LOCUS       pKR016_-_pLenti       13870 bp DNA     circular SYN 15-SEP-2023
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13870)
  TITLE     New vectors Aug. 2010
REFERENCE   2  (bases 1 to 13870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Sep 19, 2024 from SnapGene 7.2.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     froze clone M8Y #7 as pKR020
COMMENT     Sequence Label: pLenti CMV/TO GFP-MDC1 (779-2)
FEATURES             Location/Qualifiers
     source          1..13870
                     /lab_host="Escherichia coli"
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     41..58
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    175..196
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(211..216,217..234,235..241)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    249..265
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     254..276
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     273..289
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     273..289
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     308..328
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        310..328
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        356..582
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             583..763
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    810..935
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1428..1661
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1846..1890
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2157..2273
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2296..2599
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2600..2803
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2754..2774
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2805..2823
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2826..2844
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2885..2909
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2948..2957
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2954..3499
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="fast-reacting variant, optimized for mammalian 
                     expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHRIIFLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYSHLAALAGNPAATAAVKTALSGNPVPILIPCHRVVQGDLDVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
     CDS             3533..9802
                     /label=MDC1
     protein_bind    complement(10014..10038)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    10069..10657
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10122..10142)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10540..10551)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10744..10977
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    11049..11170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11086..11105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11140..11159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      11210..11345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11272..11291
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(11295..11306)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(11365..11384)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11366..11384)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11394..11411)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11394..11410)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11403..11425)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      11552..12007
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11639..11658)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11849..11870
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        12033..12137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12138..12206,12207..12998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12356..12375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13169..13757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13658..13677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
       61 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
      121 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
      181 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
      241 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
      301 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
      361 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
      421 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
      481 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
      541 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
      601 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
      661 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
      721 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
      781 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
      841 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
      901 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
      961 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     1021 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     1081 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     1141 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     1201 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     1261 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     1321 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     1381 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     1441 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     1501 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     1561 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     1621 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     1681 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     1741 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     1801 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     1861 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     1921 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     1981 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     2041 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
     2101 agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg gttaactttt
     2161 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca
     2221 acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat tttatcgata
     2281 agcttgggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     2341 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     2401 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     2461 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     2521 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     2581 agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg
     2641 gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg
     2701 gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat
     2761 gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta tcagtgatag
     2821 agatctccct atcagtgata gagatcgtcg actagtccag tgtggtggaa ttctgcagat
     2881 atcaacaagt ttgtacaaaa aagcaggctc caccatggga accaattcag tcgactagcg
     2941 ctaccgggcc accatggaca aagactgcga aatgaagcgc accaccctgg atagccctct
     3001 gggcaagctg gaactgtctg ggtgcgaaca gggcctgcac cgtatcatct tcctgggcaa
     3061 aggaacatct gccgccgacg ccgtggaagt gcctgcccca gccgccgtgc tgggcggacc
     3121 agagccactg atgcaggcca ccgcctggct caacgcctac tttcaccagc ctgaggccat
     3181 cgaggagttc cctgtgccag ccctgcacca cccagtgttc cagcaggaga gctttacccg
     3241 ccaggtgctg tggaaactgc tgaaagtggt gaagttcgga gaggtcatca gctacagcca
     3301 cctggccgcc ctggccggca atcccgccgc caccgccgcc gtgaaaaccg ccctgagcgg
     3361 aaatcccgtg cccattctga tcccctgcca ccgggtggtg cagggcgacc tggacgtggg
     3421 gggctacgag ggcgggctcg ccgtgaaaga gtggctgctg gcccacgagg gccacagact
     3481 gggcaagcct gggctgggta aattctgcag tcgacggtac cgcgggccca tcatggagga
     3541 cacccaggct attgactggg atgttgaaga agaggaggag acagagcaat ccagtgaatc
     3601 cttgaggtgt aacgtggagc cagtagggcg gctacatatc tttagtggtg cccatggacc
     3661 agaaaaagat ttcccactac acctcgggaa gaatgtggta ggccgaatgc ctgactgctc
     3721 tgtggccctg ccctttccat ctatctccaa acaacatgca gagattgaaa tcttagcctg
     3781 ggacaaggca cctatcctcc gagactgtgg gagccttaat ggtactcaaa tcctgagacc
     3841 tcctaaggtt ttgagccctg gggtgagtca ccgtctgagg gaccaggaat tgattctctt
     3901 tgctgacttg ctctgccagt accatcgcct ggatgtctct ctgccctttg tctcccgggg
     3961 ccctctgaca gtagaagaga cacccagagt acagggagaa actcaacccc agaggcttct
     4021 gttggctgag gactcggagg aggaagtaga ttttctttct gaaaggcgta tggtaaaaaa
     4081 atcaaggacc acatcttcct ctgtgatagt tccagagagt gatgaagagg ggcattcccc
     4141 ggtcctgggc ggccttgggc cgccttttgc cttcaatttg aacagtgaca cagatgtgga
     4201 agaaggtcag caaccagcca cagaggaggc ctcctcagct gccagaagag gtgccactgt
     4261 agaggcaaag cagtctgaag ctgaagttgt aactgaaatc cagcttgaaa aggatcagcc
     4321 tttagtgaag gagagggaca atgatacaaa agtcaagagg ggtgcaggga atggggtggt
     4381 tccagctggg gtgattctgg agaggagcca acctcctgga gaggacagtg acacagatgt
     4441 ggatgatgac agcaggcctc ctggaaggcc agctgaggtc catttggaaa gggctcagcc
     4501 ttttggcttc atcgacagcg acactgatgc ggaagaagag aggatcccag caaccccagt
     4561 tgtcattcct atgaagaaga ggaagatctt ccatggagta ggtacaaggg gtcctggagc
     4621 accaggcctg gcccatctgc aggagagcca ggctggtagt gatacagatg tggaagaagg
     4681 caaggcccca caggctgtcc ctctggagaa aagccaagct tccatggtta tcaacagcga
     4741 tacagatgac gaggaagaag tctcagcagc gctgactttg gcacatctga aagagagcca
     4801 gcctgctata tggaacagag atgcagaaga ggacatgccc caacgtgtgg tccttctgca
     4861 gcgaagccaa accaccactg agagagacag tgacacagac gtggaggagg aagagctccc
     4921 agtggaaaat agagaagctg tcctcaagga tcacacaaag attagagccc ttgttagagc
     4981 acattcagaa aaggaccaac ctccttttgg ggacagtgat gacagtgtgg aagcagataa
     5041 gagctcacct gggatccacc tggagagaag ccaagcctcc accacagtgg acatcaacac
     5101 acaagtggag aaggaagtcc cgccagggtc agccattatg catataaaga agcatcaggt
     5161 gtctgtggag gggacaaatc aaacagatgt gaaagcagtt gggggaccag caaagctgct
     5221 tgtggtatct ctagaggaag cctggcctct gcatggggac tgtgaaacag atgcagagga
     5281 gggcacctcc ctaacagcct cagtagttgc agatgtaaga aagagccagc ttccagcaga
     5341 aggggatgct ggggcagagt gggctgcagc tgttcttaag caggagagag ctcatgaggt
     5401 gggggcccag ggtgggccac ctgtggcaca agtggagcag gacctcccta tctcaagaga
     5461 gaacctcaca gatctggtgg tggacacaga cactctaggg gaatccaccc agccacagag
     5521 agagggagcc caggtcccca caggaaggga gagagaacaa catgtgggtg ggaccaagga
     5581 ctctgaagac aactatggtg attctgaaga tctggaccta caagctaccc agtgctttct
     5641 ggagaatcag ggcctggaag cagtccagag catggaggat gaacctaccc aggccttcat
     5701 gttgactcca ccccaagagc ttggcccttc ccattgcagc ttccagacaa caggtaccct
     5761 agatgaacca tgggaggtcc tggctacaca gccattctgt ctgagagagt ctgaggactc
     5821 tgagacccag ccttttgaca cgcaccttga ggcctatgga ccttgcctgt ctccacctag
     5881 ggcaatacca ggagaccaac atccagagag cccagttcac acagagccaa tggggattca
     5941 aggcagaggg aggcagactg tggataaagt catgggtata ccaaaagaaa cagcagagag
     6001 ggtgggccct gagagagggc cattggagag agaaactgag aaactgctac cagaaagaca
     6061 gacagatgtg acaggagagg aagaattaac caaggggaaa caggacagag aacaaaaaca
     6121 gttgttagct agagacaccc agagacaaga atctgacaaa aatggggaaa gtgcaagtcc
     6181 tgaaagagat agggagagtt tgaaggtaga aattgagaca tctgaggaaa tacaagagaa
     6241 acaagtacag aagcagaccc ttccaagcaa agcatttgag agagaagtag agagaccagt
     6301 agcaaacaga gagtgcgatc cagccgagtt agaagagaag gtgcccaaag tgatcctgga
     6361 gagagataca cagagagggg agccagaggg agggagccag gaccagaaag ggcaggcctc
     6421 cagcccaaca ccagagcctg gggtgggggc gggggacctt ccgggaccta cctcagcccc
     6481 cgtaccttct gggagccagt caggtggaag gggatcccca gtgagcccca ggaggcatca
     6541 gaaaggcctc ctgaattgca agatgccacc tgctgagaag gcttccagga tcagagctgc
     6601 tgagaaggtt tccaggggcg atcaggaatc tccagatgct tgtctgcctc ctgcagtacc
     6661 tgaagcccca gccccacccc aaaagcccct taactctcag agccagaaac atcttgcacc
     6721 tccgcccctt ctttctcccc ttttaccttc tatcaagcca accgttcgta agaccaggca
     6781 agatgggagt caggaagctc cagaggctcc cttgtcctca gagctggagc ctttccaccc
     6841 aaagcctaaa attagaactc ggaagtcctc cagaatgaca ccctttccag ctacctctgc
     6901 tgcccctgag ccccaccctt ccacctccac agcccagcca gtcactccca agcccacatc
     6961 tcaggccact aggagcagga caaataggtc ctctgtcaag acccctgaac cagttgtccc
     7021 cacagcccct gagctccagc cttccacctc cacagaccag cctgtcacct ctgagcccac
     7081 atctcaggtt actaggggaa gaaaaagtag atcctctgtc aagacccctg aaacagttgt
     7141 gcccacagcc cttgagctcc agccttccac ctccaccgac cgacctgtca cctctgaacc
     7201 cacctctcag gctactaggg gaagaaaaaa tagatcctct gtcaagaccc ctgaaccagt
     7261 tgtccccaca gcccctgagc tccagccttc cacctccaca gaccagcctg tcacttctga
     7321 gcccacatat caggctacta ggggaagaaa aaatagatcc tctgtcaaga cccctgaacc
     7381 agttgtgccc acagcccctg agctccggcc ttccacctcc acagaccgac ctgtcacccc
     7441 caagcccaca tctcggacca ctaggagcag gacaaatatg tcctctgtca agacccctga
     7501 aacagttgtc cccacagccc ctgagctcca gatttccacc tccacagacc aacctgtcac
     7561 ccctaagccc acatctcgga ccactaggag caggacaaat atgtcctctg tgaagaaccc
     7621 tgaatcaact gtccctatag cccctgagct cccaccttcc acctccacag agcagcctgt
     7681 cacccctgag cccacatctc gggctactag gggaagaaaa aatagatcct ctggcaagac
     7741 ccctgaaaca cttgtcccca cagcccctaa gctcgagcct tccacttcca cagaccaacc
     7801 tgtcactcct gagcccacat ctcaggccac caggggcagg acaaataggt cctctgtgaa
     7861 gacccctgaa acagttgtcc ccacagcccc tgagctccag ccttccacct ccacagacca
     7921 gcctgttacc cctgagccta cgtctcaggc tactagggga agaacagata gatcctctgt
     7981 caagactcct gaaacagttg tccccacagc ccctgagcta caggcttccg cctccacaga
     8041 ccagcctgtc acctctgagc ccacatctcg gaccactagg ggaagaaaaa atcggtcctc
     8101 tgtcaagacc cctgaaacag ttgtgcccgc agcccctgag ctccagcctc ccacctccac
     8161 agaccgacct gtcacccctg agcccacatc tcgggccact aggggcagga caaataggtc
     8221 ctctgtcaag acccctgaat caattgtccc tatagcccct gagcttcagc cttccacctc
     8281 cagaaaccag cttgtcaccc ctgagcccac atctcgggcc actaggtgca ggacaaatag
     8341 gtcctctgtc aagacccctg agccagttgt ccccacagcc cctgagcccc atcctaccac
     8401 ctccacagac cagcctgtca cccccaagct cacatctagg gccactagga gaaagacaaa
     8461 taggtcctct gtcaagactc ccaaaccagt tgaaccagca gcctctgatc ttgagccttt
     8521 tacccccaca gaccagtccg tcacccctga ggccatagct cagggtggtc agagcaaaac
     8581 actgaggtct tccacagtaa gagctatgcc ggttcctacc acccctgaat tccaatctcc
     8641 tgtcaccaca gaccagccta tttcccctga gcctattact caacccagtt gcatcaagag
     8701 gcagagagcc gctgggaacc ctggctccct cgcagctccc attgaccata agccttgctc
     8761 tgcacccttg gaacctaaat cccaggcctc aaggaaccaa agatggggag cagtgagagc
     8821 agctgaatcc cttacagcca ttcctgagcc tgcctctccc cagcttcttg agacaccaat
     8881 tcatgcctcc cagatccaaa aggtggaacc agcaggtaga tctaggttca ccccggagct
     8941 ccagcctaag gcctctcaaa gccgcaagag gtctttagct accatggatt caccaccaca
     9001 tcaaaaacag ccccaaagag gggaagtctc ccagaagaca gtgattatca aggaagagga
     9061 agaagatact gcagagaagc cagggaagga agaggatgtc gtgactccaa aaccaggcaa
     9121 gagaaagaga gaccaggcag aggaggagcc caacagaata ccaagccgca gcctccgacg
     9181 gaccaaactt aaccaagaat caacagcccc caaagtgctc ttcacaggag tggtggatgc
     9241 tcggggagag cgggctgtgc tggcactggg gggaagtctg gctggttcag cggcagaggc
     9301 ttcccacctg gtcactgatc gcatccgccg gacagtcaag ttcctgtgtg ccctggggcg
     9361 gggaatcccc attctgtccc tggactggct gcatcagtcc cgcaaggctg gtttcttctt
     9421 acccccggat gaatatgtgg tgaccgaccc tgagcaagag aagaactttg gctttagcct
     9481 tcaagacgca ctgagcaggg ctcgggagcg aaggctgcta gagggctatg agatctatgt
     9541 gacccctgga gtccagccac caccacctca gatgggagag attattagct gctgtggagg
     9601 cacataccta cccagcatgc ctcggtccta taagcctcag agagttgtga tcacatgccc
     9661 tcaggacttc cctcattgct ccattccact acgggttggg ctgcccctcc tctcgcctga
     9721 gttcctgctg actggagtgc tgaagcagga agccaagcca gaggcctttg tcctctcccc
     9781 tttggagatg tcatccacct gagaactcca ctaccctttt ccctcccaga ccacgaatta
     9841 gaagatatgt ggaagaaaga actcagggcg ttagaaagga ttggggtata ttgatacaac
     9901 ttgtcctgga acatgggtgg gaccagaaat ctttatgaat aaatgaaaag ataagggatt
     9961 tggaagccac aggttgtttt ttgtttgttt gtttgttttt ttaatggatc tagacccagc
    10021 tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgacaa tcaacctctg
    10081 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
    10141 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
    10201 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
    10261 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
    10321 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
    10381 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
    10441 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
    10501 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
    10561 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
    10621 cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattcgagc tcggtacctt
    10681 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
    10741 gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
    10801 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
    10861 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
    10921 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
    10981 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
    11041 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
    11101 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
    11161 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
    11221 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
    11281 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
    11341 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
    11401 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
    11461 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
    11521 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
    11581 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    11641 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    11701 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    11761 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    11821 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    11881 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    11941 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    12001 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
    12061 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
    12121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
    12181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
    12241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
    12301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
    12361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
    12421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
    12481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
    12541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
    12601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
    12661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
    12721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
    12781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
    12841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
    12901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
    12961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    13021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    13081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    13141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    13201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    13321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    13441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    13501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    13561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    13621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    13681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    13741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    13801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    13861 ccgcctttga
//
