LOCUS       dCas9-KRAB_gallolyticu 14302 bp ds-DNA     circular     12-SEP-2024
DEFINITION  .
KEYWORDS    "marker:part of Fwd oligo for OL_PCR, BleoR, AmpR"
FEATURES             Location/Qualifiers
     promoter        225..423
                     /label="CMV promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus (CMV) immediate early promoter"
     LTR             441..621
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    668..793
                     /label="HIV-1 Ψ"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: packaging signal of human immunodeficiency virus type 1"
     misc_feature    1286..1519
                     /label="RRE"
                     /ApEinfo_revcolor="#ff00ff"
                     /ApEinfo_fwdcolor="#ff00ff"
                     /note="note: retroviral response element"
     misc_feature    2046..2163
                     /label="cPPT/CTS"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: central polypurine tract and central termination sequence of HIV-1"
     misc_feature    2050..2065
                     /label="cPPT"
                     /ApEinfo_revcolor="#7f007f"
                     /ApEinfo_fwdcolor="#7f007f"
     protein_bind    2205..2238
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     promoter        2262..3473
                     /label="UbC promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human ubiquitin C promoter"
     misc_feature    2270..3473
                     /label="hUbC promoter"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    3474..3496
                     /label="added"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: according to sequencing"
     misc_feature    3497..3500
                     /label="added"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: according to sequencing"
     misc_feature    3501..3616
                     /label="insert from GB10 Different VP64 XbaI Sbf"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="note: note=""Fragment extracted from GB10 Different VP64 XbaI Sbf [518 nt] : (XbaI[25] / SbfI[546])"
     regulatory      3502..3511
                     /label="Kozak sequence"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: vertebrate consensus sequence for strong initiation of translation (Kozak, 1987) regulatory_class: other: "
     misc_feature    3508..3576
                     /label="3xFlag"
                     /ApEinfo_revcolor="#1ec03a"
                     /ApEinfo_fwdcolor="#1ec03a"
     CDS             3508..3585
                     /label="Translation 3508-3585"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKHVN"
     CDS             3508..3621
                     /label="Translation 3508-3621"
                     /translation="MDYKDHDGDYKDHDIDYKDDDDKHVNPKKKRKVGRGTG"
     CDS             3511..3576
                     /label="3xFLAG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: three tandem FLAG® epitope tags, followed by an enterokinase cleavage site transl_table: 1 translation: DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    3553..3582
                     /label="HR for Gibson"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    3580..3585
                     /label="HpaI"
                     /ApEinfo_revcolor="#c0ad14"
                     /ApEinfo_fwdcolor="#c0ad14"
     misc_feature    3583..3612
                     /label="HR for Gibson"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     CDS             3586..3606
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: product: nuclear localization signal of SV40 (simian virus 40) large T antigen protein_id: transl_table: 1 translation: PKKKRKV"
     misc_feature    3586..3609
                     /label="SV40 NLS1"
                     /ApEinfo_revcolor="#008000"
                     /ApEinfo_fwdcolor="#008000"
     misc_feature    3586..3621
                     /label="cloning Fwd"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     CDS             3586..7070
                     /label="Translation 3586-7070"
                     /translation="PKKKRKVGRGTGMTNGKILGLAIGIASVGVGIIEAKTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYEIVTDFRNLNLNPYELRVKGLTEQLTNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDENRRLLKTKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILETQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILIGKCSFYPDEYRASKASYTAQEYNFLNDLNNLKVPTETGKLSTEQKEALVEFAKSTATLGPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLDSVNIDDLSREVLDKLADILTLNTEREGIEDAIRHNLPNQFTEGQISEIIKVRKSQSTAFNKGWHSFSAKLMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVRQTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLYNGTDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPIPIQELVHNSNNFEIDAILPLSLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKRDYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKISVIRGQFTSQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGNNQVVDKQTGEILSVSDDEYKELVFQPPYQGFVNMISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRKAKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDPLTWENVIEVILRDYPTTKKSEDGKNDVKCNPFEEYRRENGLVCKYSKKGKGTPIKSLKYYDKKLGNCIDITPEGSKNEVVLQSLNPWRADVYFNPETLKYELLGLKYSDLSFEKGTGKYHISQEKYDVIKEKEGIGKKSEFKFTLYRNDLILIKDTASGEQEIYRFLSRTMPNVKHYAELKPYDKEKFDNVQELVEALGEADKVGRCIKGLNKSNLSIYKVRTDVLGNKYFVKKEGDKPKLDFKNNKKTGPKKKRKVASMDAKSLTA"
     CDS             3616..3616
                     /label="dCas9 (G. Church)"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
                     /note="codon_start: 1 transl_table: 1 translation: "
     CDS             3616..3616
                     /label="dCas9"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: RNA-guided DNA-binding protein that lacks endonuclease activity due to the D10A mutation in the RuvC catalytic domain and the H840A mutation in the HNH catalytic domain product: catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system protein_id: transl_table: 1 translation: "
     CDS             3622..7011
                     /label="Translation 3622-7011"
                     /translation="MTNGKILGLAIGIASVGVGIIEAKTGKVVHANSRLFSAANAENNAERRGFRGSRRLNRRKKHRVKRVRDLFEKYEIVTDFRNLNLNPYELRVKGLTEQLTNEELFAALRTISKRRGISYLDDAEDDSTGSTDYAKSIDENRRLLKTKTPGQIQLERLEKYGQLRGNFTVYDENGEAHRLINVFSTSDYEKEARKILETQADYNKKITAEFIDDYVEILTQKRKYYHGPGNEKSRTDYGRFRTDGTTLENIFGILIGKCSFYPDEYRASKASYTAQEYNFLNDLNNLKVPTETGKLSTEQKEALVEFAKSTATLGPAKLLKEIAKILDCKVDEIKGYREDDKGKPDLHTFEPYRKLKFNLDSVNIDDLSREVLDKLADILTLNTEREGIEDAIRHNLPNQFTEGQISEIIKVRKSQSTAFNKGWHSFSAKLMNELIPELYATSDEQMTILTRLEKFKVNKKSSKNTKTIDEKEVTDEIYNPVVAKSVRQTIKIINAAVKKYGDFDKIVIEMPRDKNADDEKKFIDKRNKENKKEKDDALKRAAYLYNGTDKLPDEVFHGNKQLETKIRLWYQQGERCLYSGKPIPIQELVHNSNNFEIDAILPLSLSFDDSLANKVLVYAWTNQEKGQKTPYQVIDSMDAAWSFREMKDYVLKQKGLGKKKRDYLLTTENIDKIEVKKKFIERNLVDTRYASRVVLNSLQSALRELGKDTKISVIRGQFTSQLRRKWKIDKSRETYHHHAVDALIIAASSQLKLWEKQDNPMFVDYGNNQVVDKQTGEILSVSDDEYKELVFQPPYQGFVNMISSKGFEDEILFSYQVDSKYNRKVSDATIYSTRKAKIGKDKKEETYVLGKIKDIYSQNGFDTFIKKYNKDKTQFLMYQKDPLTWENVIEVILRDYPTTKKSEDGKNDVKCNPFEEYRRENGLVCKYSKKGKGTPIKSLKYYDKKLGNCIDITPEGSKNEVVLQSLNPWRADVYFNPETLKYELLGLKYSDLSFEKGTGKYHISQEKYDVIKEKEGIGKKSEFKFTLYRNDLILIKDTASGEQEIYRFLSRTMPNVKHYAELKPYDKEKFDNVQELVEALGEADKVGRCIKGLNKSNLSIYKVRTDVLGNKYFVKKEGDKPKLDFKNNKK"
     misc_feature    7012..7044
                     /label="gibson homology"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
     misc_feature    7018..7020
                     /label="alt. codon"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: made in DFR019 (when using primer pDR047 to PCR off of pNI141)"
     misc_feature    7018..7020
                     /label="alt. codon "
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: made in DFR019 (when using primer pDR047 to PCR off of pNI141)"
     misc_feature    7018..7038
                     /label="NLS"
                     /ApEinfo_revcolor="#c0060f"
                     /ApEinfo_fwdcolor="#c0060f"
                     /note="note: note=""direct strand translation""translation=""PKKKRKV"""
     CDS             7018..7038
                     /label="SV40 NLS"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 locus_tag: note: product: nuclear localization signal of SV40 (simian virus 40) large T antigen protein_id: transl_table: 1 translation: PKKKRKV"
     misc_feature    7018..7039
                     /label="HR for Gibson"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     misc_feature    7024..7026
                     /label="alt. codon"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: made in DFR019 (when using primer pDR047 to PCR off of pNI141)"
     misc_feature    7028..7030
                     /label="alt. codon"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: made in DFR019 (when using primer pDR047 to PCR off of pNI141)"
     misc_feature    7033..7035
                     /label="alt. codon"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: made in DFR019 (when using primer pDR047 to PCR off of pNI141)"
     misc_feature    7044..7044
                     /label="part of Fwd oligo for OL_PCR"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     CDS             7045..7074
                     /label="KRAB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: ZNF10 gene_synonym: KOX1 note: product: Krüppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)Seems that aa 11-55 is enough for full repression (Margolin et al., 1994).Note that: substitution in the A box at the DV (to AA), EEW (to AAA), and MLE (to KKK) sequences significantly inhibited the ability of GAL4-KOX1-(1-90) to repress transcription. The leucine residue in the MLE sequence occurs in the context of the highly conserved VMLENY motif common to almost all KRAB A boxes (Fig.1) and is one of the heptad repeats of leucines (MX6LX6LX6L, where X is any amino acid) capable of forming an amphipathic helix. This sequence may form a potential protein-protein interaction surface important for repression of transcription. Thus, we have identified discrete mutations in the KRAB domain that inactivate the transcriptional repression. protein_id: transl_table: 1 translation: MDAKSLTAWS,RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEP"
     CDS             7075..7260
                     /label="KRAB"
                     /ApEinfo_revcolor="#993366"
                     /ApEinfo_fwdcolor="#993366"
                     /note="codon_start: 1 gene: ZNF10 gene_synonym: KOX1 note: product: Krüppel-associated box (KRAB) transcriptional repression domain from the human zinc finger protein ZNF10 (Margolin et al., 1994)Seems that aa 11-55 is enough for full repression (Margolin et al., 1994).Note that: substitution in the A box at the DV (to AA), EEW (to AAA), and MLE (to KKK) sequences significantly inhibited the ability of GAL4-KOX1-(1-90) to repress transcription. The leucine residue in the MLE sequence occurs in the context of the highly conserved VMLENY motif common to almost all KRAB A boxes (Fig.1) and is one of the heptad repeats of leucines (MX6LX6LX6L, where X is any amino acid) capable of forming an amphipathic helix. This sequence may form a potential protein-protein interaction surface important for repression of transcription. Thus, we have identified discrete mutations in the KRAB domain that inactivate the transcriptional repression. protein_id: transl_table: 1 translation: MDAKSLTAWS,RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGYQLTKPDVILRLEKGEEP"
     misc_feature    7266..7291
                     /label="HR for Gibson"
                     /ApEinfo_revcolor="#3366ff"
                     /ApEinfo_fwdcolor="#3366ff"
     CDS             7267..7320
                     /label="T2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from Thosea asigna virus capsid protein protein_id: transl_table: 1 translation: EGRGSLLTCGDVEENPGP"
     CDS             7327..7329
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced GFP transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             7330..7332
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced GFP transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             7333..8043
                     /label="EGFP"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
                     /note="codon_start: 1 note: mammalian codon-optimized product: enhanced GFP transl_table: 1 translation: M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     CDS             8053..8109
                     /label="P2A"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 note: Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides. product: 2A peptide from porcine teschovirus-1 polyprotein transl_table: 1 translation: ATNFSLLKQAGDVEENPGP"
     CDS             8110..8531
                     /label="Bsr"
                     /ApEinfo_revcolor="#800080"
                     /ApEinfo_fwdcolor="#800080"
                     /note="codon_start: 1 transl_table: 1 translation: KTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEIISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGMCRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN*"
     protein_bind    8542..8575
                     /label="loxP"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: Cre recombinase note: Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)."
     misc_feature    8600..9188
                     /label="WPRE"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: woodchuck hepatitis virus posttranscriptional regulatory element"
     CDS             complement(9071..9082)
                     /label="Factor Xa site"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
                     /note="codon_start: 1 product: Factor Xa recognition and cleavage site transl_table: 1 translation: IEGR"
     primer_bind     complement(9191..9207)
                     /label="KS primer"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     LTR             9713..9893
                     /label="5' LTR (truncated)"
                     /ApEinfo_revcolor="#f8d3a9"
                     /ApEinfo_fwdcolor="#f8d3a9"
                     /note="note: truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    9925..10149
                     /label="bGH poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: bovine growth hormone polyadenylation signal"
     rep_origin      10195..10623
                     /label="f1 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: RIGHT note: f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis"
     promoter        10637..10966
                     /label="SV40 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: SV40 enhancer and early promoter"
     rep_origin      10817..10952
                     /label="SV40 ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="note: SV40 origin of replication"
     promoter        11014..11061
                     /label="EM7 promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: synthetic bacterial promoter "
     CDS             11080..11454
                     /label="BleoR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: Sh ble from Streptoalloteichus hindustanus note: confers resistance to bleomycin, phleomycin, and Zeocin™ product: antibiotic-binding protein transl_table: 1 translation: MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREFALRDPAGNCVHFVAEEQD*"
     polyA_signal    11584..11705
                     /label="SV40 poly(A) signal"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
                     /note="note: SV40 polyadenylation signal"
     primer_bind     complement(11754..11770)
                     /label="M13 rev"
                     /ApEinfo_revcolor="#a020f0"
                     /ApEinfo_fwdcolor="#a020f0"
                     /note="note: common sequencing primer, one of multiple similar variants"
     protein_bind    11778..11794
                     /label="lac operator"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: lac repressor encoded by lacI note: The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(11802..11808)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(11809..11826)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     promoter        complement(11827..11832)
                     /label="lac promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: promoter for the E. coli lac operon"
     protein_bind    11847..11868
                     /label="CAP binding site"
                     /ApEinfo_revcolor="#31849b"
                     /ApEinfo_fwdcolor="#31849b"
                     /note="bound_moiety: E. coli catabolite activator protein note: CAP binding activates transcription in the presence of cAMP."
     rep_origin      complement(12156..12744)
                     /label="ori"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
                     /note="direction: LEFT note: high-copy-number ColE1/pMB1/pBR322/pUC origin of replication"
     CDS             complement(12915..13706)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     CDS             complement(13707..13775)
                     /label="AmpR"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
                     /note="codon_start: 1 gene: bla note: confers resistance to ampicillin, carbenicillin, and related antibiotics product: β-lactamase transl_table: 1 translation: MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"
     promoter        complement(13776..13880)
                     /label="AmpR promoter"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="gene: bla"
     enhancer        14146..223
                     /label="CMV enhancer"
                     /ApEinfo_revcolor="#c6c9d1"
                     /ApEinfo_fwdcolor="#c6c9d1"
                     /note="note: human cytomegalovirus immediate early enhancer"
ORIGIN
        1 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
       61 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT
      121 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC
      181 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA
      241 GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT
      301 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA
      361 CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
      421 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT
      481 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG
      541 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT
      601 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC
      661 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG
      721 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG
      781 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT
      841 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT
      901 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT
      961 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC
     1021 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT
     1081 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCTGA
     1141 TCTTCAGACC TGGAGGAGGA GATATGAGGG ACAATTGGAG AAGTGAATTA TATAAATATA
     1201 AAGTAGTAAA AATTGAACCA TTAGGAGTAG CACCCACCAA GGCAAAGAGA AGAGTGGTGC
     1261 AGAGAGAAAA AAGAGCAGTG GGAATAGGAG CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG
     1321 GAAGCACTAT GGGCGCAGCG TCAATGACGC TGACGGTACA GGCCAGACAA TTATTGTCTG
     1381 GTATAGTGCA GCAGCAGAAC AATTTGCTGA GGGCTATTGA GGCGCAACAG CATCTGTTGC
     1441 AACTCACAGT CTGGGGCATC AAGCAGCTCC AGGCAAGAAT CCTGGCTGTG GAAAGATACC
     1501 TAAAGGATCA ACAGCTCCTG GGGATTTGGG GTTGCTCTGG AAAACTCATT TGCACCACTG
     1561 CTGTGCCTTG GAATGCTAGT TGGAGTAATA AATCTCTGGA ACAGATTTGG AATCACACGA
     1621 CCTGGATGGA GTGGGACAGA GAAATTAACA ATTACACAAG CTTAATACAC TCCTTAATTG
     1681 AAGAATCGCA AAACCAGCAA GAAAAGAATG AACAAGAATT ATTGGAATTA GATAAATGGG
     1741 CAAGTTTGTG GAATTGGTTT AACATAACAA ATTGGCTGTG GTATATAAAA TTATTCATAA
     1801 TGATAGTAGG AGGCTTGGTA GGTTTAAGAA TAGTTTTTGC TGTACTTTCT ATAGTGAATA
     1861 GAGTTAGGCA GGGATATTCA CCATTATCGT TTCAGACCCA CCTCCCAACC CCGAGGGGAC
     1921 CCGACAGGCC CGAAGGAATA GAAGAAGAAG GTGGAGAGAG AGACAGAGAC AGATCCATTC
     1981 GATTAGTGAA CGGATCGGCA CTGCGTGCGC CAATTCTGCA GACAAATGGC AGTATTCATC
     2041 CACAATTTTA AAAGAAAAGG GGGGATTGGG GGGTACAGTG CAGGGGAAAG AATAGTAGAC
     2101 ATAATAGCAA CAGACATACA AACTAAAGAA TTACAAAAAC AAATTACAAA AATTCAAAAT
     2161 TTTCGGGTTT ATTACAGGGA CAGCAGAGAT CCAGTTTGGT TAatATAACT TCGTATAGCA
     2221 TACATTATAC GAAGTTATta aCCCGTGTCG GCTCCAGATC TGGCCTCCGc gccgggtttt
     2281 ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag acgaagggcg
     2341 cagCgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct gctcataaga
     2401 ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact tgggtgactc
     2461 tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt cccttctcgg
     2521 cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata aggacgcgcc
     2581 gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga
     2641 tcgctgtgat cgtcacttgg tgagtAgcgg gctgctgggc tggccggggc tttcgtggcc
     2701 gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt
     2761 ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcaGcaaaat ggcggctgtt
     2821 cccgagtctt gaatggaaga cgcttgtGag gcgggctgtg aggtcgttga aacaaggtgg
     2881 ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc gggaaagctc
     2941 ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag tttgtcactg
     3001 actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatgGcggt gccgttgggc
     3061 agtgcacccg tacctttggg agcgcgcgcc Ctcgtcgtgt cgtgacgtca cccgttctgt
     3121 tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct tttctccgtc
     3181 gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg ccggacctct
     3241 ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt acctatcttc
     3301 ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt gtgttttgtg
     3361 aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat tttcagtgtt
     3421 agactagTaa attgtccgct aaattctggc cgtttttggc ttttttgtta gacGAAGCTT
     3481 GGGCTGCAGG TCGACTctag AGCCACCATG GACTACAAAG ACCATGACGG TGATTATAAA
     3541 GATCATGACA TCGATTACAA GGATGACGAT GACAAGCACG TTAACCCCAA GAAGAAGAGG
     3601 AAGGTGGGCC GCGGAACCGG TATGACAAAC GGCAAAATTC TGGGTCTGGC CATCGGAATC
     3661 GCTAGCGTTG GCGTGGGAAT CATTGAAGCG AAGACAGGTA AAGTCGTCCA TGCAAATTCT
     3721 CGATTGTTCT CCGCAGCTAA CGCTGAAAAC AATGCGGAGA GAAGGGGTTT CAGAGGCTCT
     3781 AGGCGGCTCA ACCGGCGCAA GAAGCACAGG GTAAAAAGAG TGCGAGATCT CTTTGAGAAA
     3841 TATGAGATCG TGACTGATTT TAGAAACCTG AATCTGAACC CATATGAGCT GAGAGTGAAA
     3901 GGACTTACGG AACAGCTCAC TAATGAAGAG TTGTTCGCCG CCCTGCGGAC CATCAGCAAA
     3961 CGCCGAGGAA TTTCCTACCT TGATGACGCC GAAGATGACA GTACCGGTAG CACAGATTAT
     4021 GCCAAGAGCA TTGATGAGAA CAGGAGACTG CTGAAGACTA AGACACCTGG ACAGATACAA
     4081 TTGGAACGGC TCGAGAAGTA CGGCCAGCTG AGGGGTAACT TCACCGTTTA TGACGAAAAT
     4141 GGGGAGGCCC ATAGACTGAT AAATGTGTTC TCAACTTCTG ACTATGAAAA GGAGGCCCGG
     4201 AAAATCCTCG AGACTCAAGC CGACTACAAC AAGAAGATTA CAGCCGAGTT TATTGACGAT
     4261 TACGTGGAAA TTTTAACCCA GAAAAGGAAG TATTACCACG GGCCAGGAAA TGAAAAGAGC
     4321 CGCACCGACT ATGGGAGATT CAGAACGGAT GGAACAACCT TAGAGAATAT CTTTGGAATC
     4381 CTTATTGGTA AATGCTCTTT CTATCCTGAC GAGTATCGCG CCAGCAAAGC CTCCTATACC
     4441 GCTCAGGAGT ACAACTTCTT GAATGATTTG AACAATTTGA AGGTTCCGAC GGAGACTGGC
     4501 AAGCTGAGTA CCGAGCAAAA GGAGGCCCTT GTGGAATTCG CCAAGTCTAC TGCAACATTA
     4561 GGTCCTGCTA AACTTCTGAA GGAGATTGCC AAAATTTTGG ACTGCAAAGT CGATGAAATC
     4621 AAGGGGTACC GTGAGGATGA TAAAGGGAAA CCAGACCTGC ACACCTTTGA GCCCTATAGA
     4681 AAGTTGAAAT TCAATCTGGA CAGCGTCAAC ATTGACGATT TGAGTCGCGA AGTGCTGGAC
     4741 AAGCTGGCAG ACATTTTGAC ACTTAACACT GAAAGGGAGG GCATTGAGGA TGCCATCAGG
     4801 CATAACCTGC CCAACCAATT TACTGAGGGC CAGATCTCCG AAATCATCAA GGTGCGCAAA
     4861 AGCCAGAGCA CTGCTTTCAA CAAGGGGTGG CACAGCTTCT CTGCCAAGCT CATGAACGAA
     4921 TTGATTCCCG AGCTCTATGC CACAAGCGAC GAACAGATGA CTATACTTAC TCGGCTGGAG
     4981 AAATTTAAGG TCAATAAAAA ATCCTCCAAA AACACCAAGA CGATTGACGA GAAAGAGGTC
     5041 ACTGATGAAA TCTACAATCC AGTTGTAGCC AAGTCTGTCC GGCAAACGAT CAAGATCATT
     5101 AACGCTGCTG TGAAGAAATA TGGAGACTTT GATAAGATTG TGATTGAAAT GCCTCGCGAC
     5161 AAGAATGCGG ACGATGAGAA GAAGTTTATC GATAAGAGAA ACAAAGAAAA TAAGAAAGAA
     5221 AAGGATGATG CCCTGAAGCG GGCAGCTTAC CTTTATAATG GAACCGATAA GCTGCCAGAT
     5281 GAGGTGTTTC ACGGAAACAA GCAACTTGAA ACCAAGATTC GCCTGTGGTA CCAGCAGGGA
     5341 GAACGGTGTT TGTACTCAGG CAAGCCTATC CCAATCCAGG AGTTGGTCCA CAACTCCAAT
     5401 AACTTCGAAA TCGATGCGAT TCTGCCCCTG TCCCTGAGTT TTGACGACTC CCTGGCCAAC
     5461 AAGGTGCTTG TGTATGCTTG GACCAACCAA GAGAAGGGCC AGAAGACGCC CTACCAGGTG
     5521 ATTGATTCTA TGGATGCGGC GTGGTCCTTT CGCGAGATGA AGGACTATGT GCTCAAGCAA
     5581 AAAGGCCTCG GCAAAAAGAA ACGGGATTAT CTTTTGACCA CCGAGAACAT TGACAAGATT
     5641 GAAGTGAAGA AAAAATTCAT CGAGCGCAAC TTGGTCGATA CCAGATATGC CTCTAGGGTT
     5701 GTGCTGAACT CACTGCAGTC TGCTTTGAGA GAGCTGGGTA AAGACACTAA AATTAGTGTA
     5761 ATCAGGGGCC AGTTCACAAG TCAGCTTAGG CGGAAATGGA AGATCGACAA GTCACGCGAG
     5821 ACATATCATC ATCACGCAGT CGACGCACTG ATAATTGCAG CTTCAAGTCA GCTCAAGTTG
     5881 TGGGAGAAAC AGGATAACCC TATGTTTGTC GACTATGGAA ACAATCAGGT CGTCGATAAG
     5941 CAGACCGGGG AAATTTTAAG TGTGTCCGAT GACGAGTATA AGGAGCTTGT CTTTCAGCCA
     6001 CCGTACCAGG GCTTTGTCAA CATGATTAGT AGCAAGGGTT TTGAGGACGA AATTTTGTTC
     6061 AGCTACCAGG TCGATTCCAA ATACAATAGA AAAGTATCCG ACGCAACCAT ATATTCTACT
     6121 CGCAAGGCCA AGATTGGCAA AGATAAGAAG GAAGAGACCT ATGTATTGGG GAAGATCAAA
     6181 GACATTTACT CACAAAATGG ATTCGACACC TTCATTAAGA AGTACAACAA AGATAAGACA
     6241 CAGTTTTTGA TGTACCAGAA AGATCCACTG ACATGGGAAA ACGTGATCGA AGTTATACTG
     6301 CGTGACTACC CCACGACTAA AAAGAGTGAG GACGGAAAAA ACGACGTGAA GTGCAACCCG
     6361 TTTGAAGAAT ACCGGAGAGA AAACGGTCTG GTGTGTAAGT ACTCTAAGAA AGGAAAGGGG
     6421 ACCCCTATTA AATCCCTCAA ATACTACGAC AAAAAACTCG GGAACTGCAT CGATATCACC
     6481 CCGGAAGGTT CCAAAAATGA AGTCGTGCTT CAATCCTTGA ATCCGTGGAG GGCAGATGTG
     6541 TACTTTAACC CAGAAACCTT GAAGTATGAA TTACTGGGAC TTAAATACAG TGATCTCTCA
     6601 TTTGAAAAGG GCACTGGAAA ATACCATATC TCTCAGGAGA AGTACGACGT CATTAAGGAA
     6661 AAAGAAGGGA TCGGGAAAAA ATCCGAGTTC AAGTTCACAT TGTATAGGAA CGACCTGATC
     6721 CTTATTAAAG ACACAGCCAG CGGTGAGCAG GAGATTTACC GATTTCTGTC TAGAACCATG
     6781 CCTAACGTCA AGCACTATGC GGAGCTGAAG CCCTATGACA AAGAAAAATT TGATAACGTC
     6841 CAGGAACTCG TCGAGGCGCT GGGCGAAGCC GACAAGGTAG GCCGCTGTAT AAAGGGGCTG
     6901 AACAAAAGCA ACCTCAGCAT CTATAAAGTT AGGACAGATG TGCTCGGGAA CAAATACTTC
     6961 GTTAAGAAGG AAGGGGACAA GCCCAAGCTG GATTTTAAGA ACAATAAAAA GACCGGTCCT
     7021 AAGAAAAAGC GGAAAGTGGc tagCatggat gctaagtcac taactgcctg gtccCGGACA
     7081 CTGGTGACCT TCAAGGATGT ATTTGTGGAC TTCACCAGGG AGGAGTGGAA GCTGCTGGAC
     7141 ACTGCTCAGC AGATCGTGTA CAGAAATGTG ATGCTGGAGA ACTATAAGAA CCTGGTTTCC
     7201 TTGGGTTATC AGCTTACTAA GCCAGATGTG ATCCTCCGGT TGGAGAAGGG AGAAGAGCCC
     7261 GctagCGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAGAA TCCCGGCCCT
     7321 GgacCAATGG TGAGCAAGGG CGAGGAGCTG TTCACCGGGG TGGTGCCCAT CCTGGTCGAG
     7381 CTGGACGGCG ACGTAAACGG CCACAAGTTC AGCGTGTCCG GCGAGGGCGA GGGCGATGCC
     7441 ACCTACGGCA AGCTGACCCT GAAGTTCATC TGCACCACCG GCAAGCTGCC CGTGCCCTGG
     7501 CCCACCCTCG TGACCACCCT GACCTACGGC GTGCAGTGCT TCAGCCGCTA CCCCGACCAC
     7561 ATGAAGCAGC ACGACTTCTT CAAGTCCGCC ATGCCCGAAG GCTACGTCCA GGAGCGCACC
     7621 ATCTTCTTCA AGGACGACGG CAACTACAAG ACCCGCGCCG AGGTGAAGTT CGAGGGCGAC
     7681 ACCCTGGTGA ACCGCATCGA GCTGAAGGGC ATCGACTTCA AGGAGGACGG CAACATCCTG
     7741 GGGCACAAGC TGGAGTACAA CTACAACAGC CACAACGTCT ATATCATGGC CGACAAGCAG
     7801 AAGAACGGCA TCAAGGTGAA CTTCAAGATC CGCCACAACA TCGAGGACGG CAGCGTGCAG
     7861 CTCGCCGACC ACTACCAGCA GAACACCCCC ATCGGCGACG GCCCCGTGCT GCTGCCCGAC
     7921 AACCACTACC TGAGCACCCA GTCCGCCCTG AGCAAAGACC CCAACGAGAA GCGCGATCAC
     7981 ATGGTCCTGC TGGAGTTCGT GACCGCCGCC GGGATCACTC TCGGCATGGA CGAGCTGTAC
     8041 AAGGGATCCG GCGCAACAAA CTTCTCTCTG CTGAAACAAG CCGGAGATGT CGAAGAGAAT
     8101 CCTGGACCGA AGACCTTCAA CATCTCTCAG CAGGATCTGG AGCTGGTGGA GGTCGCCACT
     8161 GAGAAGATCA CCATGCTCTA TGAGGACAAC AAGCACCATG TCGGGGCGGC CATCAGGACC
     8221 AAGACTGGGG AGATCATCTC TGCTGTCCAC ATTGAGGCCT ACATTGGCAG GGTCACTGTC
     8281 TGTGCTGAAG CCATTGCCAT TGGGTCTGCT GTGAGCAACG GGCAGAAGGA CTTTGACACC
     8341 ATTGTGGCTG TCAGGCACCC CTACTCTGAT GAGGTGGACA GATCCATCAG GGTGGTCAGC
     8401 CCCTGTGGCA TGTGCAGAGA GCTCATCTCT GACTATGCTC CTGACTGCTT TGTGCTCATT
     8461 GAGATGAATG GCAAGCTGGT CAAAACCACC ATTGAGGAAC TCATCCCCCT CAAGTACACC
     8521 AGGAACTAAA GTTGATAATA GATAACTTCG TATAGCATAC ATTATACGAA GTTATGAATT
     8581 CGATATCAAG CTTATCGATA ATCAACCTCT GGATTACAAA ATTTGTGAAA GATTGACTGG
     8641 TATTCTTAAC TATGTTGCTC CTTTTACGCT ATGTGGATAC GCTGCTTTAA TGCCTTTGTA
     8701 TCATGCTATT GCTTCCCGTA TGGCTTTCAT TTTCTCCTCC TTGTATAAAT CCTGGTTGCT
     8761 GTCTCTTTAT GAGGAGTTGT GGCCCGTTGT CAGGCAACGT GGCGTGGTGT GCACTGTGTT
     8821 TGCTGACGCA ACCCCCACTG GTTGGGGCAT TGCCACCACC TGTCAGCTCC TTTCCGGGAC
     8881 TTTCGCTTTC CCCCTCCCTA TTGCCACGGC GGAACTCATC GCCGCCTGCC TTGCCCGCTG
     8941 CTGGACAGGG GCTCGGCTGT TGGGCACTGA CAATTCCGTG GTGTTGTCGG GGAAATCATC
     9001 GTCCTTTCCT TGGCTGCTCG CCTGTGTTGC CACCTGGATT CTGCGCGGGA CGTCCTTCTG
     9061 CTACGTCCCT TCGGCCCTCA ATCCAGCGGA CCTTCCTTCC CGCGGCCTGC TGCCGGCTCT
     9121 GCGGCCTCTT CCGCGTCTTC GCCTTCGCCC TCAGACGAGT CGGATCTCCC TTTGGGCCGC
     9181 CTCCCCGCAT CGATACCGTC GACCTCGAGA CCTAGAAAAA CATGGAGCAA TCACAAGTAG
     9241 CAATACAGCA GCTACCAATG CTGATTGTGC CTGGCTAGAA GCACAAGAGG AGGAGGAGGT
     9301 GGGTTTTCCA GTCACACCTC AGGTACCTTT AAGACCAATG ACTTACAAGG CAGCTGTAGA
     9361 TCTTAGCCAC TTTTTAAAAG AAAAGGGGGG ACTGGAAGGG CTAATTCACT CCCAACGAAG
     9421 ACAAGATATC CTTGATCTGT GGATCTACCA CACACAAGGC TACTTCCCTG ATTGGCAGAA
     9481 CTACACACCA GGGCCAGGGA TCAGATATCC ACTGACCTTT GGATGGTGCT ACAAGCTAGT
     9541 ACCAGTTGAG CAAGAGAAGG TAGAAGAAGC CAATGAAGGA GAGAACACCC GCTTGTTACA
     9601 CCCTGTGAGC CTGCATGGGA TGGATGACCC GGAGAGAGAA GTATTAGAGT GGAGGTTTGA
     9661 CAGCCGCCTA GCATTTCATC ACATGGCCCG AGAGCTGCAT CCGGACTGTA CTGGGTCTCT
     9721 CTGGTTAGAC CAGATCTGAG CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA
     9781 GCCTCAATAA AGCTTGCCTT GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC
     9841 TGGTAACTAG AGATCCCTCA GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGGGCCCG
     9901 TTTAAACCCG CTGATCAGCC TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC
     9961 CCTCCCCCGT GCCTTCCTTG ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA
    10021 ATGAGGAAAT TGCATCGCAT TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG
    10081 GGCAGGACAG CAAGGGGGAG GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG
    10141 GCTCTATGGC TTCTGAGGCG GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC
    10201 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC
    10261 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG
    10321 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT
    10381 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC
    10441 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT
    10501 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA
    10561 TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA
    10621 ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG
    10681 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG
    10741 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC
    10801 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA
    10861 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT
    10921 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC
    10981 TTGTATATCC ATTTTCGGAT CTGATCAGCA CGTGTTGACA ATTAATCATC GGCATAGTAT
    11041 ATCGGCATAG TATAATACGA CAAGGTGAGG AACTAAACCA TGGCCAAGTT GACCAGTGCC
    11101 GTTCCGGTGC TCACCGCGCG CGACGTCGCC GGAGCGGTCG AGTTCTGGAC CGACCGGCTC
    11161 GGGTTCTCCC GGGACTTCGT GGAGGACGAC TTCGCCGGTG TGGTCCGGGA CGACGTGACC
    11221 CTGTTCATCA GCGCGGTCCA GGACCAGGTG GTGCCGGACA ACACCCTGGC CTGGGTGTGG
    11281 GTGCGCGGCC TGGACGAGCT GTACGCCGAG TGGTCGGAGG TCGTGTCCAC GAACTTCCGG
    11341 GACGCCTCCG GGCCGGCCAT GACCGAGATC GGCGAGCAGC CGTGGGGGCG GGAGTTCGCC
    11401 CTGCGCGACC CGGCCGGCAA CTGCGTGCAC TTCGTGGCCG AGGAGCAGGA CTGACACGTG
    11461 CTACGAGATT TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC
    11521 CGGGACGCCG GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC
    11581 CCCAACTTGT TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC
    11641 ACAAATAAAG CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA
    11701 TCTTATCATG TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG
    11761 CTGTTTCCTG TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC
    11821 ATAAAGTGTA AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC
    11881 TCACTGCCCG CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA
    11941 CGCGCGGGGA GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG
    12001 CTGCGCTCGG TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG
    12061 TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG
    12121 GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC
    12181 GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA
    12241 TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT
    12301 ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC
    12361 TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC
    12421 CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA
    12481 AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT
    12541 GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA
    12601 GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT
    12661 TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT
    12721 ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT
    12781 CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC
    12841 ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA
    12901 ACTTGGTCTG ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA
    12961 TTTCGTTCAT CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC
    13021 TTACCATCTG GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT
    13081 TTATCAGCAA TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA
    13141 TCCGCCTCCA TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT
    13201 AATAGTTTGC GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT
    13261 GGTATGGCTT CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG
    13321 TTGTGCAAAA AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC
    13381 GCAGTGTTAT CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC
    13441 GTAAGATGCT TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG
    13501 CGGCGACCGA GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA
    13561 ACTTTAAAAG TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA
    13621 CCGCTGTTGA GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT
    13681 TTTACTTTCA CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG
    13741 GGAATAAGGG CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA
    13801 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT
    13861 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG
    13921 GGAGATCTCC CGATCCCCTA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT
    13981 TAAGCCAGTA TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA
    14041 TTTAAGCTAC AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGGGTTA
    14101 GGCGTTTTGC GCTGCTTCGC GATGTACGGG CCAGATATAC GCGTTGACAT TGATTATTGA
    14161 CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT ATGGAGTTCC
    14221 GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC CCCCGCCCAT
    14281 TGACGTCAAT AATGACGTAT GT
//