LOCUS       pTBL2620_MTK3_Te        2298 bp    DNA        circular     09-AUG-2024
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    606..609
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1243..1245)
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    599..604
                     /locus_tag="BsaI(2)"
                     /label="BsaI(2)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1247..1252)
                     /locus_tag="BsaI(3)"
                     /label="BsaI(3)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(join(2129..2298,1..594))
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2024..2128)
                     /locus_tag="CamR Promoter"
                     /label="CamR Promoter"
                     /ApEinfo_label="CamR Promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1364..2023)
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_label="CamR"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1255..1363)
                     /locus_tag="CamR Terminator"
                     /label="CamR Terminator"
                     /ApEinfo_label="CamR Terminator"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    610..1242
                     /locus_tag="TetR"
                     /label="TetR"
                     /ApEinfo_label="TetR"
                     /ApEinfo_fwdcolor="#ffcc77"
                     /ApEinfo_revcolor="#ffcc77"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
       61 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      601 tctcatatgt ctcggttgga caaatctaaa gtaatcaact ctgcactgga attgctgaac
      661 gaggtaggca tagagggcct cacaacgagg aagctggccc aaaagctggg cgtcgaacag
      721 ccaaccctgt actggcacgt caagaataaa agggctctcc tggacgcgct ggcaattgag
      781 atgctcgaca gacaccatac acacttttgc ccccttgaag gggaatcctg gcaggacttc
      841 ctgcgaaaca atgccaagtc atttagatgc gctcttctgt ctcatcggga cggtgctaag
      901 gtgcatctgg gtacaagacc cacggaaaag cagtatgaga cactggaaaa tcaactggcc
      961 tttttgtgtc agcagggctt ctctctcgaa aacgcgcttt acgcgctgtc agccgtgggt
     1021 cattttaccc tgggctgcgt gctggaggac caggagcatc aagtggctaa ggaggaacgg
     1081 gaaaccccta ccaccgactc tatgccacct ctcttgcggc aggcaattga gttgttcgac
     1141 caccagggtg ccgagccggc cttcctgttc ggcttggagc ttatcatctg cggcctggag
     1201 aagcagctga agtgtgagag tggaagtcgt acgggaagcg gatcctgaga ccagaccaat
     1261 aaaaaacgcc cggcggcaac cgagcgttct gaacaaatcc agatggagtt ctgaggtcat
     1321 tactggatct atcaacagga gtccaagcga gctcgatatc aaattacgcc ccgccctgcc
     1381 actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa
     1441 cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt
     1501 tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac
     1561 tggtgaaact cacccaggga ttggctgaaa cgaaaaacat attctcaata aaccctttag
     1621 ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact
     1681 gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga
     1741 aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca
     1801 tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa
     1861 acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct
     1921 ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt
     1981 gggatatatc aacggtggta tatccagtga tttttttctc cattttagct tccttagctc
     2041 ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa
     2101 agttggaacc tcttacgtgc ccgatcaatc atgaccaaaa tcccttaacg tgagttttcg
     2161 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2221 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2281 ccggatcaag agctacca    
//
