LOCUS       Exported               15249 bp ds-DNA     circular SYN 19-SEP-2024
DEFINITION  dCas9-SunTag_10X_-2A-BFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Sep 20, 2024 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15249
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        835..1164
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1015..1150
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          2298..2363
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2493..2513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2938..3072
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             3240..3873
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3920..4045
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4542..4775
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4960..5004
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5289..5406
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5569..5976
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6030..6050
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      6099..6108
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             6105..10208
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10212..10238
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10257..10277
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10284..10304
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             10383..10439
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10455..10511
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10527..10583
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10599..10655
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10671..10727
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10743..10799
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10815..10871
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10887..10943
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             10959..11015
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11031..11087
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11103..11159
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11175..11231
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11247..11303
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11319..11375
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11391..11447
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11463..11519
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11535..11591
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11607..11663
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11679..11735
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11751..11807
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11823..11879
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             11895..11951
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12039..12095
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             12135..12155
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12165..12221
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             12234..12935
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     LTR             13050..13283
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(13308..13326)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     promoter        14114..14218
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14219..15079
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
      841 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      901 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
      961 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     1021 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     1081 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     1141 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     1201 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     1261 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     1321 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     1381 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     1441 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     1501 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     1561 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     1621 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     1681 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     1741 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     1801 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     1861 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     1921 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     1981 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     2041 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     2101 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     2161 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     2221 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     2281 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     2341 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     2401 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     2461 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     2521 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     2581 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     2641 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     2701 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     2761 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     2821 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2881 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2941 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     3001 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     3061 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     3121 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     3181 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     3241 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     3301 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     3361 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     3421 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     3481 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     3541 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     3601 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     3661 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     3721 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     3781 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     3841 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     3901 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     3961 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4021 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     4081 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     4141 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     4201 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     4261 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     4321 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     4381 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     4441 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     4501 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     4561 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     4621 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     4681 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     4741 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     4801 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     4861 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     4921 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     4981 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     5041 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     5101 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     5161 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     5221 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     5281 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     5341 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     5401 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     5461 gaattatatc gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa
     5521 aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt
     5581 tgcaaggcat ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca
     5641 tgaaaatagc taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc
     5701 cggggccaag aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg
     5761 gtccccagat atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct
     5821 cccccaagga cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc
     5881 gcttctgttc gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc
     5941 ggcgcgccag tcctccgaca gactgagtcg cccggggggg atctggagct ctcgagaatt
     6001 ctcacgcgtc aagtacgcgc gccaccatgc ccaagaagaa gcgcaaggtg ggacgcgtct
     6061 gcaggatatc aagcttgcgg taccgcgggc ccgggatcgc caccatggac aagaagtaca
     6121 gcatcggcct ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca
     6181 aggtgcccag caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga
     6241 acctgatcgg cgccctgctg ttcgacagcg gagaaacagc cgaggccacc cggctgaaga
     6301 gaaccgccag aagaagatac accagacgga agaaccggat ctgctatctg caagagatct
     6361 tcagcaacga gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc
     6421 tggtggaaga ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg
     6481 tggcctacca cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca
     6541 ccgacaaggc cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg
     6601 gccacttcct gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca
     6661 tccagctggt gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg
     6721 tggacgccaa ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga
     6781 tcgcccagct gcccggcgag aagaagaatg gcctgttcgg caacctgatt gccctgagcc
     6841 tgggcctgac ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc
     6901 tgagcaagga cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt
     6961 acgccgacct gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc
     7021 tgagagtgaa caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg
     7081 acgagcacca ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga
     7141 agtacaaaga gattttcttc gaccagagca agaacggcta cgccggctac atcgatggcg
     7201 gagccagcca ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca
     7261 ccgaggaact gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg
     7321 acaacggcag catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc
     7381 aggaagattt ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct
     7441 tccgcatccc ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga
     7501 ccagaaagag cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg
     7561 ccagcgccca gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga
     7621 aggtgctgcc caagcacagc ctgctgtacg agtacttcac cgtgtacaac gagctgacca
     7681 aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa
     7741 aagccatcgt ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag
     7801 aggactactt caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc
     7861 ggttcaacgc ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact
     7921 tcctggacaa tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt
     7981 ttgaggacag agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca
     8041 aagtgatgaa gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc
     8101 tgatcaacgg catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg
     8161 acggcttcgc caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag
     8221 aggacatcca gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca
     8281 atctggccgg cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg
     8341 agctcgtgaa agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag
     8401 agaaccagac cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag
     8461 agggcatcaa agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc
     8521 tgcagaacga gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc
     8581 aggaactgga catcaaccgg ctgtccgact acgatgtgga cgctatcgtg cctcagagct
     8641 ttctgaagga cgactccatc gataacaaag tgctgactcg gagcgacaag aaccggggca
     8701 agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcgccagc
     8761 tgctgaatgc caagctgatt acccagagga agttcgacaa tctgaccaag gccgagagag
     8821 gcggcctgag cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc
     8881 agatcacaaa gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga
     8941 acgacaaact gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt
     9001 tccggaagga tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg
     9061 acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa
     9121 gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg
     9181 agcaggaaat cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt
     9241 tcaagaccga gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa
     9301 acggcgaaac aggcgagatc gtgtgggata agggccggga ctttgccacc gtgcggaaag
     9361 tgctgtctat gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca
     9421 gcaaagagtc tatcctgccc aagaggaaca gcgacaagct gatcgccaga aagaaggact
     9481 gggaccctaa gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg
     9541 tggccaaagt ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga
     9601 tcaccatcat ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg
     9661 gctacaaaga agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc
     9721 tggaaaacgg ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac
     9781 tggccctgcc ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga
     9841 agggctcccc cgaggataat gagcagaaac agctgtttgt ggaacagcac aaacactacc
     9901 tggacgagat catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta
     9961 atctggacaa ggtgctgagc gcctacaaca agcacagaga caagcctatc agagagcagg
    10021 ccgagaatat catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt
    10081 actttgacac caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca
    10141 ccctgatcca ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg
    10201 gaggcgacgc ctatccctat gacgtgcccg attatgccag cctgggcagc ggctccccca
    10261 agaaaaaacg caaggtggaa gatcctaaga aaaagcggaa agtggacggc attggtagtg
    10321 ggagcaacgg cagcagcgga tccaacggtc cgggtggatc tggaggtgga ggttctggag
    10381 gagaagaact tttgagcaag aattatcatc ttgagaacga agtggctcgt cttaagaaag
    10441 gttctggcag tggagaagaa ctgctttcaa agaattacca cctggaaaat gaggtagcta
    10501 gactgaaaaa ggggagcgga agtggggagg agttgctgag caaaaattat catttggaga
    10561 acgaagtagc acgactaaag aaagggtccg gatcgggtga ggagttactc tcgaaaaatt
    10621 atcatctcga aaacgaagtg gctcggctaa aaaagggcag tggttctgga gaagagctat
    10681 tatctaaaaa ctaccacctc gaaaatgagg tggcacgctt aaaaaaggga agtggcagtg
    10741 gtgaagagct actatccaag aattatcatc ttgagaacga ggtagcgcgt ttgaagaagg
    10801 gttccggctc aggagaggaa ctgctctcga agaactatca tcttgaaaat gaggtcgctc
    10861 gattaaaaaa gggatcgggc agtggtgagg aactactttc aaagaattac cacctcgaaa
    10921 acgaagtagc tcgattaaag aaaggttcag ggtcgggtga agaattactg agtaaaaatt
    10981 atcatctgga aaatgaggta gcgagactaa aaaaggggag tggttctggc gaagagttgc
    11041 tatcgaaaaa ttatcatctt gagaacgaag ttgctaggct caaaaagggc tcaggctcag
    11101 gcgaggagtt gctctcgaaa aactaccact tggaaaatga ggtcgcgagg ttgaaaaagg
    11161 ggagcgggtc gggcgaggag ttattgagca aaaactatca tttagagaac gaagtcgcgc
    11221 gcttaaagaa aggctcgggc tcgggcgaag aactcttatc gaagaactac cacctcgaaa
    11281 atgaggtcgc caggttgaaa aagggcagtg gcagcgggga ggaactcttg agcaagaact
    11341 accacttgga gaatgaggtc gcgagattga agaaagggtc ggggagcggc gaggaattgc
    11401 tcagcaagaa ttatcatttg gagaacgaag tcgccaggct caagaaaggc tcggggtcgg
    11461 gggaggaatt gttgagtaaa aactaccact tggaaaatga agtcgccagg ctcaaaaaag
    11521 ggagtgggag cggcgaagag ttattgagca aaaattacca cttggagaac gaagtggcaa
    11581 ggctcaagaa agggagcggc agcggggagg agctcttatc gaagaactac cacttagaga
    11641 atgaagtcgc ccgcttgaag aaaggctcgg ggagcgggga agagctcttg agcaagaact
    11701 accacttgga aaatgaggtg gcgcgcttga agaaagggag cgggagcggg gaagagttac
    11761 tatctaagaa ttatcatctc gagaacgagg tggctcgact aaagaagggc tccggcagtg
    11821 gggaggaact cctgtcgaag aactatcatc ttgaaaatga ggttgcaaga cttaaaaagg
    11881 ggtccggatc aggtgaggaa ctactcagta agaattacca cctggaaaac gaagttgcac
    11941 gtttgaagaa aggatcagga tcaggcgaag aactgctctc aaaagattat catttggaaa
    12001 atgaggttgc acgtttaaaa aagggaagtg gcagtggtga ggaacttctg tcgaaaaatt
    12061 atcatctcga gaatgaagta gcccgactta aaaagggttc tggctcgggt cagcggccgc
    12121 aaggtggagg tggacccaag aagaagcgca aggtgggaag cggagctact aacttcagcc
    12181 tgctgaagca ggctggagac gtggaggaga accctggacc tgcaccagga agtatgagcg
    12241 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
    12301 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
    12361 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
    12421 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
    12481 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
    12541 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
    12601 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
    12661 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
    12721 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
    12781 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
    12841 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
    12901 acctccctag caaactgggg cacaagctta attaagcggc cgcgactcta gagtcgacct
    12961 gcagggatcg atcactgagg tacctttaag accaatgact tacaaggcag ctgtagatct
    13021 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
    13081 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
    13141 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    13201 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    13261 gtcagtgtgg aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac
    13321 ctaaatcgta tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac
    13381 aatatgtaca agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc
    13441 tcgtaaactg ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc
    13501 gtcccgcacc cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc
    13561 tacgccggac gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat
    13621 atcgccgaca tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt
    13681 ttcggcgtgg gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg
    13741 catgcaccat tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc
    13801 ctaatgcagg agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc
    13861 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg
    13921 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
    13981 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg
    14041 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
    14101 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
    14161 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
    14221 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
    14281 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
    14341 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
    14401 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
    14461 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
    14521 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
    14581 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
    14641 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
    14701 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
    14761 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
    14821 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
    14881 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
    14941 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
    15001 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
    15061 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
    15121 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
    15181 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
    15241 aggatcttc
//
