LOCUS       pAAV-hSyn1-DIO-eGFP_V3  4792 bp ds-DNA     circular     02-OCT-2024
DEFINITION  .
FEATURES             Location/Qualifiers
     LTR             1..141
                     /label="ITR 5'"
     ITR element     12..141
                     /label="Flop"
     ITR element     complement(38..46)
                     /label="ITR-C"
     Should avoid    complement(46..50)
                     /label="rep-binding seq"
     ITR element     50..58
                     /label="ITR-C"
     ITR element     complement(60..68)
                     /label="ITR-B"
     Should avoid    68..72
                     /label="rep-binding seq"
     ITR element     72..80
                     /label="ITR-B"
     ITR element     81..121
                     /label="ITR-A"
     misc_feature    complement(107..112)
                     /label="FUS5"
     Should avoid    complement(116..121)
                     /label="trs"
     Should avoid    118..123
                     /label="trs (AAV2)"
     ITR element     complement(121..141)
                     /label="ITR-D"
     misc_feature    149..617
                     /label="hSyn1 promoter"
     misc_feature    complement(152..157)
                     /label="FUS1"
     misc_feature    complement(153..158)
                     /label="FUS5"
     misc_feature    complement(289..294)
                     /label="FUS5"
     misc_feature    complement(299..304)
                     /label="FUS2"
     misc_feature    444..449
                     /label="FUSmouse1"
     misc_feature    618..651
                     /label="Lox2272"
     misc_feature    682..715
                     /label="LoxP"
     CDS             complement(789..1505)
                     /label="EGFP"
     CDS             complement(789..1505)
                     /label="Translation 1505-789"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     Should avoid    875..879
                     /label="rep-binding seq"
     regulatory      complement(1502..1511)
                     /label="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)"
     misc_feature    complement(1521..1554)
                     /label="Lox2272"
     misc_feature    complement(1521..1554)
                     /label="Lox2272 (inv)"
     misc_feature    complement(1585..1618)
                     /label="LoxP (inv)"
     misc_recomb     complement(1585..1618)
                     /label="LoxP"
     misc_feature    1620..2046
                     /label="CW3SL"
     misc_feature    1627..1632
                     /label="FUS4"
     misc_feature    1628..1633
                     /label="FUS1"
     Should avoid    1716..1720
                     /label="rep-binding seq"
     misc_feature    1734..1739
                     /label="FUSmouse1"
     regulatory      1867..2046
                     /label="SL polyA signal"
     misc_feature    complement(1868..1873)
                     /label="polyA motif"
     misc_feature    complement(1897..1902)
                     /label="polyA motif"
     misc_feature    complement(1917..1922)
                     /label="polyA motif"
     misc_feature    complement(1946..1951)
                     /label="polyA motif"
     misc_feature    1971..1976
                     /label="polyA motif"
     ITR element     2055..2075
                     /label="ITR-D"
     ITR element     complement(2055..2184)
                     /label="Flop"
     LTR             2056..2196
                     /label="ITR 3'"
     Should avoid    complement(2073..2078)
                     /label="trs (AAV2)"
     Should avoid    2075..2080
                     /label="trs"
     ITR element     complement(2075..2115)
                     /label="ITR-A"
     misc_feature    2084..2089
                     /label="FUS5"
     ITR element     complement(2116..2124)
                     /label="ITR-B"
     Should avoid    complement(2124..2128)
                     /label="rep-binding seq"
     ITR element     2128..2136
                     /label="ITR-B"
     ITR element     complement(2138..2146)
                     /label="ITR-C"
     Should avoid    2146..2150
                     /label="rep-binding seq"
     ITR element     2150..2158
                     /label="ITR-C"
     ITR element     2159..2199
                     /label="ITR-A variant 1"
     Should avoid    complement(2255..2259)
                     /label="rep-binding seq"
     rep_origin      2270..2725
                     /label="M13 origin"
     rep_origin      2287..2593
                     /label="F1 ori"
     misc_feature    2370..2375
                     /label="FUSmouse1"
     misc_feature    2371..2376
                     /label="FUS3"
     misc_feature    2401..2406
                     /label="FUS2"
     Should avoid    2537..2541
                     /label="rep-binding seq"
     misc_feature    complement(2878..2883)
                     /label="FUS1"
     misc_feature    complement(2879..2884)
                     /label="FUS4"
     misc_feature    complement(2997..3002)
                     /label="FUS2"
     misc_feature    complement(3024..3029)
                     /label="polyA motif"
     CDS             3112..3972
                     /label="bla"
     CDS             complement(3112..3972)
                     /label="Ampicillin"
     misc_feature    3143..3148
                     /label="FUS3"
     Should avoid    complement(3221..3226)
                     /label="trs (AAV2)"
     CDS             3310..3969
                     /label="AmpR"
     Should avoid    complement(3507..3512)
                     /label="trs"
     Should avoid    3509..3514
                     /label="trs (AAV2)"
     misc_feature    3710..3715
                     /label="FUSmouse1"
     misc_feature    complement(3793..3798)
                     /label="polyA motif"
     rep_origin      4067..4749
                     /label="ColE1 origin"
     rep_origin      4117..4790
                     /label="pUC origin"
     Should avoid    complement(4131..4135)
                     /label="rep-binding seq"
     Should avoid    4234..4239
                     /label="trs (AAV2)"
     misc_feature    4552..4557
                     /label="FUSmouse1"
     misc_feature    complement(4632..4637)
                     /label="FUS2"
     misc_feature    4673..4678
                     /label="FUS4"
     misc_feature    4674..4679
                     /label="FUS1"
     ITR element     complement(4789..37)
                     /label="ITR-A variant 2"
ORIGIN
        1 CCTGCAGGCA GCTGCGCGCT CGCTCGCTCA CTGAGGCCGC CCGGGCAAAG CCCGGGCGTC
       61 GGGCGACCTT TGGTCGCCCG GCCTCAGTGA GCGAGCGAGC GCGCAGAGAG GGAGTGGCCA
      121 ACTCCATCAC TAGGGGTTCC TGCGGCCGCT GCAGAGGGCC CTGCGTATGA GTGCAAGTGG
      181 GTTTTAGGAC CAGGATGAGG CGGGGTGGGG GTGCCTACCT GACGACCGAC CCCGACCCAC
      241 TGGACAAGCA CCCAACCCCC ATTCCCCAAA TTGCGCATCC CCTATCAGAG AGGGGGAGGG
      301 GAAACAGGAT GCGGCGAGGC GCGTGCGCAC TGCCAGCTTC AGCACCGCGG ACAGTGCCTT
      361 CGCCCCCGCC TGGCGGCGCG CGCCACCGCC GCCTCAGCAC TGAAGGCGCG CTGACGTCAC
      421 TCGCCGGTCC CCCGCAAACT CCCCTTCCCG GCCACCTTGG TCGCGTCCGC GCCGCCGCCG
      481 GCCCAGCCGG ACCGCACCAC GCGAGGCGCG AGATAGGGGG GCACGGGCGC GACCATCTGC
      541 GCTGCGGCGC CGGCGACTCA GCGCTGCCTC AGTCTGCGGT GGGCAGCGGA GGAGTCGTGT
      601 CGTGCCTGAG AGCGCAGATA ACTTCGTATA GGATACTTTA TACGAAGTTA TGCAGAATGG
      661 TAGCTGGATT GTAGCTGCTA TATAACTTCG TATAGCATAC ATTATACGAA GTTATCTAAA
      721 TTTTAACACT CTCGATGCCA TATACGTTGT AACCTTCCTT ATAAGTTGCA AAATTCTGGG
      781 AATTCCTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC CGGCGGCGGT CACGAACTCC
      841 AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC TCAGGGCGGA CTGGGTGCTC
      901 AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA TGGGGGTGTT CTGCTGGTAG
      961 TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC GGATCTTGAA GTTCACCTTG
     1021 ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT GGCTGTTGTA GTTGTACTCC
     1081 AGCTTGTGCC CCAGGATGTT GCCGTCCTCC TTGAAGTCGA TGCCCTTCAG CTCGATGCGG
     1141 TTCACCAGGG TGTCGCCCTC GAACTTCACC TCGGCGCGGG TCTTGTAGTT GCCGTCGTCC
     1201 TTGAAGAAGA TGGTGCGCTC CTGGACGTAG CCTTCGGGCA TGGCGGACTT GAAGAAGTCG
     1261 TGCTGCTTCA TGTGGTCGGG GTAGCGGCTG AAGCACTGCA CGCCGTAGGT CAGGGTGGTC
     1321 ACGAGGGTGG GCCAGGGCAC GGGCAGCTTG CCGGTGGTGC AGATGAACTT CAGGGTCAGC
     1381 TTGCCGTAGG TGGCATCGCC CTCGCCCTCG CCGGACACGC TGAACTTGTG GCCGTTTACG
     1441 TCGCCGTCCA GCTCGACCAG GATGGGCACC ACCCCGGTGA ACAGCTCCTC GCCCTTGCTC
     1501 ACCATGGTGG CGACCTCGAG ATAACTTCGT ATAAAGTATC CTATACGAAG TTATTTGCCT
     1561 TAACCCAGAA ATTATCACTG TTATATAACT TCGTATAATG TATGCTATAC GAAGTTATGA
     1621 TAATCAACCT CTGGATTACA AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC
     1681 TCCTTTTACG CTATGTGGAT ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG
     1741 TATGGCTTTC ATTTTCTCCT CCTTGTATAA ATCCTGGTTA GTTCTTGCCA CGGCGGAACT
     1801 CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG CTGTTGGGCA CTGACAATTC
     1861 CGTGGTGTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA TTTGTAACCA TCTAGCTTTA
     1921 TTTGTGAAAT TTGTGATGCT ATTGCTTTAT TTGTAACCAT TATAAGCTGC AATAAACAAG
     1981 TTAACAACAA CAATTGCATT CATTTTATGT TTCAGGTTCA GGGGGAGATG TGGGAGGTTT
     2041 TTTAAAGCGG CCGCAGGAAC CCCTAGTGAT GGAGTTGGCC ACTCCCTCTC TGCGCGCTCG
     2101 CTCGCTCACT GAGGCCGGGC GACCAAAGGT CGCCCGACGC CCGGGCTTTG CCCGGGCGGC
     2161 CTCAGTGAGC GAGCGAGCGC GCAGCTGCCT GCAGGGGCGC CTGATGCGGT ATTTTCTCCT
     2221 TACGCATCTG TGCGGTATTT CACACCGCAT ACGTCAAAGC AACCATAGTA CGCGCCCTGT
     2281 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC
     2341 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC
     2401 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG
     2461 CACCTCGACC CCAAAAAACT TGATTTGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA
     2521 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC
     2581 CAAACTGGAA CAACACTCAA CCCTATCTCG GGCTATTCTT TTGATTTATA AGGGATTTTG
     2641 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT
     2701 AACAAAATAT TAACGTTTAC AATTTTATGG TGCACTCTCA GTACAATCTG CTCTGATGCC
     2761 GCATAGTTAA GCCAGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT
     2821 CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG
     2881 AGGTTTTCAC CGTCATCACC GAAACGCGCG AGACGAAAGG GCCTCGTGAT ACGCCTATTT
     2941 TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA
     3001 AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC
     3061 ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT
     3121 CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT
     3181 CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT
     3241 TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT
     3301 TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTATTGAC
     3361 GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC
     3421 TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT
     3481 GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG
     3541 AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG
     3601 GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGTAGCA
     3661 ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA
     3721 CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT
     3781 CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC
     3841 ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG
     3901 AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT
     3961 AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT
     4021 CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC
     4081 CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT
     4141 TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA
     4201 CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC
     4261 TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC
     4321 TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT
     4381 GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT
     4441 AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG
     4501 ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA
     4561 GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG
     4621 GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA
     4681 CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC
     4741 AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GT
//