LOCUS       #55.SRE.mCMV.fla        7534 bp DNA     circular SYN 05-FEB-2020
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7534)
  AUTHORS   Ferdinando Pucci
  TITLE     Direct Submission
  JOURNAL   Exported Sep 9, 2024 from SnapGene Viewer 7.2.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7534
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     attenuator      80..285
                     /label=SV40polyA oriR
     CDS             286..446
                     /label=C19
     misc_feature    447..905
                     /label=ageF1 origin
     misc_feature    906..2904
                     /label=C19
     primer_bind     3055..3075
                     /label=p1074 F primer 1 3050-3550
     attenuator      3122..3794
                     /label=V IE-I prom
     primer_bind     3412..3432
                     /label=p1074 F primer 2 3450-3950
     primer_bind     3726..3746
                     /label=p1074 F primer 3 3850-4350
     enhancer        3796..3977
                     /label=5
     attenuator      3978..4128
                     /label=S SL123
     misc_feature    4129..4483
                     /label=4 mgag
     primer_bind     4242..4262
                     /label=p1074 F primer 4 4250-4750
     primer_bind     4646..4666
                     /label=p1074 F primer 5 4250-4750
     primer_bind     4964..4984
                     /label=p1074 F primer 6 5050-5550
     primer_bind     5339..5359
                     /label=p1074 F primer 7 5450-5950
     misc_feature    5502..5503
                     /label=SV40 polyA unidirectional
     enhancer        5509..5671
                     /label=SRE
     promoter        5672..5780
                     /label=mCMV
     regulatory      5787..5795
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5793..5855
                     /codon_start=1
                     /label=SS (Igk)
                     /translation="METDTLLLWVLLLWVPGSTGD"
     CDS             5856..5879
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5895..6335
                     /codon_start=1
                     /label=SrtA
                     /translation="QAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATSEQLNRGVSFA
                     EENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRNVKP
                     TDVGVLDEQKGKDKQLTLITCDDYNEKTGVWETRKIFVATEVK"
     CDS             6393..6539
                     /codon_start=1
                     /label=PDGFR TM
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     primer_bind     6543..6562
                     /label=p1074 F primer 14 8250-8750
     misc_feature    6549..7055
                     /label=WPRE*
                     /note="6bp downstream SacII-site removed by linker insert 
                     (partial homology retained though)"
     primer_bind     6906..6926
                     /label=p1074 F primer 15 8650-9150
     primer_bind     7136..7156
                     /label=p1074 F primer 16 9050-9550
     enhancer        7180..7414
                     /label=3RU5
ORIGIN
        1 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
       61 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc cagttccgcc
      121 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg
      181 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcgtc
      241 gagacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
      301 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      361 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      421 tgcgcagcct gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg
      481 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
      541 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
      601 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
      661 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
      721 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
      781 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
      841 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa
      901 tttcccaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
      961 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     1021 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     1081 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     1141 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     1201 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     1261 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     1321 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     1381 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     1441 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     1501 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     1561 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     1621 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     1681 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     1741 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     1801 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     1861 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     1921 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     1981 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     2041 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     2101 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     2161 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
     2221 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     2281 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     2341 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     2401 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     2461 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     2521 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     2581 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     2641 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     2701 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     2761 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     2821 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     2881 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     2941 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     3001 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     3061 tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg agctgcaagc
     3121 ttggccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     3181 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     3241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     3301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     3361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     3421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     3481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     3541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     3601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     3661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     3721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     3781 tcgtttagtg aaccggggtc tctctggtta gaccagatct gagcctggga gctctctggc
     3841 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     3901 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     3961 tggaaaatct ctagcagtgg cgcccgaaca gggacctgaa agcgaaaggg aaaccagagc
     4021 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     4081 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     4141 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     4201 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     4261 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     4321 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     4381 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     4441 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     4501 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     4561 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     4621 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     4681 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     4741 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     4801 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     4861 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     4921 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     4981 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     5041 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     5101 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     5161 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     5221 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     5281 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     5341 aacggatctc gacggtatcg gttaactttt aaaagaaaag gggggattgg ggggtacagt
     5401 gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa
     5461 caaattacaa aaattcaaaa ttttatcgat cacgagacta gcctcgagag ccagacaggg
     5521 taagccagac atcagcagcc agacaatcag agccagacac attcagccag acataacgag
     5581 ccagacagtc caagccagac aacgttagcc agacatttgt agccagacat tgaaagccag
     5641 acagaaggag ccagacatac tgagccagac agtacggtgg gaggcctata taagcagagc
     5701 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     5761 aagacaccgg gaccgatcca ccggtcgcca ccatggagac tgacacattg cttctgtggg
     5821 tcctccttct ttgggttcca ggaagcactg gcgacgacta caaggacgac gatgataaag
     5881 ggtccggcct ggaacaagct aaaccccaaa ttcccaagga taaaagcaag gtagctggat
     5941 acatagaaat acctgatgcc gatattaagg aacccgttta ccccggtcca gctacttctg
     6001 aacaactgaa tcgcggagtc tctttcgcag aagagaacga gtccttggat gaccaaaaca
     6061 tttcaatcgc aggtcacact tttatagata ggccaaacta ccaatttact aatctgaaag
     6121 ctgctaagaa gggttctatg gtctacttta aggtagggaa cgagacccgg aagtacaaaa
     6181 tgaccagtat tcgcaatgtg aagcccacag atgtgggtgt acttgatgaa caaaagggaa
     6241 aagacaaaca gttgacactg ataacttgcg acgattacaa cgagaagact ggagtttggg
     6301 agacacgaaa gatctttgta gcaacagagg tcaaaggtag taccagcgga tctggcaagc
     6361 ccggatccgg cgagggcagt acaaaaggta atgccgtggg tcaagacaca caagaggtta
     6421 ttgtcgtgcc ccacagtctt ccattcaagg ttgtcgtcat tagtgccatc ttggcccttg
     6481 tcgtcctcac tattatctcc ctgataatat tgataatgct ctggcagaag aaacctcgct
     6541 aggtcgacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     6601 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     6661 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     6721 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     6781 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     6841 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     6901 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     6961 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     7021 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctcta gagcctcttc
     7081 cgcgtcttcg ccttcccggg tcgagctcgg tacctttaag accaatgact tacaaggcag
     7141 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7201 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     7261 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     7321 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     7381 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
     7441 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     7501 cttataatgg ttacaaataa agcaatagca tcac
//
