LOCUS       pAAV-hSyn1-DIO-SEP-GluA 7464 bp ds-DNA     circular     02-OCT-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label="ITR 5'"
     ITR element     12..141
                     /label="Flop"
     ITR element     complement(38..46)
                     /label="ITR-C"
     Should avoid    complement(46..50)
                     /label="rep-binding seq"
     ITR element     50..58
                     /label="ITR-C"
     ITR element     complement(60..68)
                     /label="ITR-B"
     Should avoid    68..72
                     /label="rep-binding seq"
     ITR element     72..80
                     /label="ITR-B"
     ITR element     81..121
                     /label="ITR-A"
     Should avoid    complement(116..121)
                     /label="trs"
     Should avoid    118..123
                     /label="trs (AAV2)"
     ITR element     complement(121..141)
                     /label="ITR-D"
     misc_feature    149..617
                     /label="hSyn1 promoter"
     primer          complement(242..261)
                     /label="RJ1546"
                     /note="sequence: TGGGGGTTGGGTGCTTGTCC"
     primer          543..583
                     /label="RJ1853"
                     /note="sequence: TGCGGCGCCGGCGACTCAGCGCTGCCTCAGTCTGCGGTGGG"
     primer          556..576
                     /label="RJ1517"
                     /note="sequence: ACTCAGCGCTGCCTCAGTCTG"
     misc_feature    618..651
                     /label="Lox2272"
     primer          complement(674..725)
                     /label="RJ2194"
                     /note="sequence: AGGActctagATAACTTCGTATAATGTATGCTATACGAAGTTATATAGCAGC"
     misc_feature    682..715
                     /label="None"
     misc_feature    682..715
                     /label="LoxP"
     primer          706..739
                     /label="RJ2195"
                     /note="sequence: ACGAAGTTATctagagTCCTGTGGCTCCCAAGGG"
     CDS             complement(716..3379)
                     /label="GluA1 flop"
                     /translation="FPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL*"
     CDS             complement(716..3382)
                     /label="GluA1 flop rat mature"
                     /translation="NFPNNIQIGGLFPNQQSQEHAAFRFALSQLTEPPKLLPQIDIVNISDSFEMTYRFCSQFSKGVYAIFGFYERRTVNMLTSFCGALHVCFITPSFPVDTSNQFVLQLRPELQEALISIIDHYKWQTFVYIYDADRGLSVLQRVLDTAAEKNWQVTAVNILTTTEEGYRMLFQDLEKKKERLVVVDCESERLNAILGQIVKLEKNGIGYHYILANLGFMDIDLNKFKESGANVTGFQLVNYTDTIPARIMQQWRTSDSRDHTRVDWKRPKYTSALTYDGVKVMAEAFQSLRRQRIDISRRGNAGDCLANPAVPWGQGIDIQRALQQVRFEGLTGNVQFNEKGRRTNYTLHVIEMKHDGIRKIGYWNEDDKFVPAATDAQAGGDNSSVQNRTYIVTTILEDPYVMLKKNANQFEGNDRYEGYCVELAAEIAKHVGYSYRLEIVSDGKYGARDPDTKAWNGMVGELVYGRADVAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIVFAYIGVSVVLFLVSRFSPYEWHSEEFEEGRDQTTSDQSNEFGIFNSLWFSLGAFMRQGCDISPRSLSGRIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLEAGSTKEFFRRSKIAVFEKMWTYMKSAEPSVFVRTTEEGMIRVRKSKGKYAYLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGIATPKGSALRNPVNLAVLKLNEQGLLDKLKNKWWYDKGECGSGGGDSKDKTSALSLSNVAGVFYILIGGLGLAMLVALIEFCYKSRSESKRMKGFCLIPQQSINEAIRTSTLPRNSGAGASGGGGSGENGRVVSQDFPKSMQSIPCMSHSSGMPLGATGL*"
     misc_feature    1058..1168
                     /label="flop exon"
     primer          complement(1586..1633)
                     /label="RJ1774"
                     /note="sequence: tgtgaCATTTCCCCCAGGTCCCTGTCCGGACGCATCGTCGGCGGCGTC"
     primer          complement(1631..1651)
                     /label="RJ2198"
                     /note="sequence: GCCTTCATGCgGCAAGGATGT"
     primer          1633..1652
                     /label="RJ2197"
                     /note="sequence: ATCCTTGCcGCATGAAGGCC"
     misc_feature    1640..1642
                     /label="Q582R"
     primer          2019..2055
                     /label="DZ11"
                     /note="sequence: CAAGCCTTTGTGTCGGGATCCCGGGCTCCATATTTGC"
     primer          2746..2785
                     /label="RJ1768"
                     /note="sequence: TGGCGAGGATGTAGTGGTACCCGATGCCATTCTTTTCTAG"
     primer          complement(3356..3407)
                     /label="RJ2196"
                     /note="sequence: ACTATACAAAGGTGGATCTACCGGTaatTTCCCCAACAATATCCAGATAGGG"
     misc_feature    3383..4108
                     /label="None"
     misc_feature    complement(3383..4108)
                     /label="None"
     CDS             complement(3383..4108)
                     /label="Translation 4108-3383"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFVTAAGITHGMDELYKGGSTG"
     Should avoid    3397..3401
                     /label="rep-binding seq"
     CDS             complement(3398..4108)
                     /label="pHluorin superecliptic"
     primer          3423..3442
                     /label="RJ2019"
                     /note="sequence: GTAATCCCAGCAGCTGTTAC"
     primer          complement(3468..3509)
                     /label="EG19"
                     /note="sequence: GTTTACAACTTCTACTCTTTCGAAAGATCCCAACGAAAAGAG"
     primer          complement(3509..3528)
                     /label="RJ1520 (seq)"
                     /note="sequence: TACCAGACAACCATTACCTG"
     primer          3567..3589
                     /label="RJ1170"
                     /note="sequence: TAATGGTCTGCTAGTTGAACGCC"
     primer          3567..3589
                     /label="RJ1170"
                     /note="sequence: TAATGGTCTGCTAGTTGAACGCC"
     primer          complement(3567..3589)
                     /label="RJ1224"
                     /note="sequence: GGCGTTCAACTAGCAGACCATTA"
     primer          complement(3567..3589)
                     /label="RJ1220"
                     /note="sequence: GGCGTTCAACTAGCAGACCATTA"
     primer          3588..3607
                     /label="RJ775"
                     /note="sequence: CCTCCATCTTCAATGTTGTG"
     Should avoid    3625..3629
                     /label="rep-binding seq"
     primer          3648..3700
                     /label="EG43"
                     /note="sequence: TCTGCCATGATGTACACCTGGTGATCGTTATAGTTGTATTCCAATTTGTGTCC"
     primer          complement(3786..3804)
                     /label="RJ774"
                     /note="sequence: ACGGGAACTACAAGACACG"
     Should avoid    3808..3812
                     /label="rep-binding seq"
     primer          3923..3945
                     /label="RJ1528"
                     /note="sequence: AGTAGTGACAAGTGTTGGCCaaG"
     Should avoid    3937..3942
                     /label="trs"
     primer          3938..3960
                     /label="RJ1168"
                     /note="sequence: TGGCCAAGGAACAGGTAGTTTTC"
     Should avoid    complement(3939..3944)
                     /label="trs"
     primer          complement(3940..3962)
                     /label="RJ1169"
                     /note="sequence: TGGAAAACTACCTGTTCCTTGGC"
     primer          complement(3940..3962)
                     /label="RJ1169"
                     /note="sequence: TGGAAAACTACCTGTTCCtTGGC"
     primer          3949..3969
                     /label="RJ1519 (seq)"
                     /note="sequence: CAGGTAGTTTTCCAGTAGTGC"
     primer          4008..4029
                     /label="SEPNR1 gDNA (Fwd) 474-495 REV"
                     /note="sequence: CCTTCACCCTCTCCACTGACAG"
     primer          4008..4029
                     /label="RJ1526"
                     /note="sequence: CCTTCACCCTCTCCACTGACAG"
     misc_feature    4109..4183
                     /label="None"
     misc_feature    complement(4109..4183)
                     /label="GluA2 kozak + Signal Peptide"
     CDS             complement(4109..4183)
                     /label="Translation 4183-4109"
                     /translation="MQKIMHISVLLSPVLWGLIFGVSSN"
     misc_feature    4184..4226
                     /label="None"
     misc_feature    complement(4193..4226)
                     /label="Lox2272 (inv)"
     primer          4229..4252
                     /label="RJ1734"
                     /note="sequence: gccttaacccagaaattatcactg"
     misc_feature    complement(4257..4290)
                     /label="LoxP (inv)"
     misc_feature    4257..4718
                     /label="None"
     Should avoid    4388..4392
                     /label="rep-binding seq"
     primer          complement(4398..4419)
                     /label="RJ1843"
                     /note="sequence: AGCCATACGGGAAGCAATAGCA"
     primer          complement(4399..4417)
                     /label="AB11"
                     /note="sequence: CCATACGGGAAGCAATAGC"
     primer          4517..4534
                     /label="RJ1547"
                     /note="sequence: TGGGCACTGACAATTCCG"
     regulatory      4539..4718
                     /label="SL polyA signal"
     primer          4549..4568
                     /label="SV40p-AR"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     primer          4598..4617
                     /label="SV40p-AR"
                     /note="sequence: GAAATTTGTGATGCTATTGC"
     primer          complement(4630..4674)
                     /label="RJ1628"
                     /note="sequence: TGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATG"
     primer          4649..4669
                     /label="p379 pEGFP rev"
                     /note="sequence: CAAGTTAACAACAACAATTGC"
     primer          4649..4669
                     /label="(p379) pEGFP 1504-1524 r eGFPC1rev"
                     /note="sequence: CAAGTTAACAACAACAATTGC"
     primer          complement(4660..4697)
                     /label="RJ1667"
                     /note="sequence: CCCCCTGAACCTGAAACATAAAATGAATGCAATTGTTG"
     ITR element     4727..4747
                     /label="ITR-D"
     ITR element     complement(4727..4856)
                     /label="Flop"
     LTR             4728..4868
                     /label="ITR 3'"
     Should avoid    complement(4745..4750)
                     /label="trs (AAV2)"
     Should avoid    4747..4752
                     /label="trs"
     ITR element     complement(4747..4787)
                     /label="ITR-A"
     ITR element     complement(4788..4796)
                     /label="ITR-B"
     Should avoid    complement(4796..4800)
                     /label="rep-binding seq"
     ITR element     4800..4808
                     /label="ITR-B"
     ITR element     complement(4810..4818)
                     /label="ITR-C"
     Should avoid    4818..4822
                     /label="rep-binding seq"
     ITR element     4822..4830
                     /label="ITR-C"
     ITR element     4831..4871
                     /label="ITR-A variant 1"
     Should avoid    complement(4927..4931)
                     /label="rep-binding seq"
     Should avoid    5209..5213
                     /label="rep-binding seq"
     primer          5533..5555
                     /label="pGEX 3"
                     /note="sequence: CCGGGAGCTGCATGTGTCAGAGG"
     primer          complement(5586..5605)
                     /label="RJ1968"
                     /note="sequence: cgtatcacgaggccctttcg"
     primer          complement(5728..5750)
                     /label="MiniT reverse"
                     /note="sequence: TCAGGGTTATTGTCTCATGAGCG"
     gene            5784..6644
                     /label="bla"
     CDS             complement(5784..6644)
                     /label="Ampicillin"
     Should avoid    complement(5893..5898)
                     /label="trs (AAV2)"
     Should avoid    complement(6179..6184)
                     /label="trs"
     Should avoid    6181..6186
                     /label="trs (AAV2)"
     rep_origin      6789..7462
                     /label="pUC origin"
     Should avoid    complement(6803..6807)
                     /label="rep-binding seq"
     Should avoid    6906..6911
                     /label="trs (AAV2)"
     primer          7398..7415
                     /label="RJ1552"
                     /note="sequence: tggaaaaacgccagcaac"
     ITR element     complement(7461..37)
                     /label="ITR-A variant 2"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCT GCAGAGGGCC CTGCGTATGA GTGCAAGTGG
      181 GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC
      241 TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG
      301 GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT
      361 CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC
      421 TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG
      481 GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC
      541 GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT
      601 CGTGCCTGAG AGCGCAGATA ACTTCGTATA GGATACTTTA TACGAAGTTA TGCAGAATGG
      661 TAGCTGGATT GTAGCTGCTA TATAACTTCG TATAGCATAC ATTATACGAA GTTATctaga
      721 gTCCTGTGGC TCCCAAGGGC ATCCCTGAAC TGTGACTCAT GCAGGGAATG GATTGCATGG
      781 ACTTGGGGAA GTCCTGGCTG ACCACCCGGC CATTCTCTCC ACTGCCGCCT CCTCCGCTGG
      841 CTCCTGCCCC ACTGTTCCGG GGGAGGGTCG ATGTCCGTAT GGCTTCATTG ATGGATTGCT
      901 GTGGGATCAA ACAGAAACCC TTCATCCGCT TCGACTCGCT ACGGGATTTG TAGCAGAACT
      961 CGATTAAGGC AACCAGCATG GCCAGTCCCA GCCCTCCAAT CAGGATGTAG AACACGCCTG
     1021 CCACATTGCT GAGGCTCAAA GCGCTGGTCT TGTCCTTGGA GTCACCTCCC CCGCTGCCGC
     1081 ACTCGCCCTT GTCGTACCAC CATTTGTTTT TCAATTTGTC CAAAAGCCCC TGCTCGTTCA
     1141 GTTTTAACAC TGCCAGGTTT ACTGGATTTC TCAGGGCGGA CCCCTTGGGT GTCGCAATGC
     1201 CATAGCCTTT GGAATCCAAG TTACCTCCCA CTTTCATGGT GTCACAGGGC TTTCGTTGCT
     1261 CAATATACTC ATTCATGGTG GACTCCAGGA GGTAGGCGTA TTTGCCTTTA GATTTTCTCA
     1321 CTCTGATCAT GCCTTCCTCT GTGGTCCGAA CAAACACGGA TGGTTCTGCA GACTTCATGT
     1381 ATGTCCACAT CTTCTCAAAC ACAGCGATTT TAGATCTCCT GAAGAACTCC TTAGTGGAGC
     1441 CTGCTTCCAA TGTCCCATAA GCAATTTCCG TCTGCTTTGC CAGGTCCTCT GCACTCTCAA
     1501 TGGGAGACAC CATCCTCTCC ACAGTCAGGA AGGCAGCCAG GTTGGCTGTG TACGAGGAGA
     1561 TGATGATCAA AGTGAAGAAC CACCAGACGC CGCCGACGAT GCGTCCGGAC AGGGACCTGG
     1621 GGGAAATGTC ACATCCTTGC cGCATGAAGG CCCCCAGGGA GAACCACAGG CTGTTGAATA
     1681 TGCCAAACTC ATTTGACTGG TCACTGGTTG TCTGGTCTCG TCCCTCTTCA AACTCTTCGC
     1741 TGTGCCATTC GTAGGGGCTG AAACGGCTGA CCAGGAAGAG GACGACGCTC ACTCCAATGT
     1801 AGGCAAACAC TATACACATC CAGATCTCAT AGGCCAAAGG GTCAAGAAAG GAGAAGACAC
     1861 CTGGCTTGGA CTTCTGTGGC TTCTTAATCA TAATGGAGAT TCCCAAACTC ATGAATGGCT
     1921 TGGAGAAGTC GATGACTTCC TCCCGGACCA AGGTTATGGT CAAGGGAGCC ACAGCCACGT
     1981 CTGCTCTTCC ATAGACCAGT TCTCCCACCA TGCCATTCCA AGCCTTTGTG TCGGGATCCC
     2041 GGGCTCCATA TTTGCCGTCG CTGACAATCT CAAGTCGGTA GGAGTAGCCC ACGTGCTTGG
     2101 CGATCTCTGC AGCCAGCTCC ACACAGTAGC CCTCATAGCG GTCATTGCCC TCAAACTGGT
     2161 TGGCATTCTT TTTAAGCATC ACGTAAGGAT CTTCGAGGAT AGTAGTGACG ATGTAGGTCC
     2221 TATTCTGGAC GCTTGAGTTG TCCCCTCCAG CCTGAGCGTC GGTGGCTGCG GGGACAAATT
     2281 TATCGTCTTC ATTCCAGTAA CCAATCTTTC GGATTCCATC ATGTTTCATT TCGATCACGT
     2341 GGAGGGTGTA ATTGGTCCGG CGCCCTTTCT CGTTGAACTG CACATTTCCT GTCAAACCTT
     2401 CGAAGCGCAC CTGCTGCAGG GCTCTCTGGA TGTCGATCCC TTGACCCCAG GGCACAGCTG
     2461 GGTTAGCCAG ACAGTCCCCA GCATTCCCCC GGCGGGATAT GTCAATCCTC TGCCTCCGCA
     2521 GGCTTTGGAA GGCCTCAGCC ATCACCTTGA CACCATCATA GGTGAGAGCA GAAGTGTACT
     2581 TTGGCCTCTT CCAGTCCACC CTGGTATGGT CTCGGGAGTC ACTTGTCCTC CATTGCTGCA
     2641 TGATTCTGGC TGGGATCGTG TCTGTGTAGT TCACCAGCTG GAAACCTGTC ACATTGGCTC
     2701 CGCTCTCCTT GAACTTATTT AAGTCAATGT CCATGAAGCC CAGATTGGCG AGGATGTAGT
     2761 GGTACCCGAT GCCATTCTTT TCTAGCTTCA CGATCTGGCC CAGGATGGCG TTGAGGCGTT
     2821 CTGATTCACA GTCAACCACC ACCAGCCTCT CCTTTTTCTT CTCCAGGTCC TGAAAGAGCA
     2881 TCCGGTATCC TTCCTCGGTG GTTGTCAGAA TGTTGACAGC CGTTACCTGC CAGTTCTTCT
     2941 CTGCGGCTGT ATCCAAGACT CTCTGCAGGA CTGACAGGCC CCGGTCAGCA TCATAAATGT
     3001 AGACAAAGGT TTGCCACTTG TAATGGTCGA TAATGCTAAT GAGAGCTTCC TGTAGTTCCG
     3061 GGCGTAGCTG AAGGACAAAT TGATTGGATG TGTCAACAGG AAAACTTGGA GTAATGAAGC
     3121 ACACATGGAG GGCCCCACAG AAGGAGGTCA GCATGTTGAC AGTCCTTCGT TCATAAAATC
     3181 CAAAGATGGC ATAGACTCCT TTGGAGAACT GGGAACAGAA ACGGTAAGTC ATCTCAAAGC
     3241 TGTCGCTGAT GTTCACAATA TCGATCTGGG GAAGCAGCTT GGGGGGCTCC GTGAGTTGTG
     3301 ACAAAGCAAA CCTAAAAGCC GCATGTTCCT GTGATTGTTG GTTTGGAAAT AACCCCCCTA
     3361 TCTGGATATT GTTGGGGAAa ttACCGGTAG ATCCACCTTT GTATAGTTCA TCCATGCCAT
     3421 GTGTAATCCC AGCAGCTGTT ACAAACTCAA GAAGGACCAT GTGGTCTCTC TTTTCGTTGG
     3481 GATCTTTCGA AAGAGTAGAA GTTGTAAACA GGTAATGGTT GTCTGGTAAA AGGACGGGCC
     3541 CATCGCCAAT TGGAGTATTT TGTTGATAAT GGTCTGCTAG TTGAACGCCT CCATCTTCAA
     3601 TGTTGTGTCT AATTTTGAAG TTAGCTTTGA TTCCATTCTT TTGTTTGTCT GCCATGATGT
     3661 ACACCTGGTG ATCGTTATAG TTGTATTCCA ATTTGTGTCC AAGAATGTTT CCATCTTCTT
     3721 TAAAATCAAT ACCTTTTAAC TCGATTCTAT TAACAAGGGT ATCACCTTCA AACTTGACTT
     3781 CAGCACGTGT CTTGTAGTTC CCGTCATCTT TGAAAAATAT AGTTCTTTCC TGTACATAAC
     3841 CTTCGGGCAT GGCACTCTTG AAAAAGTCAT GCCGTTTCAT ATGATCTGGG TATCTTGAAA
     3901 AGCATTGAAC ACCATAAGTT AAAGTAGTGA CAAGTGTTGG CCAAGGAACA GGTAGTTTTC
     3961 CAGTAGTGCA AATAAATTTA AGGGTAAGTT TTCCGTATGT TGCATCACCT TCACCCTCTC
     4021 CACTGACAGA AAATTTGTGC CCATTAACAT CACCATCTAA TTCAACAAGA ATTGGGACAA
     4081 CTCCAGTGAA AAGTTCTTCT CCTTTACTGT TAGAAGAGAC ACCAAAAATC AGTCCCCATA
     4141 AAACAGGAGA AAGGAGGACA GAAATATGCA TAATCTTTTG CATTTCCAAG AAATAACTTC
     4201 GTATAAAGTA TCCTATACGA AGTTATTTGC CTTAACCCAG AAATTATCAC TGTTATATAA
     4261 CTTCGTATAA TGTATGCTAT ACGAAGTTAT GATAATCAAC CTCTGGATTA CAAAATTTGT
     4321 GAAAGATTGA CTGGTATTCT TAACTATGTT GCTCCTTTTA CGCTATGTGG ATACGCTGCT
     4381 TTAATGCCTT TGTATCATGC TATTGCTTCC CGTATGGCTT TCATTTTCTC CTCCTTGTAT
     4441 AAATCCTGGT TAGTTCTTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG
     4501 ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TTATTTGTGA AATTTGTGAT
     4561 GCTATTGCTT TATTTGTAAC CATCTAGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
     4621 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
     4681 GTTTCAGGTT CAGGGGGAGA TGTGGGAGGT TTTTTAAAGC GGCCGCAGGA ACCCCTAGTG
     4741 ATGGAGTTGG CCACTCCCTC TCTGCGCGCT CGCTCGCTCA CTGAGGCCGG GCGACCAAAG
     4801 GTCGCCCGAC GCCCGGGCTT TGCCCGGGCG GCCTCAGTGA GCGAGCGAGC GCGCAGCTGC
     4861 CTGCAGGGGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC
     4921 ATACGTCAAA GCAACCATAG TACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
     4981 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT
     5041 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC
     5101 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTTGG
     5161 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG
     5221 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
     5281 CGGGCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG
     5341 AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTTAT
     5401 GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGCCC CGACACCCGC
     5461 CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG
     5521 CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG
     5581 CGAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG
     5641 TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT
     5701 TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC
     5761 AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT
     5821 TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG
     5881 ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA
     5941 AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC
     6001 TGCTATGTGG CGCGGTATTA TCCCGTATTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA
     6061 TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG
     6121 ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG
     6181 CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA
     6241 TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA
     6301 ACGACGAGCG TGACACCACG ATGCCTGTAG CAATGGCAAC AACGTTGCGC AAACTATTAA
     6361 CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA
     6421 AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT
     6481 CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC
     6541 CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA
     6601 GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT
     6661 ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA
     6721 AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG
     6781 CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA
     6841 TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG
     6901 AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG
     6961 TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT
     7021 ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA
     7081 CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG
     7141 GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC
     7201 GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA
     7261 GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC
     7321 TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT
     7381 CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT
     7441 TTTGCTGGCC TTTTGCTCAC ATGT
//