LOCUS       pTBL3342_MTK_PB_       14794 bp    DNA        circular     08-SEP-2024
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     Golden Gate reaction:
COMMENT     pTBL3337 MTK_TU-cHS4-pCAG-PEMax-BGHpA from 169 (Esp3I) to 9108
            (Esp3I) 
COMMENT     pTBL3339 MTK_TU-pUBC-puro-beta_glob from 169 (Esp3I) to 2971
            (Esp3I) 
COMMENT     pTBL2367 MTK0-PB-nohyg from 1339 (Esp3I) to 14 (Esp3I) 
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     regulatory      926..1213
                     /locus_tag="CAG_enhancer"
                     /label="CAG_enhancer"
                     /ApEinfo_label="CAG_enhancer"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    9234..9237
                     /locus_tag="BsmBI"
                     /label="BsmBI"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    2585..2588
                     /locus_tag="BsaI"
                     /label="BsaI"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        9500..10716
                     /locus_tag="hUbC_promoter"
                     /label="hUbC_promoter"
                     /ApEinfo_label="hUbC_promoter"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1..25
                     /locus_tag="ConS scar"
                     /label="ConS scar"
                     /ApEinfo_label="ConS scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    join(323..570,854..885)
                     /locus_tag="HS4"
                     /label="HS4"
                     /ApEinfo_label="HS4"
                     /ApEinfo_fwdcolor="#f58a5e"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer          2533..2552
                     /locus_tag="pCAG_F_primer"
                     /label="pCAG_F_primer"
                     /ApEinfo_label="pCAG_F_primer"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    905..2579
                     /locus_tag="CAG promotor"
                     /label="CAG promotor"
                     /ApEinfo_label="CAG promotor"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    1483..1502
                     /locus_tag="sgRNA"
                     /label="sgRNA"
                     /ApEinfo_label="sgRNA"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    1..4
                     /locus_tag="BsmBI(1)"
                     /label="BsmBI(1)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9259..9495
                     /locus_tag="E4 Insulator"
                     /label="E4 Insulator"
                     /ApEinfo_label="E4 Insulator"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        9965..9980
                     /locus_tag="hUbC_promoter(1)"
                     /label="hUbC_promoter(1)"
                     /ApEinfo_label="hUbC_promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    40..319
                     /locus_tag="HS4(1)"
                     /label="HS4(1)"
                     /ApEinfo_label="HS4"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2589..2604
                     /Sequence="AGCCGCCACCAUGAAACGGACAGCCGACG"
                     /%GC="65.5"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0198"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:327-97kkp7h">PC0198</a>"
                     /locus_tag="PC0198"
                     /label="PC0198"
                     /ApEinfo_label="PC0198"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2643..6743
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from
                     theStreptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA
                     dueto the H840A mutation in the HNH catalytic domain"
                     /Original_Translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLG
                     NTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVD
                     DSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
                     LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKA
                     ILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSK
                     DTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYD
                     EHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMD
                     GTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEK
                     ILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDK
                     NLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRK
                     VTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDIL
                     EDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQ
                     SGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPA
                     IKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKE
                     LGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLK
                     DDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERG
                     GLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVS
                     DFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMI
                     AKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDF
                     ATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPT
                     VAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLII
                     KLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQ
                     KQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
                     FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /modified_by="chenp"
                     /locus_tag="Cas9(R221K N394K H840A)"
                     /label="Cas9(R221K N394K H840A)"
                     /ApEinfo_label="Cas9(R221K N394K H840A)"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2658..2675
                     /Sequence="ATCGGCCTGGACAUCGGC"
                     /%GC="66.7"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0293"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1t-9atlcx8">PC0293</a>"
                     /locus_tag="PC0293"
                     /label="PC0293"
                     /ApEinfo_label="PC0293"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3024..3043)
                     /Sequence="ATGGTGGGGTACUTCTCGTG"
                     /%GC="55.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0229"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1wd-998axgd">PC0229</a>"
                     /locus_tag="PC0229"
                     /label="PC0229"
                     /ApEinfo_label="PC0229"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3123..3142)
                     /Sequence="AAGTGGCCCCGGAACTTGAT"
                     /Hairpin_Tm="33.0"
                     /Tm="61.8"
                     /Self_Dimer_Tm="0.7"
                     /%GC="55.0"
                     /created_by="primer3"
                     /locus_tag="PC0873"
                     /label="PC0873"
                     /ApEinfo_label="PC0873"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3273..3304)
                     /Sequence="AGCTTTCTGCTCTTGCTCAGTCTGGCAGACAG"
                     /Tm="71.1"
                     /%GC="53.1"
                     /Hairpin_Tm="52.7"
                     /Self_Dimer_Tm="40.1"
                     /created_by="chenp"
                     /locus_tag="PC1651"
                     /label="PC1651"
                     /ApEinfo_label="PC1651"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3300..3301
                     /Original_Bases="CG"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="CG"
                     /label="CG"
                     /ApEinfo_label="CG"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3807..3836)
                     /Sequence="CAGCAGGTCCTCTCTCTTCAGCTTCACGAG"
                     /Tm="69.4"
                     /%GC="56.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /locus_tag="PC1659"
                     /label="PC1659"
                     /ApEinfo_label="PC1659"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    3821..3821
                     /Original_Bases="C"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="C"
                     /label="C"
                     /ApEinfo_label="C"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4678..4702
                     /Sequence="TGGATTTCCTGAAGTCCGACGGCTT"
                     /%GC="52.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ682"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2a-9qpay4z">KZ682</a>"
                     /locus_tag="KZ682"
                     /label="KZ682"
                     /ApEinfo_label="KZ682"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5149..5178
                     /Sequence="ATGTGGACGCUATCGTGCCTCAGAGCTTTC"
                     /%GC="53.3"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0892"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:b1-b14udub">PC0892</a>"
                     /locus_tag="PC0892"
                     /label="PC0892"
                     /ApEinfo_label="PC0892"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5942..5961
                     /Sequence="GACAGGCGGCTTCAGCAAAG"
                     /%GC="60.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1504"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2c-cej84ut">PC1504</a>"
                     /locus_tag="PC1504"
                     /label="PC1504"
                     /ApEinfo_label="PC1504"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(6509..6536)
                     /Sequence="ATCCCGGTGCTTGUTGTAGGCGGACAGC"
                     /%GC="60.7"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1653"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:l3-d24gkh0">PC1653</a>"
                     /locus_tag="PC1653"
                     /label="PC1653"
                     /ApEinfo_label="PC1653"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(6714..6740)
                     /Sequence="ACCTCCCAGCUGAGACAGGTCGATCCG"
                     /%GC="63.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1494"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:y-cej84u0">PC1494</a>"
                     /locus_tag="PC1494"
                     /label="PC1494"
                     /ApEinfo_label="PC1494"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6846..8876
                     /created_by="jessiedavis"
                     /locus_tag="RT codon optimized Genscript"
                     /label="RT codon optimized Genscript"
                     /ApEinfo_label="RT codon optimized Genscript"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8919..8921
                     /Original_Bases="SV40 NLS"
                     /note="Geneious type: Editing History Insertion"
                     /locus_tag="SV40 NLS"
                     /label="SV40 NLS"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             2589..2642
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation=MKRTADGSEFESPKKKRKV
                     /locus_tag="SV40 NLS(1)"
                     /label="SV40 NLS(1)"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2643..2663
                     /Sequence="GACAAGAAGTACAGCATCGGC"
                     /%GC="52.4"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1498"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1i-cej84ud">PC1498</a>"
                     /locus_tag="PC1498"
                     /label="PC1498"
                     /ApEinfo_label="PC1498"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2678..2702
                     /Sequence="CAACTCTGTGGGCTGGGCCGTGATC"
                     /%GC="64.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ678"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1q-9qpay45">KZ678</a>"
                     /locus_tag="KZ678"
                     /label="KZ678"
                     /ApEinfo_label="KZ678"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3031..3050
                     /Sequence="AGTACCCCACCAUCTACCAC"
                     /%GC="55.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0228"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1w8-998axgb">PC0228</a>"
                     /locus_tag="PC0228"
                     /label="PC0228"
                     /ApEinfo_label="PC0228"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3123..3142)
                     /Sequence="AAGTGGCCCCGGAACTTGAT"
                     /%GC="55.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0873"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:8e-b14udti">PC0873</a>"
                     /locus_tag="PC0873(1)"
                     /label="PC0873(1)"
                     /ApEinfo_label="PC0873"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     USER            3292..3304
                     /created_by="chenp"
                     /locus_tag="USER"
                     /label="USER"
                     /ApEinfo_label="USER"
                     /ApEinfo_fwdcolor="#00DF87"
                     /ApEinfo_revcolor="#00DF87"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3678..3702
                     /Sequence="TACAAAGAGATTTTCTTCGACCAGA"
                     /%GC="36.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ680"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:20-9qpay4h">KZ680</a>"
                     /locus_tag="KZ680"
                     /label="KZ680"
                     /ApEinfo_label="KZ680"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc            3819..3821
                     /created_by="chenp"
                     /locus_tag="N394K"
                     /label="N394K"
                     /ApEinfo_label="N394K"
                     /ApEinfo_fwdcolor="#008065"
                     /ApEinfo_revcolor="#008065"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3873..3891
                     /Sequence="CACCAGATCCACCTGGGAG"
                     /%GC="63.2"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1501"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1x-cej84uk">PC1501</a>"
                     /locus_tag="PC1501"
                     /label="PC1501"
                     /ApEinfo_label="PC1501"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5136..5180
                     /Sequence="CTGTCCGACTACGATGTGGACGCTATCGTGCCTCAGAGCTTTCTG"
                     /created_by="Alexander Sousa"
                     /locus_tag="AVA216"
                     /label="AVA216"
                     /ApEinfo_label="AVA216"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5178..5202
                     /Sequence="CTGAAGGACGACTCCATCGACAACA"
                     /%GC="52.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ683"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2f-9qpay56">KZ683</a>"
                     /locus_tag="KZ683"
                     /label="KZ683"
                     /ApEinfo_label="KZ683"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(6016..6040)
                     /Sequence="AAGCCGCCGUACTTCTTAGGGTCCC"
                     /%GC="60.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0895"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:bg-b14uduh">PC0895</a>"
                     /locus_tag="PC0895"
                     /label="PC0895"
                     /ApEinfo_label="PC0895"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     6523..6551
                     /Sequence="ACAAGCACCGGGAUAAGCCCATCAGAGAG"
                     /%GC="55.2"
                     /note="Derived using Geneious Prime 2021.0.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1652"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:ky-d24gkgz">PC1652</a>"
                     /locus_tag="PC1652"
                     /label="PC1652"
                     /ApEinfo_label="PC1652"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(6715..6733)
                     /Sequence="AGCTGAGACAGGUCGATCC"
                     /%GC="57.9"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0233"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1wx-998axgp">PC0233</a>"
                     /locus_tag="PC0233"
                     /label="PC0233"
                     /ApEinfo_label="PC0233"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     7265..7284
                     /Sequence="CCCTTCTCACCAGTGGTATA"
                     /%GC="50.0"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="JRDSeq19"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:16w-cltirny">JRDSeq19</a>"
                     /locus_tag="JRDSeq19"
                     /label="JRDSeq19"
                     /ApEinfo_label="JRDSeq19"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8943..8980
                     /Original_Bases="Geneious type: Editing History Insertion"
                     /note="Geneious type: Editing History Insertion"
                     /locus_tag="Geneious type: Editing History Insertion"
                     /label="Geneious type: Editing History Insertion"
                     /ApEinfo_label="Geneious type: Editing History Insertion"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2602..2634
                     /Sequence="ACGGAAGCGAGUTCGAGTCACCAAAGAAGAAGC"
                     /%GC="51.5"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0738"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1ci-a43x1p9">PC0738</a>"
                     /locus_tag="PC0738"
                     /label="PC0738"
                     /ApEinfo_label="PC0738"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2650..2671)
                     /Sequence="ATGTCCAGGCCGAUGCTGTACT"
                     /%GC="54.5"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0292"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1o-9atlcx0">PC0292</a>"
                     /locus_tag="PC0292"
                     /label="PC0292"
                     /ApEinfo_label="PC0292"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2721..2742)
                     /Sequence="GCACCTTGAATTTCTTGCTGGG"
                     /%GC="50.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1499"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1n-cej84uf">PC1499</a>"
                     /locus_tag="PC1499"
                     /label="PC1499"
                     /ApEinfo_label="PC1499"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3060..3079
                     /Sequence="AAACTGGTGGACAGCACCGA"
                     /Hairpin_Tm="51.8"
                     /Tm="62.0"
                     /Self_Dimer_Tm="None"
                     /%GC="55.0"
                     /created_by="primer3"
                     /locus_tag="PC0872"
                     /label="PC0872"
                     /ApEinfo_label="PC0872"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3177..3197
                     /Sequence="GACGTGGACAAGCTGTTCATC"
                     /%GC="52.4"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1500"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1s-cej84uh">PC1500</a>"
                     /locus_tag="PC1500"
                     /label="PC1500"
                     /ApEinfo_label="PC1500"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3292..3324
                     /Sequence="AGAGCAGAAAGCTGGAAAATCTGATCGCCCAGC"
                     /Tm="71.5"
                     /%GC="51.5"
                     /Hairpin_Tm="58.2"
                     /Self_Dimer_Tm="29.7"
                     /created_by="chenp"
                     /locus_tag="PC1650"
                     /label="PC1650"
                     /ApEinfo_label="PC1650"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(3800..3832)
                     /Sequence="AGGTCCTCTCTCTTCAGCTTCACGAGCAGTTCC"
                     /Tm="71.4"
                     /%GC="54.5"
                     /Hairpin_Tm="54.3"
                     /Self_Dimer_Tm="0.5"
                     /created_by="chenp"
                     /locus_tag="PC1649"
                     /label="PC1649"
                     /ApEinfo_label="PC1649"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     USER            3820..3832
                     /created_by="chenp"
                     /locus_tag="USER(1)"
                     /label="USER(1)"
                     /ApEinfo_label="USER"
                     /ApEinfo_fwdcolor="#13AAFE"
                     /ApEinfo_revcolor="#13AAFE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4178..4202
                     /Sequence="GCTGTACGAGTACTTCACCGTGTAT"
                     /%GC="48.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ681"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:25-9qpay4m">KZ681</a>"
                     /locus_tag="KZ681"
                     /label="KZ681"
                     /ApEinfo_label="KZ681"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5136..5180)
                     /Sequence="CAGAAAGCTCTGAGGCACGATAGCGTCCACATCGTAGTCGGACAG"
                     /created_by="Alexander Sousa"
                     /locus_tag="AVA215"
                     /label="AVA215"
                     /ApEinfo_label="AVA215"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5255..5276
                     /Sequence="CGAAGAGGTCGTGAAGAAGATG"
                     /%GC="50.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1503"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:27-cej84us">PC1503</a>"
                     /locus_tag="PC1503"
                     /label="PC1503"
                     /ApEinfo_label="PC1503"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     6178..6202
                     /Sequence="ACTTTCTGGAAGCCAAGGGCTACAA"
                     /%GC="48.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ685"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2p-9qpay5r">KZ685</a>"
                     /locus_tag="KZ685"
                     /label="KZ685"
                     /ApEinfo_label="KZ685"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     6637..6656
                     /Sequence="GGAAGAGGTACACCAGCACC"
                     /%GC="60.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1505"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2h-cej84uv">PC1505</a>"
                     /locus_tag="PC1505"
                     /label="PC1505"
                     /ApEinfo_label="PC1505"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             6744..6845
                     /created_by="chenp"
                     /locus_tag="SGGSx2-sv40NLS-SGGSx2"
                     /label="SGGSx2-sv40NLS-SGGSx2"
                     /ApEinfo_label="SGGSx2-sv40NLS-SGGSx2"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(8590..8608)
                     /Sequence="AAGGCATATCTGGAGTCTG"
                     /%GC="47.4"
                     /note="Derived using Geneious Prime 2020.1.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="JRDSeq18"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:16r-cltirnv">JRDSeq18</a>"
                     /locus_tag="JRDSeq18"
                     /label="JRDSeq18"
                     /ApEinfo_label="JRDSeq18"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             8952..8980
                     /created_by="chenp"
                     /locus_tag="c-Myc NLS"
                     /label="c-Myc NLS"
                     /ApEinfo_label="c-Myc NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2589..2619)
                     /Sequence="ACTCGAACTCGCTUCCGTCGGCTGTCCGTTTCATGGTGGCGGCTCTC
                     "
                     /%GC="61.7"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0234"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1x2-998axgr">PC0234</a>"
                     /locus_tag="PC0234"
                     /label="PC0234"
                     /ApEinfo_label="PC0234"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2612..2641)
                     /Sequence="ACTTTCCGCTUCTTCTTTGGTGACTCGAAC"
                     /%GC="46.7"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0831"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1i4-acth48u">PC0831</a>"
                     /locus_tag="PC0831"
                     /label="PC0831"
                     /ApEinfo_label="PC0831"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     2653..2677
                     /Sequence="ACAGCATCGGCCUGGACATCGGCAC"
                     /%GC="64.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1335"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:93-cczyqbw">PC1335</a>"
                     /locus_tag="PC1335"
                     /label="PC1335"
                     /ApEinfo_label="PC1335"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(2782..2806)
                     /Sequence="CCGCTGTCGAACAGCAGGGCTCCGA"
                     /%GC="68.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ677"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1l-9qpay42">KZ677</a>"
                     /locus_tag="KZ677"
                     /label="KZ677"
                     /ApEinfo_label="KZ677"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3060..3079
                     /Sequence="AAACTGGTGGACAGCACCGA"
                     /%GC="55.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0872"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:89-b14udte">PC0872</a>"
                     /locus_tag="PC0872(1)"
                     /label="PC0872(1)"
                     /ApEinfo_label="PC0872"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3178..3202
                     /Sequence="ACGTGGACAAGCTGTTCATCCAGCT"
                     /%GC="52.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ679"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:1v-9qpay4b">KZ679</a>"
                     /locus_tag="KZ679"
                     /label="KZ679"
                     /ApEinfo_label="KZ679"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc            3300..3302
                     /created_by="chenp"
                     /modified_by="chenp"
                     /locus_tag="R221K"
                     /label="R221K"
                     /ApEinfo_label="R221K"
                     /ApEinfo_fwdcolor="#00D95A"
                     /ApEinfo_revcolor="#00D95A"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3807..3836
                     /Sequence="CTCGTGAAGCTGAAGAGAGAGGACCTGCTG"
                     /Tm="69.4"
                     /%GC="56.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /created_by="chenp"
                     /locus_tag="PC1658"
                     /label="PC1658"
                     /ApEinfo_label="PC1658"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     3820..3845
                     /Sequence="AGAGAGAGGACCTGCTGCGGAAGCAG"
                     /Tm="69.6"
                     /%GC="61.5"
                     /Hairpin_Tm="74.4"
                     /Self_Dimer_Tm="27.9"
                     /created_by="chenp"
                     /locus_tag="PC1648"
                     /label="PC1648"
                     /ApEinfo_label="PC1648"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     4562..4583
                     /Sequence="CCTGTTCGACGACAAAGTGATG"
                     /%GC="50.0"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC1502"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:22-cej84ul">PC1502</a>"
                     /locus_tag="PC1502"
                     /label="PC1502"
                     /ApEinfo_label="PC1502"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(5136..5159)
                     /Sequence="AGCGTCCACAUCGTAGTCGGACAG"
                     /%GC="58.3"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0893"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:b6-b14udud">PC0893</a>"
                     /locus_tag="PC0893"
                     /label="PC0893"
                     /ApEinfo_label="PC0893"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     5678..5702
                     /Sequence="CAAGGTGTACGACGTGCGGAAGATG"
                     /%GC="56.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ684"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2k-9qpay5g">KZ684</a>"
                     /locus_tag="KZ684"
                     /label="KZ684"
                     /ApEinfo_label="KZ684"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(6265..6287)
                     /Sequence="AGAGGCCAGCAUTCTCTTCCGGC"
                     /%GC="60.9"
                     /note="Derived using Geneious Prime 2020.2.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="PC0897"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:bq-b14uduk">PC0897</a>"
                     /locus_tag="PC0897"
                     /label="PC0897"
                     /ApEinfo_label="PC0897"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     6678..6702
                     /Sequence="CTGATCCACCAGAGCATCACCGGCC"
                     /%GC="64.0"
                     /note="Derived using Geneious Prime 2020.2.2 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="KZ686"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href="urn:local:.:2u-9qpay5z">KZ686</a>"
                     /locus_tag="KZ686"
                     /label="KZ686"
                     /ApEinfo_label="KZ686"
                     /ApEinfo_fwdcolor="#14c0bd"
                     /ApEinfo_revcolor="#4ec02b"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    6746..6842
                     /Original_Bases="TGGAGGATCTAGCGGAGGATCCTCTGGCAGCGAGACACCAG
                     GAACAAGCGAGTCAGCAACACCAGAGAGCAGTGGCGGCAGCAGCGGCGGCAGC"
                     /note="Geneious type: Editing History Replacement"
                     /locus_tag="TGGAGGATCTAGCGGAGGATCCTCTGGCAGCGAGACACCAGG
                     AACAAGCG AGTCAGCAACACCAGAGAGCAGTGGCGGCAGCAGCGGCGGCAGC"
                     /label="TGGAGGATCTAGCGGAGGATCCTCTGGCAGCGAGACACCAGG
                     AACAAGCG AGTCAGCAACACCAGAGAGCAGTGGCGGCAGCAGCGGCGGCAGC"
                     /ApEinfo_label="TGGAGGATCTAGCGGAGGATCCTCTGGCAGCGAGACACCAGG
                     AACAAGCG AGTCAGCAACACCAGAGAGCAGTGGCGGCAGCAGCGGCGGCAGC"
                     /ApEinfo_fwdcolor="#7eff74"
                     /ApEinfo_revcolor="#7eff74"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             8889..8942
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /Original_Translation="KRTADGSEFEPKKKRKV"
                     /locus_tag="SV40 NLS(2)"
                     /label="SV40 NLS(2)"
                     /ApEinfo_label="SV40 NLS"
                     /ApEinfo_fwdcolor="#e9d024"
                     /ApEinfo_revcolor="#e9d024"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     USER            8966..8980
                     /created_by="chenp"
                     /locus_tag="USER(2)"
                     /label="USER(2)"
                     /ApEinfo_label="USER"
                     /ApEinfo_fwdcolor="#3A93FE"
                     /ApEinfo_revcolor="#3A93FE"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    8009..8014
                     /locus_tag="Esp3I_site_changed"
                     /label="Esp3I_site_changed"
                     /ApEinfo_label="Esp3I_site_changed"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     Terminator      8994..9229
                     /locus_tag="BGH-PA"
                     /label="BGH-PA"
                     /ApEinfo_label="BGH-PA"
                     /ApEinfo_fwdcolor="#c7b0e3"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9230..9233
                     /locus_tag="BsaI(1)"
                     /label="BsaI(1)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        9981..9996
                     /locus_tag="hUbC_promoter(2)"
                     /label="hUbC_promoter(2)"
                     /ApEinfo_label="hUbC_promoter"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9903..10714
                     /locus_tag="intron"
                     /label="intron"
                     /ApEinfo_label="intron"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9234..9258
                     /locus_tag="Con5 scar"
                     /label="Con5 scar"
                     /ApEinfo_label="Con5 scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9810..9814
                     /locus_tag="TATA box"
                     /label="TATA box"
                     /ApEinfo_label="TATA box"
                     /ApEinfo_fwdcolor="#ff2c78"
                     /ApEinfo_revcolor="#ff2c78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    9839..9839
                     /locus_tag="first transcribed nt"
                     /label="first transcribed nt"
                     /ApEinfo_label="first transcribed nt"
                     /ApEinfo_fwdcolor="#ff2c78"
                     /ApEinfo_revcolor="#ff2c78"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    10722..10725
                     /locus_tag="BsaI(2)"
                     /label="BsaI(2)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    10726..11318
                     /locus_tag="puro"
                     /label="puro"
                     /ApEinfo_label="puro"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
     misc_feature    11109..11114
                     /locus_tag="Esp3Isite"
                     /label="Esp3Isite"
                     /ApEinfo_label="Esp3Isite"
                     /ApEinfo_fwdcolor="#009091"
                     /ApEinfo_revcolor="#009091"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    11203..11208
                     /locus_tag="BsaIsite"
                     /label="BsaIsite"
                     /ApEinfo_label="BsaIsite"
                     /ApEinfo_fwdcolor="#009091"
                     /ApEinfo_revcolor="#009091"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      11333..11848
                     /locus_tag="rb_glob_PA_terminator"
                     /label="rb_glob_PA_terminator"
                     /ApEinfo_label="rb_glob_PA_terminator"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    11890..11893
                     /locus_tag="BsaI(3)"
                     /label="BsaI(3)"
                     /ApEinfo_label="BsaI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    11894..12036
                     /locus_tag="ConE'"
                     /label="ConE'"
                     /ApEinfo_label="ConE'"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12062..12244
                     /locus_tag="Insulator A1"
                     /label="Insulator A1"
                     /ApEinfo_label="Insulator A1"
                     /ApEinfo_fwdcolor="#b4abac"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12491..12521
                     /locus_tag="31bp spacer"
                     /label="31bp spacer"
                     /ApEinfo_label="31bp spacer"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    14447..14465
                     /locus_tag="19bp IR"
                     /label="19bp IR"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#9eafd2"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    14615..14620
                     /locus_tag="CAAAT repeat"
                     /label="CAAAT repeat"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#75c6a9"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12522..12534
                     /locus_tag="13bp TR"
                     /label="13bp TR"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#d59687"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     14383..14402
                     /locus_tag="T7 Forward (Elim)"
                     /label="T7 Forward (Elim)"
                     /ApEinfo_label="T7 Forward (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12037..12061
                     /locus_tag="ConE scar"
                     /label="ConE scar"
                     /ApEinfo_label="ConE scar"
                     /ApEinfo_fwdcolor="#ffef86"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12472..12490
                     /locus_tag="19bp IR(1)"
                     /label="19bp IR(1)"
                     /ApEinfo_label="19bp IR"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     primer_bind     complement(12584..12603)
                     /locus_tag="T3 (Elim)"
                     /label="T3 (Elim)"
                     /ApEinfo_label="T3 (Elim)"
                     /ApEinfo_fwdcolor="#85dae9"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        12297..12544
                     /locus_tag="3' PB repeat"
                     /label="3' PB repeat"
                     /ApEinfo_label="3' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(12324..12329)
                     /locus_tag="CAAAAT repeat"
                     /label="CAAAAT repeat"
                     /ApEinfo_label="CAAAAT repeat"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     terminator      complement(12616..12642)
                     /locus_tag="KanR Terminator"
                     /label="KanR Terminator"
                     /ApEinfo_label="KanR Terminator"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(12585..12603)
                     /locus_tag="T3 promoter"
                     /label="T3 promoter"
                     /ApEinfo_label="T3 promoter"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     rep_origin      complement(13607..14370)
                     /locus_tag="ColE1"
                     /label="ColE1"
                     /ApEinfo_label="ColE1"
                     /ApEinfo_fwdcolor="#7f7f7f"
                     /ApEinfo_revcolor="#7f7f7f"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        14427..14748
                     /locus_tag="5' PB repeat"
                     /label="5' PB repeat"
                     /ApEinfo_label="5' PB repeat"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     protein_bind    12037..12040
                     /locus_tag="BsmBI(2)"
                     /label="BsmBI(2)"
                     /ApEinfo_label="BsmBI"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    14431..14443
                     /locus_tag="13bp TR(1)"
                     /label="13bp TR(1)"
                     /ApEinfo_label="13bp TR"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    complement(14588..14593)
                     /locus_tag="CAAAT repeat(1)"
                     /label="CAAAT repeat(1)"
                     /ApEinfo_label="CAAAT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             complement(12643..13458)
                     /locus_tag="KanR"
                     /label="KanR"
                     /ApEinfo_label="KanR"
                     /ApEinfo_fwdcolor="#008000"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    14705..14711
                     /locus_tag="ACTTATT repeat"
                     /label="ACTTATT repeat"
                     /ApEinfo_label="ACTTATT repeat"
                     /ApEinfo_fwdcolor="#d6b295"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     CDS             12271..12290
                     /locus_tag="sfGFP"
                     /label="sfGFP"
                     /ApEinfo_label="sfGFP"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     promoter        complement(13459..13606)
                     /locus_tag="KanR Promoter"
                     /label="KanR Promoter"
                     /ApEinfo_label="KanR Promoter"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12265..12270
                     /locus_tag="KpnI"
                     /label="KpnI"
                     /ApEinfo_label="KpnI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    12291..12296
                     /locus_tag="SpeI"
                     /label="SpeI"
                     /ApEinfo_label="SpeI"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="cyan"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {} 0} width 5 offset 0"
     misc_feature    572..853
                     /locus_tag="HS4(2)"
                     /label="HS4(2)"
                     /ApEinfo_label="HS4"
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 0.5 0 1 2 0 0 -1 0
                     -0.5} {0 .5 .1 .5 .1 -.5 0 -.5} 0} width 5 offset 0"
ORIGIN
        1 ctgaactggc cgataattgc agacgATCGA ATTCGAGTTG GCGCGCCTGT CATTCTAAAT
       61 CTCTCTTTCA GCCTAAAGCT TTTTCCCCGT ATCCCCCCAG GTGTCTGCAG GCTCAAAGAG
      121 CAGCGAGAAG CGTTCAGAGG AAAGCGATCC CGTGCCACCT TCCCCGTGCC CGGGCTGTCC
      181 CCGCACGCTG CCGGCTCGGG GATGCGGGGG GAGCGCCGGA CCGGAGCGGA GCCCCGGGCG
      241 GCTCGCTGCT GCCCCCTAGC GGGGGAGGGA CGTAATTACA TCCCTGGGGG CTTTGGGAGG
      301 GGGCTGTCCC CGTGAGCTCC CAGGCGCGCC TGTCATTCTA AATCTCTCTT TCAGCCTAAA
      361 GCTTTTTCCC CGTATCCCCC CAGGTGTCTG CAGGCTCAAA GAGCAGCGAG AAGCGTTCAG
      421 AGGAAAGCGA TCCCGTGCCA CCTTCCCCGT GCCCGGGCTG TCCCCGCACG CTGCCGGCTC
      481 GGGGATGCGG GGGGAGCGCC GGACCGGAGC GGAGCCCCGG GCGGCTCGCT GCTGCCCCCT
      541 AGCGGGGGAG GGACGTAATT ACATCCCTGG gggctttggg agggggctgt ccccgtgagc
      601 tcccaggcgc gcctgtcatt ctaaatctct ctttcagcct aaagcttttt ccccgtatcc
      661 ccccaggtgt ctgcaggctc aaagagcagc gagaagcgtt cagaggaaag cgatcccgtg
      721 ccaccttccc cgtgcccggg ctgtccccgc acgctgccgg ctcggggatg cggggggagc
      781 gccggaccgg agcggagccc cgggcggctc gctgctgccc cctagcgggg gagggacgta
      841 attacatccc tggGGGCTTT GGGGGGGGGC TGTCCCCGTG AGCTCAATAA AAGAGCCCAC
      901 AACGCCATAT ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC
      961 GCCCAACGAC CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT
     1021 AGGGACTTTC CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT
     1081 ACATCAAGTG TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC
     1141 CGCCTGGCAT TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA
     1201 CGTATTAGTC ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC
     1261 CATCTCCCCC CCCCTCCCCA CCCCCAATTT TGTATTTATT TATTTTTTAA TTATTTTGTG
     1321 CAGCGATGGG GGCGGGGGGG GGGGGGGGGG GGCGCGCGCC AGGCGGGGCG GGGCGGGGCG
     1381 AGGGGCGGGG CGGGGCGAGG CGGAGAGGTG CGGCGGCAGC CAATCAGAGC GGCGCGCTCC
     1441 GAAAGTTTCC TTTTATGGCG AGGCGGCGGC GGCGGCGGCC CTATAAAAAG CGAAGCGCGC
     1501 GGCGGGCGGG AGTCGCTGCG CGCTGCCTTC GCCCCGTGCC CCGCTCCGCC GCCGCCTCGC
     1561 GCCGCCCGCC CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC
     1621 CTTCTCCTCC GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTTGTTTCT TTTCTGTGGC
     1681 TGCGTGAAAG CCTTGAGGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGAGC GGCTCGGGGG
     1741 GTGCGTGCGT GTGTGTGTGC GTGGGGAGCG CCGCGTGCGG CTCCGCGCTG CCCGGCGGCT
     1801 GTGAGCGCTG CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CAGTGTGCGC GAGGGGAGCG
     1861 CGGCCGGGGG CGGTGCCCCG CGGTGCGGGG GGGGCTGCGA GGGGAACAAA GGCTGCGTGC
     1921 GGGGTGTGTG CGTGGGGGGG TGAGCAGGGG GTGTGGGCGC GTCGGTCGGG CTGCAACCCC
     1981 CCCTGCACCC CCCTCCCCGA GTTGCTGAGC ACGGCCCGGC TTCGGGTGCG GGGCTCCGTa
     2041 CGGGGCGTGG CGCGGGGCTC GCCGTGCCGG GCGGGGGGTG GCGGCAGGTG GGGGTGCCGG
     2101 GCGGGGCGGG GCCGCCTCGG GCCGGGGAGG GCTCGGGGGA GGGGCGCGGC GGcCCCCGGA
     2161 GTGCCGGCGG CTGTCGAGGC GCGGCGAGCC GCAGCCATTG CCTTTTATGG TAATCGTGCG
     2221 AGAGGGCGCA GGGACTTCCT TTGTCCCAAA TCTGTGCGGA GCCGAAATCT GGGAGGCGCC
     2281 GCCGCACCCC CTCTAGCGGG CGCgGGGCGA AGCGGTGCGG CGCCGGCAGG AAGGAAATGG
     2341 GCGGGGAGGG CCTTCGTGCG TCGCCGCGCC GCCGTCCCCT TCTCCcTCTC CAGCCTCGGG
     2401 GCTGTCCGCg GGGGGACGGC TGCCTTCGGG GGGGACGGGG CAGGGCGGGG TTCGGCTTCT
     2461 GGCGTGTGAC CGGCGGCTCT AGAGCCTCTG CTAACCATGT TCATGCCTTC TTCTTTTTCC
     2521 TACAGCTCCT GGGCAACGTG CTGGTTATTG TGCTGTCTCA TCATTTTGGC AAAGaattcg
     2581 ccactatgaa acggacagcc gacggaagcg agttcgagtc accaaagaag aagcggaaag
     2641 tcgacaagaa gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga
     2701 tcaccgacga gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc
     2761 acagcatcaa gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg
     2821 ccacccggct gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct
     2881 atctgcaaga gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac
     2941 tggaagagtc cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca
     3001 acatcgtgga cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga
     3061 aactggtgga cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca
     3121 tgatcaagtt ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg
     3181 tggacaagct gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca
     3241 tcaacgccag cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa
     3301 agctggaaaa tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc
     3361 tgattgccct gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg
     3421 atgccaaact gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc
     3481 agatcggcga ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc
     3541 tgctgagcga catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta
     3601 tgatcaagag atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc
     3661 agcagctgcc tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg
     3721 gctacattga cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg
     3781 aaaagatgga cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga
     3841 agcagcggac cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg
     3901 ccattctgcg gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg
     3961 agaagatcct gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca
     4021 gattcgcctg gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag
     4081 tggtggacaa gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga
     4141 acctgcccaa cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt
     4201 ataacgagct gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga
     4261 gcggcgagca gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg
     4321 tgaagcagct gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct
     4381 ccggcgtgga agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta
     4441 tcaaggacaa ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc
     4501 tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc
     4561 acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca
     4621 ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg
     4681 atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca
     4741 gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc
     4801 acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag
     4861 tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga
     4921 tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa
     4981 tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg
     5041 tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg
     5101 atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat gtggacgcta
     5161 tcgtgcctca gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg
     5221 acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga
     5281 actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga
     5341 ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc
     5401 tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca
     5461 ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca
     5521 agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact
     5581 accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg atcaaaaagt
     5641 accctaagct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga
     5701 tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca
     5761 acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc
     5821 ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg
     5881 ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgaggtgc
     5941 agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat aagctgatcg
     6001 ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc accgtggcct
     6061 attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg aagagtgtga
     6121 aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat cccatcgact
     6181 ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag ctgcctaagt
     6241 actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc ggcgaactgc
     6301 agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac ctggccagcc
     6361 actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg tttgtggaac
     6421 agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc aagagagtga
     6481 tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac cgggataagc
     6541 ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat ctgggagccc
     6601 ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc agcaccaaag
     6661 aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag acacggatcg
     6721 acctgtctca gctgggaggt gactccggcg gaagctctgg tggcagcaag cggaccgccg
     6781 acggctctga attcgagagc cctaagaaga aaagaaaggt gagcggaggc tctagcggcg
     6841 gaagcaccct gaacattgaa gacgagtata gactgcatga aacaagcaag gaacccgacg
     6901 tgtccctggg ctccacctgg ctgtccgact ttccccaggc ctgggccgag acaggaggaa
     6961 tgggcctggc cgtgcggcag gcacccctga tcatccctct gaaggccacc tctacacccg
     7021 tgagcatcaa gcagtaccct atgtctcagg aggccagact gggcatcaag cctcacatcc
     7081 agaggctgct ggaccagggc atcctggtgc catgccagag cccctggaac acaccactgc
     7141 tgcccgtgaa gaagccaggc accaatgact atagacccgt gcaggatctg agagaggtga
     7201 acaagagggt ggaggatatc caccccaccg tgcccaaccc ttacaatctg ctgtccggcc
     7261 tgcccccttc tcaccagtgg tatacagtgc tggacctgaa ggatgccttc ttttgtctga
     7321 gactgcaccc taccagccag ccactgttcg cctttgagtg gagggaccct gagatgggca
     7381 tctctggcca gctgacctgg acacgcctgc ctcagggctt caagaatagc ccaacactgt
     7441 ttaacgaggc cctgcaccgc gacctggcag atttccggat ccagcaccca gatctgatcc
     7501 tgctgcagta cgtggacgat ctgctgctgg ccgccaccag cgagctggat tgccagcagg
     7561 gaacacgcgc cctgctgcag accctgggaa acctgggata tagggcatcc gccaagaagg
     7621 cccagatctg tcagaagcag gtgaagtacc tgggctatct gctgaaggag ggccagagat
     7681 ggctgacaga ggccaggaag gagacagtga tgggccagcc aacacccaag accccaagac
     7741 agctgaggga gttcctgggc aaagcaggat tttgcaggct gttcatccca ggattcgcag
     7801 agatggcagc acctctgtac ccactgacca agccgggcac cctgtttaat tggggccctg
     7861 accagcagaa ggcctatcag gagatcaagc aggccctgct gacagcacca gccctgggcc
     7921 tgccagacct gaccaagcct ttcgagctgt ttgtggatga gaagcagggc tacgccaagg
     7981 gcgtgctgac ccagaagctg ggaccatgga gGcggcccgt ggcctatctg tccaagaagc
     8041 tggacccagt ggcagcagga tggccaccat gcctgaggat ggtggcagca atcgccgtgc
     8101 tgacaaagga tgccggcaag ctgaccatgg gacagccact ggtcatcctg gcaccacacg
     8161 cagtggaggc cctggtgaag cagcctccag atcgctggct gtctaacgcc cggatgacac
     8221 actaccaggc cctgctgctg gacaccgatc gcgtgcagtt tggccctgtg gtggccctga
     8281 atccagccac cctgctgcct ctgccagagg agggcctgca gcacaactgt ctggacatcc
     8341 tggcagaggc acacggaaca aggccagacc tgaccgatca gcccctgcct gacgccgatc
     8401 acacatggta taccgatgga agctccctgc tgcaggaggg ccagaggaag gcaggagcag
     8461 cagtgaccac agagacagaa gtgatctggg ccaaggccct gccagcaggc acatccgccc
     8521 agcgggccga gctgatcgcc ctgacccagg ccctgaagat ggccgagggc aagaagctga
     8581 acgtgtacac agactccaga tatgccttcg ccaccgcaca catccacgga gagatctaca
     8641 ggcgccgggg ctggctgacc tctgagggca aggagatcaa gaacaaggat gagatcctgg
     8701 ccctgctgaa ggccctgttt ctgcccaagc ggctgagcat catccactgt cctggacacc
     8761 agaagggaca ctccgccgag gcaaggggca atcggatggc cgaccaggcc gccagaaagg
     8821 ctgctattac tgaaactccc gacacttcca ctctgctgat tgaaaactcc tccccttctg
     8881 gcggctcaaa aagaaccgcc gacggcagcg aattcgagtc tcccaagaag aagaggaaag
     8941 tcggctctgg ccctgccgct aagagagtga agctggacgg ATCCTAACTC GAGCGACGCC
     9001 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG
     9061 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT
     9121 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG
     9181 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATggg ctgaagtgaa
     9241 taaagctcca cacagtcgat gtttctatat gttttaagtc ttaggcatat cggacatcac
     9301 tagtgcatca gcgccctctg ctggttcagt aagaatggtt tccccatata ctgggcaaaa
     9361 ctggattttt gtggtgatga aagggaaaaa aacaaattca gtacaattgg aaagctggtg
     9421 ttgttttaaa actcttgaaa aacactgaat gaaaggaatc acactaaaac tatatgttgc
     9481 aatgttgttg gttaaAACGG ATCTGGCCTC CGCGCCGGGT TTTGGCGCCT CCCGCGGGCG
     9541 CCCCCCTCCT CACGGCGAGC GCTGCCACGT CAGACGAAGG GCGCAGCGAG CGTCCTGATC
     9601 CTTCCGCCCG GACGCTCAGG ACAGCGGCCC GCTGCTCATA AGACTCGGCC TTAGAACCCC
     9661 AGTATCAGCA GAAGGACATT TTAGGACGGG ACTTGGGTGA CTCTAGGGCA CTGGTTTTCT
     9721 TTCCAGAGAG CGGAACAGGC GAGGAAAAGT AGTCCCTTCT CGGCGATTCT GCGGAGGGAT
     9781 CTCCGTGGGG CGGTGAACGC CGATGATTAT ATAAGGACGC GCCGGGTGTG GCACAGCTAG
     9841 TTCCGTCGCA GCCGGGATTT GGGTCGCGGT TCTTGTTTGT GGATCGCTGT GATCGTCACT
     9901 TGGTGAGTAG CGGGCTGCTG GGCTGGCCGG GGCTTTCGTG GCCGCCGGGC CGCTCGGTGG
     9961 GACGGAAGCG TGTGGAGACA CCGCCAAGGG CTGTAGTCTG GGTCCGCGAG CAAGGTTGCC
    10021 CTGAACTGGG GGTTGGGGGG AGCGCAGCAA AATGGCGGCT GTTCCCGAGT CTTGAATGGA
    10081 AGACGCTTGT GAGGCGGGCT GTGAGGTCGT TGAAACAAGG TGGGGGGCAT GGTGGGCGGC
    10141 AAGAACCCAA GGTCTTGAGG CCTTCGCTAA TGCGGGAAAG CTCTTATTCG GGTGAGATGG
    10201 GCTGGGGCAC CATCTGGGGA CCCTGACGTG AAGTTTGTCA CTGACTGGAG AACTCGGTTT
    10261 GTCGTCTGTT GCGGGGGCGG CAGTTATGGC GGTGCCGTTG GGCAGTGCAC CCGTACCTTT
    10321 GGGAGCGCGC GCCCTCGTCG TGTCGTGACG TCACCCGTTC TGTTGGCTTA TAATGCAGGG
    10381 TGGGGCCACC TGCCGGTAGG TGTGCGGTAG GCTTTTCTCC GTCGCAGGAC GCAGGGTTCG
    10441 GGCCTAGGGT AGGCTCTCCT GAATCGACAG GCGCCGGACC TCTGGTGAGG GGAGGGATAA
    10501 GTGAGGCGTC AGTTTCTTTG GTCGGTTTTA TGTACCTATC TTCTTAAGTA GCTGAAGCTC
    10561 CGGTTTTGAA CTATGCGCTC GGGGTTGGCG AGTGTGTTTT GTGAAGTTTT TTAGGCACCT
    10621 TTTGAAATGT AATCATTTGG GTCAATATGT AATTTTCAGT GTTAGACTAG TAAATTGTCC
    10681 GCTAAATTCT GGCCGTTTTT GGCTTTTTTG TTAGACgcca ctatgACCGA GTACAAGCCC
    10741 ACGGTGCGCC TCGCCACCCG CGACGACGTC CCCCGGGCCG TACGCACCCT CGCCGCCGCG
    10801 TTCGCCGACT ACCCCGCCAC GCGCCACACC GTCGACCCGG ACCGCCACAT CGAGCGGGTC
    10861 ACCGAGCTGC AAGAACTCTT CCTCACGCGC GTCGGGCTCG ACATCGGCAA GGTGTGGGTC
    10921 GCGGACGACG GCGCCGCGGT GGCGGTCTGG ACCACGCCGG AGAGCGTCGA AGCGGGGGCG
    10981 GTGTTCGCCG AGATCGGCCC GCGCATGGCC GAGTTGAGCG GTTCCCGGCT GGCCGCGCAG
    11041 CAACAGATGG AAGGCCTCCT GGCGCCGCAC CGGCCCAAGG AGCCCGCGTG GTTCCTGGCC
    11101 ACCGTCGGaG TCTCGCCCGA CCACCAGGGC AAGGGTCTGG GCAGCGCCGT CGTGCTCCCC
    11161 GGAGTGGAGG CGGCCGAGCG CGCCGGGGTG CCCGCCTTCC TGGAaACCTC CGCGCCCCGC
    11221 AACCTCCCCT TCTACGAGCG GCTCGGCTTC ACCGTCACCG CCGACGTCGA GGTGCCCGAA
    11281 GGACCGCGCA CCTGGTGCAT GACCCGCAAG CCCGGTGCAT CCTAACTCGA GATTCACTCC
    11341 TCAGGTGCAG GCTGCCTATC AGAAGGTGGT GGCTGGTGTG GCCAATGCCC TGGCTCACAA
    11401 ATACCACTGA GATCTTTTTC CCTCTGCCAA AAATTATGGG GACATCATGA AGCCCCTTGA
    11461 GCATCTGACT TCTGGCTAAT AAAGGAAATT TATTTTCATT GCAATAGTGT GTTGGAATTT
    11521 TTTGTGTCTC TCACTCGGAA GGACATATGG GAGGGCAAAT CATTTAAAAC ATCAGAATGA
    11581 GTATTTGGTT TAGAGTTTGG CAACATATGc CCATATGCTG GCTGCCATGA ACAAAGGtTG
    11641 GCTATAAAGA GGTCATCAGT ATATGAAACA GCCCCCTGCT GTCCATTCCT TATTCCATAG
    11701 AAAAGCCTTG ACTTGAGGTT AGATTTTTTT TATATTTTGT TTTGTGTTAT TTTTTTCTTT
    11761 AACATCCCTA AAATTTTCCT TACATGTTTT ACTAGCCAGA TTTTTCCTCC TCTCCTGACT
    11821 ACTCCCAGTC ATAGCTGTCC CTCTTCTCTT ATGgAGATCC CTCGACCTGC AGCCCAAGCT
    11881 TGGCGTAATg ctggaaatct gctcgtcagt ggtgctcaca ctgacgaatc atgtacagat
    11941 cataccgatg actgcctggc gactcacaac taagcaagac agccggaacc agcgccggcg
    12001 aacaccactg catatatggc atatcacaac agtccaagca gttacagaga tgttacgaac
    12061 cctgaacagg ttgactatta attgtgtctg cttgatgtgg acaccaggtg gcgctggaca
    12121 tcagatttgg agaggcagtt gtctagggaa ccgggctctg tgccagcgca ggaggcaggc
    12181 tggctctcct attccaggga tgctcatcca ggaaggaaag gttgcatgct ggacacacta
    12241 accttacaGC GGTGGGCTCT ATGGGGTACC cacgcatggt atggatgaac actagttaaa
    12301 agttttgtta ctttatagaa gaaattttga gtttttgttt ttttttaata aataaataaa
    12361 cataaataaa ttgtttgttg aatttattat tagtatgtaa gtgtaaatat aataaaactt
    12421 aatatctatt caaattaata aataaacctc gatatacaga ccgataaaac acatgcgtca
    12481 attttacgca tgattatctt taacgtacgt cacaatatga ttatctttct agggttaatc
    12541 tagtatacgc gtatgcggcc gcttaattaa tccagctttt gttcccttta gtgagggtta
    12601 attCCGAgcg gccgcgtgtt acaaccaatt aaccaattct gattagaaaa actcatcgag
    12661 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
    12721 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
    12781 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
    12841 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
    12901 caaaagctta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
    12961 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgaggcgaaa
    13021 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
    13081 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
    13141 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
    13201 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
    13261 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
    13321 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
    13381 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctggagc aagacgtttc
    13441 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
    13501 tgttcatgat gatatatttt tatcttgtgc aatgtaacat cagagatttt gagacacaac
    13561 gtggctttgt tgaataaatc gaacttttgc tgagttgaag gatcagtcat gaccaaaatc
    13621 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    13681 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
    13741 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
    13801 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
    13861 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
    13921 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
    13981 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
    14041 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
    14101 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
    14161 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
    14221 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
    14281 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
    14341 gcgttatccc ctgattctgt ggataaccgt gcggccgcca attaatacga ctcactatag
    14401 ggcgaattgg ggcgcgccat tctagattaa ccctagaaag atagtctgcg taaaattgac
    14461 gcatgcattc ttgaaatatt gctctctctt tctaaatagc gcgaatccgt cgctgtgcat
    14521 ttaggacatc tcagtcgccg cttggagctc ccgtgaggcg tgcttgtcaa tgcggtaagt
    14581 gtcactgatt ttgaactata acgaccgcgt gagtcaaaat gacgcatgat tatcttttac
    14641 gtgactttta agatttaact catacgataa ttatattgtt atttcatgtt ctacttacgt
    14701 gataacttat tatatatata ttttcttgtt atagatatca actagaatgc tagcatgggc
    14761 ccagatctcc atgggCCCTg aattcgcatc taga  
//
