LOCUS       dna                     6215 bp    DNA              UNK 30-SEP-2024
DEFINITION  .
ACCESSION   dna
VERSION     dna
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    415..1656
                     /label="aclB upstream"
                     /description="Upstream region of the aclB gene"
     promoter        1674..2922
                     /label="Ptef1"
                     /description="tef1 promoter"
     misc_feature    2923..3943
                     /label="aclB 5'"
                     /description="5' part of the aclB gene"
     rep_origin      complement(4396..4984)
                     /label="ori"
                     /description="RIGHT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5155..6015)
                     /label="AmpR"
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6016..6120)
                     /label="AmpR promoter"
                     /gene="bla"
     source          1..6215
                     /label="dna"
                     /description="Record of building history"
                     /building_history_2="QUEEN.dna_dict['dna']–=–QUEEN(seq='TCG
                     CGCGTTTCGGTGATGACGGTGAAAACCTCTGACACATGCAGCTCCCGGAGACGGTCACA
                     GCTTGTCTGTAAGCGGATGCCGGGAGCAGACAAGCCCGTCAGGGCGCGTCAGCGGGTGT
                     TGGCGGGTGTCGGGGCTGGCTTAACTATGCGGCATCAGAGCAGATTGTACTGAGAGTGC
                     ACCATATGCGGTGTGAAATACCGCACAGATGCGTAAGGAGAAAATACCGCATCAGGCGC
                     CATTCGCCATTCAGGCTGCGCAACTGTTGGGAAGGGCGATCGGTGCGGGCCTCTTCGCT
                     ATTACGCCAGCTGGCGAAAGGGGGATGTGCTGCAAGGCGATTAAGTTGGGTAACGCCAG
                     GGTTTTCCCAGTCACGACGTTGTAAAACGACGGCCAGTGAATTCGAGCTCGGTACCCAG
                     TCGGTGAGCATCGTCATGTGCGCCCGTTAGCAGCAGGCAGGAATCGAAATATTTTCTTA
                     GGCGGCATCTTTACGTAGTAGGATGCAGGCGATGGATGAGGTGTACTCCGGTTTACTGT
                     GTACTTTGTATACTACTAGTGGGAAGGTACATTGGCATCCCTGGGCCAACAAGAAAAAA
                     AAAATATCAGACCGTTCATCGACCCCACCTTTAGTCCCGACCAGATCATCTTACAGAAA
                     TAGTGCCTTGGGCCTTAACTTTCCGGAGTCGAGACTTGCTTGAAGATGGCCCGATTATC
                     CTGCTCATTACCCTTTTTGACCGTAGTCAGTATTGTATATACGGTGTACTTCAGATCAC
                     TCTTGGGGTCAGTATTATGAATACCGTGTACTTCGGGTCACCACTGGAGTCAGTCCAGT
                     TTATACGGTGTACTTCAGATCACCGCCCAGGCAGGAAAAAAACAAGACTTGAGTTTGTT
                     GAGCAACATTATCAACCCCCCCAAAAATAATACACTGCAGATTAGCTTGTCGCATACTC
                     CGCACTCCATCAGCAGTACTGTGTACGCCGTCACACCAGCCCTAGCTAGCTCTTTCATT
                     CCACCACCGTAGCTTTTCCTCTAAATGGTTCCTGTGGCTTTCCCGAGTCCCGGTTAGCT
                     AGACTAGATTTTCCAATAAGAAATTCTCATCGGGAGGTTATTATTAGGACCTTTTCAAG
                     TCCCGAAATCCGAACATAGCCTCAGGCCAGACTACTATTTTCCCAACTGGTCCCGCATT
                     GCGCCCCCCGCCGTTTTCTCAAGACCGTATAGTACCGGCCCATACCGCCCACCCATACG
                     ATTACCTGTCTGATACATACCAACCATACGCCATACATACTTCAGACTCCGGGCTTCTG
                     TACTTCACCTCAAAAATTATTATTAGCAGCTCCATAATTTCTTTACTCCGTACCAACCC
                     CCAATCTTAACCCGATATCGTTCCCCTCTGTTGTTATTTTTTTTTCTTCTCCCATTCCG
                     TCTTAACTCATCGATTAATCCACTTCATTCCCCCTCCAATTCCAATTTCTCCATCCTCC
                     TTCCTTGGTTATCGTCCTTATCCTCCTCTCCCATCTATCTTCTCCTTCCCTCCCAGGTC
                     GCTATTACTTGTTGCGACTTACTCCTCTATATCATTTAACTCACCTGCATACCCACCTA
                     ATAATACCTTCTTGTTTCCTTTGATTGTTTTACGTTTGGAGCTCTGTTGACAGGTATGC
                     TCTGCAGGCATGCAAGCTGTAATCGCCCGTTTGGGTTTGGGGGAACACCTTCATCCATA
                     GTAAGCTTGTGCGTTGTTCCCAAACACGGGACCTGCTAGCCATAAGGAATTTTTAGATA
                     GGGATTATTATGATTTTTGTTCTACGTATTCTATTAGATTTTTTGTTTAAATTTTGTTT
                     TTTTGTTTTTTTGTTTTTTTTGTTCCTTGTCTATATGAAAAAAATTTTCATCTACATAC
                     TACGTAGATTGTCACCGCTCAGGAGGGGCAACAACAGATCTTACCTAAGTACACTAGTA
                     AGGTCCGAGACAGTAAGGGATTGATCTAAATGAGGGGCACACAGCAGCGATCATATGTG
                     AAAGAAAAACTAGAAAGGCAAAAAAAGAAAAGAAAAAGAGAAGAAAATTTTTCCTAGGT
                     ATCCGTAGTCTGTCAACATTGCCTCTTTGCCTCCTAACAGCATATGTACGGAGTATCTG
                     TACGTACTCTAGATAGCGAGAGTAAAAAAAAAAAAGATTTTCACTGTGGACCAGACAGG
                     CGCCACTCGGCCGGGCCACAGCTGCTTGGGTGTTGACCGGGAGCGGACCAATTAAGGAC
                     TCGAACGACCGCGGGGTTCAAATGCAAACAAGTACAACACGCAGCAAACGAAGCAGCCC
                     ACCACTGCGTTGATGCCCAGTTTGACTGTCCGAAATCCACCGGAAAGGTGGAAACATAC
                     TATGTAACAATCAGAGGGAAGAAAAAATTTTTATCGACGAGGCAGGATAGTGACTGATG
                     GTGGGGTCATGGTCGGGTCTCCGAGCGAAAGAGAACCAAGGAAACAAGATCAACGAGGT
                     TGGTGTACCCAAAAGGCCGCAGCAACAAGAGTCATCGCCCAAAAGTCAACAGTCTGGAA
                     GAGACTCCGCCGTGCAGATTCTGCGTCGGTCCCGCACATGCGTGGTGGGGGCATTACCC
                     CTCCATGTCCAATGATAAGGGCGGCGGTCGAGGGCTTAAGCCCGCCCACTAATTCGCCT
                     TCTCGCTTGCCCCTCCATATAAGGATTCCCCCTCCTTCCCCTCCCACAACTTTTTTCCT
                     TCTTTCTCTCTTCGTCCGCATCAGTACGTATATCTTTCCCCCATACCTCCTTTCCTACT
                     CTTCTTCCATTCATTCAACTCTTCTCCTTACTGACATCTGTTTTGCTCAGTACCTCTAC
                     GCGATCAGCCGTAGTATCTGAGCAAGCTTCTCTACAGAATCTTTCTAGTATCTTACAAA
                     GAACTACAAAGTTCGCACCACCTTCAAAATGTCTTCTACCGCTCCTCTTGTCAACACCG
                     CCAATGGCGGCTCTGCCAATGACAACATCACCCGCTTCGCACCCCCCAGCCGTGTGCTC
                     TCTCCTCTCAACCATGCCCTCTTCCACAACAAGACCAGATGTTTCGTATAGTGAGTACT
                     TCCTGTCTTCTGGAGGTCAACGTCATATATTGACCGTTGTAATAGTGGTATGCAGCCCA
                     AGGCCGTGCAGGGCATGCTCGACTTCGATTTCATCTGCAAGCGCAGCACACCCTCAGTC
                     GCCGGTATCATCTACACTTTCGGTGGTCAATTCGTGAGCAAGATGTACTGGGGTACTAG
                     CGAGACTTTGCTCCCTGTCTACCAGGACGTTTCCAAGGCCATGGCCAAGCACCCCGATG
                     TTGACACTGTTGTCAACTTCGCCTCCTCCCGTTCCGTGTACAGCTCTACCATGGAGTTG
                     ATGAACTGCCCTCAGATTAAGTCCATTGCCATCATTGCCGAGGGTGTCCCAGAAAGAGT
                     AGGTCCTTGATCTCTGCCAAGCTAGACTTCGACTGACAGTGGTAATAGCGCGCTCGTGA
                     GATTCTCGTCACTGCTAAGGAGAAGGGCATCACCATTATTGGACCCGCTACCGTTGGAG
                     GTATCAAGCCTGGTGCTTTCAAGATTGGTAACACTGGTGGTATGATGGACAACATTGTG
                     GCTTCCAAGCTCTACCGTAAGGGCTCTGTCGGCTACGTCTCCAAGTCCGGTGGTATGTC
                     CAATGAATTGAACAACATTGTCTCTCAGAATACCGATGGTGTCTACGAGGGTGTTGCCA
                     TTGGTGGTGATCGTTACCCTGGTACCACTTTCATCGACCATTTGCTGCGTTACCAGAAC
                     GAGCCGGAGTGCAAGATCCTTGTGTTGCTCGGCGAAGTCGGTGGTGTTGAGGAGTACCG
                     TGTCATCGAGGCCGTCAAGAACGGCACCATCACCAAGCCCATTGTTGCCTGGGCTATTG
                     GTACTTGCGCCAGCATGTTCAAGACGGAGGTCCAGTTCGGTCACGCGGGGATCCTCTAG
                     AGTCGACCTGCAGGCATGCAAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGA
                     AATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGC
                     CTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTT
                     TCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGA
                     GGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGT
                     CGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
                     AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAAC
                     CGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCA
                     CAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGG
                     CGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGA
                     TACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAG
                     GTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
                     TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGA
                     CACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGT
                     AGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAG
                     TATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCT
                     TGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGAT
                     TACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
                     CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATC
                     TTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGA
                     GTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCT
                     GTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGG
                     GAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGC
                     TCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTG
                     CAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGT
                     TCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACG
                     CTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACAT
                     GATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGA
                     AGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTAC
                     TGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCT
                     GAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACC
                     GCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAA
                     ACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCA
                     ACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGG
                     CAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTT
                     CCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATAT
                     TTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG
                     CCACCTGACGTCTAAGAAACCATTATTATCATGACATTAACCTATAAAAATAGGCGTAT
                     CACGAGGCCCTTTCGTC',–topology='circular')"
                     /building_history_2=""
                     /building_history_2="dna-LTJ49ZKDAOEDBUNSU2OMTKGW"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccagtcgg
      421 tgagcatcgt catgtgcgcc cgttagcagc aggcaggaat cgaaatattt tcttaggcgg
      481 catctttacg tagtaggatg caggcgatgg atgaggtgta ctccggttta ctgtgtactt
      541 tgtatactac tagtgggaag gtacattggc atccctgggc caacaagaaa aaaaaaatat
      601 cagaccgttc atcgacccca cctttagtcc cgaccagatc atcttacaga aatagtgcct
      661 tgggccttaa ctttccggag tcgagacttg cttgaagatg gcccgattat cctgctcatt
      721 accctttttg accgtagtca gtattgtata tacggtgtac ttcagatcac tcttggggtc
      781 agtattatga ataccgtgta cttcgggtca ccactggagt cagtccagtt tatacggtgt
      841 acttcagatc accgcccagg caggaaaaaa acaagacttg agtttgttga gcaacattat
      901 caaccccccc aaaaataata cactgcagat tagcttgtcg catactccgc actccatcag
      961 cagtactgtg tacgccgtca caccagccct agctagctct ttcattccac caccgtagct
     1021 tttcctctaa atggttcctg tggctttccc gagtcccggt tagctagact agattttcca
     1081 ataagaaatt ctcatcggga ggttattatt aggacctttt caagtcccga aatccgaaca
     1141 tagcctcagg ccagactact attttcccaa ctggtcccgc attgcgcccc ccgccgtttt
     1201 ctcaagaccg tatagtaccg gcccataccg cccacccata cgattacctg tctgatacat
     1261 accaaccata cgccatacat acttcagact ccgggcttct gtacttcacc tcaaaaatta
     1321 ttattagcag ctccataatt tctttactcc gtaccaaccc ccaatcttaa cccgatatcg
     1381 ttcccctctg ttgttatttt tttttcttct cccattccgt cttaactcat cgattaatcc
     1441 acttcattcc ccctccaatt ccaatttctc catcctcctt ccttggttat cgtccttatc
     1501 ctcctctccc atctatcttc tccttccctc ccaggtcgct attacttgtt gcgacttact
     1561 cctctatatc atttaactca cctgcatacc cacctaataa taccttcttg tttcctttga
     1621 ttgttttacg tttggagctc tgttgacagg tatgctctgc aggcatgcaa gctgtaatcg
     1681 cccgtttggg tttgggggaa caccttcatc catagtaagc ttgtgcgttg ttcccaaaca
     1741 cgggacctgc tagccataag gaatttttag atagggatta ttatgatttt tgttctacgt
     1801 attctattag attttttgtt taaattttgt ttttttgttt ttttgttttt tttgttcctt
     1861 gtctatatga aaaaaatttt catctacata ctacgtagat tgtcaccgct caggaggggc
     1921 aacaacagat cttacctaag tacactagta aggtccgaga cagtaaggga ttgatctaaa
     1981 tgaggggcac acagcagcga tcatatgtga aagaaaaact agaaaggcaa aaaaagaaaa
     2041 gaaaaagaga agaaaatttt tcctaggtat ccgtagtctg tcaacattgc ctctttgcct
     2101 cctaacagca tatgtacgga gtatctgtac gtactctaga tagcgagagt aaaaaaaaaa
     2161 aagattttca ctgtggacca gacaggcgcc actcggccgg gccacagctg cttgggtgtt
     2221 gaccgggagc ggaccaatta aggactcgaa cgaccgcggg gttcaaatgc aaacaagtac
     2281 aacacgcagc aaacgaagca gcccaccact gcgttgatgc ccagtttgac tgtccgaaat
     2341 ccaccggaaa ggtggaaaca tactatgtaa caatcagagg gaagaaaaaa tttttatcga
     2401 cgaggcagga tagtgactga tggtggggtc atggtcgggt ctccgagcga aagagaacca
     2461 aggaaacaag atcaacgagg ttggtgtacc caaaaggccg cagcaacaag agtcatcgcc
     2521 caaaagtcaa cagtctggaa gagactccgc cgtgcagatt ctgcgtcggt cccgcacatg
     2581 cgtggtgggg gcattacccc tccatgtcca atgataaggg cggcggtcga gggcttaagc
     2641 ccgcccacta attcgccttc tcgcttgccc ctccatataa ggattccccc tccttcccct
     2701 cccacaactt ttttccttct ttctctcttc gtccgcatca gtacgtatat ctttccccca
     2761 tacctccttt cctactcttc ttccattcat tcaactcttc tccttactga catctgtttt
     2821 gctcagtacc tctacgcgat cagccgtagt atctgagcaa gcttctctac agaatctttc
     2881 tagtatctta caaagaacta caaagttcgc accaccttca aaatgtcttc taccgctcct
     2941 cttgtcaaca ccgccaatgg cggctctgcc aatgacaaca tcacccgctt cgcacccccc
     3001 agccgtgtgc tctctcctct caaccatgcc ctcttccaca acaagaccag atgtttcgta
     3061 tagtgagtac ttcctgtctt ctggaggtca acgtcatata ttgaccgttg taatagtggt
     3121 atgcagccca aggccgtgca gggcatgctc gacttcgatt tcatctgcaa gcgcagcaca
     3181 ccctcagtcg ccggtatcat ctacactttc ggtggtcaat tcgtgagcaa gatgtactgg
     3241 ggtactagcg agactttgct ccctgtctac caggacgttt ccaaggccat ggccaagcac
     3301 cccgatgttg acactgttgt caacttcgcc tcctcccgtt ccgtgtacag ctctaccatg
     3361 gagttgatga actgccctca gattaagtcc attgccatca ttgccgaggg tgtcccagaa
     3421 agagtaggtc cttgatctct gccaagctag acttcgactg acagtggtaa tagcgcgctc
     3481 gtgagattct cgtcactgct aaggagaagg gcatcaccat tattggaccc gctaccgttg
     3541 gaggtatcaa gcctggtgct ttcaagattg gtaacactgg tggtatgatg gacaacattg
     3601 tggcttccaa gctctaccgt aagggctctg tcggctacgt ctccaagtcc ggtggtatgt
     3661 ccaatgaatt gaacaacatt gtctctcaga ataccgatgg tgtctacgag ggtgttgcca
     3721 ttggtggtga tcgttaccct ggtaccactt tcatcgacca tttgctgcgt taccagaacg
     3781 agccggagtg caagatcctt gtgttgctcg gcgaagtcgg tggtgttgag gagtaccgtg
     3841 tcatcgaggc cgtcaagaac ggcaccatca ccaagcccat tgttgcctgg gctattggta
     3901 cttgcgccag catgttcaag acggaggtcc agttcggtca cgcggggatc ctctagagtc
     3961 gacctgcagg catgcaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     4021 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     4081 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     4141 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     4201 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     4261 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     4321 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     4381 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     4441 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     4501 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     4561 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     4621 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     4681 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     4741 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     4801 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     4861 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     4921 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     4981 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     5041 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     5101 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     5161 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     5221 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     5281 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     5341 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     5401 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     5461 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     5521 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     5581 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     5641 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     5701 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     5761 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     5821 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     5881 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     5941 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     6001 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     6061 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     6121 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
     6181 ctataaaaat aggcgtatca cgaggccctt tcgtc
//
