LOCUS       Exported                6944 bp ds-DNA     circular SYN 01-AUG-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pAAV_AR-RE_MLP_BC1011-luc2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Sep 17, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     LOCUS       pAAVspace_DEST_luc2_WPRE      8137 bp    DNA   CIRCULAR 
                  07-01-2013

            DEFINITION  Gateway Parent Destination Vector, AAV destination 
            vector with firefly luciferase ORF

            ACCESSION   V825

            KEYWORDS    

            SOURCE      

              ORGANISM  

            REFERENCE   1 - 8137 (bases 1 to 8137)

              AUTHORS   

              JOURNAL   

            COMMENT
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          259..1065
                     /label=seq verified pAAV
                     /note="seq verified pAAV"
     source          1053..1056
                     /label=AGCT inserted
                     /note="AGCT inserted"
     misc_feature    0
                     /label=Untitled
     misc_feature    0
                     /label=Untitled
     misc_feature    1..183
                     /label=ITR
                     /note="ITR"
     protein_bind    484..508
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     enhancer        509..568
                     /label=4x AR-RE
                     /note="4x AR-RE"
     enhancer        510..520
                     /label=AR-GRE
                     /note="AR-GRE"
     enhancer        525..535
                     /label=AR-GRE
                     /note="AR-GRE"
     enhancer        540..550
                     /label=AR-GRE
                     /note="AR-GRE"
     enhancer        555..565
                     /label=AR-GRE
                     /note="AR-GRE"
     misc_feature    569..589
                     /label=spacer 1
                     /note="spacer 1"
     enhancer        590..634
                     /label=3x AR-RE
                     /note="4x AR-RE"
     enhancer        591..601
                     /label=AR-GRE
                     /note="AR-GRE"
     enhancer        606..616
                     /label=AR-GRE
                     /note="AR-GRE"
     enhancer        621..631
                     /label=AR-GRE
                     /note="AR-GRE"
     protein_bind    complement(635..655)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        706..746
                     /label=Adenovirus major late promoter
                     /note="Adenovirus major late promoter"
     protein_bind    796..816
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     817..842
                     /label=Dec1 #10243
                     /note="Dec1 #10243"
     promoter        843..862
                     /label=T3
                     /note="T3"
     promoter        843..861
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        863..875
                     /label=truncated SP6
                     /note="truncated SP6"
     misc_feature    876..924
                     /label=E01011
                     /note="E01011"
     promoter        complement(925..943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(944..969)
                     /label=Dec2 #9752
                     /note="Dec2 #9752"
     protein_bind    complement(967..991)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      1070..1079
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1076..2728
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTITEKEIVDYVASQATTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     misc_feature    1186
                     /label=modified base, silent mutation, C--&gt;T
                     /note="modified base, silent mutation, C-->T"
     misc_feature    2554
                     /label=modified base
                     /note="modified base, point mutation, atg --> atA (Met --> 
                     Ile)"
     misc_feature    2592
                     /label=modified base
                     /note="modified base, point mutation, gtt --> gCt (Val --> 
                     Ala)"
     misc_feature    2750..3342
                     /label=WPRE
                     /note="WPRE"
     misc_feature    2756..3344
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3227..3238)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    3361..3629
                     /label=bGH_PA_terminator
                     /note="bGH_PA_terminator"
     polyA_signal    3377..3601
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    3639..3821
                     /label=ITR
                     /note="ITR"
     promoter        complement(3822..4151)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3836..3971
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(4338..4971)
                     /direction=LEFT
                     /label=pUC
                     /note="pUC"
     rep_origin      complement(4397..4985)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5156..6016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6017..6121)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6909..6927
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
        1 tagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggtacc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
      241 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
      301 catcctgggg cacaagctgg agtacaacta caacagccac aacgtccata tcatggccga
      361 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
      421 cgtgcagctc gccgaccact catatgtact cgagttgtcg acggtatcga taagcttgat
      481 atcacaagtt tgtacaaaaa agcaggctag aacatattgt tctagaacat attgttctag
      541 aacatattgt tctagaacat attgttctac gcgtggcctc gcctgcagga gaacatattg
      601 ttctagaaca tattgttcta gaacatattg ttctcaactt ttctatacaa agttggcaag
      661 tgcaggtgcc agaacatttc tctggcctaa ctggaagacg ctagcggggg gctataaaag
      721 ggggtggggg cgttcgtcct cactctagat ctgcgatcta agtaagcttg gcaatccggt
      781 actgttggta aagcccaact ttgtataata aagttgagct agttgctaag tctgccgagt
      841 agaattaacc ctcactaaag ggtaggtgac actattctaa catcttttct aacattgtgg
      901 gtgtacattc tatctattac ttaccctata gtgagtcgta ttagtagacg cgagtcaatg
      961 catgtacgac agctttcttg tacaaagtgg tgatatcgaa ttcctgcagc ccgggggatc
     1021 cgcccgggct agagcggccg ccaccgcggt ggagctcgcg actagtgacg ccaccatgga
     1081 agatgccaaa aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc
     1141 cggcgagcag ctgcacaaag ccatgaagcg ctacgccctg gtgcctggca ccatcgcctt
     1201 taccgacgca catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg
     1261 gctggcagaa gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag
     1321 cgagaatagc ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt
     1381 ggccccagct aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca
     1441 gcccaccgtc gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa
     1501 gctaccgatc atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca
     1561 aagcatgtac accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt
     1621 gcccgagagc ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac
     1681 cggattgccc aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc
     1741 ccgcgacccc atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc
     1801 atttcaccac ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt
     1861 cgtgctcatg taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat
     1921 tcaatctgcc ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga
     1981 caagtacgac ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga
     2041 ggtaggtgag gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct
     2101 gacagaaaca accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt
     2161 aggcaaggtg gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact
     2221 gggtgtgaac cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt
     2281 taacaacccc gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga
     2341 catcgcctac tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat
     2401 caaatacaag ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc
     2461 caacatcttc gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc
     2521 cgcagtcgtc gtgctggaac acggtaaaac cataaccgag aaggagatcg tggactatgt
     2581 ggccagccag gctacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt
     2641 gcctaaagga ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc
     2701 caagaagggc ggcaagatcg ccgtgtaatt cgaattcgat atcaagctta tcgataatca
     2761 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2821 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2881 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2941 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     3001 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     3061 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     3121 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     3181 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     3241 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     3301 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgact
     3361 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3421 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3481 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3541 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3601 gcttctgagg cggaaagaac cagctggggc tcgactagag catggctacg tagataagta
     3661 gcatggcggg ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc
     3721 tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt
     3781 tgcccgggcg gcctcagtga gcgagcgagc gcgcagagct ttttgcaaaa gcctaggcct
     3841 ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc ctcggcctct
     3901 gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact gggcggagtt
     3961 aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt gagatgcatg
     4021 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt tgctgactaa
     4081 ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt tccacaccct
     4141 aactgacaca cattccacag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     4201 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     4261 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     4321 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     4381 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     4441 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     4501 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     4561 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     4621 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     4681 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     4741 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     4801 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     4861 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     4921 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     4981 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     5041 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     5101 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     5161 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     5221 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     5281 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     5341 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     5401 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     5461 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     5521 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     5581 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     5641 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     5701 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     5761 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     5821 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     5881 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     5941 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     6001 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     6061 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     6121 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     6181 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     6241 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     6301 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     6361 actatgcggc atcagagcag attgtactga gagtgcacca ttcgacgctc tcccttatgc
     6421 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     6481 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     6541 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     6601 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     6661 atgcgtccgg cgtagaggat ctggctagcg atgaccctgc tgattggttc gctgaccatt
     6721 tccgggtgcg ggacggcgtt accagaaact cagaaggttc gtccaaccaa accgactctg
     6781 acggcagttt acgagagaga tgatagggtc tgcttcagta agccagatgc tacacaatta
     6841 ggcttgtaca tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata
     6901 cacatacgat ttaggtgaca ctatagaata cacggaatta attc
//
