LOCUS       Exported                7930 bp ds-DNA     circular SYN 12-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...m-i76xy3z
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Bacterial expression vector with T7lac promoter, adds N-terminal His
            tag, thrombin cleavage site, internal T7 epitope tag, N-terminal His
            tag
             kanamycin resistance
             restriction enzyme        cloning. Cut with NdeI
FEATURES             Location/Qualifiers
     source          1..7930
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    148..830
                     /standard_name="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    208..827
                     /standard_name="pBR322_origin"
                     /label=pBR322_origin
     misc_feature    complement(2242..3333)
                     /standard_name="lacI"
                     /label=lacI
     misc_feature    3710..3726
                     /standard_name="T7 promoter"
                     /label=T7 promoter
     misc_feature    3729..3756
                     /standard_name="lacO"
                     /label=lacO
     misc_feature    3729..3753
                     /standard_name="lac operator"
                     /label=lac operator
     CDS             3798..6494
                     /codon_start=1
                     /standard_name="Translation 3798-6494"
                     /label=Translation 3798-6494
                     /translation="MGSSHHHHHHSSGLVPRGSHMEESLVLEMLQTGKTATWPAKNQLQ
                     ESGEVVDKEVKGTPSTDRTRRMKERFMNAKCKMDMEAPIAYTKAWREHEGKPLYVRRGL
                     AYKYMLEHLTPAIREDELITMSKTRYDRGATQVPQFATDFMISFLTQAEDQKEEAKLYS
                     VEGKDEAHTVEEEGWTKVGQLFSIREEEVKPMLEVLEYWKTRCVENVSDDWMKTSFPYY
                     QDYVNAKKVGLFPGSGLHAGCDGRWIPAYDVALGGLNRVIEECREKIEKTVVTTKEVAD
                     KVFFWQGCIYACEGAIAWAHNYAVEARRLAETAVEPRKTELLEMAERLDRVPAEAPRNF
                     MEAVQALWTTQILVISDSLALGVSPGRWGKFLEPYYEKDLAEGRITKGQALEVMELLRI
                     KFSTEEYITPSLWAAMASSNSFMNLAVGGLDPKTGKCTDNEIEDLILEAGINMPTPQPT
                     LSILLSDKTTDHLAIKAAECTKAGNGYPAWFNYDMMVQHNLAMYRDEGITLEDARNCAL
                     SGCVENGLAGTGHPIAHPAFYNEGKTIELACNEGVDPRTGIKVMDGIVPIKTYEDLWDN
                     FIKIREHFMHVYMQYWNEVVACQRDIHPKIMGSVLMHDCIESGRPVDNLGCRYNGSVTL
                     LDSGTVNVVNGLAAIKKLVFEDHKYTWDEFKEAMDNNFGFVLGAEKGNFSMLNQEIDPE
                     KHMKYAHIHRDVLNAPKFGNDDDFVDDIFVDLWHDYDRVTASETTYNGYRWITAALSIS
                     AHGPHGRVTGATPDGRLSGVTLCDGILSASPGTDVNGPIALIRSGVKLDPTEFASVQLN
                     MKFHPTAIRGDEGSRNFVDFIHSYFQMGGYHVQFNIVDSKMLRDAQDHPQNYRDLMVRV
                     AGFSAYWNELGKPIQDEVIARTEYDAL"
     misc_feature    3810..3827
                     /standard_name="6xHis Tag"
                     /label=6xHis Tag
     CDS             3858..6494
                     /codon_start=1
                     /standard_name="Translation 3858-6494"
                     /label=Translation 3858-6494
                     /translation="MEESLVLEMLQTGKTATWPAKNQLQESGEVVDKEVKGTPSTDRTR
                     RMKERFMNAKCKMDMEAPIAYTKAWREHEGKPLYVRRGLAYKYMLEHLTPAIREDELIT
                     MSKTRYDRGATQVPQFATDFMISFLTQAEDQKEEAKLYSVEGKDEAHTVEEEGWTKVGQ
                     LFSIREEEVKPMLEVLEYWKTRCVENVSDDWMKTSFPYYQDYVNAKKVGLFPGSGLHAG
                     CDGRWIPAYDVALGGLNRVIEECREKIEKTVVTTKEVADKVFFWQGCIYACEGAIAWAH
                     NYAVEARRLAETAVEPRKTELLEMAERLDRVPAEAPRNFMEAVQALWTTQILVISDSLA
                     LGVSPGRWGKFLEPYYEKDLAEGRITKGQALEVMELLRIKFSTEEYITPSLWAAMASSN
                     SFMNLAVGGLDPKTGKCTDNEIEDLILEAGINMPTPQPTLSILLSDKTTDHLAIKAAEC
                     TKAGNGYPAWFNYDMMVQHNLAMYRDEGITLEDARNCALSGCVENGLAGTGHPIAHPAF
                     YNEGKTIELACNEGVDPRTGIKVMDGIVPIKTYEDLWDNFIKIREHFMHVYMQYWNEVV
                     ACQRDIHPKIMGSVLMHDCIESGRPVDNLGCRYNGSVTLLDSGTVNVVNGLAAIKKLVF
                     EDHKYTWDEFKEAMDNNFGFVLGAEKGNFSMLNQEIDPEKHMKYAHIHRDVLNAPKFGN
                     DDDFVDDIFVDLWHDYDRVTASETTYNGYRWITAALSISAHGPHGRVTGATPDGRLSGV
                     TLCDGILSASPGTDVNGPIALIRSGVKLDPTEFASVQLNMKFHPTAIRGDEGSRNFVDF
                     IHSYFQMGGYHVQFNIVDSKMLRDAQDHPQNYRDLMVRVAGFSAYWNELGKPIQDEVIA
                     RTEYDAL"
     misc_feature    3858..6494
                     /standard_name="O.uli IAD"
                     /label=O.uli IAD
     misc_feature    6501..6518
                     /standard_name="6xHis Tag"
                     /label=6xHis Tag
     misc_feature    6585..6631
                     /standard_name="T7 terminator"
                     /label=T7 terminator
     misc_feature    complement(join(7217..7930,1..102))
                     /standard_name="KanR"
                     /label=KanR
ORIGIN
        1 cccgacatta tcgcgagccc atttataccc atataaatca gcatccatgt tggaatttaa
       61 tcgcggccta gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
      121 gtttatgtaa gcagacagtt ttattgttca tgaccaaaat cccttaacgt gagttttcgt
      181 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
      241 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
      301 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
      361 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
      421 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
      481 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
      541 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
      601 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
      661 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
      721 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
      781 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
      841 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      901 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
      961 agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta
     1021 cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga
     1081 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     1141 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     1201 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     1261 tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca
     1321 cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc
     1381 tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc
     1441 tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg
     1501 ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa
     1561 caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc
     1621 ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc
     1681 cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa
     1741 ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac
     1801 gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc
     1861 cgggtcctca acgacaggag cacgatcatg cgcacccgtg gggccgccat gccggcgata
     1921 atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg
     1981 gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag
     2041 cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata
     2101 aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg
     2161 actgggttga aggctctcaa gggcatcggt cgagatcccg gtgcctaatg agtgagctaa
     2221 cttacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     2281 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgccagggt
     2341 ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg cctggccctg
     2401 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
     2461 ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc ccactaccga
     2521 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
     2581 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
     2641 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
     2701 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
     2761 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
     2821 tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
     2881 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
     2941 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
     3001 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
     3061 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
     3121 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
     3181 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
     3241 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
     3301 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     3361 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     3421 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     3481 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     3541 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     3601 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     3661 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
     3721 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
     3781 taagaaggag atataccatg ggcagcagcc atcatcatca tcatcacagc agcggcctgg
     3841 tgccgcgcgg cagccatatg gaggagtctc tggttctcga gatgctgcag accggtaaga
     3901 cggccacgtg gccggcaaag aatcagctgc aggaaagcgg cgaggtcgtc gacaaggagg
     3961 tcaagggcac tccttcgacc gaccgcacca ggcgcatgaa ggaacgcttc atgaatgcca
     4021 agtgcaagat ggacatggag gcgcccatcg catataccaa ggcatggcgc gagcatgaag
     4081 gcaagccgct ttatgtccgc agggggcttg cctataagta tatgctcgag cacctcacgc
     4141 cggctatccg tgaggacgag ctcatcacca tgtccaagac gcgctatgac cgtggtgcca
     4201 cccaggtccc ccaattcgcc actgacttca tgatctcctt cctcacccag gccgaagacc
     4261 agaaggaaga ggcaaagctg tattccgtcg agggcaagga tgaggcccat acggtcgaag
     4321 aggaagggtg gaccaaggtc ggccagctgt tctccatccg cgaggaggag gtcaagccga
     4381 tgcttgaggt cctcgagtat tggaagacgc gctgcgttga gaacgtgtcc gacgattgga
     4441 tgaagacctc cttcccctac taccaggact acgtcaacgc aaagaaggtc ggcctcttcc
     4501 cggggtcggg cctgcatgcc ggctgtgacg gccgctggat tcccgcctat gacgttgccc
     4561 tcgggggcct caacagggta atcgaggagt gccgcgagaa gatcgagaag accgtggtga
     4621 ccaccaagga ggtcgccgac aaggtcttct tctggcaagg ctgcatctat gcctgcgagg
     4681 gcgcgatcgc ctgggcccac aactatgcgg tcgaggcccg gcgccttgcc gagactgcgg
     4741 tcgagccacg caagaccgag cttctcgaga tggcagaacg cctcgatcgc gtccctgccg
     4801 aagcaccccg caacttcatg gaagccgtcc aggccctttg gaccacccag atcctcgtca
     4861 tctccgactc ccttgccctc ggcgtctccc cgggccgttg gggcaagttc cttgagcctt
     4921 attacgagaa ggatcttgcc gaggggcgca tcaccaaggg acaggccctt gaggtcatgg
     4981 agctgctccg tatcaaattc tcgactgagg agtacatcac cccgtccctc tgggcggcca
     5041 tggcctcctc gaactcgttc atgaaccttg ccgttggcgg cctcgatccc aagacgggca
     5101 agtgcaccga caacgagatc gaggacctca tcctcgaggc cggcatcaac atgcccaccc
     5161 cgcaaccgac cctctcgatc cttctctccg acaagacgac agaccatctc gccatcaagg
     5221 ccgccgagtg caccaaggcc ggcaacgggt atcccgcgtg gtttaactat gacatgatgg
     5281 tccaacacaa cctcgctatg tatcgcgacg agggcatcac ccttgaggac gcccgcaact
     5341 gtgcgctctc cggctgcgtc gagaacggcc tcgccggtac cggtcacccc atcgcccacc
     5401 cggccttcta caacgagggc aagaccatcg agcttgcctg caacgaaggc gtcgaccccc
     5461 gcacgggcat caaggtcatg gatggcatcg tccccatcaa gacgtacgag gacctttggg
     5521 acaacttcat caagatccgc gagcacttca tgcatgtcta catgcagtac tggaatgagg
     5581 tcgtcgcctg ccagcgcgac atccacccca agatcatggg ctccgtcctc atgcatgact
     5641 gcatcgagtc cggccgcccg gttgacaatc tcggctgccg ctacaacggt tccgtcacgc
     5701 tgctcgactc cggtacagtt aacgtggtca acggtctcgc ggccatcaag aagctcgtct
     5761 tcgaggacca caagtacact tgggatgagt tcaaggaggc catggacaat aacttcggtt
     5821 tcgtgcttgg tgccgagaag ggcaacttct ccatgctcaa ccaggagatc gaccctgaaa
     5881 agcacatgaa gtacgcccat atccaccgag atgtcctcaa cgcacccaag tttggcaacg
     5941 acgatgactt cgttgacgac atctttgtcg acctctggca tgactacgac cgcgttaccg
     6001 cgtccgaaac cacctacaac ggctaccgct ggatcaccgc cgcgctttcc atatccgccc
     6061 atggcccgca tggtcgtgtg accggcgcta cccctgacgg tcgtctctcc ggagtgacgc
     6121 tgtgcgacgg catcctgtcc gcctcgcctg gcaccgacgt gaacggtccg atcgccctca
     6181 tccgctccgg cgtgaagctg gatccgaccg agttcgcctc ggtgcagctc aatatgaagt
     6241 tccatccgac tgccatccgc ggtgacgagg gctcgaggaa cttcgtggac ttcatccaca
     6301 gctacttcca gatgggcggc tatcacgtcc agttcaacat cgtggattcg aagatgctgc
     6361 gagacgccca ggatcatccg cagaactatc gcgacctgat ggtccgtgtc gccggcttct
     6421 cagcctactg gaacgagctc ggcaaaccca tccaggacga ggtcattgct cgtaccgagt
     6481 acgacgctct ctagctcgag caccaccacc accaccactg agatccggct gctaacaaag
     6541 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     6601 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
     6661 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6721 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6781 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6841 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6901 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6961 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     7021 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     7081 aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcaggtg gcacttttcg
     7141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7201 gctcatgaat taattcttag aaaaactcat cgagcatcaa atgaaactgc aatttattca
     7261 tatcaggatt atcaatacca tatttttgaa aaagccgttt ctgtaatgaa ggagaaaact
     7321 caccgaggca gttccatagg atggcaagat cctggtatcg gtctgcgatt ccgactcgtc
     7381 caacatcaat acaacctatt aatttcccct cgtcaaaaat aaggttatca agtgagaaat
     7441 caccatgagt gacgactgaa tccggtgaga atggcaaaag tttatgcatt tctttccaga
     7501 cttgttcaac aggccagcca ttacgctcgt catcaaaatc actcgcatca accaaaccgt
     7561 tattcattcg tgattgcgcc tgagcgagac gaaatacgcg atcgctgtta aaaggacaat
     7621 tacaaacagg aatcgaatgc aaccggcgca ggaacactgc cagcgcatca acaatatttt
     7681 cacctgaatc aggatattct tctaatacct ggaatgctgt tttcccgggg atcgcagtgg
     7741 tgagtaacca tgcatcatca ggagtacgga taaaatgctt gatggtcgga agaggcataa
     7801 attccgtcag ccagtttagt ctgaccatct catctgtaac atcattggca acgctacctt
     7861 tgccatgttt cagaaacaac tctggcgcat cgggcttccc atacaatcga tagattgtcg
     7921 cacctgattg
//
