /label="EF1Alpha"
                     /ApEinfo_label="EF1Alpha"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2124..2127
                     /locus_tag="BsaI(4)"
                     /label="BsaI(4)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    774..794
                     /locus_tag="Con7 Scar"
                     /label="Con7 Scar"
                     /ApEinfo_label="Con7 Scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    770..773
                     /locus_tag="BsmBI(2)"
                     /label="BsmBI(2)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    795..938
                     /locus_tag="B2 insulator"
                     /label="B2 insulator"
                     /ApEinfo_label="B2 insulator"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    943..1904
                     /locus_tag="EF1Alpha(1)"
                     /label="EF1Alpha(1)"
                     /ApEinfo_label="EF1Alpha"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2128..2846
                     /locus_tag="mCherry"
                     /label="mCherry"
                     /ApEinfo_label="mCherry"
                     /ApEinfo_fwdcolor="#ff3129"
                     /ApEinfo_revcolor="#ff3129"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     Terminator      2860..3095
                     /locus_tag="BGH-PA"
                     /label="BGH-PA"
                     /ApEinfo_label="BGH-PA"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    3096..3099
                     /locus_tag="BsaI(5)"
                     /label="BsaI(5)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3100..3242
                     /locus_tag="ConE'"
                     /label="ConE'"
                     /ApEinfo_label="ConE'"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3268..3450
                     /locus_tag="Insulator A1"
                     /label="Insulator A1"
                     /ApEinfo_label="Insulator A1"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3697..3727
                     /locus_tag="31bp spacer"
                     /label="31bp spacer"
                     /ApEinfo_label="31bp spacer"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5653..5671
                     /locus_tag="19bp IR"
                     /label="19bp IR"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5821..5826
                     /locus_tag="CAAAT repeat"
                     /label="CAAAT repeat"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3728..3740
                     /locus_tag="13bp TR"
                     /label="13bp TR"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5589..5608
                     /locus_tag="T7 Forward (Elim)"
                     /label="T7 Forward (Elim)"
                     /ApEinfo_label="T7 Forward (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3243..3267
                     /locus_tag="ConE scar"
                     /label="ConE scar"
                     /ApEinfo_label="ConE scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3678..3696
                     /locus_tag="19bp IR(1)"
                     /label="19bp IR(1)"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3790..3809)
                     /locus_tag="T3 (Elim)"
                     /label="T3 (Elim)"
                     /ApEinfo_label="T3 (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        3503..3750
                     /locus_tag="3' PB repeat"
                     /label="3' PB repeat"
                     /ApEinfo_label="3' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(3530..3535)
                     /locus_tag="CAAAAT repeat"
                     /label="CAAAAT repeat"
                     /ApEinfo_label="CAAAAT repeat"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      complement(3822..3848)
                     /locus_tag="KanR Terminator"
                     /label="KanR Terminator"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(3791..3809)
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(4813..5576)
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        5633..5954
                     /locus_tag="5' PB repeat"
                     /label="5' PB repeat"
                     /ApEinfo_label="5' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    3243..3246
                     /locus_tag="BsmBI(3)"
                     /label="BsmBI(3)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5637..5649
                     /locus_tag="13bp TR(1)"
                     /label="13bp TR(1)"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(5794..5799)
                     /locus_tag="CAAAT repeat(1)"
                     /label="CAAAT repeat(1)"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(3849..4664)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5911..5917
                     /locus_tag="ACTTATT repeat"
                     /label="ACTTATT repeat"
                     /ApEinfo_label="ACTTATT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             3477..3496
                     /locus_tag="sfGFP"
                     /label="sfGFP"
                     /ApEinfo_label="sfGFP"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(4665..4812)
                     /locus_tag="KanR Promoter"
                     /label="KanR Promoter"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3471..3476
                     /locus_tag="KpnI"
                     /label="KpnI"
                     /ApEinfo_label="KpnI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3497..3502
                     /locus_tag="SpeI"
                     /label="SpeI"
                     /ApEinfo_label="SpeI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    47..70
                     /locus_tag="nanopore-E4-F"
                     /label="nanopore-E4-F"
                     /ApEinfo_label="nanopore-E4-F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    865..884
                     /locus_tag="nanopore-B2-R"
                     /label="nanopore-B2-R"
                     /ApEinfo_label="nanopore-B2-R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    5..28
                     /locus_tag="ooE4-F"
                     /label="ooE4-F"
                     /ApEinfo_label="ooE4-F"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1006..1028
                     /locus_tag="oopEF1R"
                     /label="oopEF1R"
                     /ApEinfo_label="oopEF1R"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 ctgaactggc cgataattgc agacgatgtt tctatatgtt ttaagtctta ggcatatcgg
       61 acatcactag tgcatcagcg ccctctgctg gttcagtaag aatggtttcc ccatatactg
      121 ggcaaaactg gatttttgtg gtgatgaaag ggaaaaaaac aaattcagta caattggaaa
      181 gctggtgttg ttttaaaact cttgaaaaac actgaatgaa aggaatcaca ctaaaactat
      241 atgttgcaat gttgttggtt aaaacgGAGG GCCTATTTCC CATGATTCCT TCATATTTGC
      301 ATATACGATA CAAGGCTGTT AGAGAGATAA TTAGAATTAA TTTGACTGTA AACACAAAGA
      361 TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT GCAGTTTTAA
      421 AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT TTCGATTTCT
      481 TGGCTTTATA TATCTTGTGG AAAGGACGAA ACACCGCCAT CATCACCATC ATTGAGTTTA
      541 AGAGCTAAGC TGGAAACAGC ATAGCAAGTT TAAATAAGGC TAGTCCGTTA TCAACTTGAA
      601 AAAGTGGCAC CGAGTCGGTG CTGGAGGAAG CAGGGCTTCC TTTCCTCTGC CATCAATGAT
      661 AGATGGCCCA GTggtATGAT GGTGATTTTT ccatcttcta gaaagactgg agtgctgaag
      721 tgaataaagc tccacacagt cgaacgcgtg accgagcttg tctgcgctgg cgggcgacga
      781 tctcagaaca gttacaacac caggcttctg gctccagtcc ttggagtccg tctgcgtccc
      841 agcagctccc cctggtggcc agaagcaaac gcttcatgtc ttgcatctag agccaaagca
      901 tctcctaccc ctggattccc attccggaat agttttcaAA CGGTGCCCGT CAGTGGGCAG
      961 AGCGCACATC GCCCACAGTC CCCGAGAAGT TGGGGGGAGG GGTCGGCAAT TGAACCGGTG
     1021 CCTAGAGAAG GTGGCGCGGG GTAAACTGGG AAAGTGATGT CGTGTACTGG CTCCGCCTTT
     1081 TTCCCGAGGG TGGGGGAGAA CCGTATATAA GTGCAGTAGT CGCCGTGAAC GTTCTTTTTC
     1141 GCAACGGGTT TGCCGCCAGA ACACAGGTAA GTGCCGTGTG TGGTTCCCGC GGGCCTGGCC
     1201 TCTTTACGGG TTATGGCCCT TGCGTGCCTT GAATTACTTC CACCTGGCTG CAGTACGTGA
     1261 TTCTTGATCC CGAGCTTCGG GTTGGAAGTG GGTGGGAGAG TTCGAGGCCT TGCGCTTAAG
     1321 GAGCCCCTTC GCCTCGTGCT TGAGTTGAGG CCTGGCCTGG GCGCTGGGGC CGCCGCGTGC
     1381 GAATCTGGTG GCACCTTCGC GCCTGTCTCG CTGCTTTCGA TAAGTCTCTA GCCATTTAAA
     1441 ATTTTTGATG ACCTGCTGCG ACGCTTTTTT TCTGGCAAGA TAGTCTTGTA AATGCGGGCC
     1501 AAGATCTGCA CACTGGTATT TCGGTTTTTG GGGCCGCGGG CGGCGACGGG GCCCGTGCGT
     1561 CCCAGCGCAC ATGTTCGGCG AGGCGGGGCC TGCGAGCGCG GCCACCGAGA ATCGGACGGG
     1621 GGTAGTCTCA AGCTGGCCGG CCTGCTCTGG TGCCTGGCCT CGCGCCGCCG TGTATCGCCC
     1681 CGCCCTGGGC GGCAAGGCTG GCCCGGTCGG CACCAGTTGC GTGAGCGGAA AGATGGCCGC
     1741 TTCCCGGCCC TGCTGCAGGG AGCTCAAAAT GGAGGACGCG GCGCTCGGGA GAGCGGGCGG
     1801 GTGAGTCACC CACACAAAGG AAAAGGGCCT TTCCGTCCTC AGCCGTCGCT TCATGTGACT
     1861 CCACGGAGTA CCGGGCGCCG TCCAGGCACC TCGATTAGTT CTCGcGCTTT TGGAGTACGT
     1921 CGTCTTTAGG TTGGGGGGAG GGGTTTTATG CGATGGAGTT TCCCCACACT GAGTGGGTGG
     1981 AGACTGAAGT TAGGCCAGCT TGGCACTTGA TGTAATTCTC CTTGGAATTT GCCCTTTTTG
     2041 AGTTTGGATC TTGGTTCATT CTCAAGCCTC AGACAGTGGT TCAAAGTTTT TTTCTTCCAT
     2101 TTCAGGTGTC GTGAgCTAGC CACtatggtg agcaagggcg aggaggataa catggccatc
     2161 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
     2221 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
     2281 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
     2341 ggctccaagg cctacgtgaa gcaccccgcc gacattcccg actacttgaa gctgtccttc
     2401 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
     2461 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2521 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
     2581 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
     2641 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
     2701 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
     2761 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     2821 gagctgtaca agtctacaga tgtcgaATCC TAACTCGAGC GACGCCTCGA CTGTGCCTTC
     2881 TAGTTGCCAG CCATCTGTTG TTTGCCCCTC CCCCGTGCCT TCCTTGACCC TGGAAGGTGC
     2941 CACTCCCACT GTCCTTTCCT AATAAAATGA GGAAATTGCA TCGCATTGTC TGAGTAGGTG
     3001 TCATTCTATT CTGGGGGGTG GGGTGGGGCA GGACAGCAAG GGGGAGGATT GGGAAGACAA
     3061 TAGCAGGCAT GCTGGGGATG CGGTGGGCTC TATgggctgg aaatctgctc gtcagtggtg
     3121 ctcacactga cgaatcatgt acagatcata ccgatgactg cctggcgact cacaactaag
     3181 caagacagcc ggaaccagcg ccggcgaaca ccactgcata tatggcatat cacaacagtc
     3241 caagcagtta cagagatgtt acgaaccctg aacaggttga ctattaattg tgtctgcttg
     3301 atgtggacac caggtggcgc tggacatcag atttggagag gcagttgtct agggaaccgg
     3361 gctctgtgcc agcgcaggag gcaggctggc tctcctattc cagggatgct catccaggaa
     3421 ggaaaggttg catgctggac acactaacct tacaGCGGTG GGCTCTATGG GGTACCcacg
     3481 catggtatgg atgaacacta gttaaaagtt ttgttacttt atagaagaaa ttttgagttt
     3541 ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt tattattagt
     3601 atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata aacctcgata
     3661 tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac gtacgtcaca
     3721 atatgattat ctttctaggg ttaatctagt atacgcgtat gcggccgctt aattaatcca
     3781 gcttttgttc cctttagtga gggttaattC CGAgcggccg cgtgttacaa ccaattaacc
     3841 aattctgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     3901 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     3961 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     4021 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     4081 gtgacgactg aatccggtga gaatggcaaa agcttatgca tttctttcca gacttgttca
     4141 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     4201 cgtgattgcg cctgagcgag gcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     4261 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     4321 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     4381 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     4441 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     4501 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     4561 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     4621 aatcgcggcc tggagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     4681 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     4741 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     4801 ttgaaggatc agtcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4861 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4921 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4981 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     5041 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     5101 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5161 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5221 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5281 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5341 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5401 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5461 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5521 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtgcgg
     5581 ccgccaatta atacgactca ctatagggcg aattggggcg cgccattcta gattaaccct
     5641 agaaagatag tctgcgtaaa attgacgcat gcattcttga aatattgctc tctctttcta
     5701 aatagcgcga atccgtcgct gtgcatttag gacatctcag tcgccgcttg gagctcccgt
     5761 gaggcgtgct tgtcaatgcg gtaagtgtca ctgattttga actataacga ccgcgtgagt
     5821 caaaatgacg catgattatc ttttacgtga cttttaagat ttaactcata cgataattat
     5881 attgttattt catgttctac ttacgtgata acttattata tatatatttt cttgttatag
     5941 atatcaacta gaatgctagc atgggcccag atctccatgg gCCCTgaatt cgcatctaga
//
