LOCUS       pCS2-OXER1-mk2          6057 bp ds-DNA     circular     26-AUG-2022
DEFINITION  .
FEATURES             Location/Qualifiers
     source          1..6057
                     /label="source"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    34..52
                     /label="SP6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    35..52
                     /label="Sp6_primer"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer_bind     35..52
                     /label="SP6"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    35..52
                     /label="Sp6"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        35..53
                     /label="SP6 promoter"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    98..1336
                     /label="OXER1"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     misc_feature    1337..1351
                     /label="alox12_C"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1341..1360
                     /label="pr047f mk2 ECOR1-f"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1347..1370
                     /label="mKate2_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1358..1381
                     /label="011_mKate2_F_Sequencing"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    1358..2065
                     /label="mK2"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1402..1421)
                     /label="pr020r mk2-N1 r"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1426..1446
                     /label="013_Casp9_seq_F2"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1680..1698)
                     /label="mK2_NLS_seq_R5"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1705..1723
                     /label="020_MLKL-seqF1"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    complement(1705..1723)
                     /label="020_MLKL-seqF1_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2028..2047
                     /label="pr020f mk2-C1 f"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(2028..2047)
                     /label="pr020f mk2-C1 f_RC"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     polyA_signal    2110..2301
                     /label="SV40 late polyA"
                     /ApEinfo_revcolor="#d59687"
                     /ApEinfo_fwdcolor="#d59687"
     terminator      complement(2116..2235)
                     /label="SV40_PA_terminator"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2130..2149)
                     /label="EBV_rev_primer"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    complement(2130..2151)
                     /label="mK2_F2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2173..2203)
                     /label="mK2_F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2184..2203
                     /label="SV40pA-R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2184..2203)
                     /label="SV40-pA"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    2259..2281
                     /label="pr087r pEGFPC1rev"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    2262..2282
                     /label="065r mTq2 r"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    2274..2290
                     /label="EGFP-C-REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2351..2368)
                     /label="T3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2351..2370)
                     /label="T3"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer_bind     complement(2351..2370)
                     /label="T3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     promoter        complement(2352..2371)
                     /label="T3_promoter"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     promoter        complement(2353..2371)
                     /label="T3 promoter"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    complement(2388..2403)
                     /label="M13R1"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2388..2406)
                     /label="M13_reverse_primer"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     primer_bind     complement(2388..2408)
                     /label="M13-rev"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(2391..2408)
                     /label="M13R2"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2392..2408)
                     /label="M13R"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2405..2427)
                     /label="M13_pUC_rev_primer"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     misc_feature    complement(2405..2427)
                     /label="M13pUC-Rev"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(2406..2425)
                     /label="M13-48REV"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_binding    complement(2414..2436)
                     /label="LacO"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     promoter        complement(2440..2470)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     promoter        complement(2441..2470)
                     /label="lac_promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     rep_origin      complement(2776..3458)
                     /label="ColE1 origin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(2779..3398)
                     /label="pBR322_origin"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(2874..2893)
                     /label="pBR322ori-F"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     gene            complement(3553..4413)
                     /label="Ampicillin gene"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     CDS             complement(3553..4413)
                     /label="Ampicillin"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     CDS             complement(3556..4215)
                     /label="AmpR"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     promoter        complement(4455..4483)
                     /label="AmpR_promoter"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     rep_origin      complement(4676..4982)
                     /label="F1 ori"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     rep_origin      complement(4676..4982)
                     /label="f1_origin"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
     CDS             complement(5001..5069)
                     /label="LacZ alpha"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    5975..6016
                     /label="CMV_fwd_primer"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    6001..6016
                     /label="pr086f CMV promoter f"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atcccatcga tgccaccATG AAGACGATCA TCGCCCTGAG
      121 CTACATCTTC TGCCTGGTAT TCGCCGACTA CAAGGACGAT GATGACGCCA GCATCAGATC
      181 TGAACTCCAC AACCTTTCCA GCCCCTCTCC ATCCCTTTCC AGTTCTGTGC TCCCGCCCAG
      241 TTTTAGTCCA TCTCCGAGTA GCGCCCCATC CGCTTTCACC ACAGTGGGAG GATCTAGCGG
      301 CGGCCCATGC CACCCCACTT CATCAAGCCT CGTGAGCGCC TTTCTTGCCC CTATCCTTGC
      361 CCTGGAGTTC GTACTGGGCC TCGTCGGTAA CTCCCTGGCC CTCTTCATCT TCTGCATCCA
      421 TACGAGACCC TGGACCTCAA ACACAGTCTT CCTGGTTAGC CTGGTGGCAG CTGATTTTCT
      481 GCTCATTTCA AACCTGCCTT TGCGAGTGGA TTACTATCTT TTGCACGAGA CCTGGAGGTT
      541 CGGTGCAGCA GCCTGCAAAG TCAACCTGTT CATGCTGTCC ACCAATCGGA CAGCTAGCGT
      601 GGTCTTTCTG ACCGCAATTG CTTTGAACAG ATATCTTAAG GTCGTACAGC CTCATCACGT
      661 CCTTAGCCGA GCGTCCGTTG GCGCAGCAGC AAGAGTGGCC GGTGGGCTGT GGGTAGGCAT
      721 TCTTCTCCTT AATGGGCACC TCCTTCTTTC TACCTTCAGT GGGCCATCCT GCCTGAGCTA
      781 TCGGGTTGGC ACGAAACCTT CTGCGTCCCT GCGCTGGCAC CAGGCCCTGT ACCTGCTGGA
      841 GTTTTTTCTG CCATTGGCGC TCATTTTGTT CGCCATCGTC TCCATTGGGC TCACAATCCG
      901 CAATCGGGGT CTCGGGGGCC AAGCCGGGCC ACAGCGCGCC ATGAGGGTGC TGGCAATGGT
      961 GGTTGCCGTC TACACTATTT GTTTTCTGCC TAGCATCATT TTTGGAATGG CAAGCATGGT
     1021 GGCATTTTGG CTGTCTGCTT GTAGAAGTCT TGATTTGTGT ACCCAGCTGT TCCATGGTTC
     1081 ATTGGCTTTC ACCTACCTGA ACTCAGTTCT GGATCCCGTT CTGTACTGCT TCTCAAGTCC
     1141 TAACTTCCTT CACCAGAGCC GCGCCCTGCT CGGCCTGACA AGGGGGCGGC AGGGCCCAGT
     1201 GTCCGATGAA TCCTCATACC AGCCCAGCCG GCAGTGGAGG TACAGAGAGG CTAGTCGGAA
     1261 GGCCGAGGCC ATCGGCAAAC TGAAGGTGCA GGGGGAAGTG AGCCTTGAAA AAGAAGGAAG
     1321 TAGCCAAGGC GTCGACggag gaattcgtcc tggatctatg gtgtctaagg gcgaagagct
     1381 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     1441 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     1501 ggccgtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     1561 cggcagcaaa accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     1621 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     1681 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     1741 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggcctccac
     1801 cgagaccctg taccccgctg acggcggcct ggaaggcaga gccgacatgg ccctgaagct
     1861 cgtgggcggg ggccacctga tctgcaactt gaagaccaca tacagatcca agaaacccgc
     1921 taagaacctc aagatgcccg gcgtctacta tgtggacaga agactggaaa gaatcaagga
     1981 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     2041 ccctagcaaa ctggggcaca aactgtaact cgagcctcta gaactatagt gagtcgtatt
     2101 acgtagatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     2161 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     2221 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     2281 gggaggtgtg ggaggttttt taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc
     2341 cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct
     2401 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     2461 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     2521 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     2581 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     2641 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     2701 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     2761 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     2821 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     2881 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     2941 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3001 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     3061 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     3121 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     3181 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
     3241 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     3301 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     3361 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     3421 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     3481 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     3541 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     3601 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     3661 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     3721 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     3781 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     3841 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     3901 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     3961 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4021 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     4081 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     4141 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     4201 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     4261 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     4321 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     4381 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     4441 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     4501 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat
     4561 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc
     4621 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
     4681 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
     4741 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
     4801 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga
     4861 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
     4921 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
     4981 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
     5041 tcggtgcggg cctcttcgct attacgccag tcgaccatag ccaattcaat atggcgtata
     5101 tggactcatg ccaattcaat atggtggatc tggacctgtg ccaattcaat atggcgtata
     5161 tggactcgtg ccaattcaat atggtggatc tggaccccag ccaattcaat atggcggact
     5221 tggcaccatg ccaattcaat atggcggact tggcactgtg ccaactgggg aggggtctac
     5281 ttggcacggt gccaagtttg aggaggggtc ttggccctgt gccaagtccg ccatattgaa
     5341 ttggcatggt gccaataatg gcggccatat tggctatatg ccaggatcaa tatataggca
     5401 atatccaata tggccctatg ccaatatggc tattggccag gttcaatact atgtattggc
     5461 cctatgccat atagtattcc atatatgggt tttcctattg acgtagatag cccctcccaa
     5521 tgggcggtcc catataccat atatggggct tcctaatacc gcccatagcc actcccccat
     5581 tgacgtcaat ggtctctata tatggtcttt cctattgacg tcatatgggc ggtcctattg
     5641 acgtatatgg cgcctccccc attgacgtca attacggtaa atggcccgcc tggctcaatg
     5701 cccattgacg tcaataggac cacccaccat tgacgtcaat gggatggctc attgcccatt
     5761 catatccgtt ctcacgcccc ctattgacgt caatgacggt aaatggccca cttggcagta
     5821 catcaatatc tattaatagt aacttggcaa gtacattact attggaagga cgccagggta
     5881 cattggcagt actcccattg acgtcaatgg cggtaaatgg cccgcgatgg ctgccaagta
     5941 catccccatt gacgtcaatg gggaggggca atgacgcaaa tgggcgttcc attgacgtaa
     6001 atgggcggta ggcgtgccta atgggaggtc tatataagca atgctcgttt agggaac
//