LOCUS       pTBL2621_MTK3_La        2787 bp    DNA        circular     11-AUG-2024
DEFINITION  .
ACCESSION   <unknown id>                                                       
VERSION     <unknown id>                                                       
COMMENT     
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    606..609
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1732..1734)
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    599..604
                     /locus_tag="BsaI(2)"
                     /label="BsaI(2)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1736..1741)
                     /locus_tag="BsaI(3)"
                     /label="BsaI(3)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(join(2618..2787,1..594))
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(2513..2617)
                     /locus_tag="CamR Promoter"
                     /label="CamR Promoter"
                     /ApEinfo_label="CamR Promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1853..2512)
                     /locus_tag="CamR"
                     /label="CamR"
                     /ApEinfo_label="CamR"
                     /ApEinfo_fwdcolor="#0000ff"
                     /ApEinfo_revcolor="#0000ff"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(1744..1852)
                     /locus_tag="CamR Terminator"
                     /label="CamR Terminator"
                     /ApEinfo_label="CamR Terminator"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1731..1731
                     /locus_tag="TetR"
                     /label="TetR"
                     /ApEinfo_label="TetR"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             1702..1722
                     /codon_start="1"
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#ff0d13"
                     /ApEinfo_revcolor="yellow"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    610..1730
                     /locus_tag="LacI"
                     /label="LacI"
                     /ApEinfo_label="LacI"
                     /ApEinfo_fwdcolor="#ffe51d"
                     /ApEinfo_revcolor="#ffe51d"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
ORIGIN
        1 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
       61 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      121 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      181 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
      241 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
      301 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
      361 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
      421 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
      481 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
      541 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtagtcgg
      601 tctcatatga aaccagtaac attgtatgat gtcgcagagt atgccggtgt ctcttatcag
      661 actgtttcca gagtggtgaa ccaggccagc catgtttctg ccaaaaccag ggaaaaagtg
      721 gaagcagcca tggcagagct gaattacatt cccaacagag tggcacaaca actggcaggc
      781 aaacagagct tgctgattgg agttgccacc tccagtctgg ccctgcatgc accatctcaa
      841 attgtggcag ccattaaatc tagagctgat caactgggag cctctgtggt ggtgtcaatg
      901 gtagaaagaa gtggagttga agcctgtaaa gctgctgtgc acaatcttct ggcacaaaga
      961 gtcagtgggc tgatcattaa ctatccactg gatgaccagg atgccattgc tgtggaagct
     1021 gcctgcacta atgttccagc actctttctt gatgtctctg accagacacc catcaacagt
     1081 attattttct cccatgaaga tggtacaaga ctgggtgtgg agcatctggt tgcattggga
     1141 caccagcaaa ttgcactgct tgcgggccca ctcagttctg tctcagcaag gctgagactg
     1201 gctggctggc ataaatatct cactaggaat caaattcagc caatagctga aagagaaggg
     1261 gactggagtg ccatgtctgg gtttcaacaa accatgcaaa tgctgaatga gggcattgtt
     1321 cccactgcaa tgctggttgc caatgatcag atggcactgg gtgcaatgag agccattact
     1381 gagtctgggc tgagagttgg tgcagatatc tcggtagtgg gatacgacga taccgaagac
     1441 agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa
     1501 accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg
     1561 ttgccagtct cactggtgaa gagaaaaacc accctggcac ccaatacaca aactgcctct
     1621 ccccgggcat tggctgattc actcatgcag ctagcaagac aggtttccag actggaaagt
     1681 gggcagagca gcctgaggcc tcctaagaag aagaggaagg ttggctctgg atcctgagac
     1741 cagaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca gatggagttc
     1801 tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca aattacgccc
     1861 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1921 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1981 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     2041 aatcaaaact ggtgaaactc acccagggat tggctgaaac gaaaaacata ttctcaataa
     2101 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     2161 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     2221 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     2281 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     2341 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     2401 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     2461 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     2521 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     2581 tatggtgaaa gttggaacct cttacgtgcc cgatcaatca tgaccaaaat cccttaacgt
     2641 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2701 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2761 gtttgtttgc cggatcaaga gctacca   
//
