LOCUS       24AAW5NC_NW03_N-term-His_pcDNA3.4-TOPO        7576 bp  DNA     circular  31-JUL-2014
FEATURES             Location/Qualifiers
     promoter        47..726
                     /label="CMV\promoter"
                     /vntifkey="29"
     primer_bind     584..604
                     /label="CMV\for\primer\binding\site"
                     /vntifkey="28"
     CDS             2348..2945
                     /label="WPRE"
                     /vntifkey="21"
     primer_bind     complement(2388..2410)
                     /label="pcDNA3.4\rev\primer\binding\site"
                     /vntifkey="28"
     CDS             2949..3220
                     /label="TK\pA\signal"
                     /vntifkey="25"
     promoter        3689..4058
                     /label="SV40\early\promoter"
                     /vntifkey="29"
     CDS             4094..4888
                     /label="Neo-R"
                     /vntifkey="22"
     CDS             5064..5194
                     /label="SV40\pA\signal"
                     /vntifkey="25"
     rep_origin      complement(5577..6250)
                     /label="pUC\ori"
                     /vntifkey="33"
     CDS             complement(6395..7255)
                     /label="Amp-R"
                     /vntifkey="22"
     promoter        complement(7256..7354)
                     /label="bla\promoter"
                     /vntifkey="30"
     gene            742..2307
                     /label="NW03_N-term-His"
ORIGIN
        1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA
       61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG
      121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC
      181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA
      241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT
      301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC
      361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT
      421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA
      481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT
      541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG
      601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA GATCGCCTGG
      661 AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC CAGCCTCCGG
      721 ACTCTAGAGG ATCGAACCCT TGGATCCGCC ACCATGTACA GAATGCAGCT GCTGAGCTGT
      781 ATCGCCCTGT CTCTGGCCCT GGTCACCAAT TCTCACCACC ACCATCACCA CCTGATTCCT
      841 CTGAAGCCCC TGACCGAGAC AGAGCTGCGG ATCAAAGAGA TCATCGAGAA GCTGGACCAG
      901 CAGATCCCTC CTCGGCCTTT CACACACGTG AACACCACAA CAAGCGCCAC ACACAGCACC
      961 GCCACAATCC TGAATCCTCG GGATACCTAC TGCAGAGGCG ACCAGCTGGA CATTCTGCTG
     1021 GAAGTGCGGG ATCACCTGGG ACAGAGAAAG CAGTACGGCG GCGATTTCCT GAGAGCCAGA
     1081 ATGAGCAGTC CTGCTCTGAC AGCCGGCGCT AGCGGCAAAG TGATGGACTT CAACAACGGC
     1141 ACCTACCTGG TGTCTTTCAC CCTGTTCTGG GAAGGCCAGG TTTCCCTGAG CCTGCTGCTG
     1201 ATTCATCCTT CTGAAGGCGC CAGCGCTCTG TGGCGAGCCA GAAATCAGGG CTACGACAAG
     1261 ATCATCTTCA AGGGCAAGTT CGTGAACGGC ACCAGCCACG TGTTCACCGA GTGTGGCCTG
     1321 ACACTGAACA GCAATGCCGA GCTGTGCGAG TACCTGGACG ACAGAGATCA AGAGGCCTTC
     1381 TACTGCATGA AGCCCCAGCA CATGCCTTGC GAGGCCCTGA CCTACATGAC CACCAGAAAC
     1441 AGAGAGGTGT CCTACCTGAC CGACAAAGAG AACAGCCTGT TCCACCGGTC CAAAGTGGGC
     1501 GTCGAGATGA TGAAGGACCG GAAGCACATC GACGTGACCA ACTGCAACAA GCGGGAAAAG
     1561 ATCGAAGAGA CATGCCAAGT GGGAATGAAG CCTCCTGTGC CTGGCGGCTA TACACTGCAA
     1621 GGCAAGTGGA TCACCACGTT CTGCAATCAA GTGCAGCTCG ACACCATCAA GATCAACGGC
     1681 TGCCTGAAGG GCAAGCTGAT CTACCTGCTG GGCGATAGCA CCCTGCGGCA GTGGATCTAC
     1741 TACTTCCCCA AGGTGGTCAA GACCCTGAAG TTCTTCGACC TGCACGAGAC AGGGATCTTC
     1801 AAGAAGCACC TCCTGCTGGA CGCCGAGAGA CACACACAGA TCCAGTGGAA GAAGCACAGC
     1861 TACCCCTTCG TGACCTTCCA GCTGTACTCC CTGATCGACC ACGACTACAT CCCCAGAGAG
     1921 ATCGACAGAC TGAGCGGCGA CAAGAACACC GCCATCGTGA TCACCTTCGG CCAGCACTTC
     1981 AGACCCTTTC CAATCGACAT CTTCATTCGG AGAGCCATCG GCGTGCAGAA GGCCATCGAG
     2041 AGACTGTTTC TGAGAAGCCC CGCCACCAAA GTGATCATCA AGACCGAGAA CATCCGCGAG
     2101 ATGCACATCG AAACCGAGAG ATTCGGCGAC TTTCACGGCT ACATCCACTA CCTGATCATG
     2161 AAGGACATCT TTAAGGACCT GAACGTGGGC ATCATCGACG CCTGGGACAT GACAATCGCC
     2221 TACGGCACCG ATACCATCCA TCCTCCAGAC CACGTGATCG GCAACCAGAT CAACATGTTC
     2281 CTGAACTACA TCTGCTGATG ACTCGAGAAG GGTTCGATCC CTACCGGTTA GTAATGAGTT
     2341 TGATATCTCG ACAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT
     2401 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT
     2461 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT
     2521 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC
     2581 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT
     2641 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA
     2701 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAGCT GACGTCCTTT
     2761 CCATGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC
     2821 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT
     2881 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG
     2941 CCTGGAACGG GGGAGGCTAA CTGAAACACG GAAGGAGACA ATACCGGAAG GAACCCGCGC
     3001 TATGACGGCA ATAAAAAGAC AGAATAAAAC GCACGGGTGT TGGGTCGTTT GTTCATAAAC
     3061 GCGGGGTTCG GTCCCAGGGC TGGCACTCTG TCGATACCCC ACCGAGACCC CATTGGGGCC
     3121 AATACGCCCG CGTTTCTTCC TTTTCCCCAC CCCACCCCCC AAGTTCGGGT GAAGGCCCAG
     3181 GGCTCGCAGC CAACGTCGGG GCGGCAGGCC CTGCCATAGC AGATCTGCGC AGCTGGGGCT
     3241 CTAGGGGGTA TCCCCACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA
     3301 CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC
     3361 CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT
     3421 TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG
     3481 GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA
     3541 CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT
     3601 ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA
     3661 TTTAACAAAA ATTTAACGCG AATTAATTCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA
     3721 GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC
     3781 CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA
     3841 TTAGTCAGCA ACCATAGTCC CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG
     3901 TTCCGCCCAT TCTCCGCCCC ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC
     3961 CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT
     4021 TTGCAAAAAG CTCCCGGGAG CTTGTATATC CATTTTCGGA TCTGATCAAG AGACAGGATG
     4081 AGGATCGTTT CGCATGATTG AACAAGATGG ATTGCACGCA GGTTCTCCGG CCGCTTGGGT
     4141 GGAGAGGCTA TTCGGCTATG ACTGGGCACA ACAGACAATC GGCTGCTCTG ATGCCGCCGT
     4201 GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT TCTTTTTGTC AAGACCGACC TGTCCGGTGC
     4261 CCTGAATGAA CTGCAGGACG AGGCAGCGCG GCTATCGTGG CTGGCCACGA CGGGCGTTCC
     4321 TTGCGCAGCT GTGCTCGACG TTGTCACTGA AGCGGGAAGG GACTGGCTGC TATTGGGCGA
     4381 AGTGCCGGGG CAGGATCTCC TGTCATCTCA CCTTGCTCCT GCCGAGAAAG TATCCATCAT
     4441 GGCTGATGCA ATGCGGCGGC TGCATACGCT TGATCCGGCT ACCTGCCCAT TCGACCACCA
     4501 AGCGAAACAT CGCATCGAGC GAGCACGTAC TCGGATGGAA GCCGGTCTTG TCGATCAGGA
     4561 TGATCTGGAC GAAGAGCATC AGGGGCTCGC GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC
     4621 GCGCATGCCC GACGGCGAGG ATCTCGTCGT GACCCATGGC GATGCCTGCT TGCCGAATAT
     4681 CATGGTGGAA AATGGCCGCT TTTCTGGATT CATCGACTGT GGCCGGCTGG GTGTGGCGGA
     4741 CCGCTATCAG GACATAGCGT TGGCTACCCG TGATATTGCT GAAGAGCTTG GCGGCGAATG
     4801 GGCTGACCGC TTCCTCGTGC TTTACGGTAT CGCCGCTCCC GATTCGCAGC GCATCGCCTT
     4861 CTATCGCCTT CTTGACGAGT TCTTCTGAGC GGGACTCTGG GGTTCGCGAA ATGACCGACC
     4921 AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT
     4981 TGGGCTTCGG AATCGTTTTC CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA
     5041 TGCTGGAGTT CTTCGCCCAC CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA
     5101 GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT
     5161 TGTCCAAACT CATCAATGTA TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT
     5221 TGGCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC
     5281 ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC
     5341 TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC
     5401 TGCATTAATG AATCGGCCAA CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG
     5461 CTTCCTCGCT CACTGACTCG CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC
     5521 ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT
     5581 GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC
     5641 ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA
     5701 ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC
     5761 CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG
     5821 CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC
     5881 TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC
     5941 GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA
     6001 GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT
     6061 ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG
     6121 GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT
     6181 TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT
     6241 TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA
     6301 GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA
     6361 TCTAAAGTAT ATATGAGTAA ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC
     6421 CTATCTCAGC GATCTGTCTA TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA
     6481 TAACTACGAT ACGGGAGGGC TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC
     6541 CACGCTCACC GGCTCCAGAT TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA
     6601 GAAGTGGTCC TGCAACTTTA TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA
     6661 GAGTAAGTAG TTCGCCAGTT AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG
     6721 TGGTGTCACG CTCGTCGTTT GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC
     6781 GAGTTACATG ATCCCCCATG TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG
     6841 TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT
     6901 CTCTTACTGT CATGCCATCC GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT
     6961 CATTCTGAGA ATAGTGTATG CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA
     7021 ATACCGCGCC ACATAGCAGA ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC
     7081 GAAAACTCTC AAGGATCTTA CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC
     7141 CCAACTGATC TTCAGCATCT TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA
     7201 GGCAAAATGC CGCAAAAAAG GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT
     7261 TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT
     7321 TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC
     7381 CACCTGACGT CGACGGATCG GGAGATCTCC CGATCCCCTA TGGTGCACTC TCAGTACAAT
     7441 CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC
     7501 TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT
     7561 GAAGAATCTG CTTAGG
//
