LOCUS       pcmk83-gidb-m-ss        4684 bp DNA     circular SYN 11-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 11, 2024 from SnapGene Viewer 7.0.2
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4684
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      98..1010
                     /label=P15A ori
     misc_feature    1130..1656
                     /label=homology arm
     misc_feature    1130..1491
                     /label=gidb
     misc_feature    complement(1668..2942)
                     /label=slr0214 M.Ssp6803I
     CDS             complement(1668..2942)
                     /codon_start=1
                     /label=Translation 2942-1668
                     /translation="MARPIAIDLFAGCGGMSLGLEAAGFDIAAAVEFDAVHCLVHHHNF
                     PYGVTICRDIALVSAGEILRKLNNKGYSSDIDLIAGGPPCQGFSLMGKRQLDDPRNSLV
                     FEYVRMIRDLKPKYFLFENVPGMRSGQHKKFLEELVREFETIGYHIEKPILVLDASLYG
                     APQKRKRLIILGSRKDVKPVTYPLPLYFDVAELKQSNCSEKSVSNIAYKPPFTCGEAID
                     DLVDHTPYAVGEDQGIPSELLDYSALRKSYAIHANGAFSLCHKRQVNNLVYGHIGSQHT
                     EKSMKRFADTVPGTVERTSRFLRLSKTGLCNTLRAGTASDKGAYTAPRPIHYALPRCIT
                     VREAARLHTYPDWFQFHNTIWHGFREIGNSVVPLLAKVLGEQVMAALNCSVEDLPVYEL
                     EPQDRTLLHYNLPQASRYLCAKSHS"
     CDS             2457..2468
                     /label=Factor Xa site
     promoter        complement(2956..3005)
                     /label=tet resistance promoter-constitutive
                     /label=tet resistance promoter
     misc_feature    complement(2958..2965)
                     /label=crp binding site
     misc_feature    complement(2973..2981)
                     /label=-10
     misc_feature    complement(2998..3003)
                     /label=-35
     misc_feature    3049..3558
                     /label=homology arm
     misc_feature    3058..3063
                     /label=-35
     misc_feature    3079..3086
                     /label=rpod16
     misc_feature    3105..3110
                     /label=-35
     misc_feature    3125..3133
                     /label=-10
     misc_feature    3178..3558
                     /label=atpI
     promoter        3646..3750
                     /label=AmpR promoter
     CDS             3751..4611
                     /label=AmpR
ORIGIN
        1 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
       61 gagcgtcaga ccccgtagaa aagatcaaag gatcttcgcg ctagcggagt gtatactggc
      121 ttactatgtt ggcactgatg agggtgtcag tgaagtgctt catgtggcag gagaaaaaag
      181 gctgcaccgg tgcgtcagca gaatatgtga tacaggatat attccgcttc ctcgctcact
      241 gactcgctac gctcggtcgt tcgactgcgg cgagcggaaa tggcttacga acggggcgga
      301 gatttcctgg aagatgccag gaagatactt aacagggaag tgagagggcc gcggcaaagc
      361 cgtttttcca taggctccgc ccccctgaca agcatcacga aatctgacgc tcaaatcagt
      421 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tcccctggcg gctccctcgt
      481 gcgctctcct gttcctgcct ttcggtttac cggtgtcatt ccgctgttat ggccgcgttt
      541 gtctcattcc acgcctgaca ctcagttccg ggtaggcagt tcgctccaag ctggactgta
      601 tgcacgaacc ccccgttcag tccgaccgct gcgccttatc cggtaactat cgtcttgagt
      661 ccaacccgga aagacatgca aaagcaccac tggcagcagc cactggtaat tgatttagag
      721 gagttagtct tgaagtcatg cgccggttaa ggctaaactg aaaggacaag ttttggtgac
      781 tgcgctcctc caagccagtt acctcggttc aaagagttgg tagctcagag aaccttcgaa
      841 aaaccgccct gcaaggcggt tttttcgttt tcagagcaag agattacgcg cagaccaaaa
      901 cgatctcaag aagatcatct tattaatcag ataaaatatt tctagatttc agtgcaattt
      961 atctcttcaa atgtagcacc tgaagtcagc cccatacgat ataagttgta aacgccagca
     1021 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1081 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagcc tgaagcccat
     1141 ttcactctgt tggatagcct tggtaaacgc gtgcgtttcc ttcgtcaggt gcaacatgag
     1201 cttaaactgg agaatattga accagtacag agcagggtag aagagtttcc ttcagagccg
     1261 ccatttgatg gcgtaattag ccgcgctttt gcctctctga acgatatggt gagctggtgc
     1321 caccatcttc ctggtgagca aggccgtttc tacgcgctga aagggcaaat gccggaagat
     1381 gaaatcgctt tgttgcccga agaatatcag gtcgaatcag tggttaaact tcaggttcca
     1441 gccctggatg gcgaacgtca tctggtggtg attaaagcaa ataaaattta atttttatca
     1501 aaaaaatcat aaaaaattga ccggttagac tgttaacaac aaccaggttt tctactgata
     1561 taactggtta catttaacgc cacgttcact cttttgcatc aacaagataa cgtggctttt
     1621 tttggtaagc agaaaataag tcattagtga aaatatggct aactgcttta ggaatgggat
     1681 ttggcacata aatatcgact agcctggggc aaattgtaat gtagtaatgt tcggtcttgg
     1741 ggctccagtt cataaaccgg caaatcctca acagaacaat tcaatgcggc cataacctgt
     1801 tctcccaaaa ccttagccag caaaggtacg acggagttac caatttcccg aaagccgtgc
     1861 cagatggtgt tgtggaactg aaaccaatcc ggataggtat ggagtctggc cgcttctctc
     1921 acagttatac atctaggtaa ggcgtagtgt attggcctag gggcggtata agcaccctta
     1981 tcactagcag tgccggccct cagggtatta cataaaccag tttttgatag ccgcagaaag
     2041 cgactggttc tttccaccgt tccagggaca gtatccgcaa atcttttcat tgatttttca
     2101 gtatgttgag aaccaatatg gccataaact aaattattaa cttggcgctt atggcagagg
     2161 gaaaatgctc catttgcatg gatggcataa ctttttctca gggctgaata atccaataat
     2221 tctgatggaa taccttggtc ttctcctact gcatagggtg tatggtcaac tagatcatca
     2281 atggcttctc cacaggtaaa tggcggttta taagctatgt tagaaactga tttttcagaa
     2341 caattacttt gttttaattc agcaacgtca aaatagagtg gaaggggata ggtgacaggc
     2401 ttgacatctt ttctggagcc gagaataatc aacctttttc gtttttgggg agcaccatag
     2461 agggacgcat ctagcactaa aattggtttt tcgatgtggt aaccaatggt ttcaaattcc
     2521 ctaactaatt cctctaaaaa ttttttatgt tgccccgatc gcataccggg aacattttca
     2581 aataaaaaat atttgggttt gagatcccga atcattcgca catattcaaa caccaaggag
     2641 ttacgaggat catccaactg ccttttaccc atgagggaaa agccttgaca gggagggcct
     2701 ccagcaatca ggtctatatc gcttgaataa cctttattat ttaactttct tagaatttcg
     2761 cccgcactta ccagagcaat atcccgacag atagtaacac catagggaaa attatgatga
     2821 tggaccaaac aatgcactgc atcaaattcc accgcggcgg caatatcaaa tccagcggct
     2881 tctaaaccca aggacatgcc accacagccc gcaaacaagt cgatggcaat gggtctggcc
     2941 atggtttttc ctcctagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga
     3001 taagctgtca aacatgagaa ttacaactta tatcgtatgg ggctgacata cttattgttt
     3061 gaaatcacgg gggcgcaccg tataatttga ccgctttttg atgcttgact ctaagcctta
     3121 aagaaagttt tatacgacac gcggcatacc tcgaagggag caggagtgaa aaacgtgatg
     3181 tctgtgtcgc tcgtgagtcg aaacgttgct cggaagcttc tgctcgttca gttactggtg
     3241 gtgatagcaa gtggattgct gttcagcctc aaagacccct tctggggcgt ctctgcaata
     3301 agcgggggcc tggcagtctt tctgcctaac gttttgttta tgatatttgc ctggcgtcac
     3361 caggcgcata caccagcgaa aggccgggtg gcctggacat tcgcatttgg cgaagctttc
     3421 aaagttctgg cgatgttggt gttactggtg gtggcgttgg cggttttaaa ggcggtattc
     3481 ttgccgctga tcgttacgtg ggttttggtg ctggtggttc agatactggc accggctgta
     3541 attaacaaca aagggtaact cgtttcgata cgcctatttt tataggttaa tgtcatgata
     3601 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     3661 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
     3721 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
     3781 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
     3841 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
     3901 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
     3961 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
     4021 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
     4081 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
     4141 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
     4201 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
     4261 ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa
     4321 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
     4381 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
     4441 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
     4501 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
     4561 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
     4621 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
     4681 tagg
//
