LOCUS       Exported                6241 bp ds-DNA     circular SYN 12-SEP-2024
DEFINITION  synthetic circular DNA
ACCESSION   urn.local...1x-i7706kz
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 13, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Bacterial expression vector with T7lac promoter, adds N-terminal His
            tag, thrombin cleavage site, internal T7 epitope tag, N-terminal His
            tag
             kanamycin resistance
             restriction enzyme        cloning. Cut with NdeI
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    1..17
                     /standard_name="T7 promoter"
                     /label=T7 promoter
     misc_feature    20..47
                     /standard_name="lacO"
                     /label=lacO
     misc_feature    20..44
                     /standard_name="lac operator"
                     /label=lac operator
     CDS             89..1096
                     /codon_start=1
                     /standard_name="Translation 89-1096"
                     /label=Translation 89-1096
                     /translation="MGSSHHHHHHSSGLVPRGSHMDGKGKELRAVIFDVQSFSTHDGPG
                     IRTNIFFKGCALRCPWCANPESQKFSPQLLYTKMKCIGCMCCARACPHGAVTAYTAPED
                     IERYGHVRYDRGKCDKCTTHECVDACFQEALAVSGKEMTVDDVMDKIRRDSPVFRGKGG
                     VTVSGGDPLLYPEFLAELLGRCRDEGYSVALESELCVPTRNLETVMPFVSYYLTDCKII
                     DSAEHRRITGVDNEIILRNLRLIGDVCPERMCLRIPIIPGYTDSDENVGGIAAFAASCQ
                     FTKINILPYHKLGVPKHERLGTVYQLPHVQPPDDGHMRHLADVIEAHGIECVIN"
     misc_feature    101..118
                     /standard_name="6xHis Tag"
                     /label=6xHis Tag
     misc_feature    149..1096
                     /standard_name="O.uli IAD AE"
                     /label=O.uli IAD AE
     CDS             149..1096
                     /codon_start=1
                     /standard_name="Translation 149-1096"
                     /label=Translation 149-1096
                     /translation="MDGKGKELRAVIFDVQSFSTHDGPGIRTNIFFKGCALRCPWCANP
                     ESQKFSPQLLYTKMKCIGCMCCARACPHGAVTAYTAPEDIERYGHVRYDRGKCDKCTTH
                     ECVDACFQEALAVSGKEMTVDDVMDKIRRDSPVFRGKGGVTVSGGDPLLYPEFLAELLG
                     RCRDEGYSVALESELCVPTRNLETVMPFVSYYLTDCKIIDSAEHRRITGVDNEIILRNL
                     RLIGDVCPERMCLRIPIIPGYTDSDENVGGIAAFAASCQFTKINILPYHKLGVPKHERL
                     GTVYQLPHVQPPDDGHMRHLADVIEAHGIECVIN"
     misc_feature    1103..1120
                     /standard_name="6xHis Tag"
                     /label=6xHis Tag
     misc_feature    1187..1233
                     /standard_name="T7 terminator"
                     /label=T7 terminator
     misc_feature    complement(1819..2634)
                     /standard_name="KanR"
                     /label=KanR
     misc_feature    2680..3362
                     /standard_name="ColE1 origin"
                     /label=ColE1 origin
     misc_feature    2740..3359
                     /standard_name="pBR322_origin"
                     /label=pBR322_origin
     misc_feature    complement(4774..5865)
                     /standard_name="lacI"
                     /label=lacI
ORIGIN
        1 ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt
       61 ttgtttaact ttaagaagga gatataccat gggcagcagc catcatcatc atcatcacag
      121 cagcggcctg gtgccgcgcg gcagccatat ggacggcaag ggcaaagagc tcagggccgt
      181 gattttcgac gtgcagagct tttctacgca tgatggacct gggatcagga cgaacatctt
      241 cttcaagggg tgtgcgctca ggtgcccttg gtgcgccaac cccgagtcgc agaagttcag
      301 cccgcagctc ctttacacaa agatgaagtg catagggtgc atgtgctgtg ccagggcatg
      361 cccccatggg gccgtgacgg cctatactgc gccagaggac atcgagcgtt atggacacgt
      421 acggtatgac cgtggcaagt gtgataagtg caccacgcat gagtgcgtgg acgcatgctt
      481 ccaggaagcc ctcgccgtct ccgggaagga aatgaccgtc gatgacgtaa tggataagat
      541 tcgtcgcgac tctcctgtct tccggggtaa gggaggcgtc accgtatccg gtggggatcc
      601 gctgctctat cctgagttct tggccgagct gcttggtagg tgccgagatg aggggtatag
      661 cgttgccctt gagtccgagc tctgcgttcc cacgaggaac ctcgagacgg tcatgccgtt
      721 cgtaagctac taccttaccg actgcaaaat tatcgactcc gccgagcacc gcagaatcac
      781 tggtgtggac aatgagatca tcctcaggaa cctgcgcctg attggcgatg tatgcccaga
      841 gcgcatgtgc ctgcgcatac cgatcatacc tgggtacaca gactccgacg agaacgttgg
      901 cggtatcgcc gcgtttgccg cgtcgtgtca attcacaaag ataaacatcc tgccgtatca
      961 caagcttggc gtgcccaagc acgagcgcct tggcaccgtc tatcagctac ctcatgtcca
     1021 gccacccgac gatgggcata tgaggcactt ggctgacgtc atagaagccc atgggatcga
     1081 gtgcgttatc aactgactcg agcaccacca ccaccaccac tgagatccgg ctgctaacaa
     1141 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     1201 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt
     1261 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     1321 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     1381 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     1441 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     1501 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1561 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1621 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1681 caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg tggcactttt
     1741 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1801 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     1861 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     1921 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     1981 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     2041 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     2101 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     2161 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
     2221 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     2281 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     2341 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     2401 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     2461 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     2521 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     2581 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     2641 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     2701 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     2761 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     2821 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     2881 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     2941 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     3001 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     3061 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     3121 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     3181 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     3241 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     3301 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     3361 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     3421 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     3481 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     3541 attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac
     3601 aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg
     3661 gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg
     3721 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg
     3781 ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg
     3841 tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga
     3901 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg
     3961 gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa
     4021 cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt
     4081 tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt
     4141 caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct
     4201 gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac
     4261 gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag
     4321 cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc
     4381 cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc
     4441 atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag
     4501 gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg
     4561 ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg
     4621 agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac
     4681 cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa
     4741 tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     4801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     4861 gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat tgcccttcac
     4921 cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa
     4981 atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg tatcgtcgta
     5041 tcccactacc gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc
     5101 gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag
     5161 catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat
     5221 cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga
     5281 gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg
     5341 ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga tgggtgtctg
     5401 gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc
     5461 atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt
     5521 gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct
     5581 ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag
     5641 ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc
     5701 cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt
     5761 cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc
     5821 atactctgcg acatcgtata acgttactgg tttcacattc accaccctga attgactctc
     5881 ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat
     5941 ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc
     6001 cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc
     6061 cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc
     6121 gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg
     6181 cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa
     6241 a
//
